ContextAgroecological practices are known to reduce environmental pressure of farming systems and increase food system resilience in tropical regions. In contrast, in the temperate climate and industrialized agricultural context of the Netherlands, agroecology use remains limited and its impacts are unknown. As agroecological systems can form a sustainable alternative to conventional farming, it is relevant to study to what extent farmers use agroecological practices in the Netherlands, potentially serving as a model for transition. ObjectiveThis study assesses spatial patterns of uptake of agroecological practices and their relationship with income resilience. MethodsUsing data from 735 Dutch farms, we created a composite Agroecology Index to quantify the extent of use of agroecological practices. The FAO's 10 Elements of Agroecology framework was used to select indicators for the index, by selecting the six of the Elements in this framework for which our dataset contained suitable data. Linear regressions explored underlying factors, such as farm size and farm type. Using an outlier analysis, bright and dark spot farms, exceeding or lagging the Dutch average, were mapped. We evaluated the effects of agroecological practices on income resilience over a 10-year period. Results and conclusionsOn a 0–100 scale, agroecology scores ranged from 16.5% to 61.1% (36.6 ± 7.0), with stark contrasts between different Elements of agroecology. Arable farms excelled in Diversity, while livestock farms performed better in Efficiency. Spatial variation was substantial, with both bright and dark spots dispersed across the country. We found highest average scores and the strongest prevalence of bright spots in the province of Flevoland, an area with an exceptional number of organic farmers. Higher agroecology scores were linked to increased income stability, independent of farm size or type. SignificanceThe methods developed in this study provide an approach to address the growing demand for evidence of the upscaling of agroecology in practice. Also, the relationships between agroecology and social and environmental outcomes from farm to regional scales can be addressed, which can guide developments towards sustainable agriculture.