For rapid and effective detection of airborne microorganisms, it is preferable to remove dust particles during the air sampling process because they can reduce the detection accuracy of measurements. In this study, a methodology of real-time separation ofaerosolized Staphylococcus epidermidis (S. epidermidis) andpolystyrene latex (PSL) particles of similar size was investigated. These two species represent biological and non-biological particles, respectively. Due to their different relative permittivities, they grasp different numbers of air ions under corona discharge. After these charged particles enter a mobility analyzer with airflow, in which an electric field is applied perpendicular to the airflow, the S. epidermidis and PSL particles separate, due to the difference in their electric mobilities, and exit through two different outlets. Purities and recoveries for S. epidermidis and PSLat their respective outlets were determined with measurements of aerosol number concentrations and ATP biol...