Passive acoustics monitoring (PAM) has been used to detect crustacean feeding activity. The aim of the present study was to evaluate the sound emission of the giant freshwater prawn Macrobrachium rosenbergii during feeding a commercial pelleted food in captivity, as well as described the sound generation mechanism and main acoustic variables for the first time. The structure responsible for sound emission was identified based on simultaneous audio and video recordings. Fourteen animals divided into three size classes were recorded individually. The acoustic parameters (sound duration, minimum, maximum and peak frequency and sound pressure level) of 80 sound pulses were analysed for each size classes. The giant freshwater prawn emits click sound when closing the mandibles to shred the food, as they collide with each other. Minimum and peak frequency were similar among the size classes, while the other acoustic parameters were significantly different, but they did not follow an apparent pattern in relation to the size of the prawns. The M. rosenbergii emits sound during feed consumption, and the acoustic parameters obtained in the present study could be useful to monitor feeding activity in prawn farming.