Duration Of Healing Research Articles

Incidence and Management of Skin Lesions and Minor Wounds in Chronic Obstructive Pulmonary Disease Patients undergoing Advanced Bronchodilator Therapy

AbstractWhile triple inhalation therapy is commonly employed in the treatment of chronic obstructive pulmonary disease (COPD), limited knowledge exists regarding its impact on the healing of minor wounds and integrity of the epidermis. This study investigated the impact of combining triple inhalation therapy with double bronchodilators on the aforementioned parameters in patients with stable COPD. A cross‐sectional study was conducted in Changzhou, China, from March 2022 to October 2023, involving 540 patients who had received the diagnosis of stable COPD. Combined therapy participation requirements stipulated a minimum of 6 months of uninterrupted treatment. Dermatological examinations, demographic data and clinical records were utilized to collect information regarding the elasticity, moisture content and duration of wound healing. The research revealed that there were no statistically significant differences in demographic and clinical characteristics between the groups that received triple inhalation alone and triple inhalation in combination with double bronchodilators (p > 0.05). Nevertheless, the combined therapy group demonstrated shorter duration since receiving a diagnosis of COPD (p < 0.05). It is noteworthy to remark that the combined therapy group exhibited significantly higher skin moisture content and shorter interval for wound recovery when compared with the group that only received triple inhalation (p < 0.05). Lung function measurements in combined therapy group indicated enhanced Forced expiratory volume in the first second (FEV1) and FEV1/forced vital capacity ratio, as well as a reduction in COPD exacerbations. Nevertheless, no statistically significant distinction in skin elasticity was observed among the groups (p > 0.05). The supplementary application of triple inhalation therapy and double bronchodilators in stable COPD patients not only improved respiratory outcomes but also positively impacted skin health, specifically by promoting wound healing and augmenting moisture levels. These results highlighted the possible benefits of implementing a holistic treatment approach in COPD, suggesting that incorporating these therapies could offer additional advantages to the epidermis.

Assessing the Efficacy of Laser Therapy and Autologous Platelet-Rich Plasma (PRP) Treatment for Chronic Wounds.

(1) Background: The management of persistent wounds is a topic of significant concern, particularly when they become chronic. Clinicians are focused on reducing the healing duration of chronic wounds and employing the most efficient treatments. Successful wound management requires an understanding of standard healing processes, the elements that can impede healing progress, and strategies to manage these impediments. (2) Methods: We participated in a study involving a cohort of 115 patients. Data were collected through subjective assessments via questionnaires, examining the comparative effects of laser therapy and platelet-rich plasma (PRP) on patients' conditions and monitored the progression of chronic wounds. For the study, we utilized a branded laser equipped with a 12-watt probe to stimulate chronic wounds in 65 patients, while the PRP procedure was administered to 50 patients. (3) Results: We observed a greater improvement in local symptoms among the patients who received laser therapy compared to those in the PRP group. (4) Conclusions: We consider both therapies to be of significant importance due to their positive and beneficial effects, particularly on the symptomatology and progression of chronic wounds. Nevertheless, superior results were documented in patients who underwent laser therapy.

Application of robot navigation system for insertion of femoral neck system in the treatment of femoral neck fracture

PurposeTo evaluate the short-term clinical efficacy and advantages of surgery robot positioning system for insertion of Femoral Neck System (FNS) in the treatment of femoral neck fractures.MethodsThe clinical data of 52 patients with Femoral neck fracture (FNF) who had been treated with FNS between June 2020 and September 2021 were retrospectively analyzed. Among them, 26 patients were treated with traditional FNS (control group), while 26 additional patients were treated with FNS assisted by an orthopaedic robot positioning system (study group). The operation duration, frequency of key-guide needle placement, intraoperative blood loss, incision length, fracture healing rate, fracture healing time, and the Harris scores at the last follow-up were calculated and compared between the 2 groups.ResultsThe study group had shorter operation duration, fewer numbers of placing the key-guide needle, less intraoperative blood loss, and smaller surgical incisions than the control group (all, P < 0.05). There was no significant difference in the rate of fracture healing rate between the 2 groups (P = 0.47), while the fracture healing duration of the study group was shorter than that of the control group (P = 0.03). At the last follow-up, compared with the control group, the Harris score and the number of excellent and good ratings were significantly higher in the study group (all, P < 0.05).ConclusionsUsing orthopaedic surgery robot positioning system-assisted FNS in the treatment of FNFs can effectively improve the efficiency of surgery, shorten operation time, and reduce the number of placing the key-guide needle, intraoperative blood loss, and operative trauma. Simultaneously, it shortens the duration of fracture healing and improves the recovery of hip function.

Efficacy and Safety of Propolis for Treating Recurrent Aphthous Stomatitis (RAS): A Systematic Review and Meta-Analysis.

The systematic review assessed the efficacy and safety of propolis for treating recurrent aphthous stomatitis (RAS). The review adopted the PICO framework to examine the effects of topical and systemic propolis on RAS while also comparing it to established treatments, placebos, or no treatment. The main focus was on the healing time, pain levels, adverse effects, the likelihood of ulcer recurrence, and accompanying symptoms such as redness. The team included randomised controlled trials (RCTs) and quasi-randomised trials, excluding case reports and studies on oral ulcers other than RAS. In May 2022, the review team comprehensively searched nine databases and trial registries following the PRISMA guidelines. The protocol was registered in the PROSPERO database under the registration number CRD42022327123. Two review authors conducted a comprehensive and autonomous search for pertinent papers and extracted essential data. Where data permitted, the team utilised Review Manager 5 to conduct a random-effects meta-analysis, assessing the risk of bias and heterogeneity of the included studies. Where possible, the GRADE Pro programme was used to assess the certainty of the evidence for all the outcomes. This review included 10 RCTs, comprising 825 participants aged between 18 and 69 years. Seven studies evaluated the efficacy and safety of propolis when applied topically, all of which used different formulations, concentrations, and carriers. The remaining three studies assessed systemic administration in tablet form. The duration of investigations ranged from 5 days to 3 years. The review team classified two studies as having an overall 'high risk' of bias, while the remaining studies were categorised as having an overall 'uncertain risk'. The overall certainty of the evidence was 'very low'. The results indicate that topical and systemic propolis may decrease the duration of healing, alleviate pain, and reduce redness in patients with RAS compared to a placebo. However, the certainty of the evidence is very low. These may be due to the high risk of bias, substantial heterogeneity, and limited sample sizes in the included studies. For these reasons, the results of this review should be interpreted with caution. Nevertheless, the limited number of adverse effects observed suggests that propolis may have a favourable safety profile when used for a short period in treating RAS.

The Effect of Ketorolac on Bone Healing Rate Following Osteotomy in Pediatric Patients with Deformity or Limb-length Discrepancy

Abstract Background: The mechanism of action of nonsteroidal anti-inflammatory drugs (NSAIDs) and the results of testing in some animal fracture models have created concerns with bone healing when these drugs are used for perioperative orthopedic analgesia. Given that distraction osteogenesis patients require new bone to be formed, they would potentially be sensitive to the effects of NSAIDs such as Ketorolac. We sought to determine the relationship between the perioperative use of Ketorolac and the duration of healing in pediatric patients undergoing distraction osteogenesis for either limb lengthening or deformity correction surgery. Materials and Methods: A single-center retrospective chart review of 94 pediatric patients aged 17 years or younger who had undergone surgery for limb lengthening, deformity correction, or a combination of limb lengthening and deformity correction was carried out. We collected and analyzed patient demographics, details of surgery and pathology, perioperative pain management, duration of healing, and incidence of complications in this cohort of patients. Results: Patients were divided into three groups: a deformity correction group (n = 46), a limb lengthening group (n = 30), and a combined deformity correction and limb lengthening group (n = 18). The mean magnitude of deformity was 21°. Patients in the lengthening group had a mean lengthening of 4.1 cm. Patients in the combined group had a mean magnitude of deformity of 14.7° and a mean lengthening of 3.8 cm. Patients in all three groups received an average of 4 doses of ketorolac in the perioperative period. We found no correlation between the total amount of ketorolac used and the duration of healing among patients who had deformity correction, limb lengthening, or a combination of deformity correction and limb lengthening surgery (P = 0.82, 0.20, and 0.18, respectively). The correlation results also showed no sex variations in either group. Conclusions: Ketorolac can safely be used as an analgesic in pediatric patients undergoing distraction osteogenesis for limb lengthening, deformity correction, or a combination of both procedures.

Defining the end point of drug therapy in tuberculosis of spine: Analysis of 76 cases in a tertiary care centre

Abstract Background: The duration of chemotherapy for the tubercular spine is an unresolved issue. Various drug regimens have been used for various durations. Objectives: (1) To calculate the duration of antitubercular therapy (ATT) for achieving the healed status of the tubercular spine, (2) to report the behavior of kyphotic deformity, and (3) outcome of neurological deficit. Materials and Methods: This longitudinal descriptive study included 76 cases of tubercular spine diagnosed on clinico-imaging and/or on histopathology/molecular tests. They were treated as per the DOTS regime. Surgery was done for predetermined indications. ATT was stopped once healed status was demonstrated on CE-MRI/PET-CT. Kyphotic deformity was measured by modified Konstam’s method, and neurological grading was assessed by Tuli’s grading. Results: The mean duration of healing was 20.08 ± 8.09 months (range 9–48 months) in primary spinal TB with no recurrence after the mean follow-up of 15 months (range 3–108 months). Overall, 92.6% showed good neural outcomes at the final follow-up. Mean kyphotic deformity was found to be 16.75° ± 12.23°, 18.78° ± 12.62°, and 18.78° ± 12.53° at presentation, after stopping ATT and at the final follow-up. Conclusion: It is unscientific to stop ATT in spinal TB till healed status is demonstrated by contrast-enhanced MRI or PET-CT. Although the sample size is small, the data is enough to prove this point that if all patients were treated till healed status was achieved, there would be no recurrence.

Evaluation of autologous platelet rich fibrin in chronic non-healing leg ulcers

Context: Chronic non healing leg ulcers cause psychological, social, and financial burden to the patient. Platelet rich fibrin (PRF), a biological membrane with plethora of growth factors acts as a cheaper and simpler option for the management of non-healing leg ulcers. Aims: To evaluate the effectiveness of PRF in the treatment of chronic nonhealing leg ulcers of varying etiologies. Material and Methods: It was a prospective study conducted in 30 patients in a tertiary care center. PRF dressing was done weekly for the chronic non healings ulcers of various etiologies for 6 weeks. Response to therapy was assessed by calculation of wound area and volume and digital photographs before each treatment session. Statistical analysis was done using Chi-square test, paired t-test and ANOVA test to get P values. Results: In our study, the mean age of subjects was 55.9 ± 14.08. 21 (70%) subjects were male and 9 (30%) were female. Majority of the ulcers were trophic ulcers due to hansen’s disease (n = 11, 36.7%), followed by diabetic ulcers (n = 9, 30%), venous ulcers (n = 6, 20%) and traumatic ulcers (n = 4, 13.3%). Percentage decrease of mean ulcer area and volume were 92.3% and 97.6%, respectively. There was significant decrease in the mean area and volume (P &lt; 0.0001) of the ulcer from 2nd week onwards till the end of 6th week. Conclusions: PRF can be used as an adjuvant along with standard treatment in the management of chronic non healing ulcers to reduce the duration of healing.

Experimental Research on the Influence of Ion Channels on the Healing of Skin Wounds in Rats

At the level of skin wounds, an electrical potential difference develops between the edges of the wound and the center of the wound, which favors the migration of cells in the process of their healing. Cells migrate in an electric field because they have a certain electrical membrane potential. This potential is due to differences in the transmembrane electrochemical gradient. The transmembrane electrochemical gradient is due to the migration of sodium, potassium, and calcium ions into the corresponding ion channels. If this is the case, the modification of the functionality of these ion channels should influence the membrane potential and, as a consequence, the wound healing process. In this experiment, we set out to investigate whether the chemical manipulation of ion channels by amiodarone influences the wound healing process. Amiodarone blocks several types of ion channels, but at different concentrations: at low concentrations, it blocks only potassium channels; at medium concentrations, potassium and calcium channels; and at high concentrations, it blocks potassium, calcium, and sodium channels. We worked on rats that were given experimental skin lesions and evaluated the influence of the healing of these lesions upon the topical administration of amiodarone in three concentrations, 200 nM, 2000 nM and 200,000 nM, compared to an untreated group and a group treated with benzyl alcohol, the amiodarone solvent. In our experimental conditions, low concentration amiodarone promoted wound healing both in terms of duration of healing and also in terms of speed of healing. This means that blocking some ions, possibly potassium channels, might promote wound healing.

Accelerated wound healing after topical application of hyaluronic acid cotton to hemorrhoidectomy wounds in a rat model.

Anal wounds following hemorrhoidectomy can lead to severe pain and postoperative bleeding, impacting patient recovery and quality of life. Hyaluronic acid (HA) stimulates tissue regeneration and wound healing by accelerating cell migration and proliferation. This study aimed to investigate the differences in wound healing rate and completeness of recovery of perianal wounds topically treated with HA-soaked cotton in a murine model. Forty-eight 8-week-old Sprague-Dawley rats with perianal wounds created using a biopsy punch were divided into 2 groups: simple dressing with gauze (control) and topical HA-soaked cotton. A single application of HA-soaked cotton was administered after surgery. Wound healing rate and completeness of recovery were evaluated by measuring the healed area and conducting histological analyses. The HA-cotton group exhibited a shorter complete wound healing duration compared to the control group (13.9 days vs. 16.4 days, P = 0.031). Differences in wound healing area between the 2 groups were greatest on postoperative day 2 (51.6% vs. 28.8%, P < 0.001). The HA-cotton group exhibited fewer cases of granulation tissue (2 vs. 5) or redness (0 vs. 3) upon complete wound healing. Histologically, the HA-cotton group showed accelerated reepithelialization, rapid shift to lymphocyte-dominant inflammation, enhanced fibroblast proliferation, and increased collagen deposition compared to the control group. Herein, topical application of HA-soaked cotton on perianal wounds in rats resulted in accelerated wound healing, particularly in the initial stages, and improved completeness of recovery, underscoring the potential of the topical application of HA-soaked cotton on hemorrhoidectomy wounds in human patients to improve wound healing.

Effects of healing start time and duration on conventional and high-performance concretes incorporating SAP, crystalline admixture, and sepiolite: A comparative study

This research investigates the self-healing capability of conventional and high-performance concrete containing either a superabsorbent polymer (SAP), a crystalline admixture (CA), or water-encapsulated in sepiolite by quantifying the recovery of water tightness through the water permeability test and chloride permeability via cracks and matrix penetration on healed specimens. Specimens were pre-cracked to a crack width range of 50–450 µm at 28 days. Specimens were healed under water in four time combinations: healing starting at the concrete age of 28 days or 56 days and a healing duration of 28 or 56 days. Additionally, a healing condition of presaturation during 1 day of water immersion and storage in a humidity chamber for 27 days was studied. The results show that delayed healing reduced the self-healing efficiency, and extending the duration to 56 days enhanced healing, especially for narrow cracks (≈100 µm), and reduced chloride permeability. Specimens with SAP showed superior early-stage healing, but low delayed healing. Sepiolite enhanced healing for delayed cracks with variable effectiveness. CA improved healing for both early and delayed healing, exhibiting low chloride penetration in healed specimens.

Wound healing after therapy of oral potentially malignant disorders with a 445-nm semiconductor laser: a randomized clinical trial

ObjectivesOral potentially malignant disorders (OPMDs) are the most clinically relevant precursor lesions of the oral squamous cell carcinoma (OSCC). OSCC is one of the 15 most common cancers worldwide. OSCC is with its high rate of mortality an important cause of death worldwide. The diagnosis and therapy of clinically relevant precursor lesions of the OSCC is one of the main parts of prevention of this malignant disease. Targeted therapy is one of the main challenges concerning an oncologically safe tissue removal without overwhelming functional and aesthetic impairment.Materials and methodsIn this randomized controlled trial, a newly introduced intraoral 445-nm semiconductor laser (2W; cw-mode; SIROLaser Blue, Dentsply Sirona, Bensheim, Germany) was used in the therapy of OPMDs. Duration and course of wound healing, pain, and scar tissue formation were compared to classical cold blade removal with primary suture by measuring remaining wound area, tissue colorimetry, and visual analogue scale. The study includes 40 patients randomized using a random spreadsheet sequence in two groups (n1 = 20; n2 = 20).ResultsThis comparative analysis revealed a significantly reduced remaining wound area after 1, 2, and 4 weeks in the laser group compared to the cold blade group (p < 0.05). In the laser group, a significantly reduced postoperative pain after 1 week was measured (p < 0.05).ConclusionLaser coagulation of OPMDs with the investigated 445-nm semiconductor laser is a safe, gentle, and predictable surgical procedure with beneficial wound healing and reduced postoperative discomfort.Clinical relevanceCompared to the more invasive and bloody cold blade removal with scalpel, the 445-nm semiconductor laser could be a new functional less traumatic tool in the therapy of OPMDs. The method should be further investigated with regard to the identification of further possible indications.Trail registrationGerman Clinical Trials Register No: DRKS00032626.

How successful is synthetic graft treatment for children with pathological hip fractures?

To determine whether synthetic grafts are a satisfactory treatment option for pathological proximal femoral fractures in children. Paediatric patients treated for pathological fractures of the proximal femur between 2013 and 2020 were evaluated retrospectively. 17 patients with a mean age of 10.7 years (range 6-16 years) were assessed. The definitive histopathological diagnoses were SBC (simple bone cyst) (12) and ABC (aneurysmal bone cyst) (5). The median duration of follow-up was 37 months (range 12-70 months). All patients returned to their normal daily routine within 3-8 months following surgery. The mean post-op recovery time was 3.2 months (range 3-6 months). Graft was incorporated at approximately 12 months. No significant radiographic healing was observed in 2 patients. In the remaining 15 patients, the mean duration of healing was 14 months (range 8-24 months). Synthetic grafts are a satisfactory treatment option for pathological proximal femoral fractures in children.

The Duration of Bone Healing and Nonunion Ratio After Ulnar Shortening Osteotomy Using a 5-Hole Forearm Compression Plate With Transverse Osteotomy.

Ulnar shortening osteotomy using various osteotomy sites, osteotomy methods, and surgical techniques with a variety of implants has been reported, but nonunion rates and the duration of bone healing are not uniform by the authors. The purpose of this study was to investigate the duration of bone healing and nonunion ratio in patients who underwent ulnar shortening osteotomy by a 5-hole nonlocking plate with a simple transverse osteotomy for ulnar impaction syndrome and to determine the correlation between the gap length of the osteotomy site and the duration of bone healing. We assessed patients who underwent ulnar shortening osteotomy for ulnar impaction syndrome using a 5-hole nonlocking plate fixation followed by a simple transverse osteotomy between July 2012 and October 2021. This study was a case series study, and the level of evidence was IV. A total of 80 patients were included. The mean age of the patients was 46.7 years, and 46 patients were men. The average bone union period was 41.8 ± 18.7 weeks. The correlation between the gap length of the osteotomy site and the duration of bone union of the osteotomy site was not significant. The nonunion ratio of our method was 2.5%. The nonunion rate of our ulnar shortening osteotomy with a simple transverse osteotomy and a 5-hole nonlocking plate was comparable to that in previous reports, but our method required slightly longer periods for bone union. There was no correlation between the gap length of the osteotomy and the duration of bone union. Level IV (Case series).

The impact of ulinastatin on wound infection and healing in patients with burn wounds: A meta-analysis.

Burn injuries result in localised tissue damage and precipitate systemic responses; routine clinical treatments, which typically include metabolic nutritional support and anti-infection therapies, do not yield optimal outcomes. Therefore, we aimed to systematically evaluate the effects of ulinastatin on wound infection and healing in patients with burns to provide reliable evidence-based recommendations for burn treatment. An electronic search of the Web of Science, PubMed, Cochrane Library, Embase, Wanfang, Chinese Biomedical Literature Database, and China National Knowledge Infrastructure databases, supplemented by manual searches, was conducted from database inception to October 2023 to collect randomised controlled trials (RCTs) assessing the efficacy of ulinastatin for the treatment of burns. Two researchers screened all retrieved articles according to the inclusion and exclusion criteria; the included studies were evaluated for quality, and the relevant data were extracted. Stata 17.0 software was employed for data analysis. Overall, 8 RCTs with 803 patients were included, with 404 and 399 in the ulinastatin and conventional treatment groups, respectively. The analysis revealed that wound infections (odds ratio [OR] = 0.08, 95% CI: 0.02-0.35, p = 0.001) and complications (OR = 0.21, 95% CI: 0.10-0.42, p < 0.001) were significantly lower, and wound healing time (standardised mean differences [SMD] = -1.31, 95% CI: -2.05 to -0.57, p = 0.001) was significantly shorter, in the ulinastatin groups than in the control group. This meta-analysis revealed that ulinastatin can effectively reduce the incidence of wound infections and complications and significantly shorten the duration of wound healing in patients with burns, thereby promoting early recovery in these patients.

Faktor-Faktor Yang Berhubungan Dengan Proses Penyembuhan Luka Post SC Di Rumah Sakit Permata Medika Kota Semarang

The maternal mortality rate of post-SC infection reaches 7.3%, and 90% causes postpartum morbidity. This study aimed to determine the factors associated with the SC wound healing process. This study used a quantitative method, a type of analytic survey research with a cross-sectional design approach. The population of this study were all post-SC mothers in the postpartum ward of Permata Medika Hospital. The sampling technique used was a consecutive sampling of 34 respondents. The research instrument was a questionnaire. Data analysis was done using univariate and bivariate (chi-square test, alpha = 0.05). The results of this study showed that there was a relationship between the SC wound healing process and early mobilisation (p-value 0.000 &lt; 0.05), which means there was a relationship between the SC wound healing process and nutritional status (BMI) (p-value 0.04 &lt; 0.05), and there was a relationship between the SC wound healing process and anaemia (p-value = 0.00 &lt;0.05) because if p-value &lt; 0.05, then Ha is accepted. Ho is rejected, meaning a significant relationship exists between early mobilisation, nutritional status, and anaemia in SC wound healing. Many factors affect the SC wound healing process, three of which are early mobilisation factors, which, if done before 6–8 hours after SC, has more potential to accelerate SC wound healing; normal nutritional status as an expression of a person's nutritional balance, which plays a role in the process of body tissue growth and has an impact on the duration of wound healing; and anaemia, which anaemia is a symptom of low haemoglobin levels in the blood and obstructs oxygen flow and prevents the growth and repair of tissue from the SC surgical incision.

Open reduction and internal fixation of modified Mason type III/IV radial head fractures with more than three fragments: an analysis of the clinical outcome and reoperation rate

PurposeIt is generally accepted that a radial head fracture (RHF) with more than three parts is not suitable for repair; therefore, most authors suggest straightforward radial head arthroplasty (RHA). With up to 20% risk for reoperation after RHA, improvement in reduction and fixation techniques may represent a valuable alternative before further extending the indications for arthroplasty. To determine the functional results and radiological failure rate after osteosynthesis of multi-fragmentary RHF with more than three articular fragments. We specifically determined (1) the one-year Broberg and Morrey functional elbow score, (2) duration of fracture healing, (3) complication rate, and (4) number of patients converted to RHA.MethodsThis study is a retrospective single-center case series. All patients who underwent primary osteosynthesis for RHF between 2012 and 2019 were included. Nine patients with an average age of 52 years had an average clinical and/or radiological follow-up of 49 months.ResultsThe preoperative imaging identified nine fractures with four fragments. Three patients underwent osteosynthesis with plates and screws, whereas six patients underwent osteosynthesis with only screws. The mean Broberg and Morrey score was 95 points. Overall, eight of the nine patients had satisfactory results. All patients retained their radial heads and showed radiological fracture healing. Only two patients presented with low-grade complications requiring no further surgery.ConclusionOur study showed that osteosynthesis of RHF with up to four fragments can achieve good functional results with a low complication rate and seems to be a valid alternative to RHA.

Вміст факторів росту та фактора 1α, індукованого гіпоксією, в рановому ложі шкіри щурів із метаболічним синдромом

Minor injuries in healthy people usually heal well, but larger wounds or the presence of various physiological (age) or pathological conditions (metabolic syndrome, obesity, diabetes, and cancer) can impede this process. The aim of our work was to determine the factors that may influence the duration of healing (growth factors and hypoxia-induced factor 1α) in the wound bed of rats with metabolic syndrome. The experiments were conducted on 80 white non-linear laboratory rats, aged 4-5 months, which were divided after birth into 2 groups of 40 animals each (20 males and 20 females). Group I rats were subcutaneously injected with saline at a dose of 8 μg/ ml on days 2, 4, 6, 8, and 10 after birth. Group II rats were administered a sodium glutamate solution at a dose of 4.0 mg/ kg at the same time. At the age of 4 months, animals of both subgroups were modeled with incised wounds . The control animals were rats in each of the groups in which wounds were not modeled. The material for biochemical studies was the skin in the areas of the former wound bed. Rats in the control group had their skin excised at the same sites as those in the experimental groups. The skin was homogenized and the content of growth factors of endothelial and nerve cells (VEGF, NGF, respectively) and hypoxia-inducible factor (HIF-1α) was determined by immuno-enzymatic method. In unoperated male rats with metabolic syndrome, the skin content of VEGF, NGF, and HIF-1a increased compared to control animals without the syndrome. In unoperated females with metabolic syndrome, VEGF levels decreased with a simultaneous increase in NGF and HIF-1α. In the wound bed of animals with metabolic syndrome, after the closure of the wound surface, the content of VEGF and HIF-1α increased, and the content of NGF remained unchanged compared with the values in unoperated rats. The results obtained indicate the involvement of growth factors VEGF and NGF and HIF-1α in prolonging the duration of healing of incised wounds in rats with metabolic syndrome. At the same time, these growth factors and HIF-1α may be involved in the mechanisms of development of some postoperative complications.

Calcium oxide/silica nanocomposite and L. coromandelica bark incorporated κ-carrageenan/sodium alginate hydrogel for rapid hemostasis

Hemorrhage stands out as a leading factor contributing to fatalities in trauma cases. Hemorrhage is associated with the process of hemostasis. Hemostasis is the primary stage of wound healing. Hydrogels can aid in hemostasis and minimize the duration of wound healing. Calcium is one of the clotting factors and is a key component for the activation of the coagulation cascade. In this work, we have developed a polymeric hydrogel matrix made up of κ-carrageenan and sodium alginate containing a calcium silica nanocomposite and a natural drug, namely the bark of L. coromandelica. The nanocomposite was characterized using various modalities such as XRD, FTIR, FESEM and HRTEM. The rheological and morphological properties of the pure and composite hydrogels were examined. The antimicrobial activity, hemocompatibility and hemostatic efficacy of the materials were studied using various in vitro assays including bacterial growth curve analysis, colony counting, anti-biofilm assay, hemolysis assay and in vivo clotting studies. The drug incorporated nanocomposite hydrogel exhibited superior activity in animal models.

Comparison of a minimal invasive technique micro-marsupialization versus surgical excision for mucocele of lower lip.

Objective: To compare the efficacy of micro-marsupialization with surgical excision for the treatment of mucoceles originating from the minor salivary glands of the lower lip. Our primary endpoints were the mean procedural time and the duration of post-operative healing. Study Design: Randomized Controlled Trial (RCT). Setting: Department of Oral and Maxillofacial Surgery, Faisalabad Medical University, Faisalabad. Period: 1st July 2022 to 31th Dec 2022. Material &amp; Methods: We enrolled a total of 60 patients, with 30 in each group (Group X: micro-marsupialization, Group Y: surgical excision). Random allocation was performed using a lottery method. Procedural time (in minutes) and post-operative healing duration (in days) were recorded for both groups, with follow-up assessments conducted over a 3-month period. Results: The study comprised 60 patients (40 males and 20 females) with a mean age of 34.60±10.15 years in Group X and 36.03±9.25 years in Group Y. In Group X, the mean surgical time was 6.23±0.935 minutes, while in Group Y, it was 34.70±2.493 minutes. A statistically significant difference in surgical time was observed between the two groups (p 0.0001). Regarding post-operative healing, Group X had a mean duration of 4.67±0.844 days, while Group Y had a mean duration of 6.57±0.504 days. A statistically significant difference was also noted in post-operative healing duration between the two groups (p 0.008). Conclusion: This study underscores the superiority of micro-marsupialization over surgical excision as a treatment option for mucoceles originating from the minor salivary glands of the lower lip. Micro-marsupialization offers several advantages, including significantly shorter procedural times and faster post-operative healing periods.

Bisphosphonates and atypical femoral fractures: Is the location of the fracture associated with the time of consolidation?

IntroductionBisphosphonates are commonly prescribed to mitigate the effects of osteoporosis, a condition associated with an increased risk of fracture.However, despite their success, there has been growing concern regarding the long-term use of bisphosphonates because of the increased risk of atypical subtrochanteric fractures of the femur (AFFs).The objective of this study was to describe a cohort of patients with AFFs and evaluate the risk of evolving to non-union, the time of consolidation associated with the location of the fracture, the time of use of the drug, and the implant involved in its treatment. MethodsThis retrospective study recruited patients between June 2008 and May 2018. We identified patients with AFFs according to the task force criteria and a history of bisphosphonate use.We included all patients older than 65 years, with long-term use of bisphosphonates, AFF criteria according to the task force, and a follow-up of 12 months or longer.We studied the following variables: the time of use of bisphosphonates, localization of the fracture according to Hyodo et al., implant use, and fracture healing time, considering the presence of bone callus observed in two radiologic projections, which were measured in weeks. ResultsBetween June 2008 and May 2018, 72 patients met the task force criteria for AFFs.A total of 67 patients were included in this study. From this total, 37 were in the proximal region of the femur and 31 corresponded to the diaphyseal region.Univariate and multivariate analyses showed statistical significance in the time of bone healing compared with the different locations of the fracture along the femur. ConclusionAccording to our study, the localization of AFFs in patients with a history of bisphosphonate use influences the duration of bone healing.