Dual Line Research Articles

Сравнително проучване на морфологичните качества и люпимостта на яйцата от чисти линии и кръстоски от общоползвателно и яйценосно направление

During the recent decades, the scientific community turned its attention towards the dual purpose lines and crosses that might offer a suitable solution for two problems in poultry breeding - the use of male chickens for meat and the use of female chickens for rearing in alternative types of systems for egg production. Hence, a study with four groups of birds: a dual purpose cross „Salmon“, a dual purpose purebred line „BB“, a laying line „L“ and a laying hybrid line „ISA-Brown“ was conducted. The aim was to compare the morphological traits, as well as the fertility and the hatchability of the eggs, obtained from „ISA-Brown“, compared to the dual purpose „Salmon“ reared at IAS-Kostinbrod, and the local dual purpose and laying lines involved in its creation. As a result of the conducted research, we can conclude that the selection for high performance, larger eggs and the presence of heterosis in hybrids has to some extent a positive influence on most of the morphological traits determining the quality of the eggs produced. In addition, negative consequences for their hatchability, embryo survival and some morphological parameters such as increased width of the egg and increased diameter of the yolk were also observed. On the other hand, in the stock hybrids, compared to the purebred lines and the dual purpose crosses, this can also be considered as a sign of deterioration of the nutritional quality of the eggs.

Joint optimal PMU and DULR placement in distribution network considering fault reconstruction and state estimation accuracy

Due to the rapid development of the distribution network, there is an urgent need for high-precision state estimation and safe and stable operation, this paper proposes a joint optimal placement method for Phasor Measurement Unit (PMU) and Dual Use Line Relay (DULR) in distribution networks considering fault reconstruction and state estimation accuracy. The proposed method aims to minimize the cost of PMU and DULR configuration. Based on the known pseudo-measurements and Supervisory Control and Data Acquisition (SCADA) measurements, we use the E-optimal standard design of the state estimation error covariance matrix to form the state estimation accuracy index and take the relay protection function of Feeder Terminal Unit (FTU) device and DULR device into consideration of the fault reconstruction. By solving the mixed integer semidefinite programming (MISDP) model, the optimal placement result is obtained, so that the network under normal condition and the network after multiple fault reconstruction can meet the constraints of state estimation accuracy and load loss rate respectively. The proposed method is tested on IEEE 33-node, 123-node systems as an example. The experimental results show that the proposed method is effective and practical.Note to Practitioners—This study is stimulated by the need for optimizing the placement of Micro-PMU (µPMU) in distribution network. Since the new µPMU device DULR has the same relay protection function as FTU, and distributed generation can supply power to the island after failure, this paper adds the load loss rate to the constraint conditions to study the influence of fault reconstruction on the placement scheme. A new two-step SE method considering mixed measurements was used in this paper to improve the computational efficiency of real-time SE of large-scale system while ensuring the accuracy of the calculation results. We have conducted tests in IEEE-33 and IEEE-123 systems. After preliminary verification, compared with the single fault scenario, we find that the placement scheme considering multiple fault scenarios can effectively improve the accuracy of state estimation and reduce the load loss rate of each fault scenario and only increase the cost a little. The increase in the number of DG and contact lines in the network will also effectively improve the feasibility of the placement scheme. The increase of the number of FTU configurations can effectively reduce the configuration of DULR by adding a small amount of µPMU, reduce the total cost as a result. In future studies, we will further consider the integrated configuration of µPMU, DULR and FTU, to meet more failure scenarios while reducing costs.

Modeling X chromosome inactivation using t5iLA naive human pluripotent stem cells

BackgroundX chromosome inactivation (XCI) is a critical epigenetic event for dosage compensation of X-linked genes in female mammals, ensuring developmental stability. A robust in vitro model is required for mimicking XCI during the early stages of embryonic development. This methodology article introduces an advanced framework for the in-depth study of XCI using human pluripotent stem cells (hPSCs). By focusing on the transition between naive and primed pluripotent states, we highlight the role of long non-coding RNA X-inactive specific transcript (XIST) and epigenetic alterations in mediating XCI.ResultsOur methodology enables the distinction between naive and primed hESCs based on XIST expression and the activity of X-linked reporters, facilitating the investigation of XCI initiation and maintenance. Through detailed experimental procedures, we demonstrate the utility of our hESC lines in modeling the process of human XCI, including the establishment of conditions for random XCI induction and the analysis of X chromosome reactivation.MethodsThe study outlines a comprehensive approach for characterizing the X chromosome status in hPSCs, employing dual fluorescent reporter hESC lines. These reporter lines enable real-time tracking of XCI dynamics through differentiation processes. We detailed protocols for the induction of X chromosome reactivation and inactivation, as well as the X status characterization methods including cultivation of hESCs, flow cytometric analysis, RNA fluorescence in situ hybridization (FISH), and transcriptome sequencing, providing a step-by-step guide for researchers to investigate XCI mechanisms in vitro.ConclusionsThis article provides a detailed, reproducible methodology for studying XCI mechanisms in vitro, employing hPSCs as a model system. It presents a significant advance in our ability to investigate XCI, offering potential applications in developmental biology, disease modeling, and regenerative medicine. By facilitating the study of XCI dynamics, this methodological framework paves the way for deeper understanding and manipulation of this fundamental biological process.

Temporal-spatial and energy dissipation characteristics of vortex evolutions in Francis turbine during load reduction

This study numerically investigates the load reduction effects, on a model Francis turbine, combining cavitation model and structured dynamic grid technique. The results indicate that the vapor volume in the draft tube undergoes two rapid increases and decreases until cavitation ceases. The precessing vortex rope transitions from a strong helical structure to axial contraction as ellipticity increases, ultimately forming a discrete band before disappearing. Initially, vapor volume in the runner increases gradually and linearly, followed by continued growth with a consistent pulsation amplitude. The inter-blade vortex (IBV) first appears at the blade trailing edge and then develops into a complete structure extending from the runner crown to the blade trailing edge, driven by pulsating vapor volume growth. Axial force extracted by the runner changes significantly and correlates closely with variations in the vapor volume in the runner. Flow separation in the runner occurs near the runner crown, forming dual separation lines that enhance IBV formation, which highlights the significant influence of crown-proximal flow separation on IBV development. Regarding energy loss, initial decreases followed by increases are observed in both the draft tube and runner, with draft tube losses consistently exceeding 57.4% and runner losses exceeding 27.1%. Turbulent kinetic energy generation and Reynolds stress are the primary forms of energy dissipation, with high-value regions corresponding to vortex locations, underscoring the substantial role of vortices in energy dissipation. This study provides new insights into the evolution of vortices and energy dissipation characteristics during load reduction in Francis turbines.

Thin Ga-doped ZnO Film with Enhanced Dual Visible Lines Emission

Introduction: In this study, a simple and facile route was employed to prepare a highly transparent and luminescent ultra-thin gallium doped ZnO film (GZO). Methods: The thin GZO film has been deposited using the simultaneously ultrasonic vibration and sol-gel spin-spray coating technique. The structural and optical properties of pure and doped thin films were investigated by various methods, such as X-ray diffraction (XRD), X-ray photoemission spectroscopy (XPS), scanning electron microscopy (SEM), UV-Vis, and PL spectroscopy. Results: XRD results indicated that both pure and doped ZnO films had a hexagonal wurtzite structure with (101) preferred orientation. XPS and EDX studies confirmed the incorporation and presence of Ga ions into the ZnO lattice structure. The doped sample showed nearly 90% of transparency, and a strong blue-green emission in the visible region. Conclusion: The obtained results proved that the prepared thin film could be a novel candidate for optoelectronic applications.

Design of dual port 9T SRAM cell with parallel processing and high performance computing

Abstract To meet industry requirements of higher transistor count SRAM cells this paper is proposing, a nine-transistor configuration static random access memory (SRAM) cell which is accessible by dual bit line and performing simultaneous read and write operations. The suggested 9T (P9T PS) cell is constructed at 32 nm CMOS and is operating at an operating voltage 0.9 V. To justify the refined performance of proposed design various analysis are carried out that shows the static noise margins (SNM) that occurs while executing the read operation is 0.368 V and hold operation by the cell is 0.364 V, while the write operation has an SNM of 0.422 V. The access time for write and read functions of the cell is found to be 1.2 nS and 4.5 nS respectively. The P9T PS SRAM is compared with other dual-port and single-port 9T SRAM cells is stand out to be pre-eminence in performance in terms of noise immunity, temperature variations, power consumption (static and dynamic both), power delay product (PDP), SNM to PDP and area ratio (SAPR) and electric quality matric. Total leakage power for P9T PS cell is found to be 22.1 nW. The P9T PS bit cell is also found to be power efficient as it is consuming minimal power for all of the operations. Area layout of the P9T (PS) is found to be 22.372 μm2. The results are also supported by the process corner variation analysis for four different corners. LT-spice software is used to carry out all the simulations and analysis.

Dual Dirac Nodal Line in Nearly Freestanding Electronic Structure of β-Sn Monolayer.

Two-dimensional topological insulators (2D TIs) have distinct electronic properties that make them attractive for various applications, especially in spintronics. The conductive edge states in 2D TIs are protected from disorder and perturbations and are spin-polarized, which restrict current flow to a single spin orientation. In contrast, topological nodal line semimetals (TNLSM) are distinct from TIs because of the presence of a 1D ring of degeneracy formed from two bands that cross each other along a line in the Brillouin zone. These nodal lines are protected by topology and can be destroyed only by breaking certain symmetry conditions, making them highly resilient to disorder and defects. However, 2D TNLSMs do not possess protected boundary modes, which makes their investigation challenging. There have been several theoretical predictions of 2D TNLSMs, however, experimental realizations are rare. β-Sn, a metallic allotrope of tin with a superconducting temperature of 3.72 K, may be a candidate for a topological superconductor that can host Majorana Fermions for quantum computing. In this work, single layers of α-Sn and β-Sn on a Cu(111) substrate are successfully prepared and studied using scanning tunneling microscopy, angle-resolved photoemission spectroscopy, and density functional theory calculations. The lattice and electronic structure undergo a topological transition from 2D topological insulator α-Sn to 2D TNLSM β-Sn, with two types of nodal lines coexisting in monolayer β-Sn. Such a realization of two types of nodal lines in one 2D material has not been reported to date. Moreover, we also observed an unexpected phenomenon of freestanding-like electronic structures of β-Sn/Cu(111), highlighting the potential of ultrathin β-Sn films as a platform for exploring the electronic properties of 2D TNLSM and topological superconductors, such as few-layer superconducting β-Sn in lateral contact with topological nodal line single-layer β-Sn.

Human induced pluripotent stem cells for live cell cycle monitoring and endogenous gene activation

The fluorescence ubiquitination cell cycle inhibitor (FUCCI) has been introduced to monitor cell cycle activity in living cells, including human induced pluripotent stem cells (hiPSC) and derived cell types. We have recently developed hiPSC with stable expression of dCas9VPR for endogenous gene activation and a Citrine-tagged ACTN2 cell line to monitor sarcomere development and function in muscle cells. Here, we present dual and triple transgenic hiPSC lines developed by genomic integration of FUCCI with and without dCas9VPR into the ROSA26 and AAVS1 loci, respectively, in the previously introduced ACTN2-Citrine line. Functionality of the transgenes was demonstrated in the novel hiPSC line, which we introduce as Myo-CCER and CraCCER.

A genome-wide CRISPR/Cas9 screen identifies calreticulin as a selective repressor of ATF6α.

Activating transcription factor 6 (ATF6) is one of three endoplasmic reticulum (ER) transmembrane stress sensors that mediate the unfolded protein response (UPR). Despite its crucial role in long-term ER stress adaptation, regulation of ATF6 alpha (α) signalling remains poorly understood, possibly because its activation involves ER-to-Golgi and nuclear trafficking. Here, we generated an ATF6α/Inositol-requiring kinase 1 (IRE1) dual UPR reporter CHO-K1 cell line and performed an unbiased genome-wide CRISPR/Cas9 mutagenesis screen to systematically profile genetic factors that specifically contribute to ATF6α signalling in the presence and absence of ER stress. The screen identified both anticipated and new candidate genes that regulate ATF6α activation. Among these, calreticulin (CRT), a key ER luminal chaperone, selectively repressed ATF6α signalling: Cells lacking CRT constitutively activated a BiP::sfGFP ATF6α-dependent reporter, had higher BiP levels and an increased rate of trafficking and processing of ATF6α. Purified CRT interacted with the luminal domain of ATF6α in vitro and the two proteins co-immunoprecipitated from cell lysates. CRT depletion exposed a negative feedback loop implicating ATF6α in repressing IRE1 activity basally and overexpression of CRT reversed this repression. Our findings indicate that CRT, beyond its known role as a chaperone, also serves as an ER repressor of ATF6α to selectively regulate one arm of the UPR.

Enhancing ultra low temperature district heating systems with neighborhood-scale heat pump and triple-pipe distribution: A techno-economic analysis

Ultra-Low Temperature District Heating (ULTDH) systems are advanced District Heating (DH) systems enabling the efficient utilization of low-temperature heat sources for heating systems. A significant challenge in ULTDH systems is the economic inefficiency associated with use of booster Heat Pump (HP) in buildings to achieve the desired Domestic Hot Water (DHW) temperature. To address this issue, this study proposes an innovative ULTDH system incorporating neighborhood-scale water-source booster HPs and distribution triple-pipes. Triple pipe's dual supply line capabilities facilitate the neighborhood-scale water-source booster HPs to be brought out at the neighborhood scale, demonstrating an intelligent approach. This approach reduces the capital investment and enhances the Coefficient of Performance (COP) by utilizing supply line of DH system as the heat source. A comprehensive case study is conducted to demonstrate the superior techno-economic performance of the proposed ULTDH system compared to Low Temperature District Heating (LTDH) as the benchmark solution. All components of both systems are sized by determining the DHW and Space Heating (SH) demands for each neighborhood. Thermodynamic analysis is conducted at a 1-min time resolution for an entire year. Six different heat production scenarios are developed to determine the Levelized Cost of Heat (LCOH). The results indicate that while the energy performance of the ULTDH surpasses LTDH, the rise in electricity prices favors the ULTDH system.

Mutual inductance parameter measurement and experimental research of double circuit based on different frequency method

AbstractIn order to improve the measurement accuracy of mutual inductance parameters of transmission lines in the environment of double circuit power lines without power outage, this paper establishes simulation models for measuring mutual inductance parameters of transmission lines using the different frequency method in two modes: equal and unequal zero sequence self‐parameters of two circuit lines, using a parallel zero sequence coupling model of double circuit power lines. In the article, simulation analysis is conducted on the line parameters with a coupling coefficient between 0.4 and 0.6 and a line length of 20–50 km. In order to further verify the correctness of the simulation model and measurement methods, a test bench was established based on the principle of line mutual inductance parameter testing in a laboratory dual circuit line without a power outage environment for experimental testing. By comparing the test values under experimental conditions with the standard values, it has been proven that the model and method can meet the actual measurement requirements of engineering.

Pyrite-stimulated bio-reductive immobilization of perrhenate: Insights from integrated biotic and abiotic perspectives

Microbes possessing electron transfer capabilities hold great promise for remediating subsurface contaminated by redox-active radionuclides such as technetium-99 (99TcO4−) through bio-transformation of soluble contaminants into their sparingly soluble forms. However, the practical application of this concept has been impeded due to the low electron transfer efficiency and long-term product stability under various biogeochemical conditions. Herein, we proposed and tested a pyrite-stimulated bio-immobilization strategy for immobilizing ReO4− (a nonradioactive analogue of 99TcO4−) using sulfate-reducing bacteria (SRB), with a focus on pure-cultured Desulfovibrio vulgaris. Pyrite acted as an effective stimulant for the bio-transformation of ReO4−, boosting the removal rate of ReO4− (50 mg/L) in a solution from 2.8 % (without pyrite) to 100 %. Moreover, the immobilized products showed almost no signs of remobilization during 168 days of monitoring. Dual lines of evidence were presented to elucidate the underlying mechanisms for the pyrite-enhanced bio-activity. Transcriptomic analysis revealed a global upregulation of genes associated with electron conductive cytochromes c network, extracellular tryptophan, and intracellular electron transfer units, leading to enhanced ReO4− bio-reduction. Spectroscopic analysis confirmed the long-term stability of the bio-immobilized products, wherein ReO4− is reduced to stable Re(IV) oxides and Re(IV) sulfides. This work provides a novel green strategy for remediation of radionuclides- or heavy metals-contaminated sites.

AN EXPERIMENTAL INVESTIGATION OF THE MECHANICAL PROPERTIES OF FUSED FILAMENT FABRICATED NYLON-CARBON FIBER COMPOSITES

Additive Manufacturing (AM) is a rapidly growing field in both the researching and the industrial world, as it produces highly customized and geometrically complex objects. The most well-known AM technology for plastics is Fused Filament Fabrication (FFF), in which a thermoplastic filament is melted and extruded through a nozzle on the printing bed. A wide variety of printing parameters affect the quality of the printed objects, such as printing speed, infill density, infill pattern, build orientation, layer height, etc. In literature, there is already extended research of the impact of the printing parameters on the mechanical properties of the most common thermoplastics, such as ABS and PETG. However, the development of advanced thermoplastic materials, such as Nylon composites reinforced with carbon fibers (Nylon-CF), requires a further investigation of the effect of the printing parameters on those advanced composites. In the current study, an in-depth correlation of all the major printing parameters (infill pattern, infill density, dual line infill and printing speed) with all the major mechanical properties (tensile strength, compressive strength and bending strength) of Nylon-CF is carried out.

An improved problem transformation algorithm for large-scale multi-objective optimization

Solving Large-Scale Multi-Objective Optimization Problems (LSMOPs) is the major challenge in evolution computation. Due to the large number of decision variables involved, it is not easy for existing multi-objective evolutionary algorithms to search the entire decision variable space with limited computation resources. To address the above problem, a large-scale multi-objective evolutionary algorithm based on problem transformation, called LSMOEA/PT, is presented in this paper. LSMOEA/PT uses an improved problem transformation strategy to transform LSMOPs into small-scale multi-objective problems to accelerate convergence speed and reduce computational resources. Specifically, The transformation strategy selects reference solutions to initialize a weight population by applying the opposite individual method. After optimizing the weight population, a dual line strategy is implemented to combine the weight vectors with the decision variables from the original population, thereby generating a new population. The new population is combined with the original population for environment selection. In LSMOEA/PT, a double learning swarm optimizer is introduced to speed up the convergence of the population. A uniform distribution strategy is conducted to update transformed solutions after the problem transformation process, increasing the diversity of the population. Experimental results show that LSMOEA/PT is competitive with eleven state-of-the-art algorithms on large-scale multi-objective optimization test problems with up to 2000 decision variables.

ISuRe-HadCre is an essential tool for effective conditional genetics.

Methods for modifying gene function at high spatiotemporal resolution in mice have revolutionized biomedical research, with Cre-loxP being the most widely used technology. However, the Cre-loxP technology has several drawbacks, including weak activity, leakiness, toxicity, and low reliability of existing Cre-reporters. This is mainly because different genes flanked by loxP sites (floxed) vary widely in their sensitivity to Cre-mediated recombination. Here, we report the generation, validation, and utility of iSuRe-HadCre, a new dual Cre-reporter and deleter mouse line that avoids these drawbacks. iSuRe-HadCre achieves this through a novel inducible dual-recombinase genetic cascade that ensures that cells expressing a fluorescent reporter had only transient Cre activity, that is nonetheless sufficient to effectively delete floxed genes. iSuRe-HadCre worked reliably in all cell types and for the 13 floxed genes tested. This new tool will enable the precise, efficient, and trustworthy analysis of gene function in entire mouse tissues or in single cells.

SAW Humidity Sensing with rr-P3HT Polymer Films.

In the present paper the humidity sensing properties of regioregular rr-P3HT (poly-3-hexylthiophene) polymer films is investigated by means of surface acoustic wave (SAW) based sensors implemented on LiNbO3 (1280 Y-X) and ST-quartz piezoelectric substrates. The polymeric layers were deposited along the SAW propagation path by spray coating method and the layers thickness was measured by atomic force microscopy (AFM) technique. The response of the SAW devices to relative humidity (rh) changes in the range ~5-60% has been investigated by measuring the SAW phase and frequency changes induced by the (rh) absorption in the rr-P3HT layer. The SAW sensor implemented onto LiNbO3 showed improved performance as the thickness of the membrane increases (from 40 to 240 nm): for 240 nm thick polymeric membrane a phase shift of about -1.2 deg and -8.2 deg was measured for the fundamental (~78 MHz operating frequency) and 3rd (~234 MHz) harmonic wave at (rh) = 60%. A thick rr-P3HT film (~600 nm) was deposited onto the quartz-based SAW sensor: the sensor showed a linear frequency shift of ~-20.5 Hz per unit (rh) changes in the ~5-~50% rh range, and a quite fast response (~5 s) even at low humidity level (~5% rh). The LiNbO3 and quartz-based sensors response was assessed by using a dual delay line system to reduce unwanted common mode signals. The simple and cheap spray coating technology for the rr-P3HT polymer films deposition, complemented with fast low level humidity detection of the tested SAW sensors (much faster than the commercially available Michell SF-52 device), highlight their potential in a low-medium range humidity sensing application.

P‐77: A Novel Frequency Doubling Technology with Adaptive Dither

In this paper, in order to improve the refresh rate of large‐size LCD displays, a new optimized driving scheme combined with new GOA timing is implemented in hardware platform, which is called adaptive scanning for frequency multiplication (ASF) technology, and it can be applied to TV, MNT, NB and other fields. Firstly, this paper introduces the basic principle of Dual Line Gate technology (DLG), which is widely used, and analyzes its advantages and disadvantages. And then ASF technology is proposed to solve the disadvantages of DLG technology. The new optimized driving scheme mainly applied inter‐frame adaptive dither algorithm(ADA), odd and even frame interlaced compensation algorithm(OEC) and application mode for intelligent detection of pictures. A prototype driving circuit was fabricated, and various detection tests have been implemented. The results show the refresh rate is doubled without increasing hardware resources, and this technology avoids the loss of image quality.

SPOC 双线融合教学设计与实践

Online teaching platforms and technologies have become important means of offline teaching. The two teaching scenarios should be fully integrated and collaborate to complete teaching tasks. Following the concept of collaborative teaching and the integration of teaching elements by Josh Bersin, this paper reconstructs and designs course teaching content through four stages: learner demand feature recognition, learning plan formulation, learning resource development, and learning plan execution. Three integration methods, including flipping, flexibility, and complementary classrooms, are introduced to complete teaching practice. The assessment results of final close-book paper test and the satisfaction evaluation of integrated teaching give affirmation to the teaching effectiveness. Therefore, it can be considered that teachers should choose different dual line integrated teaching models based on the differences in course content to drive learning pace and improve classroom teaching efficiency.

Development of a Front End Array for Broadband Phased Array Receiver

The receiver is a signal receiving device placed at the focus of the telescope. In order to improve the observation efficiency, the concept of phased array receiver has been proposed in recent years, which places a small phased array at the focal plane of the reflector, and flexible pattern and beam scanning functions can be achieved through a beamforming network. If combined with the element multiplexing, all beams within the entire field of view can be observed simultaneously to achieve continuous sky coverage. This article focuses on the front-end array of phased array receiver at 0.7–1.8 GHz for QiTai Telescope, and designs a Vivaldi antenna array of PCB structure with dual line polarization. Each polarization antenna is designed to arrange in a rectangle manner by 11 × 10. Based on the simulation results of the focal field, 32, 18, and eight elements were selected to form one beam at 0.7, 1.25, and 1.8 GHz. An analog beamforming network was constructed, and the measured gains of axial beam under uniform weighting were 19.32, 13.72, and 15.22 dBi. Combining the beam scanning method of reflector antenna, the pattern test of different position element sets required for PAF beam scanning was carried out under independent array. The pattern optimization at 1.25 GHz was carried out by weighting method of conjugate field matching. Compared with uniform weighting, the gain, sidelobe level, and main beam direction under conjugate field matching have been improved. Although the above test and simulation results are slightly different, which is related to the passive array and laboratory testing condition, the relevant work has accumulated experience in the development of the front-end array for the phased array receiver, and has good guiding significance for future performance verification after the array is installed on the telescope.

An intranasal combination vaccine induces systemic and mucosal immunity against COVID-19 and influenza

Despite prolonged surveillance and interventions, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and influenza viruses continue to pose a severe global health burden. Thus, we developed a chimpanzee adenovirus-based combination vaccine, AdC68-HATRBD, with dual specificity against SARS-CoV-2 and influenza virus. When used as a standalone vaccine, intranasal immunization with AdC68-HATRBD induced comprehensive and potent immune responses consisting of immunoglobin (Ig) G, mucosal IgA, neutralizing antibodies, and memory T cells, which protected the mice from BA.5.2 and pandemic H1N1 infections. When used as a heterologous booster, AdC68-HATRBD markedly improved the protective immune response of the licensed SARS-CoV-2 or influenza vaccine. Therefore, whether administered intranasally as a standalone or booster vaccine, this combination vaccine is a valuable strategy to enhance the overall vaccine efficacy by inducing robust systemic and mucosal immune responses, thereby conferring dual lines of immunological defenses for these two viruses.