With the aim of evaluate the distribution of assimilates in tomato (Lycopersicon esculentum Mill), an essay under greenhouse was carried out with plants growing in hydroponic solution. The treatments were: - H1: Controls with the first leaf supplied with glucose [ C 14 (U) ]; H2: Controls with the second leaf supplied with glucose [ C 14 (U) ]; H3: Controls with the third leaf supplied with glucose [ C 14 (U) ]. In all treatments, the supplied leaf was situated among the first and the second truss. H4: Pruning of the third leaf (among the first and the second truss) supplying the second leaf. The pruning was done 25 days after the anthesis of the first cluster and next to 7 days, the leaves were supplied in all treatments. 24 hours later, the plants were fractionated in root, shoot, basal leaves, expanded leaves, non expanded leaves, 1st, 2nd and 3rd trusses and apex. The vegetal material remained in stove up to constant weight. The dry weight of the organs had a coefficient of variability of 22%. The samples were digested with OHNa 9N, homogenized, and it was added scintillating solution Bray + cab-o-sil at 5%. The activity of the samples were measured in a liquid scintillation counter Beckman LS 100 C. Mean values of five plants by treatment were used for the data computation. The pruning of the third leaf modified the pattern distribution of assimilates in the plant. The group of clusters were the most important sinks in all treatments, reaching the highest value in H4 (70%). The stem was an important sink in all treatments. The most contribution to it (30%) was done by the second leaf (H2). The removal of the third leaf increased the amount of assimilates entering the fruits and decreased the amount of glucose entering the stem.