Dry Soil Research Articles

Effect of polypropylene fibres on the shear strength of fine-grained soil

This paper presents the results of shear strength tests of fine-grained soil reinforced by randomly oriented fibres. The tests were carried out in a direct shear apparatus on samples with a diameter of 100 mm and a height of 20 mm. The samples were formed directly in the apparatus box at a natural moisture content and two values of the degree of compaction (IS = 0:79 and 0.90). The studies were carried out for samples of natural moisture content and for soaked ones. The two types of polypropylene fibres were used: monofilament and fibrillated (of traded names Fibermesh 300-e3 and SikaCem Fiber-12, respectively). The fibre content was 0.25; 0.50 and 1.00% by the weight of the dry soil. The results showed that the presence of fibre within the soil increased its the shear strength. The improvement of the shear strength was related to the type of reinforcement, its content and the soil parameters. The maximum increase in shear strength was 47% compared to the shear strength of the unreinforced soil. The increase in shear strength values were related mainly to the increase in the angle of internal friction of the soil. It was found that as the degree of compaction of the soil increases, the higher enhance of the shear strength of reinforced soil occurs. It was also found that the improvement of shear strength of reinforced soaked samples was more significant than for un-soaked ones.

The effects of earthworm species on organic matter transformations and soil microbial communities are only partially related to their bioturbation activity

Earthworms are pivotal in shaping soil ecological processes through their bioturbation activity and organic matter consumption. Earthworm species are known to have different impacts on soil structure, but only a small number of species have been studied so far, and few studies have examined how earthworms simultaneously affect soil functions. Here, we measured the impact of different earthworm species on soil structure (bioturbation function), carbon (C) and nitrogen (N) dynamics and the microbial community (organic matter transformation function), while exploring the links between these functions, across distinct soil compartments (surface casts, below-ground drilosphere, and bulk soil). Six earthworm species (Lumbricus terrestris, Allolobophora chlorotica, Octolasion cyaneum, Octodrilus complanatus, Aporrectodea caliginosa meridionalis and Microscolex dubius) of different ecological categories and functional groups were incubated in soil cores with soil and alfalfa litter for 6 weeks. Our results on the bioturbation function illustrated a great diversity of behaviors and confirmed the relevance of a functional classification based on bioturbation metrics. The main microbial hotspots were surface casts, whose characteristics allowed to distinguish two groups of species. Octod. complanatus, L. terrestris and M. dubius induced high humidity (respectively, +57, +48, +74%), high C (respectively, 19.9, 24.8, 33.2 g kg⁻1 dry soil) and N (respectively, 2, 2.3, 3.2 g kg⁻1 dry soil) content and microbial community selection, promoting C and N mineralization. The three other species had a lower impact. The below-ground drilosphere only showed specific characteristics in the case of L. terrestris. The effects of the studied species on the organic matter transformation function did not align with their bioturbation activities nor with their ecological category. These findings show that the contribution of earthworms to C and N turnover is only partially dependent on their bioturbation effects and suggest the usefulness of developing distinct functional groups based on the specific soil functions under consideration.

Dredged Canals, Wetland Loss, and Legacy

AbstractThe direct effects of converting coastal wetlands to open water by dredging them can be magnified by indirect effects. For example, dredged canals allow for recovery of mineral fluids 1000 s of m belowground which may induce geological subsidence or faulting; the dredged material deposited at the surface creates levees that redirect overland water flows. These indirect factors may stress wetland plants enough so that additional wetland habitat is converted to open water as a result of longer intervals of wetland soil waterlogging and drying, sulfide toxicity, less organic matter and sediment accumulation, and greater erosion. We quantified the indirect effects by demonstrating a robust dose–response relationship between coastal land loss and canal density in the Mississippi and Niger river deltas over 5 decades. Importantly, the ratio of land loss to canal area increases with time—a legacy effect. Surface impediments to water movements rather than belowground subsidence are the dominant causal factor. We also found that flood protection levees on the main river channel did not significantly magnify the effect of dredging on wetland loss. The cumulative effect of these direct and indirect consequences in coastal Louisiana is enormous and continuing, equaling many tens of billions dollars annually. Understanding these effects supports the rejection of a hypothesis that regional river channel flood protection levees or fluid withdrawal is of greater importance than the local changes in wetland hydrology. Wetland restoration/mitigation of dredging impacts on these two coasts can be implemented at a relatively low cost and quickly if this paradigm of the causes of coastal wetland losses is adopted.

Disentangling the Effects of Atmospheric and Soil Dryness on Autumn Phenology across the Northern Hemisphere

In recent decades, drought has intensified along with continuous global warming, significantly impacting terrestrial vegetation. High atmospheric water demand, indicated by vapor pressure deficit (VPD), and insufficient soil moisture (SM) are considered the primary factors causing drought stress in vegetation. However, the influences of VPD and SM on the autumn phenology are still unknown. Using satellite observations and meteorological data, we examined the impacts of VPD and SM on the end of the growing season (EOS) across the Northern Hemisphere (>30°N) from 1982 to 2022. We found that VPD and SM were as important as temperature, precipitation, and radiation in controlling the variations in the EOS. Moreover, the EOS was predominantly influenced by VPD or SM in more than one-third (33.8%) of the study area. In particular, a ridge regression analysis indicated that the EOS was more sensitive to VPD than to SM and the other climatic factors, with 25% of the pixels showing the highest sensitivity to VPD. In addition, the effects of VPD and SM on the EOS varied among biome types and climate zones. VPD significantly advanced the EOS in 25.8% of temperate grasslands, while SM had the greatest impact on advancing the EOS in 17.7% of temperate coniferous forests. Additionally, 27.7% of the midlatitude steppe (BSk) exhibited a significant negative correlation between VPD and the EOS, while 19.4% of the marine west coast climate (Cfb) showed a positive correlation between SM and the EOS. We also demonstrated that the correlation between VPD and the EOS was linearly affected by VPD and the leaf area index, while the correlation between SM and the EOS was affected by SM, precipitation, and the leaf area index. Our study highlights the importance of VPD and SM in regulating autumn phenology and enhances our understanding of terrestrial ecosystem responses to climate change.

Abrupt increase in Arctic-Subarctic wildfires caused by future permafrost thaw

Unabated 21st-century climate change will accelerate Arctic-Subarctic permafrost thaw which can intensify microbial degradation of carbon-rich soils, methane emissions, and global warming. The impact of permafrost thaw on future Arctic-Subarctic wildfires and the associated release of greenhouse gases and aerosols is less well understood. Here we present a comprehensive analysis of the effect of future permafrost thaw on land surface processes in the Arctic-Subarctic region using the CESM2 large ensemble forced by the SSP3-7.0 greenhouse gas emission scenario. Analyzing 50 greenhouse warming simulations, which capture the coupling between permafrost, hydrology, and atmosphere, we find that projected rapid permafrost thaw leads to massive soil drying, surface warming, and reduction of relative humidity over the Arctic-Subarctic region. These combined processes lead to nonlinear late-21st-century regime shifts in the coupled soil-hydrology system and rapid intensification of wildfires in western Siberia and Canada.

Morphophysiological and Histopathological Effects of Ammonium Sulfate Fertilizer on Aporrectodea trapezoides (Dugès, 1828) Earthworm.

The present study used the adult earthworm Aporrectodea trapezoides as a bioindicator species to look into the possible dangers of ammonium sulfate (AS) fertilizer. Two complementary toxicity tests were conducted to determine the LC50values, growth rate inhibition, morphological alterations, and histopathological texture of worms. The lethality test included four increasing concentrations of AS fertilizer (ranging from 2500 to 7500 mg/kg of dry soil weight (d.w.)), while sub-lethal concentrations were based on 10%, 30%, 40%, and 50% of the 14-day median lethal concentration (LC50), with a control group included for both tests. The LC(50) values for AS fertilizer were significantly higher at 7 days (4831.13 mg/kg d.w.) than at 14 days (2698.67 mg/kg d.w.) of exposure. Notably, earthworms exhibited significant growth rate inhibition under exposure to various concentrations and time durations (14/28 exposure days). Morphological alterations such as clitellar swelling, bloody lesions, whole body coiling and constriction, body strangulation, and fragmentation were accentuated steadily, with higher concentrations. Histopathological manifestations included severe injuries to the circular and longitudinal muscular layers, vacuolation, muscle layer atrophy, degradation of the chloragogenous tissue in the intestine, collapsed digestive epithelium of the pharynx with weak reserve inclusion, and fibrosis of blood vessels. These effects were primarily influenced by increasing concentrations of fertilizer and time exposure. The study highlights the strong relationship between concentration and exposure time responses and underscores the potential of A. trapezoides earthworms as valuable biological control agents against acidic ammonium sulfate fertilizer. Importantly, this research contributes to the use of such biomarkers in evaluating soil toxicity and the biological control of environmental risk assessment associated with chemical fertilizers.

Yield-stress effects on spontaneous imbibition in paper-based kits

The classic Richards equation is a good model for predicting imbibition of viscous fluids in porous materials such as dry soils or filter papers. It cannot, in principle, be used for physiological fluids such as blood simply because such fluids often exhibit a variety of non-Newtonian behavior such as a yield stress. In the present work, we have theoretically extended the classic Richards equation to viscoplastic fluids obeying the Bingham model using the concept of the effective viscosity together with the bundle-of-tube model. The new imbibition model could partly resolve the discrepancy reported in the literature between the predictions of the classic Richards equation for the stain growth of sessile blood droplets in a typical filter paper. A better fit, however, requires considering other non-Newtonian effects of the blood such as its viscoelasticity. Using the Bingham-modified Richards equation, it is demonstrated that yield stress in a test fluid has a retarding effect on the imbibition phenomenon, so that such fluids may not necessarily reach the test line of a paper-based diagnostic kit. But yield stress is predicted to extend the duration of the quasi-steady regime on the test line of diagnostic kits, which is a desirable effect. The results suggest that inducing (or elevating) the level of yield stress in a test liquid such as blood can be used as a passive means to control imbibition characteristics in paper-based systems.

Warming suppresses grassland recovery in biomass but not in community composition after grazing exclusion in a Mongolian grassland

We conducted a 4-year temperature manipulation experiment in a Mongolian grassland to examine the effect of daytime and nighttime warming on grassland recovery after grazing exclusion. After constructing a livestock exclusion fence in the grassland, we established daytime and daytime-and-nighttime warming treatments within the fenced area by a combination of open-top chambers (OTC) and electric heaters. We measured the numbers of plants and aboveground biomass by species after recording percentage vegetation cover every summer for three warming treatments inside the fence—non-warming, daytime warming, and daytime-and-nighttime warming—and for the grassland outside of the fence. OTCs increased daytime temperature by about 2.0 °C, and heaters increased nighttime temperature by 0.9 °C during the growing period. Grazing exclusion had little effect on grassland biomass but reduced the abundance of poorly palatable species and modified plant community composition. Daytime warming decreased soil moisture and lowered aboveground biomass within the fenced grassland but had little effect on plant community composition. Nighttime warming lowered soil moisture further but its effects on grassland biomass and community composition were undetectable. We concluded that recovery of plant biomass in grasslands degraded by grazing would be lowered by future climate warming through soil drying. Because warming had little effect on the recovery of community composition, adverse effects of warming on grassland recovery might be offset by improving plant productivity through mitigation of soil drying by watering. Soil drying due to nighttime warming might have detectable effects on vegetation when warming persists for a long time.

Microplastics in the Soils of the Thala Hills, East Antarctica

For the first time, on an example of the Vecherny Oasis, Thala Hills, Enderby Land, data on the content of microplastic particles (less than 5 mm) in the soils of East Antarctica were obtained. Seven samples taken from a depth of 0–15 cm were analyzed. Two soil fractions (less than 1 mm and 1–5 mm) were studied in 3 replicates (42 individual samples). The technique for isolating microplastic particles included soil sieving, density separation in zinc chloride solution, centrifugation, vacuum filtration, and microscopic analysis. For filtration, glass fiber filters with a pore diameter of 1.6 μm were used. Quantification of microplastic particles was carried out using a microscope, digital camera and advisory software. It was found that microplastic particles present in all analyzed samples. Their number varies from 66 to 1933 units/kg of dry soil. In most cases, particles less than 1 mm predominate, accounting from 70 to 100%. In 70% of cases, fibers dominate, in 30% – fragments of irregularly shaped plastics; films occur singly. There is no clearly defined confinement of increased amount of microplastic particles to infrastructure facilities; this may be a consequence of the influence of other factors, including local and long-range transport.

Drought impairs detritivore feeding activity more strongly in northern than in southern European latitudes

Soil detritivores play key roles in decomposition processes closely related to ecosystem services. Drought and soil organic carbon depletion due to agricultural management are detrimental to soil biodiversity, but their interactive effects on soil biota and associated processes have not been thoroughly investigated. In 2018, we used rain-out shelters to experimentally induce drought in wheat fields of contrasting levels of organic carbon in Sweden, Germany and Spain. That year Europe experienced a climatic dipole, with exceptionally severe droughts in northern latitudes. We assessed the feeding activity of soil detritivores by bait-lamina tests, and measured several abiotic and biotic soil parameters. In the peak of the dipole drought (summer) southern fields had the driest soils. Nonetheless, detritivore feeding activity responded to the experimental drought by shifting to deeper soil layers there. Low soil organic carbon (SOC) levels exacerbated the latter effect. However, in this same period, feeding activity in northern and central Europe was two orders of magnitude lower than in the south, and failed to respond to either the experimental treatment and/or SOC levels. Using mixed-effects longitudinal random forests, we detected various candidate drivers of detritivore feeding activity: soil moisture, phosphorus content, bacteria and nematodes. Different bacterial taxa were associated to detritivory in each country, but their potential influence was pervasive. Thus, our results suggest that drought had adverse effects on detritivore feeding, which were exacerbated northwards due to the climatic dipole. Increased SOC levels mitigated drought effects only in southern soils. Regional adaptation of soil biota to aridity could explain the response of detritivores in southern Europe to drought. Machine learning algorithms arise as useful tools for exploring potential drivers relating biodiversity to soil processes. Overall, future research on the effects of drought on soil biodiversity and processes will be key for tackling climate change impacts in terrestrial ecosystems.

Laboratory-scale thermo-activated piles under long continuous operation and different mobilised shaft resistance

AbstractThis paper examines the shaft resistance mobilisation ratio as a predictor of cumulative displacement of small-scale floating and end-bearing energy pile foundations subjected to vertical compressive loads embedded in dry sandy soils. A reduced friction model pile was subjected to different mechanical loads and two long-duration, cyclic heating/recovery temperature changes. The pile, soil and container temperatures, pile strains, and vertical displacements are monitored, analysed, and discussed. The results further validate numerical analyses that propose the shaft resistance mobilisation ratio as a variable to identify thresholds above which permanent cyclic thermo-induced deformations may occur. Overall, the experimentally observed responses indicate incremental deformations as the shaft resistance mobilisation ratio increased. The results also suggest that a mobilisation ratio of 66% could be a potential conservative lower-bound limit that could control the increment of thermal-induced vertical displacements in the long term under free pile head conditions. This suggests that a performance-based design would be a reasonable approach for energy piles, and monitoring programs should be set in the field before loading and thermo-activation.

Does Nitrogen Fertilization Improve Nitrogen-Use Efficiency in Spring Wheat?

To investigate the effects and mechanism of prolonged inorganic nitrogen (N) fertilization on the N-use efficiency of spring wheat (Triticum aestivum L.), a long-term study initiated in 2003 was conducted. The study analyzed how N fertilization affects dry matter translocation, N translocation, soil NO3-N, and N-use efficiency. Five different N-fertilizer rate treatments were tested: N0, N52.5, N105, N157.5, and N210, corresponding to annual N fertilizer doses of 0, 52.5, 105.0, 157.5, and 210.0 kg N ha−1, respectively. Results showed that increasing N-fertilizer rates significantly enhanced the two-year average dry matter accumulation amount (DMA) at maturity by 22.97–56.25% and pre-flowering crop growth rate (CGR) by 17.11–92.85%, with no significant increase beyond 105 kg N ha−1. However, no significant correlation was observed between the dry matter translocation efficiency (DTE) and wheat grain yield. Both insufficient and excessive N applications resulted in an imbalanced N distribution favoring vegetative growth over reproductive growth, thus negatively impacting N-use efficiency. At maturity, the N-fertilized treatments significantly increased the two-year average N accumulation amount (NAA) by 52.04–129.98%, with no further increase beyond 105 kg N ha−1. N fertilization also improved the two-year average N translocation efficiency (NTE) by 56.89–63.80% and the N contribution proportion (NCP) of wheat vegetative organs by 27.79–57.83%, peaking in the lower-N treatment (N52.5). However, high-N treatment (N210) led to an increase in NO3-N accumulation in the 0–100 cm soil layer, with an increase of 26.27% in 2018 and 122.44% in 2019. This higher soil NO3-N accumulation in the 0–100 cm layer decreased NHI, NUE, NAE, NPFP, and NMB. Additionally, N fertilization significantly reduced the two-year average N harvest index (NHI) by 9.89–12.85% and N utilization efficiency (NUE) by 11.14–20.79%, both decreasing with higher N application rates. The NAA followed the trend of anthesis > maturity > jointing. At the 105 kg N ha−1 rate, the highest N agronomic efficiency (NAE) (9.31 kg kg−1), N recovery efficiency (NRE) (38.32%), and N marginal benefit (NMB) (10.67 kg kg−1) were observed. Higher dry matter translocation amount (DTA) and N translocation amount (NTA) reduced NHI and NUE, whereas higher NTE improved NHI, NUE, and N partial factor productivity (NPFP). Overall, N fertilization enhanced N-use efficiency in spring wheat by improving N translocation rather than dry matter translocation.

Fabrication of a Hydrophilic Al2O3/AlN EP Coating with Self‐Cleaning and High‐Efficiency Anti‐Corrosion Properties

AbstractCurrently, with the gradual development of anticorrosive coatings, the pursuit of simple preparation methods and the improvement of anticorrosive coatings’ properties have become the major research trends. This study successfully prepared a hydrophilic super anticorrosive coating through a simple mixing method. The coating has a contact Angle of 67.8° and a rolling Angle greater than 20°. This coating can withstand 180 days of salt spray test with no bubble or rust formation when placed on a Q 235 substrate in a 3.5 wt % NaCl solution, demonstrating the excellent corrosion resistance of the AO0.4AN0.6EP coating. Furthermore, after being immersed in a 3.5 wt % NaCl solution for 3 days, the AO0.4AN0.6EP coating exhibited the largest impedance diameter (2.07×108 Ω⋅cm2), almost three times larger than other coatings, and possessed the highest Bode modulus value, one order of magnitude higher than other coatings. After immersion in a 3.5 wt % NaCl solution for 7 days, the AO0.4AN0.6EP coating had the largest Ecorr and the smallest icorr, indicating its superior corrosion resistance. In addition to corrosion resistance, the AO0.4AN0.6EP coating showed no signs of cracking, discoloration, or decomposition after being exposed to ultraviolet aging lights for 30 days, demonstrating its excellent UV resistance. Moreover, neither dry soil particles nor muddy water could adhere to the coating surface, proving its good self‐cleaning ability. Therefore, this coating holds promising applications in the field of super corrosion‐resistant coatings.

Diversity in stomatal and hydraulic responses to post-flowering drought in common (Phaseolus vulgaris) and tepary (P. acutifolius) beans.

Plants differ widely in how soil drying affects stomatal conductance (gs) and leaf water potential (ψleaf), and in the underlying physiological controls. Efforts to breed crops for drought resilience would benefit from a better understanding of these mechanisms and their diversity. We grew 12 diverse genotypes of common bean (Phaseolus vulgaris L.) and four of tepary bean (P. acutifolius; a highly drought resilient species) in the field under irrigation and post-flowering drought, and quantified responses of gs and ψleaf, and their controls (soil water potential [ψsoil], evaporative demand [Δw] and plant hydraulic conductance [K]). We hypothesised that (i) common beans would be more "isohydric" (i.e., exhibit strong stomatal closure in drought, minimising ψleaf decline) than tepary beans, and that genotypes with larger ψleaf decline (more "anisohydric") would exhibit (ii) smaller increases in Δw, due to less suppression of evaporative cooling by stomatal closure and hence less canopy warming, but (iii) larger K declines due to ψleaf decline. Contrary to our hypotheses, we found that half of the common bean genotypes were similarly anisohydric to most tepary beans; canopy temperature was cooler in isohydric genotypes leading to smaller increases in Δw in drought; and that stomatal closure and K decline were similar in isohydric and anisohydric genotypes. gs and ψleaf were virtually insensitive to drought in one tepary genotype (G40068). Our results highlight the potential importance of non-stomatal mechanisms for leaf cooling, and the variability in drought resilience traits among closely related crop legumes.

Ground motion amplification by twin circular tunnels for obliquely incident seismic waves: Effects of saturated poroelastic soil parameters

Underground structures can significantly affect the ground motion and further influence the seismic design of aboveground structures around them. Although this issue has been well recognised and studied, it is not thoroughly addressed as a large number of factors are involved. This paper focuses on ground motion amplification induced by twin circular tunnels embedded in saturated poroelastic soil. The saturated poroelastic soil is modelled as a two-phase medium to consider parameters characterising the fluid phase. Three-dimensional scattering features of the twin tunnels for obliquely incident seismic waves are numerically investigated by a 2.5D finite element and boundary element hybrid model. It is shown that the presence of the twin tunnels can lead to significant modifications of the free-field motion, in terms of its magnitude and distribution. The three-dimensional oblique incidence scenario presents a more general perspective on the ground motion amplification. Moreover, the effects of saturated poroelastic soil parameters on the ground motion amplification, including soil permeability, groundwater levels, and the degree of saturation, are parametrically investigated. The differences in ground motion amplification patterns between the saturated poroelastic and dry poroelastic soil scenarios are notable, highlighting the effects of parameters characterising the fluid phase.

Competition limits first-year growth and flowering of wiregrass (Aristida beyrichiana) at a sandhills restoration site.

Uncertainty in ecosystem restoration can be mitigated by information on drivers of variability in restoration outcomes, especially through experimental study. In southeastern USA pine savannas, efforts to restore the perennial bunchgrass wiregrass (Aristida beyrichiana) often achieve variable outcomes in the first year. Although ecotypic differentiation and competition with other native vegetation are known to influence wiregrass seedling establishment and growth, to our knowledge, no studies have examined interactions between these drivers. We experimentally quantified individual and interactive effects of competition, seed source, and soil type on wiregrass density, size, and flowering culm production in the field. We sowed seeds from dry and wet sites reciprocally into dry and wet soils and weeded half of the plots. We found that competition removal resulted in significantly larger plants and a greater proportion of flowering plants with more culms on average, regardless of seed source or soil type. Seeds sourced from a wet site resulted in more plants per plot than seeds from a dry site, which might have been influenced by the greater number of filled seeds from the wet site. After seedlings become established, competition contributes to variation in growth and reproduction. Although competition removal could help start wiregrass populations, the necessity of mitigation depends on fire management needs.

Ensuring Soil Security to Secure Our Planetary Future$

Turning the dirt, we have cultivated back into soil is critical to securing our planetary future. The world’s principal existential challenges – food, water and energy security, climate change abatement, biodiversity protection and human health1 – are all underpinned by soil dysfunction. Yet, little is known about soil function, soil services and threats to soil, or how the state of our soils, determined by land use and land management, cause desertification and climate change. As greenhouse gases are transparent to incoming solar radiation, we must mitigate excess atmospheric heat by reducing the amount of organic matter mined from the soil, because when the sun’s rays fall on exposed, drained, baked dry soil, most of the solar radiation is converted from latent heat to sensible heat. Soil specific policy and legislation must be developed to regulate sources of excess sensible heat, consistent with limiting global temperature rise to 1.5°C above pre-industrial levels. Through soil security, we can deliver a better present and safeguard our planetary future.

A root mucilage analogue from chia seeds reduces soil gas diffusivity

AbstractGas exchange in the soil is determined by the size and connectivity of air‐filled pores. Root mucilage reduces air‐filled pore connectivity and thus gas diffusivity. It is unclear to what extent mucilage affects soil pore connectivity and tortuosity. The aim of this study was to gain a better understanding of gas diffusion processes in the rhizosphere by explaining the geometric alterations of the soil pore space induced by mucilage. We quantified the effect of a root mucilage analogue collected from chia seeds without intrinsic respiratory activity on oxygen diffusion at different water contents during drying–rewetting cycles in a diffusion chamber experiment. Quantification of oxygen diffusion showed that mucilage decreased the gas diffusion coefficient in dry soil without affecting air‐filled porosity. Without mucilage, a hysteresis in gas diffusion coefficient during a drying–rewetting cycle was observed. The effect depended on particle size and diminished with increasing mucilage content. X‐ray computed tomography imaging indicated a hysteresis in the connectivity of the gas phase during a drying–rewetting cycle for samples without mucilage. This effect was attenuated with increasing mucilage content. Furthermore, electron microscopy showed that mucilage structures formed in drying soil increase with mucilage content, thereby progressively reducing the connectivity of the gas phase. In conclusion, the effect of mucilage on soil gas diffusion highly depends on soil texture and mucilage content. The diminishing hysteresis with the addition of mucilage suggests that plant roots secrete mucilage to balance oxygen availability and water content, even under fluctuating moisture conditions.

Effects of Trichoderma harzianum and Bacillus subtilis on the root and soil microbiomes of the soybean plant INTACTA RR2 PRO™.

Soybean is a significant export product for several countries, including the United States and Brazil. There are numerous varieties of soybean. Among them, a genetically modified type known as INTACTA RR2 PRO™ has been designed to demonstrate resistance to glyphosate and to produce toxins that are lethal to several species of caterpillars. Limited information is available on the use of Trichoderma harzianum and Bacillus subtilis to promote plant growth and their impact on the plant microbiome. This study aimed to evaluate the effects of these microorganisms on this soybean cultivar by analyzing parameters, such as root and shoot dry matter, nutritional status, and root and soil microbial diversity. The results indicated that treatments with B. subtilis alone or in combination with T. harzianum as seed or seed and soil applications significantly enhanced plant height and biomass compared to the other treatments and the control. No significant differences in phosphorus and nitrogen concentrations were detected across treatments, although some treatments showed close correlations with these nutrients. Microbial inoculations slightly influenced the soil and root microbiomes, with significant beta diversity differences between soil and root environments, but had a limited overall impact on community composition. The combined application of B. subtilis and T. harzianum particularly enhanced plant growth and promoted plant-associated microbial groups, such as Rhizobiaceae, optimizing plant-microbe interactions. Furthermore, the treatments resulted in a slight reduction in fungal richness and diversity.

Thermo-hydro-mechanical behaviour of energy tunnel in unsaturated soils

Soils surrounding energy tunnels are often unsaturated, particularly in areas with a low water table. All studies of the mechanical behaviour of energy tunnels focused on saturated or dry soils. Through numerical simulations using a coupled thermo-hydro-mechanical model for unsaturated soils, this study aims to improve the understanding of the interactions between energy tunnels and unsaturated ground. The influences of water table position and soil type on the mechanical behaviour of energy tunnels were investigated. Results show that the thermally-induced lining convergence of energy tunnels does not change much with the water table position. Nevertheless, the other mechanical responses (e.g., settlements of ground and tunnel and lining stress changes) highly depend on the water table position. These mechanical responses are more significant when the water table is higher than the tunnel center, except for the larger settlements at a lower water table in clay. Furthermore, the above responses are underestimated if soils above the water table are assumed to be completely dry. The degree of underestimation is most significant for the clay, followed by silt and sand, e.g., a maximum of 54%, 23% and 10% in the change of circumferential stress, respectively. These results highlight that the unsaturated soil model should be used to evaluate the thermo-hydro-mechanical responses of energy tunnels in unsaturated soils, particularly for the clay ground.