Bean Research Articles

Determination of combining ability and heritability for canning quality traits in common bean

Breeding for common bean (Phaseolus vulgaris L.) genotypes with optimal canning quality holds the potential for increased dry bean consumption among middle-class income urban dwellers in sub-Saharan Africa (SSA). Understanding the genetic control of canning quality traits is central for the improvement of common bean genotypes for desired canning quality. The objective of this study was to determine the combining ability and heritability of canning quality traits (CQTs) in common bean; and to establish entry points for effective breeding for these traits. A population from two contrasting parents for canning quality traits was developed and progressed to F2. The F2:F4 seed was evaluated for CQTs at three months post-harvest. Substantial variation was observed among the canning quality traits tested, including the general appearance of the canned beans, seed coat colour retention namely, clumping, free starch, splitting, and hydration coefficient. Canning quality traits encompassed a spectrum of both additive and non-additive effects. Promising combiners for CQTs were identified among the parents, KK8 and MAC 13, suggesting their potential for utilisation in breeding programmes. Moderate to high Baker’s ratio (BR = 0.41-0.94) values were observed for some CQTs and narrow sense heritability (h2 = 0.47-0.66). Canning quality traits exhibited substantial broad sense heritability (H2) values, ranging from 0.6 to 0.92. These findings provide valuable entry points for breeding programmes focused on improving common bean for canning quality.

Factors affecting decisions of farmers to produce geographical indication dry beans: a case from Turkey

Geographical indications (GIs) are defined as labels that show the relationship of a product with a certain region. They are of great importance in terms of distinguishing these products from the similar ones in terms of the quality that the characteristic features of the region bring to the product, making them stand out commercially, contributing to the rural development and preserving the cultural heritage. Dry beans cultivated by the farmers in the Gümüşhane province is newly recognized as a GI product that is unique in both taste and quality. This study is intended to identify the factors affecting the farmers’ decision to produce GI-labelled dry beans. The survey was conducted in January–March 2021. A sampling size of the participants consisted of 50 farmers who cultivated GI-labelled dry beans and 50 non-GI dry beans. A binary logistic regression analysis approach was employed to identify the factors affecting the likelihood of the farmers cultivating the GI dry beans. The results of the model indicate that the young farmers with the low educational level, the low property land size, the low yield per decare, and the large farmland, the high annual agricultural income and specializing in the dry beans production are more likely to engage in the production of GI dry beans. For the farms involving the GI product, agricultural associations can be advised with the farmers with the wider agricultural acreage and the high annual agricultural income, and that specialize in the production of such products. The participation of the farmers in the agriculture enterprises involving the GI dry beans will depend on them seeing positive widespread effects, which requires a long-term commitment.

Effects of diet containing germinated faba bean (Vicia faba L.) on the intestinal health and gut microbial communities of Nile tilapia (Oreochromis niloticus)

Crisp Nile tilapia (Oreochromis niloticus) is a valuable fish product with high muscle firmness and chewiness generated by dietary faba beans (Vicia faba L.). However, the overconsumption of faba bean could induce intestinal inflammation in Nile tilapia due to its endogenous anti-nutritional factors (ANFs). Germination has been cited as an effective method for reducing ANFs in faba beans. But, the effects of germinated faba beans on the tilapia remain unclear. In this report, Nile tilapia were fed a diet supplemented with dry faba beans (FB0), or germinated faba beans (FB5), or a basal diet (control) for 75 days. Thereafter, samples of the fish were examined for textural quality, digestive enzyme activity, histology of the inner intestinal wall, expression of related genes, and composition of intestinal microbes. The hardest fillets were obtained from the FB5 (406.17 ± 42.51 gf) group, followed by the FB0 (301.17 ± 24.70 gf) and control (117.50 ± 8.19 gf) groups. The amylase levels were lower in the FB0 and FB5 groups, whereas the trypsin activities were up to 1.9-fold higher than in the control group. The gene expressions of IL-1β, CCL3, and TNF-α were significantly up-regulated in both the FB0 and FB5 groups, whereas metallothionein expression was down-regulated in the intestine relative to control group. Moreover, expression of IFN-γ and Hsp70 decreased in FB5, but increased in FB0. In particular, the diversity of intestinal microbiota was higher in the FB5 group than in the FB0 group (Fusobacteria abundance significantly decreased from 79.18% to 55.87%, while Proteobacteria abundance decreased from 8.12% to 7.02%), indicating that the intestinal microbiota structure was affected by altered diets. These results indicate that germinated faba bean could be a good substitute for dry faba beans in tilapia culture.

Analisis Implementasi Etika Jual Beli Kopi Dalam Islam di Desa Koto Rami Kecamatan Lembah Masurai Kabupaten Merangin

The purpose of this research is to find out how Islamic business ethics in Koto Rami Village, Lembah Masurai District, Merangin Regency, to find out whether business ethics in Koto Rami Village, Lembah Masurai District, Merangin Regency are in accordance with Islam, to find out what are the obstacles in implementing Islamic business ethics in the village. Koto Rami, Lembah Masurai District, Merangin Regency. This research is field in nature. The data collection techniques in this research are: observation, interviews, and documentation carried out by the coffee shop and coffee farmers. Then the existing data was analyzed using qualitative analysis. As for the research results, it was found that Islamic business ethics were carried out by the toke and farmers, toke who cheat by reducing the scale, coffee farmers mix coffee beans that still have a lot of water content with dry coffee beans, do not explain the actual condition of the goods so that it harms other people, there are still many toke who badmouth other toke with the motive of business competition , many farmers slander other farmers so that they don't buy the farmer's harvest. Analysis of the implementation of Islamic business ethics in Koto Rami Village, Lembah Masurai District, Merangin Regency is not in accordance with the principles of business ethics.

Adult dietary patterns with increased bean consumption are associated with greater overall shortfall nutrient intakes, lower added sugar, improved weight-related outcomes and better diet quality

BackgroundLimited evidence is available that focuses on beans within American dietary patterns and health. The purpose of this study was to identify commonly consumed adult dietary patterns that included beans and compare shortfall nutrient intakes and diet quality, relative to adults whose typical dietary pattern did not include beans.MethodsThe analyses used data from the National Health and Nutrition Examination Survey, 2001–2018. Cluster analysis was used to identify bean patterns of consumption, while the USDA food coding system defined daily beans consumed. Five bean dietary patterns of consumption were identified, of which four patterns included both canned beans and dry beans, while one pattern had no bean consumption. Bean consumption was defined as those consuming kidney beans, black beans, chickpeas, and/or pinto beans.ResultsAdults consuming Bean Dietary Patterns 1, 2, 3 and 4 had significantly higher diet quality scores (as assessed by USDA’s Healthy Eating Index-2015) compared to the no-bean pattern (61.2 ± 0.5, 58.9 ± 0.5, 55.2 ± 0.4 and 56.5 ± 0.8 vs 48.8 ± 0.2 p’s < 0.0001). Bean consumers also had significantly higher intakes of several shortfall nutrients (choline, alpha-linolenic acid, folate, iron, magnesium and vitamin E) relative to non-consumers of beans. Similarly, intake of dietary fiber, potassium and calcium, all nutrients of public health concern were significantly higher in bean patterns compared to no-beans. Bean Dietary Pattern 1 (~ 13.5% of total daily kcal from beans or ~ 2 servings of beans/day) and 2 (~ 9.5% of total daily kcal from beans or ~ 1.7 servings of beans/day) were significantly associated with lower BMI, decreased body weight and improved waist circumference relative to no-beans.ConclusionsDietary patterns that are rich in canned and dry beans were associated with significantly higher diet quality scores and greater intake of shortfall nutrients, including nutrients of public health concern. Bean dietary patterns were also associated with improved weight-related outcomes. Dietary guidance should consider the nutrient and health benefits associated with the promotion of increased canned and dry bean consumption in American dietary patterns.

A Study of Physical, Chemical, and Sensory Characteristics of Novel Legume Dips.

There is a consensus among experts and consumers that pulses are a good source of nutrients and fiber. In a traditional hummus recipe, chickpeas are the major ingredient. The present study is aimed at developing new legume dips by exchanging chickpeas (Chd) with dry green (Gld) and red lentils (Rld), dry white beans (Wbd), and dry green peas (Gpd). Presoaking, boiling, proximate composition, pH, energy, color measurement, and sensory evaluation were conducted on the dips using chickpea dip (hummus) as a control. One-way ANOVA was used to determine the differences between the dips. The results revealed significant differences in the proximate composition of legume dips. The protein content of the five samples ranged between 7.46% and 9.19%, while the values varied from 8.59% to 10.93% in fat, 3.88% to 6.54% in crude fiber, 14.48% to 15.51% in carbohydrates, 171.95 to 195.13 in energy, 1.55% to 1.76% in ash, and 63.35% to 66.90% in moisture. These variations could be attributed to the type and composition of each legume, the soaking and boiling process, and the tahini added during the preparation. pH ranged between 4.5 and 4.7. The color measurement indicated that the five legume dips could be considered bright products (high L∗>67), with a positive color valuebluered-green and yellow-. Significant differences (p ≤ 0.05) were observed in the legume dips sensory evaluation, and the red lentil dip was the most acceptable with results comparable to the chickpea dip; it was followed by the green lentil, white bean, and green pea dips. These results highlight the feasibility of commercial production of legume dip that promotes human health and gives consumers more choices.

Economic impact of local geographical indication products on farmers: a case of Kelkit sugar (dry) beans

Economic impact of local geographical indication products on farmers: a case of Kelkit sugar (dry) beans

Life cycle inventory and assessment of Canadian faba bean and dry bean production

Pulses are nutritionally beneficial and cost-effective food/feed products, and are also nitrogen-fixing crops that can improve the environmental sustainability of annual cropping systems when included in crop rotations – particularly with respect to GHG emissions. Canada is a major producer and exporter of pulses, therefore the goals of this study were to (a) develop regionalized life cycle inventories (LCIs) for Canadian faba and dry beans suitable for incorporation into public LCI databases and (b) conduct ISO 14044-compliant LCAs of Canadian faba, navy, pinto, and kidney beans at provincial, ecozone, and national scales. LCI data were collected via survey from ~200 Canadian faba and dry bean farmers.At the national scale of aggregation, faba beans had considerably lower impacts across all impact categories considered than the three dry bean types. This is due to their lower input requirements (particularly fertilizer), and higher nitrogen fixation capabilities. Among the three dry bean types considered, pinto beans had the lowest environmental impacts across most impact categories. Fertilizer production and field-level emissions from fertilizer use were the major contributors to the overall environmental impacts of the production of all bean types. In addition to differences between bean types, there were also differences within bean types, between regions of production and levels of regional aggregation. The results of the uncertainty analysis revealed that variability was lowest when aggregated at the ecozone level, based on soil and climate factors, and highest when aggregated to the national average. Taken together, these results demonstrate the utility of spatially-resolved LCI and LCA modelling that distinguishes between different types of pulse crops in order to accurately estimate impacts, and to support decision-making in pursuit of more sustainable food systems. Future research needs include more primary data on field-level biological nitrogen fixation and soil carbon fluxes, since these can significantly influence estimated environmental impacts.

Exploring the Mycovirus Sclerotinia sclerotiorum Hypovirulence-Associated DNA Virus 1 as a Biocontrol Agent of White Mold Caused by Sclerotinia sclerotiorum.

Sclerotinia sclerotiorum is a necrotrophic fungal pathogen causing white mold on many important economic crops. Recently, some mycoviruses such as S. sclerotiorum hypovirulence-associated DNA virus 1 (SsHADV-1) converted S. sclerotiorum into a beneficial symbiont that helps plants manage pathogens and other stresses. To explore the potential use of SsHADV-1 as a biocontrol agent in the United States and to test the efficacy of SsHADV-1-infected United States isolates in managing white mold and other crop diseases, SsHADV-1 was transferred from the Chinese strain DT-8 to United States isolates of S. sclerotiorum. SsHADV-1 is readily transmitted horizontally among United States isolates of S. sclerotiorum and consistently conferred hypovirulence to its host strains. Biopriming of dry bean seeds with hypovirulent S. sclerotiorum strains enhanced resistance to white mold, gray mold, and Rhizoctonia root rot. To investigate the underlying mechanisms, endophytic growth of hypovirulent S. sclerotiorum in dry beans was confirmed using PCR, and the expression of 12 plant defense-related genes were monitored before and after infection. The results indicated that the endophytic growth of SsHADV-1-infected strains in plants stimulated the expression of plant immunity pathway genes that assisted a rapid response from the plant to fungal infection. Finally, application of the seed biopriming technology with SsHADV-1-infected hypervirulent strain has promise for the biological control of several diseases of wheat, pea, and sunflower.

Assessment of crop water requirement and irrigation scheduling for selected crops in the Kohima district, Nagaland, India

Abstract This study was done to examine agricultural water and irrigation schedules for key crops grown in Kohima area of Nagaland, India, which has a humid subtropical climate. Climatic data over 16 years (2006–2021) were used to estimate crop water requirements using CROPWAT 8.0 model. Rice, maize, soybean, potato, cabbage, dry bean, ginger, and naga chili are the most common crops. ET0 was calculated using the FAO Penman–Monteith method and ranged from 1.41 to 3.65 mm/day. The USDA SCS approach calculated effective rainfall. The average annual ETC for rice, maize, ginger, soybean, bean (dry), potato, cabbage, and naga chili were 537.1, 305.7, 342.7, 292.2, 288.1, 364.3, 190.6, and 141 mm, respectively. Rice, maize, ginger, soybean, bean (dry), potato, cabbage, and naga chili require an average of 251.7, 54.9, 26, 73.1, 21.3, 21.9, 121.9, and 14.5 mm of irrigation each year, respectively. The crop irrigation schedules were created with 70% efficiency. The study emphasizes that irrigation must be adjusted to each crop's individual needs, whether through strategic scheduling or modifying net and gross irrigation volumes, to enhance water management and maximize crop production in the region.

TOTAL ANTIOXIDANT CAPACITY MAY NOT BE AN ACCURATE WAY TO PREDICT DENTAL CARIES

Salivary biomarkers of oxidative stress in children with dental caries: Systematic review and meta-analysis doi:10.1016/j.archoralbio.2022.105432. CAPES (Coordination for the improvement of higher education, National Council of Technological and Scientific Development), Finace code 001. Systematic review with meta-analysis of data.

Physicochemical and Sensory Properties of Arabica Coffee Beans of Arara cv. Dried Using Different Methods.

The coffee fruit is preferably harvested at the cherry stage, with high moisture and metabolic activity, and must then undergo a drying process for better preservation of the bean and its sensory attributes. In this context, this study aimed to characterize the final quality of the Arara cultivar Arabica coffee processed using the wet method and subjected to six drying methods: three conducted at the agro-industrial establishment (fixed-bed dryer, rotary drum dryer, and combined drying) and three laboratory-scale methods (convective oven, cast-tape drying, and suspended terrace). Drying was carried out to reduce the coffee's moisture content from an initial value of 46.2% on a wet basis (w.b.) to a final average value of 11.35% (w.b.). The fruits of in natura demucilaged coffee and the processed dry coffee beans were characterized for moisture, ash content, nitrogen compounds, lipids, total titratable acidity, organic acids, sugars, and the instrumental color of the beans. The sensory profile of the Arabica coffee was evaluated by five coffee specialists using the methodology proposed by the Specialty Coffee Association (SCA), and all the coffees were classified as a specialty.

Biochemical composition of the beans of local coffee cultivars from southwestern Saudi Arabia

Coffee (Coffea arabica L.) is an economically and culturally important crop for the communities of the southwest mountain regions of Saudi Arabia. The objective of the study was to characterize the biochemical composition of the dry green beans of the coffee cultivars grown there. The analysis of variance revealed significant differences among the 66 accessions studied for all eight biochemical compounds analyzed especially, caffeine, trigonelline and chlorogenic acid. Remarkably, caffeine concentrations varied from 0.75 g 100 g−1 in accession KSA-38–1.51 g 100 g−1 in KSA-47 and trigonelline concentrations varied from 0.81 g 100 g−1 in KSA-69–1.64 g 100 g−1 in KSA-6R. However, 55% and 64% of the accessions had low to moderate caffeine and trigonelline concentrations, respectively. Chlorogenic acid concentration varied from 2.35 g 100 g−1 in KSA-3R to 4.33 g 100 g−1 in KSA-32. Because these compounds are the most important bitterness attributes contributing to coffee quality, significant differences in beverage quality are expected between the accessions. Furthermore, the results of the multivariate analysis indicated the presence of considerable diversity among the 66 accessions. The hierarchical cluster analysis grouped the genotypes into fourteen clusters. The observed divergence suggests that genetic gains for biochemical features are possible. Therefore, we recommend accessions KSA-1, KSA-9R, KSA-10, KSA-29, KSA34, KSA37, KSA-38, KSA-39 and KSA-60 for further evaluation.

A retrospective analysis of historical data of multi‐environment trials for dry bean (Phaseolus vulgaris L.) in Michigan

AbstractPlant breeding programs use multi‐environment trials (METs) to test multiple genotypes with the aim of selecting superior lines and increasing genetic gain. However, METs can be affected by the genotype × environment interactions (GEI) which hinders the ability to identify stable and high‐yielding dry bean varieties. This study investigates the impact of GEI on grain yield (GY) in three dry bean market classes across multiple environments in Michigan. The goal is to identify genotypes with superior performance under GEI effects and describe the genetic correlation between locations and other agronomic traits. Furthermore, we evaluate genetic yield trends on three major dry bean market classes in Michigan. Using linear mixed models, stability analysis, and a multi‐trait index, this study accounted for the effects of the environment on genotype performance to improve the prediction of GY. Our results highlight the importance of considering GEI in dry bean breeding programs to enhance the selection accuracy of GY. The genotype by environment interaction biplot analysis and the GEI model aided in identifying and classifying mega‐environments, leading to specific strategies for testing commercial bean classes across target production environments. Multiple‐trait performance selection showed positive results, although limited gains were observed for early maturing genotypes. Finally, the analysis of genetic trends reveals a positive trend in yield for black beans, while navy beans and small red beans show a slight decrease which can be attributed to unfavorable growing conditions and lack of genetic diversity in these market classes.

Sensory properties of selected biofortified common bean (Phaseolus vulgaris) varieties grown in Burundi.

The dry common bean is an important grain legume used for human consumption worldwide. In Eastern Africa, Burundi has a significantly high per capita consumption of the crop. There has been significant research on the underlying agronomic traits of dry biofortified common beans, such as disease resistance. However, there is limited systematic information describing the sensory properties of these bean varieties, particularly in Burundi. This study evaluated the sensory properties of eight cooked dry biofortified common bean varieties using a panel of fifty-four (fourteen plus forty) persons for descriptive sensory evaluation and consumer acceptability tests. Kinure, a traditional non-biofortified common bean variety, was the control. Based on differences in the attributes of the bean varieties, two-dimensional principal component analysis (PCA) explained 58.94% of the variation. The attributes of astringency, consistency, color, juiciness, beany aroma, stickiness, and bean size contributed mostly to the differentiation of the bean varieties. A 95% PCA prediction ellipse displayed stronger congruity in the descriptive attributes of NUV130, NUV91, RWV1129, RWV1272, and RWR2245. In contrast, a deviation in the descriptive attributes of MAC44, MAC70, and RWR2154 was discerned. Regarding consumer acceptability tests, the varieties RWR2245 and MAC44 garnered significantly higher (p < .05) sensory scores on color, aroma, taste, texture, and overall acceptability. Therefore, the physical traits of cooked biofortified common bean varieties are a major contributor to varietal disparities in consumer acceptance studies. These parameters can greatly impact the adoption of dry biofortified common beans and could be of concern to common bean breeders.

NUA 45, a dry bean cultivar, builds a subtle brand in Zimbabwe against all odds

Global business today is driven by brands, each with a unique story, but rarely of a product that is a public good. Building a brand requires careful planning, creativity, and an understanding of the target market. Borrowing from various brand concepts and frameworks, this paper explores how a government-bred common bean cultivar., NUA 45, defied the odds to create a new identity and association in Zimbabwe. Firstly, the cultivar overcame the general lack of promotion investment in government-bred (public) common bean cultivars. Secondly, being a red mottle-colored cultivar in a red-speckled (sugar type) bean-predominated country, NUA 45 had to break the preference typecasts. Thirdly, NUA 45 was among the first generation of biofortified bean cultivars enriched with iron (Fe) and zinc (Zn), some of the most sought-after micronutrients in a world ridden with hidden hunger. Overcoming these market barriers required a systematic approach to awareness creation, collaboration, and commercialization, avoiding the tragedy of the commons, and instead, achieving a win-win situation for all stakeholders. This entailed understanding the needs and preferences of different value chain actors, developing messages, and engaging local farmers, retailers, aggregators, and private and public sector partners to promote NUA 45. Lastly, developing compelling stories and leveraging digital channels to reach new customers that sought personal experiences, moved the cultivar name from a mere label to a spontaneously endorsed lifestyle and a brand that ‘spread like wildfire’ across the country and the Southern Africa Development Community (SADC) region. The NUA 45 brand broke new ground for other biofortified bean cultivars and non-sugar bean cultivars. Following its release in seven other countries, NUA 45 pioneered listing on the SADC regional variety catalog.

Genome-wide association and genomic prediction for iron and zinc concentration and iron bioavailability in a collection of yellow dry beans.

Dry bean is a nutrient-dense food targeted in biofortification programs to increase seed iron and zinc levels. The underlying assumption of breeding for higher mineral content is that enhanced iron and zinc levels will deliver health benefits to the consumers of these biofortified foods. This study characterized a diversity panel of 275 genotypes comprising the Yellow Bean Collection (YBC) for seed Fe and Zn concentration, Fe bioavailability (FeBio), and seed yield across 2years in two field locations. The genetic architecture of each trait was elucidated via genome-wide association studies (GWAS) and the efficacy of genomic prediction (GP) was assessed. Moreover, 82 yellow breeding lines were evaluated for seed Fe and Zn concentrations as well as seed yield, serving as a prediction set for GP models. Large phenotypic variability was identified in all traits evaluated, and variations of up to 2.8 and 13.7-fold were observed for Fe concentration and FeBio, respectively. Prediction accuracies in the YBC ranged from a low of 0.12 for Fe concentration, to a high of 0.72 for FeBio, and an accuracy improvement of 0.03 was observed when a QTN, identified through GWAS, was used as a fixed effect for FeBio. This study provides evidence of the lack of correlation between FeBio estimated in vitro and Fe concentration and highlights the potential of GP in accurately predicting FeBio in yellow beans, offering a cost-effective alternative to the traditional assessment of using Caco2 cell methodologies.

Multiphasic investigations imply transfer of orange-/red-pigmented strains of the bean pathogen Curtobacterium flaccumfaciens pv. flaccumfaciens to a new species as C. aurantiacum sp. nov., elevation of the poinsettia pathogen C. flaccumfaciens pv. poinsettiae to the species level as C. Poinsettiae sp. nov., and synonymy of C. albidum with C. citreum

Curtobacterium flaccumfaciens (Microbacteriaceae), a plant-pathogenic coryneform species includes five pathovars with valid names and a number of proposed – but unvalidated – new members. In this study, phenotypic features and DNA similarity indexes were investigated among all C. flaccumfaciens members. Results showed that the C. flaccumfaciens pv. poinsettiae strains causing bacterial canker of Euphorbia pulcherrima in the USA as well as the orange-/red-pigmented strains of C. flaccumfaciens pv. flaccumfaciens pathogenic on dry beans in Iran are too distinct from each other and from the type strain of the species to be considered members of C. flaccumfaciens. Hence, the latter two groups were elevated at the species level as C. poinsettiae sp. nov. (ATCC 9682T = CFBP 2403T = ICMP 2566T = LMG 3715T = NCPPB 854T as type strain), and C. aurantiacum sp. nov. (50RT = CFBP 8819T = ICMP 22071T as type strain). Within the emended species C. flaccumfaciens comb. nov., yellow-pigmented strains causing bacterial wilt of dry beans and those causing bacterial canker of Euphorbia pulcherrima in Europe were retained as C. flaccumfaciens pv. flaccumfaciens and C. flaccumfaciens pv. poinsettiae, respectively; while taxonomic position of the sugar beet pathogen C. flaccumfaciens pv. beticola ATCC BAA144PT was confirmed. The newly described onion pathogen C. allii was also reclassified as C. flaccumfaciens pv. allii with the pathotype strain LMG 32517PT. Furthermore, C. flaccumfaciens pv. basellae causing bacterial leaf spot of malabar spinach (Basella rubra) was transferred to C. citreum pv. basellae with ATCC BAA143PT as pathotype.

First Report of Charcoal Rot Caused by Macrophomina phaseolina on Dry Bean (Phaseolus vulgaris L.) in Western Canada.

In 2021, several dry bean (Phaseolus vulgaris L.) plants at the mid-seed-fill growth stage displaying wilting, chlorosis of the leaves and reduced vigor were collected near the Pembina - Emerson Border of Manitoba, Canada and North Dakota, USA. When symptomatic plants were examined, gray to dark brown discoloration was observed on the lower stem and the roots. Afterwards, brown to black discoloration was noticed on stem and root sections. Root and lower stem pieces (1 to 2 cm) from affected plants were surface sterilized with 70 % ethanol, followed by 1% NaOCl, rinsed twice in sterilized water, air dried on sterilized filter papers, and placed on potato dextrose agar (PDA) amended with 1 mg/mL of streptomycin sulfate. The PDA plates were incubated at 28°C with 12 h light/12 h dark for 10 days. The growing hyphae were transferred using the hyphal tip method to new PDA plates. Growing cultures were initially hyaline and turned from light gray to dark brown or black with age. Abundant dark and spherical to oblong shaped sclerotia with an average diameter of 97.9 µm (range: 66.8 to 143.5 µm, n =30) formed on the pure cultures 7 days after incubation. Additional pure culture was obtained through an isolation of a single microsclerotium followed by a single hyphal tip transfer. One isolate was identified as Macrophomina phaseolina based on morphological characteristics (Smith and Wyllie 1999). The morphological identity was confirmed by sequencing the rDNA internal transcribed spacer (ITS) region with universal primers ITS1/ITS4 (White et al. 1990) and calmodulin (CAL) and translation elongation factor-1 alpha (TEF-1α) genes with MpTefF/MpTefR, and MpCalF/MpCalR primer sets (Santos et al. 2020), respectively. The online resource Basic Local Alignment Search Tool (BLAST; https://www.ncbi.nlm.nih.gov/BLAST) confirmed the fungus identity as 100% M. phaseolina. The sequences of the original isolate BF21-25 were deposited in GenBank with accession numbers OQ615297 (ITS), OR357630 (CAL), and OR363106 (TEF-1α). To confirm pathogenicity, bioassays were conducted under controlled conditions. Four seeds of cultivar 'Etna' were sown per pot, and five pots were used for inoculated (approx. 4 × 105 microsclerotia/pot) and control (mock-inoculated with sterile PDA medium) treatments. For the inoculum, 20 g of macerated 10 to 14-day old M. phaseolina culture grown on PDA medium was applied to each pot using an inoculum layering technique. Pots were kept in the greenhouse with 28/17°C day/night, 13 h light/11 h dark cycle, and 70% relative humidity and watered weekly. Disease symptoms similar to those observed in the field were visible on all inoculated plants at the mid-seed-fill growth stage. Mock-inoculated control plants didn't show any symptoms. The experiment was repeated twice with similar results. The pathogen was re-isolated from infected plants to confirm Koch's postulates and identified as M. phaseolina based on the morphology and sequences of ITS, CAL and TEF-1α regions. To our knowledge, this is the first report of charcoal rot caused by M. phaseolina on dry bean in Western Canada.

Arabica coffee yields at various harvest seasons and altitudes in the Gayo Highlands, Aceh

Arabica coffee yields in the Gayo Highlands, Aceh, are limited by altitude. Areas suitable for coffee are an altitude of 1000-1600 m asl. Climate changes, especially rising temperatures, result in vulnerability to Arabica coffee yields. This study aims to study coffee yields at various locations based on altitudes and harvest seasons. This study used a split-plot design, with 3 replications. The main plot is two season harvests (S) (March-June Harvest and September-December Harvest), and the sub-plot is six locations based on altitudes in Bener Meriah (BM) and in Aceh Tengah (AT), i.e.: BM>1000-1200 m asl, BM>1200-1400 m asl, BM >1400-1600 m asl, AT>1000-1200 m asl, AT>1200-1400 m asl, and AT >1400-1600 m asl. The season harvests did not affect all coffee yield parameters. Altitudes significantly affected the average number of coffee cherries/plant, the average weight of 100 dry coffee beans, and the average weight of dry coffee beans/plant but did not affect the average weight of coffee cherries/plant. The combination of harvest seasons and altitudes (locations) shows that in the March-June harvest season, location BM>1200-1400 m asl results in a better average weight of 100 dry coffee beans than September-December harvest season, while in AT>1200-1400 m asl is the other way around.