Azotobacter is free living, non–symbiotic, heterotrophic bacteria capable of fixing an average of 20kg N / hector year. Azotobacter species are Gram negative, free–living, aerobic soil dwelling, oval or spherical bacteria that form thick–walled cysts (means of asexual reproduction under favorable condition). Out of six species of Azotobacter, some of them are motile by means of peritrichous flagella while others are not. They are typically polymorphic and their size ranges from 2–10µm long and 1–2µm wide. A. chroococcum is the first aerobic free– living nitrogen fixer. These bacteria utilize atmospheric nitrogen gas for their cell protein synthesis. Dichlorvos is an organophosphate widely used as an insecticide to control household pests, in public health, and protecting stored products from insects. In present study of dichlorvos in Azotobacter through different doses are effected of seed germination and observed the toxicity and harmful effect of dichlorvos solution.