Gamma-ray Dose Research Articles

Radiation-Induced Wavelength Shifts in Fiber Bragg Gratings Exposed to Gamma Rays and Neutrons in a Nuclear Reactor.

Fiber Bragg gratings (FBGs) inscribed by UV light and different femtosecond laser techniques (phase mask, point-by-point, and plane-by-plane) were exposed-in several irradiation cycles-to accumulated high doses of gamma rays (up to 124 MGy) and neutron fluence (8.7 × 1018/cm2) in a research-grade nuclear reactor. The FBG peak wavelengths were measured continuously in order to monitor radiation-induced shifts. Gratings inscribed on pure silica core fibers using near-IR femtosecond pulses through a phase mask showed the smallest shifts (<30 pm), indicating that these FBGs are suitable for temperature measurement even under extreme ionizing radiation. In contrast, the pointwise inscribed femtosecond gratings and a UV-inscribed grating showed maximal shifts of around 100 pm and 400 pm, respectively. Radiation-induced red shifts are believed to arise from gamma radiation damage, which may partially recover after irradiation is stopped. At the highest neutron exposures, grating peak blue shifts started to appear, apparently due to fiber compaction.

Response of melon accessions to doses of Co60 gamma rays and their effects on the morphology of the M1 generation

Response of melon accessions to doses of Co60 gamma rays and their effects on the morphology of the M1 generation

Determining the optimal gamma irradiation dose for developing novel cowpea (Vigna unguiculata) genotypes

Purpose Cowpea (Vigna unguiculata (L.) Walp.) is a major legume crops for human consumption and livestock feed in tropical regions. Although its importance, the crop’s production is subjected to numerous constraints, raising the need to develop outstanding genotypes. In this line, this study assesses the effects of gamma irradiation doses on cowpea genotypes to determine the LD50 and its effects on agro-morphological parameters. Materials and methods Healthy dry seeds of three cowpea genotypes, Bambey 21, Me51-M4-39M9, and Ndout violet pods (VP), were exposed to four doses of gamma-rays 0, 200, 300, and 400 Gy. Qualitative and quantitative parameters were evaluated since germination, and a two-way analysis of variance (ANOVA) was performed on the means of quantitative traits using R software. Results The results revealed that the LD50 on seed germination and plant survival ranged from 579 and 446.25 Gy, in Me51-M4-39M9, respectively. These values varied significantly among genotypes and revealed that low doses of gamma irradiation stimulated germination speed and had a positive effect on the early flowering of Ndout VP. Higher doses of gamma irradiation induced more severe mutations, causing visible effects such as changes in leaf color (albino, xantha, viridis, and variegation) and phyllotaxis. The frequency of chlorophyll mutants induced by gamma irradiation was found to be dose-genotype/dependent, with Bambey 21 being the most sensitive variety. Lower doses induced desirable mutations such as stem pigmentation and seed hilum coloration on Bambey 21 and Me51-M4-39M9. It has also had a positive impact on seedling height and leaf number in Bambey 21 and Ndout VP. However, high irradiation doses lead to a significant reduction in certain quantitative traits, such as plant height (PH), leaf length (LL), leaf width (LW), pod width (PWD), pod weight (PW), seed width (SWD), and seed weight. Analysis of the phenotypic performance of quantitative traits allowed us to cluster the four doses by genotype into three groups. Conclusions The optimum dose of gamma-irradiation in cowpea mutation breeding is genotype-dependent. The effects of gamma-irradiation on these traits and their relationships are highly dependent on the specific crop and genotype. Further research is needed to understand these effects underlying mechanisms and develop crop improvement strategies using gamma irradiation.

Dose optimization of extended collimators in boron neutron capture therapy

In this paper, we propose the design of extending collimators aimed at reducing the radiation dose received by patients with normal tissues and protecting organs at risk in Boron Neutron Capture Therapy (BNCT). Three types of extended collimators are studied: Type 1, which is a traditional design; Type 2, which is built upon Type 1 by incorporating additional polyethylene material containing lithium fluoride (PE(LiF)); Type 3, which adds lead (Pb) to Type 1. We evaluated the dose distribution characteristics of the above-extended collimators using Monte Carlo methods simulations under different configurations: in air, in a homogeneous phantom, and a humanoid phantom model. Firstly, the neutron and gamma-ray fluxes at the collimator outlet of the three designs showed no significant changes, thus it can be expected that their therapeutic effects on tumors will be similar. Then, the dose distribution outside the irradiation field was studied. The results showed that, compared with Type 1, Type 2 has a maximum reduction of 57.14% in neutron leakage dose, and Type 3 has a maximum reduction of 21.88% in gamma-ray leakage dose. This will help to reduce the radiation dose to the local skin. Finally, the doses of different organs were simulated. The results showed that the neutron dose of Type 2 was relatively low, especially for the skin, thyroid, spinal cord, and left lung, with the neutron dose reduced by approximately 20.34%, 16.18%, 26.05%, and 18.91% respectively compared to Type 1. Type 3 collimator benefits in reducing gamma-ray dose for the thyroid, esophagus, and left lung organs, with gamma-ray dose reductions of around 10.81%, 9.45%, and 10.42% respectively. This indicates that attaching PE(LiF) or Pb materials to a standard collimator can suppress the dose distribution of patient organs, which can provide valuable insights for the design of extended collimators in BNCT.

Effect of Gamma Rays Irradiation on Seed Germination and Seedling Parameters in Sesame (Sesamum indicum L.)

The present investigation was carried out with the aim to understand the gamma ray sensitivity of three sesame varieties viz., DS-5, DSS-9 and Swetha irradiated with ten different doses of gamma rays ie., 250Gy to 700 Gy at 50 Gy intervals. Germination percentage, root and shoot length, seedling length decreased gradually in all the three varieties compared to control which is untreated. Swetha showed a maximum reduction in germination percentage (73.0%) than DSS-9 (74.0%) and DS-5 (76.5%) at 700 Gy. At a higher dose of 700 Gy, root 1(.17 cm) in DS-5 and shoot (2.47 cm) length of seedlings in DSS-9 were greatly inhibited. Based on all the biological parameters studied, the mutagen sensitivity of DSS-9 is higher than DS-5 and Swetha. The overall considerations on M1 generation effects showed that Swetha was least sensitive to gamma rays.

Design of Moderator Assemblies for Fast, Epithermal, or Thermal Neutron–Dominated n-γ Mixed Fields Using a D-D Neutron Source

The treatment field of boron neutron capture therapy (BNCT) is a n-γ mixed field. In the Osaka University BNCT project, a material-filtered radio-photoluminescence glass dosimeter (RPLGD) was proposed for the simultaneous measurement of neutron and gamma-ray doses. In this study, to validate the material-filtered RPLGD, various types of n-γ mixed fields are designed by irradiating different moderator assemblies with a D-D neutron source at the OKTAVIAN facility, Osaka University, Japan. The n-γ mixed fields are classified into fast neutron–, epithermal neutron–, or thermal neutron–dominated fields and a gamma-ray-only field with the specific characteristics as follows: (1) the dose ratios of gamma ray to neutron are 1.0% to 977.0% for the fast neutron–dominated field, 5.0% to 921.1% for the epithermal neutron–dominated field, 0.7% to 946.3% for the thermal neutron–dominated field, and 11880.6% for the gamma-ray-only field; (2) the proportions of fast, epithermal, and thermal neutron doses to total neutron dose are 98.4% to 100.0% for the fast neutron–dominated field, 74.0% to 85.4% for the epithermal neutron–dominated field, and 90.1% to 90.8% for the thermal neutron–dominated field, respectively; and (3) the maximum gamma-ray energy is up to 12 MeV.

Effect of gamma rays induced mutagenesis on the agronomical and biochemical traits of acid lime

One of the indigenous citrus crops of India is acid lime, which has a high economic value and unique flavour compared to other citrus fruits. The current study was intended to ascertain the gamma ray dosage that proves lethal for the acid lime variety PKM1. The acid lime seeds were exposed to nine distinct gamma radiation dosages ranging from 5 to 45 Gy with an interval of 5 Gy and compared to untreated control. Different irradiation doses demonstrated substantial differences in morphological and biochemical traits. Increased dosage of gamma radiation leads to significant changes in various agronomic parameters of plant germination, number of plants, plant height, and change in biochemical traits. Various gamma radiation doses revealed a low survival rate after 35 Gy, with LD 50 at 27.86 Gy.

Analysis of the Effects of Neutron Radiation on Cellulose Linen Fabrics Using Non-Destructive Testing.

This work describes the effects of using neutron irradiation on cellulose and non-destructive methods to analyze linen fabrics of high heritage value. For this purpose, 8 samples were irradiated with increasing doses of neutrons and gamma rays up to 166 kGy of total dose. The samples were characterized by techniques such as ultraviolet luminescence, attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy, Raman spectroscopy, and the nuclear magnetic resonance (NMR) technique. The luminescence of linen fabrics in the ultraviolet range increases markedly with dosage. Some chemical changes were also perceived by the ATR-FTIR spectra in the linen samples. Similarly, the fluorescence background observed in Raman spectroscopy intensifies with dosage. Due to the heterogeneity of the textile cellulose, NMR offers limited applicability for detecting neutron doses in cultural heritage fabrics. Radiation is applied for preservation against microorganisms in cultural heritage objects where the damage is to be assessed. This radiation can occasionally be found after earthquakes or in volcanic archaeological sites, which could question its dating using carbon 14. Despite some limitations encountered due to working with commonly used linen fabrics, the techniques employed in this study have made it possible to observe trends between the responses obtained and the irradiation dose for each linen sample.

Genetic and phytochemical evaluation of M2 generation mutants of fenugreek (Trigonella foenum-graecum L.) induced by gamma rays and Ethyl Methane Sulphonate (EMS).

Fenugreek (Trigonella foenum-graecum L.) is highly esteemed for its therapeutic properties and is widely used in traditional medicine and modern pharmacology. Enhancing its genetic traits and phytochemical profile, particularly its trigonelline content, can significantly increase its medicinal and agricultural value. This study aims to investigate the effects of gamma rays and Ethyl Methane Sulphonate (EMS) as mutagenic agents on the genetic and phytochemical characteristics of the M2 generation of fenugreek, focusing on genetic diversity and desirable trait enhancement. To achieve this, various concentrations of EMS and gamma rays were administered to fenugreek seeds, and 27 traits were assessed in the resulting M2 generation. These traits were analyzed for variance, mean values, and correlations. The genetic diversity of 23 M2 offspring was investigated using nine Start Codon Targeted (SCoT) markers. The genetic diversity assessment involved Principal Coordinate Analysis (PCoA) and cluster analysis, utilizing the Dice similarity coefficients and the Unweighted Pair Group Method with Arithmetic Mean (UPGMA). A Bayesian model provided deeper insights into the genetic structure. Results revealed that lower doses of gamma rays (100Gy) and EMS (0.2%) positively impacted specific traits. In comparison, higher doses (200Gy and 0.4% EMS) increased seed trigonelline content to 0.71mg/g dry weight. Among the SCoT markers, SCoT-9 was the most efficient, segregating the populations into three clusters. The first three principal components in the PCoA explained 20% of the total variance, leading to seven subgroup populations distinction. These findings underscore the potential of induced mutagenesis in enhancing desirable traits in fenugreek.

The effects of antioxidant administration in the early stages of radiation-induced tumorigenesis.

ApcMin/+ mouse was a model mouse for human familial adenomatous polyposis, and irradiation at an early age increases tumors in the small and large intestine. To study the effects of antioxidant administration on tumor incidence after continuous whole-body exposure to gamma rays, ApcMin/+ mice were exposed to a medium-dose-rate, 200mGy/d, from postnatal Day 0 to 21 of age or a high-dose-rate of 0.65Gy/min (total dose 4.2Gy) on postnatal Day 7. The dams and pups were supplied with the N-acetylcysteine (NAC) in drinking water (7g/L), from gestation Day 15 until weaning (21days-old). A significant increase in the number of intestinal tumors were observed in ApcMin/+ mice irradiated with high dose-rate gamma rays as compared with the non-irradiated controls, but there was no significant difference in tumor counts between the non-irradiated controls and the medium-dose rate irradiation groups. NAC administration did not have any significant effect at least at this dose. These results suggest that the supplementation of anti-oxidant at the early stage of tumorigenesis does not suppress the formation of irradiation-induced small intestinal tumors.

Isolation and characterization of gamma rays induced mutants for improved agro-morphological performance and harder grain texture in wheat (Triticum aestivum L.)

Purpose Kernel texture plays a principal role in determining technological flour properties and end-use quality of wheat products. Hence, a multi-year mutation induction programme was conducted to isolate advanced wheat mutant lines with agro-morphologically superior performance, higher disease resistance and harder grain texture. Materials and methods Radiation mutagenesis was employed in soft textured wheat variety HPW 89 using gamma rays dose of 250, 300 and 350 Gy (Co60: BARC, Mumbai) and evaluated across M1–5 generations. Promising superior mutants selected were evaluated during M4 and M5 generation for induced variability and trait association for agro-morphological and quality traits. The screened mutants were also determined for induced changes at genetic level using gene specific markers for puroindoline genes. Results A total of 293 agro-morphologically superior mutants isolated showed significant genetic variation in the M4 generation. Single kernel characterization system categorized 267 mutants (8.79–50.06) with higher grain hardness than the HPW 89 variety (7.39). Among these, 108 mutants were selected for agro-morphological and molecular characterization. Significant variations were found in these mutants in either pina and pinb or both puroindoline genes. Clustering among these mutants led to the formation of five clusters and a total of eleven mutants were found with better set of agro-morphological, disease resistance and quality traits. Conclusion These mutants can serve as important genetic resource for developing harder texture bread wheat varieties in the future grain quality improvement programmes. These mutants will also bridge the need of bakers and millers’ requirement of varieties with specific texture and quality.

Preparation, characterization, and study of radiation shielding performance of YBa2Cu3Oy/WO3 ceramics exposed to different gamma rays doses

The need for shielding materials amplified gradually due to the usage of ionizing radiation in our lives. The current work studied the impact of different doses of gamma rays on the radiation shielding, optical, and structural properties of the YBCO/WO3 sample. The lattice parameters showed a slight variation when YBCO/WO3 products were exposed to different doses of gamma rays. The density slightly decreased from 6.377 for 0 kGy to 6.369 g/cm3 for 50 kGy. The variation in radiation shielding properties was assessed before and after irradiation based on linear attenuation coefficient (LAC). A slight variation was noted in LAC, so the other parameters were calculated for the non-irradiated sample. According to the results, it might be said that YBCO/WO3 product showed a high stability against gamma-rays’ doses, which can be nominated for exploitation in radiation shielding fields.

OPTIMIZATION OF MUTAGEN DOSE FOR EARLY SEEDLING YIELD ASSOCIATED QUANTITATIVE CHARACTERS IN CANOLA GERM PLASM

Pakistan is facing vital shortage in supply of health promoting crops used as vegetable as well as oil source. Brassica napus (Canola) is such a rich crop that occupy an important position in the oilseed crops of world but unfortunately in Pakistan its germ plasm needs genetic improvement due to narrow gene pool. Mutagenesis is a powerful tool for introducing new alleles, therefore different doses of Gamma radiations (750Gy, 1000Gy, 1250Gy) and EMS (0.5%, 1.0% and 1.5%) were used to induce genetic diversity for early yield associated quantitative characters in two Canola genotypes (Hyola-41 and Shiralee). The data was recorded for days to flowering, days to maturity, plant height, number of branches per plant and pod length in M2 and M3 generation. Significant (p≤0.05) variations were observed for most of phenotypic parameters for both mutagens. The mean value of all the phenotypic parameters in M2 generation increased with moderate dose of EMS and Gamma rays in both genotypes as compared to respective controls. whereas in M3 generation both genotypes were more responsive to Gamma rays (1000 Gy) for all studied characters except early maturity in both tested genotypes. Heritability analysis exhibited high heritability and high genetic advance for most of the traits by moderate and highest does of Gamma rays, while reverse is true for EMS in both generations for Shiralee.

Lumped Parameter Analysis of Autogenous Propellant Pressurization System for Nuclear Thermal Rocket

This work proposes a new propellant management configuration for an ammonia-fueled nuclear thermal propulsion system. The suggested configuration maximizes the advantage deriving from the autogenous pressurization of ammonia by exploiting the thermal power lost by the nuclear reactor toward the vacuum space due to leaking radiation. In this layout, a tank containing ammonia in saturated conditions is placed near the nuclear reactor and receives an input thermal power proportional to the dose of gamma rays and neutrons absorbed by the tank walls and the ammonia itself. Such a thermal power accelerates the vaporization process of the saturated ammonia, thus increasing the pressure in the tank. A pressure regulator valve exploits this overpressure to pressurize the ammonia propellant contained in a run tank to the level required by the mission by connecting the two ammonia volumes. The pressure achieved inside the run tank pushes the propellant with an adequate mass flow rate inside the nuclear reactor. The developed lumped parameter analysis shows how this propellant management system can provide a constant mass flow to the nuclear reactor without using a turbopump assembly. Moreover, it is shown how the proposed concept allows for a reduction in the radiation shield mass.

Improvement of antibacterial activity of AgNPs@PVA-PVP ternary nanocomposite films followed by gamma-ray irradiation treatment for biomedical applications

Our study investigates the influence of several doses of gamma rays on the antibacterial behavior of nanocomposite of silver nanoparticles (AgNPs) doped in a blend of poly (vinyl alcohol) (PVA)-Polyvinyl Pyrrolidone (PVP). AgNPs@PVA-PVP nanocomposite films were fabricated via laser ablation route, and then the synthesized films were subjected to various gamma ray's doses. X-ray diffraction (XRD) data shows a diffraction peak at 2θ = 38° assigned to the existence of AgNPs. Ultraviolet–visible (UV–Vis) results confirm the characteristic peak of silver nanoparticles at 425 nm. The cell viability and antibacterial behavior results confirmed the enhancement in the performance of AgNPs@PVA-PVP composite after irradiated to gamma rays. These values of cell viability have been raised by increasing the dose of gamma rays to 94.5 ± 6.5 % for dose at 70 kGy gamma rays. The values of the inhibition zone of microorganisms were enhanced by raising the doses of gamma rays to 19.5 ± 0.5 and 21.3 ± 0.6 against E. coli and S. aureus respectively specifically for nanocomposite with gamma dose 70 kGy. Thus, the improved antibacterial activity of AgNPs@PVA-PVP nanocomposite could be used in biomedical applications.

Dose mapping of a 252Cf based non-destructive on-line elemental analysis device using Monte Carlo simulation and verification with experimental results

Environmental dose values are critical for human health in non-destructive analysis systems. The International Commission on Radiological Protection (ICRP) has defined specific limitations for permissible dose values. This study assesses the dose distribution of a prototype analysis device utilizing Californium-252 (252Cf) radioactive material through both Monte Carlo simulations and laboratory experiments. Conducted at the Istanbul Technical University (ITU) Energy Institute, the experimental investigation measured gamma-ray and neutron dose values surrounding the device. The objective was to compare dose maps generated by the Monte Carlo-based GEANT4 code with experimental data. Ten dose maps were created, and fundamental statistical analyses were performed. Results indicate that the dose values on the operator’s working surface of the prototype device are within ICRP limits, not exceeding 1 millisievert (mSv) annually, with an effective dose rate of 0.949±0.052 mSv per year. These findings underscore the device’s potential for safe and effective use in radiation safety and environmental dose assessment.

Effects of acute gamma irradiation on the morphology of Curcuma alismatifolia ‘Siam Shadow’ and C. alismatifolia ‘Siam Scarlet’

Curcuma alismatifolia (Pathumma in Thailand), also known as Siam Tulip, is an economically valuable cut flower in Thailand and is continuously bred to enhance its captivating array of colors. This study aimed to investigate the effects of acute gamma irradiation on 2 cultivars of C. alismatifolia ‘Siam Shadow’ and C. alismatifolia ‘Siam Scarlet’ for in vitro propagation and determination of the lethal dose 50 (LD50). The irradiation was conducted at doses of 0, 20, 30, 40 and 80 Gy using young shoots tissue culture. The results of the experiment revealed that the LD50 for ‘Siam Shadow’ could not be determined while ‘Siam Scarlet’ was 29.06 Gy. ‘Siam Shadow’ exhibited chimera leaf formation when exposed to a gamma irradiated dose of 40 Gy, while ‘Siam Scarlet’ exhibited chimera leaf formation when gamma irradiated at doses of 20 Gy in vitro. After planting in a greenhouse, ‘Siam Shadow’ was irradiated with 30 Gy of gamma irradiated, had reduced bract numbers and the bracts became smaller. Conversely, ‘Siam Scarlet’ irradiated with 20 Gy of gamma irradiated, produced smaller inflorescences with only 2 bracts, while irradiation with 30 Gy resulted in 2 lobes and spotted leaves at the edges of the leaves. The results indicated that both the C. alismatifolia cultivars and gamma irradiation rates had a significant influence on survival rate, number of shoots, plant height, chlorophyll mutants and morphology. As the outcome, it was determined that gamma irradiation with optimum doses of gamma rays could induce new characteristics in C. alismatifolia.

Estimation of gamma radiation effects on the polypropylene (PP) films at various doses

Medical polypropylene (PP) is exposed to ionizing radiation during the sterilization process before its use for medical purposes. Herein, the raw and manufactured PP were prepared as polymeric films with the same specifications. Then, raw and manufactured PP were irradiated by different doses of gamma rays in the air to discover the gamma radiation effects on the prepared films. The gamma doses were 25, 50, 75, 100, and 200 kGy. The irradiated raw and manufactured PP were characterized by different analytical techniques such as Electron Spin Resonance Spectrometer (ESR), Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Thermogravimetric Analysis (TGA), Differential Scanning Calorimetry (DSC), Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDX), and mechanical properties. The FTIR study indicated the presence of a new band at a low gamma dose for both cases. The crystallinity percentages of both raw and manufactured PP gradually reduced when the gamma doses increased. The thermal stability of films decreased by increasing the gamma doses. Raising the gamma dosages resulted in a decrease in the mechanical characteristics of raw PP and manufactured PP such as elongation @ break, elastic modulus, and tensile strength.

Effective rare-earth dielectric addition and gamma ray irradiation for achieving highly efficient PDMS triboelectric nanogenerator

This research aims to develop the flexible triboelectric nanogenerator (TENG) using PDMS polymer as the main tribo-material and composite with dielectric rare earth oxide, lanthanum chromite compound; LaCrO3, to enhance generated charge density, before add-on its higher electrical output with gamma ray irradiation. Upon study the effect of different amounts of the loaded LaCrO3 and various doses of gamma ray, the optimum condition can be obtained. The PDMS/LaCrO3 at 5 wt% can reach open-circuit voltage (VOC) and shot-circuit current (ISC) at 89 V and 448 μA. The impressive results of output further appear by additional irradiated gamma radiation. At 150 kGy dose of Irradiated gamma ray, VOC and ISC can jump to 161 V and 963 μA. These output signals, especially ISC, can be ∼34 times higher than that of the pristine PDMS. Scientific discussion regarding characterization, operating mechanism, dielectric properties and electrical output is provided. The practical application of being a small power source is also developed. Therefore, this research can show another useful application of gamma ray in the field of materials design for energy harvesting devices. The knowledge provided in this research can also serve as potential guidance in the field of specific TENG utilization.

An efficient and easy-to-use protocol for induction of haploids in cucumber through parthenogenic embryo development

BackgroundCucumber (Cucumis sativus L.) is a model crop to study cell biology, including the development of haploids and doubled haploids in vegetable crops. In plant breeding, haploid and doubled haploids are valuable tools for developing pure homozygous inbred lines and accelerating genetic progress by reducing the time required for breeding cycles. Besides, the haploids are also valuable in genomic studies. We are reporting the induction of haploids in cucumber involving gynoecious and parthenocarpic genotypes for the first time. This study aimed to assess the efficient induction of haploids through pollination with gamma-irradiated pollen in cucumber. The effect of gamma irradiation dose on pollen viability and germination, fruit setting percentage, seed development, and haploid embryo development in cucumber hybrid genotypes were studied in detail. The goal was to utilize this information to produce haploid plants for genomics and transformation works in this model vegetable crop.ResultsPollination was done on six cucumber genotypes using varying doses of gamma rays (100, 200, 300, 400, and 500 Gy). Genotypes, doses of irradiation, and embryo developmental stage influenced the successful generation of in-vitro haploid plants. The optimal timeframe for embryo rescue was found to be 25 to 30 days after pollination. Haploid embryos were effectively induced using irradiated pollen at 400 to 500 Gy doses. Parthenogenetic plantlets were analyzed, and their ploidy level was confirmed through stomatal physiology, cytology (mitosis), and flow cytometry methods.ConclusionThrough parthenogenic embryo development, it is possible to induce a large number of haploids in cucumber. This technique’s power lies in its ability to streamline the breeding process, enhance genetic gain, and produce superior cultivars that contribute to sustainable agriculture and food security.