Objective: This study aimed at the synthesis and application of selenium nanoparticles (SeNPs) against biofilm formation by multidrug-resistant (MDR) Staphylococcus aureus isolated from the domestic sewage treatment plant. Materials and Methods: Chemically synthesized SeNPs were characterized using Ultraviolet–visible spectroscopy, X-ray diffraction, and high resolution scanning electron microscopy (HR-SEM).. Bacteria were isolated from domestic sewage water samples and characterized and identified using standard techniques. The drug resistance pattern of the isolates was determined using a disk diffusion assay. Biofilms of this MDR isolate were established (microtiter plate method—colorimetric assay and a slide method). Minimum inhibitory concentrations (MIC) of selenium nanorods (SeNRs) and their effect on biofilm formation were established using a colorimetric method. Results: The HR-SEM analysis of nanomaterials revealed its shape (rod), size (between 85 nm and 275 nm), and purity of the material. The disk diffusion assay attributed MDR status to an isolate that was identified and found to be S. aureus, a pathogenic bacterium isolated from an environmental sample. The MICs of antibiotics against biofilm were found to be at least threefold higher than those against the planktonic state. In the presence of SeNRs, biofilm formation was inhibited. Conclusion: SeNRs synthesized using wet chemical method showed antibacterial activity against MDR S. aureus and inhibited biofilm formation by this organism. These SeNRs can be further developed as an alternate drug lead to combat the challenge posed by the MDR bacteria. The study has a future prospectus in investigating the mechanism of inhibition of biofilm formation and its action on preformed biofilm by this isolate.
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