Fat In Dogs Research Articles

Safety of white adipose tissue activator cyclophilin A intracellular peptide (Pep19™) in healthy dogs

Oral consumption of Pep19™, a 10 amino acid intracellular peptide, has been associated with weight loss in rodents and humans via induction of nonshivering thermogenesis. This study aimed to test its safety and tolerance in dogs. Eight healthy adult neutered university owned beagles (4 female and 4 male) were individually housed in runs and fed an extruded kibble in a quantity historically associated with weight stability. They were administered Pep19™ (5 mg/dog/day, 0.32 – 0.49 mg/kg/day) as a once daily oral dose for 28 days. Health screening, including physical examination, body weight, body condition score (BCS), complete blood count, chemistry, total thyroxine, thyroid stimulating hormone, and urinalysis were collected at baseline, day 14, and day 28. Faecal score, appetite, and overall animal temperament and condition were assessed daily. At baseline, average age, weight, and BCS were 3.8 ± 0.3 years, 12.68 ± 2.11 kg, and 6.4 ± 0.7/9, respectively. There were no adverse effects and all blood and urine analyses remained normal. At study termination, average weight, and body condition score (BCS) were 12.53 ± 2.01 kg, and 5.6 ± 0.7/9, respectively. Despite no changes in diet or calorie intake, seven of the dogs lost between 0.7% and 3.8% of their body weight (p<0.01); this was associated with a reduction in body condition score (p<0.05). This initial study shows that Pep19™ is safe for dogs. Future clinical research should investigate its utility as a new approach to reduce excess body fat in dogs.

Possibility of using fatty acid profiles for the authentication of beef adulterated with pork, donkey, and dog meat

Detection of meat adulteration is a critical issue in food labeling procedures and a serious concern related to food fraud, authenticity, and religious beliefs. The current work detected and quantified adulteration of raw ground beef with pork, donkey, and dog meat based on fatty acid profiles using GC-MS/MS. The study design incorporated pork, donkey, or dog meat with beef meat; negative and positive controls were used for the different meat species. Results demonstrated several significant differences (p &lt; 0.05) in fatty acid contents between mixed/adulterated meat and pure beef. In addition, higher total saturated fatty acid levels in ground beef (57.91%) compared to dog fat (46.44%), donkey (38.71%), and pork fat (lard) (40.23%). High total unsaturated fatty acids content was observed in donkey (61.92%), pork (59.77%), and dog (53.56%) fats compared to beef fat (42.09%). On the other hand, total unsaturated and monounsaturated fatty acids in beef meat were lower than in pork, donkey, and dog meat. Moreover, the highest trans-fatty acid content was found in pork compared to the other meat types. All incorporated samples correlated positively with pure ground beef concerning the fatty acid profiles. Therefore, alteration of the mixed beef fatty acid profiles was a potential indicator for adulteration since a marked decrease in total saturated fatty acid content and an increase in unsaturated fatty acids was observed in the substituted samples. We concluded that GC-MS/MS-based fatty acid profiling is a promising technique that can be used to detect meat adulteration.

Clinical Evaluation of Weight Loss Diet on Adiposity Markers and Subcutaneous Fat in Obese Dogs

Background: This clinical study was conducted in thirteen apparently healthy overweight/obese dogs during the period 2018-19. The study involved nutritional interventions such as feeding of high protein and low fat diet and increased physical activity for these dogs, so as to assess effect of these interventions on adiposity markers and subcutaneous fat in obese dogs. Methods: The prescribed weight loss diet consisted of high crude protein (%), crude fibre (%) and reduced fat (%) compared to the mean values of pre-weight loss diets. The study was conducted over a span of 12 weeks and various hemato-biochemical parameters, adiposity markers and subcutaneous fat thickness were measured on 0, 30, 60 and 90 days. Result: Dogs included in this study showed reduction of an average of 9% of their body weight. Significant (P less than 0.05) difference was observed in mean values of Body Condition Score at day 60, from day 30 and this difference persisted until day 90, when compared with day 0 of treatment. Serum cholesterol and leptin concentrations were markedly reduced at the end of weight loss period. Decreased thickness of subcutaneous fat by real time ultrasonography was observed from day 0 to day 90 in five anatomical locations i.e. abdomen, mid abdomen, 9 Inter Costal Space, thigh and lumbar.

Using bioelectrical impedance analysis for modeling regression and predicting body fat accumulation in dogs in East Thailand.

Background and Aim:Obesity in dogs leads to several health problems, such as premature death, and contributes to other diseases. Recently, body fat percentage has been considered to represent the body condition of dogs, and bioelectrical impedance analysis (BIA) is the most effective method for accurately measuring body fat in dogs. In Thailand, information on the body condition of dogs is limited, and there is no standard body fat level for Thai or mongrel dogs. This study was designed to evaluate and analyze the body fat percentage in dogs through BIA using a handheld instrument. The results of this study can help enhance the quality of life and health of dogs and aid in setting a standard body fat level for Thai or mongrel dogs.Materials and Methods:The body fat percentage of 340 Thai and mongrel dogs in East Thailand was measured in the standing position, and the body condition score (BCS) (range, 1–5), sex, sterilization status, age, type of diet, and lifestyle were recorded. A linear regression model was developed to compare the variables and the predicted body fat percentage, and multiple linear regressions were used to analyze the factors for body fat increment.Results:The linear regression model used to estimate the percentage of body fat (y) for each BCS (x) was y = 0.84 + 8.36x (R2 = 0.7219; p < 0.0001); the average body fat percentage was 27.52% for all studied dogs; specifically, 24.83% for the Thai Bangkaew, 26.42% for the Thai Ridgeback, and 27.65% for mongrels. The median body fat percentage was significantly higher in female than in male dogs. We found that as age increases, body fat percentage also increases; this increasing trend begins at the age of 5 years. However, increasing the level of activity and decreasing meal frequency leads to an increase in body fat percentage in neutered male dogs.Conclusion:The average body fat percentage of dogs in East Thailand is 27.52% and this value is expected to increase when these dogs reach the age of 5 years. BIA is a valid and effective measurement tool for detecting the body fat percentage in dogs.

Effects of Synbiotic Preparation Containing Lactobacillus gasseri BNR17 on Body Fat in Obese Dogs: A Pilot Study.

Simple SummaryThe present study evaluated the effectiveness of a synbiotic preparation containing L. gasseri BNR17 on body fat in obese dogs. Body weight and the body condition score showed a significant reduction after 10 weeks of synbiotic preparation administration compared with those at baseline (0-week). The subcutaneous fat mass at the level of the third lumbar vertebra and its proportion, compared with the body mass, reduced significantly after 10 weeks of synbiotic preparation administration, compared with those at baseline. Following synbiotic supplementation, microbiome analysis revealed increased microbial diversity, and functional analysis of the microbiota showed an increased abundance of carbohydrate and lipid metabolism. Based on these results, we consider that synbiotic preparation containing L. gasseri BNR17 may play a role in reducing body fat and resolving obesity.Obesity is an important health concern in humans and dogs. It can cause a variety of secondary problems, including low bacterial diversity. Several approaches have been tried to solve this problem; one of them is probiotic supplementation. Lactobacillus gasseri BNR17 is derived from breast milk and has been proven to be effective for obesity in humans. However, there have been no studies using a synbiotic preparation containing L. gasseri BNR17 for obesity management in dogs. Therefore, the present study evaluated the effectiveness of a synbiotic preparation containing L. gasseri BNR17 in reducing body fat in obese dogs. A group of obese dogs were fed a synbiotic preparation for 10 weeks. Obesity variables included body weight, body condition score, subcutaneous fat thickness, subcutaneous fat mass and proportion of the fat mass. In addition, feces collected at 0-week and 10-week time points were analyzed for the intestinal microbiome. Results showed a significant decrease in body weight, body condition score, and subcutaneous fat mass and proportion at the level of the third lumbar vertebra. Diversity and functional analysis of the microbiota in obese dogs showed increased microbial diversity, and increased abundance of metabolism of carbohydrate, and lipid after supplementation with a synbiotic preparation. This study was conducted as a pilot study, and the results demonstrated that a synbiotic preparation containing L. gasseri BNR17 may play a role in reducing body fat and resolving the obesity in dogs.

Using virtual reality to study reading: An eye-tracking investigation of transposed-word effects

Abstract Eye movements were recorded using a virtual reality (VR) set-up as participants made grammatical decisions to sequences of words. Ungrammatical decisions were harder to make to transposed-word sequences (The white was cat big) compared with control sequences where transposing two adjacent words never produces a correct sentence (The black ran dog fat). Crucially, we found significant transposed-word effects independently of the order in which the two critical words were fixated (i.e., was before cat vs. cat before was), thus falsifying a reading out-of-order account of transposed-word effects.

Endogenous fat losses and true and apparent fat digestibility in adult and growing dogs fed diets containing poultry offal fat.

The objective of this study was to evaluate the coefficient of total tract apparent digestibility (CTTAD) of dietary nutrients, endogenous fat loss (EFL), dietary metabolizable energy (ME) content, coefficient of true digestibility (CTD) of fat and faecal parameters of dogs. A total of 16 puppies and 8 adult dogs were distributed according to a double Latin square experimental design. A basal diet was formulated, to which increasing levels (60, 120 and 180g/kg) of poultry offal fat oil were added. EFL was estimated using hyperbolic and linear regression as a function of LW/day and dry matter (DM) intake, respectively, and the CTD of fat was calculated. The CTTAD of acid-hydrolysis ether extract (AHEE) and ME content increased as dietary poultry offal fat level increased both in adult dogs and in puppies (p<.05). The CTTAD of DM and crude protein were higher in adult dogs than in puppies. EFL was estimated by hyperbolic regression as y=98.1094-13.6202/x and y=99.1280-26.1366/x, where y=CTTAD of AHEE (%) and x=AHEE intake/kgBW, for adult dogs and puppies, respectively, which yielded EFL values of 138 and 262mgAHEE per kgBW per day for adult dogs and puppies respectively. The EFL estimated by linear regression was 7.89g and 8.97g/kgDM intake for adult dogs and puppies respectively. The CTD estimated by both methodologies was higher in puppies than in adults (p<.05). Lower faecal DM and score, and higher ammonia production were obtained in puppies than adult dogs (p<.05). The results indicate good fat utilization and that fat digestibility is underestimated, as the CTTAD of AHEE does not take into account EFL, particularly at low-fat inclusion levels and in growing dogs, which have greater EFL than adult dogs.

Analysis of dog fat in beef sausage using FTIR (Fourier Transform Infrared) combined with chemometrics

Sausage is ready to eat meals for children and adults. The meat contained in a beef sausage might not be consistent to the one listed on the label. FTIR (Fourier Transform Infrared) spectroscopy combined with a chemometric method was the most used methods to detect beef fat with rapid and consistent results. Results of analysis can classify fatty acid composition. The aim of this study was to know the profile of spectra and grouping of beef tallow and dog meat with FTIR. The study was designed by making 7 different variations of dog reference sample concentrations (100%, 75%, 65%, 50%, 35%, 25%, and 100%) beef fat and five other samples were gathered from various street vendors. Results were analyzed using FTIR spectroscopy combined with chemometric with PLS (Partial Least Square) and PCA (Principal Component Analysis). The results of analysis will be analysed using Horizon MB application to obtained optimal wave number at 688-1124 cm - ¹. Results equation obtained y = 0.9999 x + 0.0004 and value R² equal to 0.9999; RMSEC ( root mean square error of calibration ) of 0.30%; RMSEP ( root mean square error of prediction ) of 0.05% and RMSECV ( root mean square error of cross validation ) of 0.05%. The low values of RMSEP, RMSEC, RMSECV and R² close to one indicate that the FTIR combined with chemometric is an appropriate method for analyzing the presence of fat content in the sample. From the analysis showed that 1 of 5 sausage market samples has similarities with dog fat.

Evaluation of visceral fat mass in dogs by computed tomography.

In human medicine, computed tomography (CT) is the gold standard for visceral fat measurement. Research shows that the visceral fat area (VFA) of the umbilical slice is significantly correlated with the visceral fat volume (VFV). In veterinary medicine, however, few studies have evaluated visceral fat using CT. This study aimed to evaluate the visceral fat in dogs using CT images, and determine if the slice significantly correlated with VFV to simplify visceral fat measurements. This retrospective study includes data on 90 dogs that underwent whole-body CT scans for diagnostic purposes. VFV was calculated as the product of VFA and thickness in each CT slice; the correlation between VFV and VFA was analyzed at the level of each lumbar vertebra. Visceral fat percentage (VF%) was calculated as the ratio of the product of VFV and fat density to the body weight. Visceral fat area percentage (VFA%) was calculated as the ratio of VFA to the body area, and its correlation with the VF% and the body condition score (BCS) was analyzed. VFA was highly correlated with VFV at the level of each lumbar vertebra, with the highest correlation (r=0.964) at the L3 level. VFA% was significantly correlated with VF% (r=0.930) and weakly correlated with BCS (r=0.523). This study demonstrates that it is sufficient to use only the L3 slice for visceral fat evaluation and that the evaluation can be based on VFA% of the L3 level.

Authentication of Dog Fat With Gas Chromatography-Mass Spectroscopy Combined With Chemometrics

Indonesia is a country with a majority of Muslim population. As a Muslim majority country, providing halal products becomes a liability. The problem that has received a lot of attention right now is the concern that contamination of food products by meat, one of them is dog meat. The purpose of this study was to authenticate dog fat by using Gas Chromatography-Mass Spectrophotometry (GC-MS)combined with chemometrics. &#x0D; &#x0D; Dogs that used in the study were taken from Bantul, Yogyakarta. Dog snacks were heated in an oven at 90-100ᵒC for approximately one hour. Oils / fats obtained from derivatization process was carried out by using NaOCH3 and BF3. The methyl ester compound was injected into the GC-MS instrument system.&#x0D; &#x0D; The Results of this study was dog fat that analyzed by GC-MS contains 9 types of fatty acids, namely: lauric (1.19 &amp;plusmn; 0.25)%, myristate (4.33 &amp;plusmn; 0.30)%, pentadekanoate (0.12 &amp;plusmn; 0.02)%, palmitoleate (4.60 &amp;plusmn; 0.07)%, palmitate (12.80 &amp;plusmn; 2.90)%, margarate (0.13 &amp;plusmn; 0.09)%, oleate (44 , 33 &amp;plusmn; 5.22)%, stearic (14.71 &amp;plusmn; 0.32)%, and arachidonic (1.29 &amp;plusmn; 0.11)%. Total content of fatty acids in dogs was 50.22% and saturated fatty acids were 33.03%. Chemometric grouping with the Principles Component Analysis (PCA) shows that dog fat is very close to lard. The fatty acids that contained in dog fat can be used to authenticate dog meat.

Apparent total-tract macronutrient digestibility, serum chemistry, urinalysis, and fecal characteristics, metabolites and microbiota of adult dogs fed extruded, mildly cooked, and raw diets1.

Despite their popularity, little research has been performed on lightly cooked and raw diet formats for pets. Therefore, the objective of this study was to determine the apparent total-tract macronutrient digestibility (ATTD); fecal characteristics, metabolites, and microbiota; serum chemistry metabolites; urinalysis; and voluntary physical activity levels of adult dogs fed commercial diets differing in processing type. The diets included: 1) extruded dry kibble (EXT) diet; 2) high-moisture roasted refrigerated (RR) diet; 3) high-moisture grain-free roasted refrigerated (GFRR) diet; and 4) raw (RAW) diet. Eight dogs (mean age = 3.6; mean BW = 13.0 kg) were used in a replicated 4 × 4 Latin square design. Each period consisted of 28 d, with a 14-d adaptation phase followed by a 7-d phase for measuring voluntary physical activity, 1-d adaptation phase to metabolic cages, 5-d phase for fecal and urine collection, and 1 d for blood collection. Except for microbiota, all data were analyzed statistically by mixed models using SAS. Microbiota data were analyzed using Quantitative Insights Into Microbial Ecology (QIIME) and Statistical Analyses of Metagenomic Profiles (STAMP) software. Many differences in digestibility were observed, including greater (P < 0.05) ATTD of CP and fat in dogs fed GFRR and RR than dogs fed EXT. Dogs fed RAW had the lowest fecal pH and DM %, but fecal scores were not affected. Dogs fed RR had higher (P < 0.05) fecal indole and total phenol and indole concentrations than dogs fed the other diets. Dogs fed RAW had a higher (P < 0.05) fecal ammonia concentration than dogs fed the other diets. Fecal microbial diversity was altered by diet, with dogs fed GFRR and RAW having reduced species richness than dogs fed EXT. Dogs fed RR, GFRR, or RAW had lower (P < 0.05) Actinobacteria and higher (P < 0.05) Fusobacteria than dogs fed EXT. Dogs fed RAW or GFRR had higher (P < 0.05) Proteobacteria than dogs fed EXT or RR. Dogs fed RAW had higher (P < 0.05) Bacteroidetes and lower (P < 0.05) Firmicutes than dogs fed EXT. Serum triglycerides were within reference ranges, but greater (P < 0.05) in dogs fed EXT than dogs fed GFRR and RAW. All diets were well tolerated and dogs remained healthy throughout the study. In conclusion, the lightly cooked and raw diets tested were highly palatable, highly digestible, reduced blood triglycerides, maintained fecal quality and serum chemistry, and modified the fecal microbial community of healthy adult dogs.

The potential use of infrared spectroscopy and multivariate analysis for differentiation of beef meatball from dog meat for Halal authentication analysis

Objective: The objective of this research was to assess the suitability of FTIR spectroscopy coupled with multivariate analysis of partial least square regression (PLSR) along with pattern recognition technique of principal component analysis (PCA) for rapid quantitative and qualitative (identification) analysis of dog meat in beef meatball formulation. Materials and Methods: The lipid fraction of meatball was obtained by employing two different extraction techniques, namely Bligh-Dyer and Folch method. FTIR spectral bands correlated with beef fat, pork fat, chicken fat and rat fat were measured, interpreted, and qualitatively analyzed. The small variations among spectra were exploited as a basis tools to differentiate between dog fat and other animal fats. Results : PCA at combined wavenumber regions of 1700-700 cm -1 was capable of identifying dog meat in meatball. These wavenumbers were also used for quantitative analysis of dog meat in meatball using PLSR model. Based on statistical parameters used, namely R 2 , RMSEC and RMSEP, Folch extraction method offered higher R 2 and lower RMSEC and RMSEP than Bligh-Dyer. PCA is succesfully applied for classification between meatball containing dog meat and other meats. Conclusion: FTIR spectroscopy coupled with multivariate analyses of PLSR and PCA was effective means for rapid screening of dog meat in meatball products. http://doi.org/10.5455/javar.2018.e281

Endogenous fat loss and true total tract digestibility of poultry fat in adult dogs.

Fat plays an important role in the carnivore diet. It has high digestibility and high energy content and likely improves digestibility of the diet when present in greater amounts, suggesting that endogenous fat adversely affects the apparent digestibility of fat in low-fat diets. This study aimed to determine the endogenous fat loss (EFL) and true total tract digestibility (TTTD) of fat in dogs by using diets having different fat:carbohydrate ratios. Furthermore, the apparent total tract digestibility (ATTD) of macronutrients and energy, food intake, fecal output, and stool quality were evaluated. Twelve adult Beagle dogs were stratified into three 10-d periods in a 6 × 3 balanced incomplete Latin square design. Dietary treatments included a low-fat basal diet (3.4% fat) supplemented with 6 poultry fat levels (1, 4, 10, 12, 16, or 20%). The dogs were individually housed in metabolic cages and fed to meet the maintenance energy requirements. The ME intake was similar across the diets, despite the clearly low palatability of the 1 and 4% fat diets. Dietary ATTD of DM, OM, fat, and GE were improved after fat inclusion ( < 0.001). The ATTD of CP was not affected by dietary fat increase ( > 0.05). The amount of fecal output decreased with increasing level of fat in the diet, but the fecal score was not affected. The stool quality was close to ideal (2.4-2.8) and no different among diets. The EFL was estimated using 2 regression methods and was expressed as 155 mg∙kg BW∙d or 7.9 g/kg of DMI. The TTTD of poultry fat was the same irrespective of the dietary fat level. Poultry fat was 99.3% digestible for the dogs. The relation between EFL and fat intake was higher for the lower-fat diets, explaining the ATTD of fat increase according to the dietary fat inclusion. In conclusion, dietary fat is well digested by dogs irrespective of the level included, close to 100%. The ATTD of fat in low-fat diets is underestimated because the endogenous content is proportionally greater than that in high-fat diets.

Endogenous fat loss and true total tract digestibility of poultry fat in adult dogs

Endogenous fat loss and true total tract digestibility of poultry fat in adult dogs

In vivo assessment of subcutaneous fat in dogs by real-time ultrasonography and image analysis.

BackgroundSystems for estimating body condition score (BCS) are currently used in canine practice to monitor fatness levels. These tools are cheap and easy to use but lack the necessary precision to monitor small changes in body fat, particularly during weight control treatments or in research. The present work aims to study the application of real-time ultrasonography (RTU) together with image analysis in the assessment of subcutaneous fat depots in dogs. Ultrasound images were collected from five anatomical locations (chest, flank, abdomen, thigh and lumbar) from 28 healthy dogs of different breeds and with a body weight (BW) ranging from 5.2 to 33.0 kg. BCS was collected by visual appraisal using a 5-point scale. Subcutaneous fat thickness (SFT) was estimated from RTU images, using the average of three measurements taken in fat deposits located above the muscles represented in each image. Correlations were established between SFT and BW or BCS as well as a classification of BCS-based fatness [overweight (BCS = 4), ideal (BCS = 3) and lean (BCS = 2)].ResultsSFT was found to differ between the five regions considered (P < 0.001). Abdomen and thigh were the areas displaying the widest variation for the different dogs included in the study and also those correlating most with BW, in contrast to the chest, which showed the least variation. Overall, a strong correlation was found between BCS and SFT. The highest correlations were established for the flank, abdomen and lumbar areas. In every anatomical area, a decrease in SFT was observed across all three BCS classes, ranging from 48 to 65 % among overweight and ideal dogs, and from 46 to 83 % among ideal and lean dogs.ConclusionsPreliminary data showed that within this population there was a strong correlation between BCS and SFT estimated from RTU images. It was also observed that RTU measurements for fat thickness differed among the anatomical points surveyed suggesting differences in their sensitivity to a change in BCS. The images displaying the best prediction value for fatness variations were those collected at the lumbar and abdomen areas.

Why is my dog fat? Gene deletion may provide clues

Why is my dog fat? Gene deletion may provide clues

Ultrasound Measurement of Visceral Fat in Obese Dogs: Association with Changes in Serum Lipids and Leptin Concentration

Ultrasound Measurement of Visceral Fat in Obese Dogs: Association with Changes in Serum Lipids and Leptin Concentration Obesity is a common nutritional disorder in dogs and it has adverse effects on health and longevity. In humans, the detrimental health effects of obesity are well known and the regional fat distribution, especially intra-abdominal adiposity, is an important index of cardiovascular and metabolic deterioration. The significance of central obesity has not been well studied in dogs, but there is evidence for the presence of visceral obesity also in dogs. However, a simple and noninvasive method to assess the regional distribution of body fat is not available. The objective of this prospective study was to investigate a possible application of ultrasound to quantify visceral fat and to predict metabolic changes in obese dogs..

A Historical Perspective on Clinical Advances in Pancreatic Diseases

Some of the first glimpses into acute pancreatitis were by Reginald H. Fitz, pathologist, who described the clinical features of acute pancreatitis in 1889 and subtyped it into hemorrhagic, suppurative, and gangrenous pancreatitis.1 However, he believed pancreatitis was the result of gastroduodenitis. In 1896, Chiari put forth the premise that pancreatitis was the result of pancreatic autodigestion.2 Claude Bernard demonstrated that pancreatic secretion was involved in the digestion of protein, carbohydrate, and fat in dogs.

Comparison of a bioimpedance monitor with dual-energy x-ray absorptiometry for noninvasive estimation of percentage body fat in dogs

To assess performance of a portable bioimpedance monitor for measurement of body composition in dogs. 24 client-owned dogs. Percentage body fat was measured via dual-energy x-ray absorptiometry (DEXA) and with a portable bioimpedance monitor, and body condition score (BCS) was measured by use of a 9-integer scale. Although the precision of the bioimpedance monitor was good, this varied among dogs. Body position (standing vs sternal) had no effect on bioimpedance results. There was a significant association between results determined via DEXA and bioimpedance, but this association was weaker than between DEXA and BCS. When agreement was assessed via Bland-Altman plot, the bioimpedance monitor under- and overestimated values at high and low body fat percentages, respectively. In 9 dogs, body fat measurements were taken before and after weight loss to determine the proportional loss of tissue mass during weight management. There was a significant difference in the estimated percentage of weight lost as fat between the DEXA and bioimpedance methods. Although percentage body fat measured by use of a portable bioimpedance monitor correlated well with values determined via DEXA, the imprecision and inaccuracy in dogs with high percentage body fat could make the monitor inappropriate for clinical practice.

QUALITY OF HOT DOG SAUSAGES

The volume of hot dog sausages plays an important role in meat production. Due to their desirable eating properties (softness, juicy, mild taste and smell) they form a group of sausages acceptable for all consumer categories. They are produced according to producer’s specification, which are not the same. Therefore, the differences in quality may be expected. The objective of this paper was to make a comparative analyses of three parameters in five different products present on Novi Sad market: the content of water, fat and protein, and to determine the average content of these parameters in hot dogs produced by different producers. The average content of water ranged from 56.29±3.30% to 61.44±4.08%. The differences between the average content of water in the product of producer A and C were with no statistic significance (p=0.05), and there was no difference between the average water content in other products. The average total fat in hot dogs ranged from 17.60±3.07% to 20.66±4.65%. The average fat content in the products of D and B producer, as well as Cand B, was without statistically significant difference (p=0.05). The average fat content in the products of producer E and D differed p=0.01. In all other comparisons the difference between the average fat content was p=0.001. The average meat protein content ranged from 11.77±1.08% to 15.41±1.82%. Between the products from producer A and C there was no statistical significant difference (p=0.05). The average meat protein content in the products of D and E producers showed statistic difference of p=0.01, but the average content in other producers was p=0.001. The content of meat protein in all hot dogs were within limits of regulations (10%). Statistically significant difference of the average water content in five different producers was discovered in one case (out of ten), the average fat content in two, and the average protein content in one of the examined products.