DNA-damaging Compounds Research Articles

Mutagenicity testing with TA97 and TA102 of 30 DNA-damaging compounds, negative with other Salmonella strains

In a comparative study on 135 compounds of various chemical classes, 30 agents inducing direct nonreparable DNA damage in repair-deficient E. coli failed in reverting strains TA1535, TA1537, TA1538, TA98 and TA100 of S. typhimurium (De Flora et al., 1984b). These compounds were re-assayed in the Ames test using strains TA97 and TA102. A dose-dependent mutagenic response was detected with aminoantipyrine and p-rosaniline in TA97 and with streptomycin and formaldehyde in TA102. p-Rosaniline was the only mutagen requiring metabolic activation. 5 compounds, i.e. o-aminophenol in TA97 and methanol, ethanol, cadmium chloride and cadmium sulfate in TA102, induced a reproducible increase in revertants over controls, but this was less than 2-fold. The remaining 21 chemicals — including amino compounds, aliphatics, aromatics, heterocycles, hydrazine derivatives and inorganics — confirmed their inactivity in the Ames test. Overall data for 135 compounds, comparing the Ames test (7 strains) and the DNA-repair test (3 strains), are re-assessed on the basis of these findings.

Induction of DNA repair synthesis in isolated rat hepatocytes by 5-diazouracil and other DNA damaging compounds

5-Diazouracil, which has been recommended in cancer chemotherapy, induced DNA repair synthesis in isolated rat hepatocytes. Repair synthesis was determined by means of the bromodeoxyuridine (BrdUrd)-CsCl-gradient centrifugation method. The validity of this technique was demonstrated using various DNA damaging compounds.

Induction of DNA-repair synthesis in human lymphoblastoid cells by metabolically activated chemicals as short-term test for DNA-damaging compounds

Induction of DNA-repair synthesis in human lymphoblastoid cells by metabolically activated chemicals as short-term test for DNA-damaging compounds