Diverse Profiles Research Articles

Microbial profiling, antimicrobial resistance surveillance, and molecular detection of MecA gene in Staphylococcal strains from donor human milk: Insights from a milk bank investigation

PurposeDonor human milk (DHM) from milk banks provides vital nutrition to vulnerable infants. Understanding its microbial profile and antimicrobial resistance patterns is crucial for ensuring its safety and efficacy. This study aimed to profile the microbial composition, detect antibiotic resistance, and identify the presence of mecA gene in Staphylococcal strains from DHM samples. Materials and methodA total of 151 DHM samples were collected from a regional human milk bank in North India. Microbial identification was performed using MALDI TOF MS, and antimicrobial susceptibility testing was conducted using the disc diffusion method. Molecular methods, including PCR, were employed for mecA gene detection. ResultsThe study revealed a diverse microbial profile, with Staphylococcus species being predominant. Acinetobacter and Pseudomonas species were also prevalent, raising concerns due to their association with healthcare-associated outbreaks. High rates of antibiotic resistance were observed across both Gram-positive and Gram-negative bacteria, with resistance to commonly used antibiotics such as penicillin, clindamycin, erythromycin, and ceftriaxone. The mecA gene, associated with methicillin resistance, was detected in a significant proportion of Staphylococcal isolates. ConclusionThe study underscores the importance of rigorous microbial analysis and antimicrobial susceptibility testing in assessing the safety of DHM. The presence of diverse microbial species, including antibiotic-resistant strains and the mecA gene in Staphylococcal strains, emphasizes the need for stringent hygiene practices and continuous surveillance in milk banks. Implementing comprehensive screening protocols and adhering to best practices in milk handling and pasteurization are crucial for safeguarding the health of vulnerable infants reliant on donor milk.

Exploring the Genetic Diversity of Mycoplasma hyopneumoniae in Pigs with Pneumonia and Pleurisy at Slaughter.

Mycoplasma (M.) hyopneumoniae is the key pathogen of the porcine respiratory disease complex (PRDC) and contributes to pleurisy in pigs. Due to its limited metabolism and laborious cultivation, molecular tools are useful for diagnosis. This study investigated the genetic diversity of M. hyopneumoniae in slaughter pigs with pneumonia and pleurisy, and it assessed co-infections by Pasteurella multocida type A (PM), Actinobacillus pleuropneumoniae (APP), and swine influenza virus A (sIVA). Lungs (n = 70) with different pleurisy scores and lesions compatible with M. hyopneumoniae infection were collected for convenience. Macroscopic and microscopic evaluations were performed. M. hyopneumoniae was detected using qPCR, and MLST was used for genetic characterization. Co-infections with PM and APP were also evaluated by qPCR, while the immunohistochemistry assessed sIVA infection. All lungs were positive for M. hyopneumoniae. Histopathology confirmed M. hyopneumoniae-associated lesions. MLST characterization was possible in 25 lungs and revealed 10 distinct allelic profiles, with none matching known sequence types in the public database. Co-infections were detected in 40% of the samples with APP and 32% with PM, with 12% showing both pathogens and 52% of the samples presenting microscopic lesions compatible with sIVA infection. The diverse genetic profiles found underscore the need for research on isolation and potential pathogenic variations.

A novel whole blood assay to quantify the release of T cell associated cytokines in response to Bordetella pertussis antigens

BackgroundBordetella pertussis continues to cause whooping cough globally even in countries with high immunisation coverage. Booster vaccinations with acellular pertussis vaccines are thus used in children, adolescents, and adults. T cell immunity is crucial for orchestrating the immune response after vaccination. However, T cell assays can be expensive and difficult to implement in large clinical trials. In this study, a whole blood (WB) stimulation assay was developed to identify secreted T cell associated cytokines in different age groups after acellular pertussis booster vaccination. Material and methodsLongitudinal WB samples were collected from a small set of subjects (n = 38) aged 7–70 years participating in a larger ongoing clinical trial. For assay development, samples were diluted and incubated with purified inactivated pertussis toxin (PT), filamentous haemagglutinin (FHA), inactivated B. pertussis lysate, and complete medium (M) as stimulating conditions, with anti-CD28 and anti-CD49d as co-stimulants. Different timepoints around the vaccination (D0, D7, D14, D28), WB dilution factor (1:2, 1:4) and incubation time (24 h, 48 h, 72 h) were compared. Responses to 15 cytokines were tested with Luminex/multiplex immunoassay. ResultsThe optimized assay consisted of WB incubation with M, PT, and FHA (including the two co-stimulants). After 48 h incubation, supernatants were collected for measurement of seven selected T cell associated cytokines (IL-2, IL-5, IL-10, IL-13, IL-17 A, IL-17F, and IFN-y) from samples before and 28 days after vaccination. PT stimulation showed a trend for upregulation of IL-2, IL-13, and IL-17 A/F for adult subjects, whereas the responses of all cytokines were downregulated for the paediatric subjects. Furthermore, PT and FHA-stimulated WB showed diverse cytokine producing profiles. ConclusionsThe developed WB-based cytokine assay was shown to be less costly, easy to perform, and functional in differently aged individuals. Further, it requires only a small amount of fresh blood, which is beneficial especially for studies including infants. Our results support the use of this assay for other immunological studies in the future.

The OBS UK Dashboard: an interactive tool for representative trial site selection to facilitate equality and diversity in maternity research

BackgroundObstetric Bleeding Study UK (OBS UK) (award ID: 152057) is a National Institute for Health and Care Research (NIHR)-funded stepped wedge cluster randomised controlled trial of a complex intervention for postpartum haemorrhage. This was developed in Wales and evaluated in a feasibility study, with improvements in maternal outcomes observed. Generalisability of the findings is limited by lack of control data and limited ethnic diversity in the Welsh obstetric patient population compared to the United Kingdom (UK): 94% of the Welsh population identifies as White, versus 82% in the UK. Non-White ethnicity and socioeconomic deprivation are linked to increased risk of adverse maternal outcomes. Traditionally, decisions regarding site selection are based on desire to complete trials on target in ‘tried and tested’ research active institutions. To ensure widespread applicability of the results and investigate the impact of ethnicity and social deprivation on trial outcomes, maternity units were recruited that represent the ethnic diversity and social deprivation profiles of the UK.MethodUsing routinely collected, publicly available data, an interactive dashboard was developed that demonstrates the demographics of the population served by each maternity unit in the UK, to inform site recruitment. Data on births per year, ethnic and socioeconomic group of the population for each maternity unit, across the UK, were integrated into the dashboard.ResultsThe dashboard demonstrates that OBS UK trial sites reflect the ethnic and socioeconomic diversity of the UK (study vs UK population ethnicity: White 79.2% vs 81.7%, Asian 10.5% vs 9.3%, Black 4.7% vs 4.0%, Mixed 2.5% vs 2.9%, Other 3.0% vs 2.1%) with variation in site demography, size and location. Missing data varied across sites and nations and is presented.ConclusionThe NIHR equality, diversity and inclusion strategy states studies must widen participation, facilitating individuals from all backgrounds to engage. The development of this novel interactive dashboard demonstrates an innovative way of achieving this. National Health Service (NHS) maternity researchers should consider using this tool to enhance diversity in research, address health disparities and improve generalisability of findings. This approach could be applied to healthcare settings beyond maternity care and across different global populations.Trial registrationISRCTN 17679951. Registered on August 30, 2023.

Finally Freed-Cannabis in South Africa: A Review Contextualised within Global History, Diversity, and Chemical Profiles.

Cannabis sativa L. is a monotypic genus belonging to the family Cannabaceae. It is one of the oldest species cultivated by humans, believed to have originated in Central Asia. In pivotal judgements in 2016 and 2018, the South African Constitutional Court legalised the use of Cannabis within the country for medicinal and recreational purposes, respectively. These decrees opened opportunities for in-depth research where previously there had been varying sentiments for research to be conducted on the plant. This review seeks to examine the history, genetic diversity, and chemical profile of Cannabis. The cultivation of Cannabis by indigenous people of southern Africa dates back to the eighteenth century. Indigenous rural communities have been supporting their livelihoods through Cannabis farming even before its legalisation. However, there are limited studies on the plant's diversity, both morphologically and genetically, and its chemical composition. Also, there is a lack of proper documentation of Cannabis varieties in southern Africa. Currently, the National Centre for Biotechnology Information (NCBI) has 15 genome assemblies of Cannabis obtained from hemp and drug cultivars; however, none of these are representatives of African samples. More studies are needed to explore the species' knowledge gaps on genetic diversity and chemical profiles to develop the Cannabis sector in southern Africa.

Diverse soliton wave profile assessment to the fractional order nonlinear Landau-Ginzburg-Higgs and coupled Boussinesq-Burger equations

The space–time fractional Landau-Ginzburg-Higgs equation and coupled Boussinesq-Burger equation describe the behavior of nonlinear waves in the tropical and mid-latitude troposphere, exhibiting weak scattering, extended connections, arising from the interactions between equatorial and mid-latitude Rossby waves, fluid flow in dynamic systems, and depicting wave propagation in shallow water. The improved Bernoulli sub-equation function method has been used to achieve new and wide-ranging closed-form solitary wave solutions to the mentioned nonlinear fractional partial differential equations through beta-derivative. A wave transformation is applied to renovate the fractional-order equation into an ordinary differential equation. Some standard wave shapes of multiple soliton type, single soliton, kink shape, double soliton shape type, triple soliton shape, anti-kink shape, and other types of solitons have been established. The more updated software Python is used to display the solutions by using 3D and contour plotlines to describe the physical significances of attained solutions more clearly. The findings of this study are straightforward, adaptable, and quicker to simulate. It has been notable that the improved Bernoulli sub-equation function method is practical, effective, and offers more sophisticated solutions that can help togenerate a large number of wave solutions for various models.

Assessment of some parameters of the chemical composition of Thymus kotschyanus Boiss. Growing in different ecological zones of the Republic of Armenia

Background: Thymus kotschyanus Boiss., commonly known as thyme, is a small semi-shrub valued for its aromatic and medicinal properties. Found throughout the Middle East and the Caucasus, it adapts to various ecological conditions and is rich in biologically active compounds, including essential oils, flavonoids, and phenolic acids. Its diverse chemical profile has made it a focus of pharmacological research for its antimicrobial, antioxidant, and anti-inflammatory effects, highlighting its potential therapeutic applications. Objective: The study aimed to investigate the bioactive compounds in wild T. kotschyanus Boiss. plants grown under diverse ecological conditions, with an emphasis on how these compounds change in response to varying environmental contexts. Materials and Methods: The chemical composition of Thymus kotschyanus Boiss. was investigated during its flowering phenophase. Aerial parts of plants were collected from the Tavush (40°880518N, 45°427958E) and Kotayk (40°17’56"N, 44°33’15"E) regions in Armenia between 2021 and 2023. The study focused on analyzing ash and moisture content, phytochemical screening, and mineral content. Results: The ethanolic extracts of Thymus kotschyanus Boiss from both regions contained a diverse array of bioactive compounds, including coumarins, flavonoids, alkaloids, tannins, phenols, carbohydrates, proteins, and saponins. Plants from the Tavush region exhibited a moisture content 1.83% higher than those from the Kotayk region, reflecting a 36.1% increase. Similarly, the ash content was 1.3% higher in Tavush plants, resulting in a 17.1% increase compared to Kotayk plants. Analysis of macro- and microminerals revealed that potassium was the most abundant macromineral, followed by calcium, magnesium, phosphorus, and sodium. Among the microminerals, iron had the highest concentration, followed by zinc and copper. Conclusion: The study demonstrated significant differences in the biologically active compounds, mineral composition, moisture, and ash content of T. kotschyanus Boiss. from different ecological zones in Armenia. Plants from the Tavush region exhibited higher moisture and ash content, as well as elevated levels of vitamins A and C, phenolic content, and essential minerals such as calcium, magnesium, sodium, potassium, and phosphorus, compared to those from the Kotayk region. These findings underscore the impact of environmental conditions on the quality and composition of T. kotschyanus Boiss., highlighting its potential as a valuable source of biologically active compounds and essential minerals. Keywords: aromatic plants, bioactive compounds, essential minerals, wild thyme, T. kotschyanus Boiss

Comprehensive Analysis of Garcin Syndrome: A Systematic Review of the Etiology, Diagnosis, and Treatment.

Garcin syndrome is a rare neurological condition characterized by progressive unilateral involvement of multiple cranial nerves, without typical intracranial hypertension. It is often linked with aggressive malignancies and invasive infections; hence, it presents significant diagnostic and therapeutic challenges. Despite the advances in medical technology, the prognosis still remains poor, and there is limited literature on comprehensive reviews regarding its etiology, diagnosis, and management. A Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA)-based systematic review was performed in order to compile updated evidence related to Garcin syndrome. The PubMed and Scopus databases were comprehensively searched using the search terms "Garcin syndrome" AND ("etiology" OR "diagnosis" OR "treatment" OR "management"). Information was obtained from case reports and focused on common etiologies, clinical presentations, diagnostic methodologies, treatment protocols, and outcomes. The review discussed very diverse etiologies of Garcin syndrome. The most common among these were skull base tumors, metastatic lesions, and invasive infections like mucormycosis. Most of these were multiple cranial nerve (CN) involvements in which CN V (trigeminal nerve), CN VII (facial nerve), or CN XII (hypoglossal nerve) involvement was common. Advanced imaging, especially MRI, played a very crucial role in diagnosis, showing the presence of extensive bony destruction with the involvement of cranial nerves. Treatment was varied according to the etiology, ranging from chemotherapy and radiotherapy in neoplastic cases to active surgical intervention supported by antifungal therapy in infected cases. Garcin syndrome is a clinical challenge due to its diverse etiologies and complex management profile. While early diagnosis and intervention are emphasized, the prognosis remains grave, especially in cases presenting with metastatic disease or immunocompromised states. Future research should focus on better, more sensitive diagnostic modalities and the investigation of newer therapeutic approaches for Garcin syndrome patients.

Simulation study of flexible wavefront shaping in Smith-Purcell radiation with aperiodic metagratings

Smith-Purcell radiation (SPR) is a versatile platform for finely tuning nanoscale light across a broad spectral range. This study introduces a theoretical approach for shaping SPR wavefronts using aperiodic metagratings (AMGs). The AMGs consist of arrays of identical metal nano-rods (MNRs), with each MNR's spatial position precisely adjustable. This precise adjustment allows for effective modulation of the spatial phase distribution of SPR. To demonstrate the efficacy of this method, we conduct simulations to achieve diverse wavefront profiles of focusing, deflection, Bessel beams, and Airy beams. Additionally, our approach allows for integrating multiple SPR wavefront functionalities within a combo AMG. By employing the asymmetric L-shaped meta-atom design, we achieve simultaneous SPR polarization conversion and wavefront shaping. This method is promising for developing highly adaptable and multifunctional nanoscale light sources.

Morphological and Molecular Characterization of Trichoderma Isolates from Vegetable Crop Rhizospheres in Nepal

Background Trichoderma spp. hold significant potential as biocontrol agents in agriculture due to their antagonistic properties against plant pathogens. The study aimed to characterize and identify Trichoderma isolates from rhizospheric regions of vegetable crops. Methods In this study, Trichoderma isolates were collected from rhizospheric soil samples of vegetable crops from different ecological zones and were selected for comprehensive morphological and molecular characterization. The isolates were visually assessed for colony color, growth pattern, aerial mycelium presence, phialide and conidial morphology, and chlamydospore presence. Molecular analysis was employed based on ITS and tef-1α sequences. Diversity indices were also computed for different ecological zones. Results The morphological characteristics and phylogenetic trees for both regions provided a clear species resolution, with four main clades: Harzianum, Viride, Brevicompactum and Longibrachiatum with 12 species T. harzinaum, T. afroharzianum, T. lentiforme, T. inhamatum, T. camerunense, T. azevedoi, T. atroviride, T. asperellum, T. asperelloides, T. koningii, T. longibrachiatum and T. brevicompactum and nine species as a new country record. Diversity indices indicated that high mountain regions displayed the highest species diversity and evenness (H = 1.724 [0.28], J = 0.84, D = 0.28), followed by hilly regions (H = 1.563 [0.28], J = 0.72, D = 0.28). Plains, on the other hand, exhibited lower species diversity (H = 1.515, J = 0.66, D = 0.33). The calculated species abundance values showed that plains (E = 2.11), mid-hills (E = 1.95), and high mountains (E = 1.99) each had their unique diversity profiles. Notably, T. afroharzianum and T. asperellum were predominant. Conclusions Overall, the study unveiled a rich diversity of Trichoderma species in different agricultural zones of Nepal. These findings shed light on the ecological distribution and diversity of Trichoderma spp., which could have significant implications for sustainable agriculture and biological control strategies.

Exploration of Sustainable Development Strategies for Budai Opera in the Eastern Fujian Region

This study explores sustainable development strategies for Budai Opera in the Eastern Fujian region, aiming to preserve and promote this traditional art form amidst modern challenges. Through a mixed-method approach, including qualitative interviews, quantitative surveys, and observational data, diverse dimensions of Budai Opera sustainability are examined. Results reveal a diverse demographic profile of stakeholders, varying levels of awareness and interest in Budai Opera, and preferences for cultural events. Economic viability, environmental impact, community engagement, policy support, audience preferences, and technological innovations are also explored. Key findings emphasize the importance of holistic approaches that balance economic, cultural, environmental, and social considerations in promoting Budai Opera sustainability. Addressing challenges such as declining audiences, economic pressures, environmental impacts, and policy constraints, while capitalizing on opportunities for diversification, innovation, community engagement, and advocacy, are essential for sustaining Budai Opera as a cherished cultural heritage in the Eastern Fujian region.

Unlocking cross-modal interplay of single-cell joint profiling with CellMATE.

A key advantage of single-cell multimodal joint profiling is the modality interplay, which is essential for deciphering the cell fate. However, while current analytical methods can leverage the additive benefits, they fall short to explore the synergistic insights of joint profiling, thereby diminishing the advantage of joint profiling. Here, we introduce CellMATE, a Multi-head Adversarial Training-based Early-integration approach specifically developed for multimodal joint profiling. CellMATE can capture both additive and synergistic benefits inherent in joint profiling through auto-learning of multimodal distributions and simultaneously represents all features into a unified latent space. Through extensive evaluation across diverse joint profiling scenarios, CellMATE demonstrated its superiority in ensuring utility of cross-modal properties, uncovering cellular heterogeneity and plasticity, and delineating differentiation trajectories. CellMATE uniquely unlocks the full potential of joint profiling to elucidate the dynamic nature of cells during critical processes as differentiation, development, and diseases.

A Study of Disease Prognosis in Lung Adenocarcinoma Using Single-Cell Decomposition and Immune Signature Analysis.

Background: The development of tumors is a highly complex process that entails numerous interactions and intricate relationships between the host immune system and cancer cells. It has been demonstrated in studies that the treatment response of patients can be correlated with the tumor microenvironment (TME). Consequently, the examination of diverse immune profiles within the TME can facilitate the elucidation of tumor development and the development of advantageous models for diagnoses and prognoses. Methods: In this study, we utilized a single-cell decomposition method to analyze the relationships between cell proportions and immune signatures in lung adenocarcinoma (LUAD) patients. Results: Our findings indicate that specific immune cell populations and immune signatures are significantly associated with patient prognosis. By identifying poor prognosis signatures (PPS), we reveal the critical role of immune profiles and cellular composition in disease outcomes, emphasizing their diagnostic potential for predicting patient prognosis. Conclusions: This study highlights the importance of immune signatures and cellular composition, which may serve as valuable biomarkers for disease prognosis in LUAD patients.

Comparison and regional suitability evaluation of different backfill source heat pumps using TRNSYS

As global energy demand and carbon emissions continues to rise year by year, the use of renewable energy for heating becomes increasingly urgent. This study employs solar and geothermal energy to supply heat to mining facilities. Initially, three distinct heating systems for mining areas were modeled in TRNSYS: the Backfill Source Heat Pump (BSHP), the Solar Assisted Backfill Source Heat Pump (SABSHP), and the Solar Assisted Backfill Source Heat Pump with Seasonal Heat Storage (SABSHP-SHS). Following a decade of simulation, the optimal system was identified through a comparative analysis that encompassed backfill temperature equilibrium, the inlet and outlet water temperatures for buried pipes, Coefficient of Performance (COP), Partial Load Ratio (PLR), and economic factors. Over the 10-year simulation period, the COP and PLR of the SABSHP-SHS system outperformed both the BSHP and SABSHP systems, showing higher energy efficiency and better heat pump performance. To further assess the regional adaptability of the SABSHP-SHS system, simulations were conducted across four regions with diverse climatic profiles: Shijiazhuang, Yan’an, Xining, and Hegang. The analysis focused on heat collection, efficiency, backfill temperature variation, system energy consumption, and the energy efficiency ratio. The findings indicate that Hegang exhibits the highest solar heat collection efficiency at 89.9% during the non-heating season, while Xining achieves a significantly higher heat collection efficiency of 54.1% during the heating season. These results suggest that the SABSHP-SHS system is particularly effective in regions with pronounced seasonal variations, characterized by extended cold winters and brief, intense summers.

Diverse soliton wave profile analysis in ion-acoustic wave through an improved analytical approach

In engineering and applied sciences, several physical phenomena can be more precisely characterized by employing nonlinear fractional partial differential equations. The primary goal of this research is to examine the traveling wave solution in closed form for the nonlinear acoustic wave propagation model known as the time fractional simplified modified Camassa–Holm equation, which is used to explain the unidirectional propagation of shallow-water waves with non-hydrostatic pressure and explains the dispersion properties of numerous phenomena like fluid flow, control theory, liquid drop patterning in plasma, acoustics, fusion, and fission processes, etc. The utmost potential approach, namely the new auxiliary equation technique, is applied for analyzing the time nonlinear fractional simplified modified Camassa-Holm equation in the logic of the newest established truncated M-fractional derivative. The fractional partial differential equations have been transformed to the ordinary differential equation using the complex wave transformation in the sense of truncated M-fractional derivative. A variety of soliton solutions, including anti-kink, single soliton, anti-bell, bell, kink, multiple soliton, double soliton, singular-kink, compacton shape, periodic shape, and so many, are displayed in the diagram of 3D and contour plots by taking into account a number of various parameters. It is essential to point out that all derived outcomes are directly compared to the original solutions to certify their exactness. Results show that the used scheme is capable, simple, and straightforward and can be useful to a variety of complex phenomena. The acquired results are unique for the model equation and could be applied to the analysis of several nonlinear study fields.

Genome-wide analysis of two repeats containing MYB transcription factors in groundnut identifies drought-inducible genes involved as a negative regulator of root nodulation

The MYB gene superfamily encompasses a group of related genes found in all eukaryotes. In contrast to animals, higher plants contain large numbers of two-repeat MYB genes, which have been established in regulating crucial developmental, biotic, and abiotic stress responses that profoundly affect the plant's yield. However, a comprehensive analysis of the two-repeat MYB gene family in groundnut and its progenitors, especially the role of two-repeat MYB genes in response to drought stress, and the effect of those genes in nodulation have not been reported so far. Our recent analysis of the groundnut genome has identified 79 (Arachis duranensis), 84 (Arachis ipaensis), and 161 (Arachis hypogaea) two-repeat MYB genes, which belong to multiple distinct subgroups based on their architecture. Here, we provided a complete overview of the gene structure, protein motif organization, chromosome localization, gene duplication events, and synteny analysis to clarify evolutionary perspectives. Members of the same subgroup showed highly conserved structure and motif compositions. The whole-genome duplication and segmental duplication likely contributed to the expansion of two-repeat MYB genes in Arachis hypogaea. The upstream sequences of most genes contained phytohormone-responsive, stress-responsive, light-responsive, and plant growth-related elements. The genes not only have diverse expression profiles across different development stages but as shown in our experimental findings, most of them were induced by ABA and drought-related stress. Further gene silencing experiments demonstrated that two drought-inducible MYB genes, homologs of Arabidopsis MYB96 and MYB94, function as a negative regulator of root nodulation. The results of this study can serve as a strong foundation for further elucidation of the physiological and molecular function of two-repeat MYB genes in groundnut.

Understanding wise leadership: Employees’ perspective in Oman’s government sector

Orientation: Success is a common goal in both personal and professional life, and wise leadership is key to achieving it. Effective leadership guides and motivates teams, driving them toward success and excellence.Research purpose: This study examines wise leadership in Oman’s government sector, using Hassi and Storti’s theoretical frameworks. It explores dimensions such as Intellectual Shrewdness, Spurring Action, Moral Conduct, and Cultivating Humility to improve employee awareness and perception.Motivation for the study: To assess wise leadership qualities across different dimensions and demographic groups to identify strengths and areas for improvement in leadership practices.Research approach/design and method: A descriptive method was employed, and a questionnaire was used as a data gathering tool. The data were analysed by several techniques including T-tests, ANOVA and descriptive statistics. Data were anonymously collected from 300 respondents across the Ministry of Labour, Ministry of Information and Taxation Authority, representing diverse demographic profiles and organisational ranks.Main findings: The findings highlight that practical wisdom is vital for organisational resilience, effective communication, and societal well-being in complex governance. While there is broad agreement on key leadership traits, minor discrepancies suggest challenges in applying theoretical ideals, especially concerning vulnerability and humility.Practical/managerial implications: Providing a detailed look at employees’ views on wise leadership and the impact of personal characteristics on these perceptions.Contribution/value-add: Provides a comprehensive view of employees’ perceptions of wise leadership and how personal characteristics influence these perceptions.

Innovative of cascara as potential in beverage, food and their functional impact: a review

SummaryCascara's beverage and food innovation has sparked interest due to its unique flavour profile and potential health benefits. Cascara drink, which comes from dried coffee pulp husks, offers a distinctive aroma reminiscent of cherry, mango and tamarind. Research has highlighted cascara's high antioxidant content, making it a functional and healthy drink choice. Sensory analysis has shown that cascara drinks exhibit dominant citric acid and black tea flavours with a strong sour aroma, appealing to consumers who enjoy coffee and tea. Furthermore, post‐harvest processing significantly influences the chemical composition of cascara beverages, with different processing methods resulting in varying levels of caffeine, total phenolic compounds and antioxidant activity. This innovation not only offers a refreshing drink but also shows the potential of cascara to be a valuable source of antioxidants with diverse flavour profiles and contributing to the global market.

Diverse Clinical and Immunological Profiles in Patients with IPEX Syndrome: a Multicenter Analysis from Turkey.

Immunodysregulation, Polyendocrinopathy, Enteropathy, and X-linked syndrome (IPEX), caused by pathogenic FOXP3 variants, is a rare autoimmune disorder with diverse clinical features, including early-onset diabetes, eczema, and enteropathy. Atypical cases show milder symptoms and unique signs, requiring different treatments. Therefore, there are ambiguities in the accurate diagnosis and management of IPEX. We sought to present clinical, genetic, and immunological assessments of 12 IPEX patients with long-term follow-up to facilitate the diagnosis and management of the disease. Clinical findings and treatment options of the patients were collected over time. Lymphocyte subpopulations, protein expressions, regulatory T (Treg) and circulating T follicular helper (cTFH) cells, and T-cell proliferation were analyzed. Predominant presentations included autoimmunity (91.6%), failure to thrive (66.7%), and eczema (58.3%). There were four classical and eight atypical IPEX individuals. Allergic manifestations were more common in atypical patients. Notably, chronic diarrhea demonstrated heightened severity compared to other manifestations. Four patients (33.3%) demonstrated eosinophilia, and nine (75%) showed high serum IgE levels. Most patients exhibited normal percentages of Treg cells with reduced CD25, FOXP3, and CTLA-4 expressions, corrected after hematopoietic stem cell transplantation (HSCT). Compared to healthy controls, the TH2-like skewing accompanied by reduced TH17-like responses was observed in cTFH and Treg cells of patients. Overall, nine patients (75%) received immunosuppressants (ISs), and six (50%) underwent HSCT, which was the only treatment revealing sustained control. Sirolimus was used in six patients and showed better control than other ISs. The first cohort from Turkey with long-term follow-up results, comparing typical and atypical cases, provides insights into the outcomes of different therapeutic modalities and T- cell subtype changes in IPEX syndrome.

Complex hydrothermal vent microbial mat communities used to assess primer selection for targeted amplicon surveys from Kama‘ehuakanaloa Seamount

The microbiota of hydrothermal vents has been widely implicated in the dynamics of oceanic biogeochemical cycling. Lithotrophic organisms utilize reduced chemicals in the vent effluent for energy, which fuels carbon fixation, and their metabolic byproducts can then support higher trophic levels and high-biomass ecosystems. However, despite the important role these microorganisms play in our oceans, they are difficult to study. Most are resistant to culturing in a lab setting, so culture-independent methods are necessary to examine community composition. Targeted amplicon surveying has become the standard practice for assessing the structure and diversity of hydrothermal vent microbial communities. Here, the performance of primer pairs targeting the V3V4 and V4V5 variable regions of the SSU rRNA gene was assessed for use on environmental samples from microbial mats surrounding Kama‘ehuakanaloa Seamount, an iron-dominated hydrothermal vent system. Using the amplicon sequence variant (ASV) approach to taxonomic identification, the structure and diversity of microbial communities were elucidated, and both primer pairs generated robust data and comparable alpha diversity profiles. However, several distinct differences in community composition were identified between primer sets, including differential relative abundances of both bacterial and archaeal phyla. The primer choice was determined to be a significant driver of variation among the taxonomic profiles generated. Based on the higher quality of the raw sequences generated and on the breadth of abundant taxa found using the V4V5 primer set, it is determined as the most efficacious primer pair for whole-community surveys of microbial mats at Kama‘ehuakanaloa Seamount.