Retrograde flow is the intracellular flow of the cytoskeleton, and it allows for transportation of the structure from the cell edge towards the cell center. It is important to understand the retrograde flow since it is closely related to biological functions, such as contraction forces, metabolisms and turnovers. In this work, we analyze the retrograde flow in immobile cell types given that most of previous studies focused on mobile ones. We obtain fluorescence images of α-actinin, associated with the actin cytoskeleton, in individual A7r5 cells with a fluorescence microscope. We quantified and visualized the retrograde flow in the cells with particle image velocimetry (PIV) and discussed spatial distribution of the intracellular flows. By calculating the divergence of the retrograde flow, we found that the cells have a source of the field not only in the cell periphery but also at the central region of the cells. Since some of the fluid sources are located at the topological defects, we also hypothesize that the topology of cytoskeletons might be related with the cell functions.