Diversity Of Factors Research Articles

The Genomic Epidemiology of Clinical Burkholderia pseudomallei Isolates in North Queensland, Australia.

Background:Burkholderia pseudomallei, the causative agent of melioidosis, is highly genetically recombinant, resulting in significant genomic diversity. Multiple virulence factors have been associated with specific disease presentations. To date, there are limited data relating to genomic diversity and virulence factors associated with melioidosis cases in North Queensland, Australia. Aim: To describe the genetic diversity of B. pseudomallei and identify virulence factors associated with clinical risk factors and patient outcomes. Methods: Whole genome sequencing of clinical isolates was performed and analysed with clinical data obtained from a retrospective melioidosis cohort study. Results: Fifty-nine distinct sequence types (STs) were identified from the 128 clinical isolates. Six STs comprised 64/128 (50%) isolates. Novel STs accounted for 38/59 (64%) STs, with ST TSV-13 as the most prevalent (n = 7), and were less likely to possess an LPS A genotype or YLF gene cluster (p < 0.001). These isolates were most likely to be found outside the inner city (aOR: 4.0, 95% CI: 1.7-9.0, p = 0.001). ST TSV-13 was associated with increased mortality (aOR: 6.1, 95% CI: 1.2-30.9, p = 0.03). Patients with a history of alcohol excess were less likely to be infected by fhaB3 (aOR 0.2, 95% CI: 0.1-0.7, p = 0.01) or YLF (aOR: 0.4, 95% CI: 0.2-0.9, p = 0.04) positive isolates. Conclusions: There are a significant number of novel sequence types in Townsville, Australia. An emerging novel ST appears to have an association with geographic location and mortality. Ongoing investigation is required to further understand the impact of this ST on the Townsville region.

Dietary Diversity and Nutritional Status Among Rwandan Women Engaged in Agriculture: A Cross‐Sectional Study

ABSTRACTBackgroundDietary diversity is crucial for nutritional adequacy, particularly among women of reproductive age who have increased nutritional needs due to menstruation, pregnancy, and lactation. This is especially important in addressing anemia in Rwanda, which poses significant health risks for both mothers and children. This study assessed the dietary diversity, nutritional status, and related factors among Rwandan women engaged in agriculture.MethodsIn 2022, a cross‐sectional study in Nyamagabe, Karongi, and Nyabihu districts, Rwanda, focused on high malnutrition rates. Agriculture households with children under 5 were sampled using a multistage method. Data, including nutritional status via MUAC and dietary diversity via 24‐h recall, were collected digitally through Kobo Collect.ResultsThe study included 439 respondents, with a mean age of 33 years. Among participants, 359 (81.8%) had low dietary diversity, with Nyamagabe having the highest proportion at 39%. Anemia prevalence was 22.1%, with Karongi having the highest at 10.7%. Factors associated with higher odds of high dietary diversity included the education of the household head (adjusted OR = 6.4, 95% CI: 1.05–39.7), age of women (adjusted OR = 3.03, 95% CI: 1.1–7.8), and wealth status (adjusted OR = 1.66, 95% CI: 0.51–5.4). Conversely, the occupation of women (adjusted OR = 0.13, 95% CI: 0.001–0.19), reading skills (adjusted OR = 0.27, 95% CI: 0.1–0.72), and family size (adjusted OR = 0.63, 95% CI: 0.35–1.1) were associated with lower odds of lower dietary diversity.ConclusionThe findings highlight a significant nutritional challenge among Rwandan women, with low dietary diversity, significant rates of anemia, and food insecurity. The study calls for an urgent need for targeted nutritional interventions to improve dietary diversity and address micronutrient deficiencies among women in agriculture to enhance maternal health and child development, thereby contributing to broader public health goals.

Characteristics of demersal fish community structure during summer hypoxia in the Pearl River Estuary, China.

In recent decades, hypoxic areas have rapidly expanded worldwide in estuaries and coastal zones. The Pearl River Estuary (PRE), one of China's largest estuaries, experiences frequent seasonal hypoxia due to intense human activities and eutrophication. However, the ecological effects of hypoxia in the PRE, particularly on fish communities, remain unclear. To explore these effects, we collected fish community and environmental data in July 2021 during the summer hypoxia development period. The results revealed that bottom-layer dissolved oxygen (DO) in the PRE ranged from 0.08 to 5.71 mg/L, with extensive hypoxic zones (DO ≤ 2 mg/L) observed. Hypoxia has varied effects on fish community composition, distribution, species, and functional diversity in the PRE. A total of 104 fish species were collected in this study, with approximately 30 species (28.6%) exclusively found in hypoxic areas. Species responses to hypoxia varied: species such as Sardinella zunasi, Coilia mystus, and Nuchequula nuchalis were sensitive, while Decapterus maruadsi, Siganus fuscescens, and Lagocephalus spadiceus showed higher tolerance. Within the hypoxia area, dissolved oxygen was the main limiting factor for fish community diversity. Functional diversity (FDiv) decreased with higher dissolved oxygen levels, indicating a potential shift in the functional traits and ecological roles of fish species in response to changing oxygen conditions. Further analysis demonstrated that dissolved oxygen had a significantly stronger effect on fish community structure at hypoxic sites than in the whole PRE. Moreover, other environmental variables also had significant effects on the fish community structure and interacted with dissolved oxygen in the hypoxia area. These findings suggest that maintaining sufficient dissolved oxygen levels is essential for sustaining fish communities and ecosystem health in the PRE. This study provides novel insights into the effects of hypoxia on fish communities in estuarine ecosystems and has significant implications for the ecological health and management of the PRE.

Dialectics and Madurese Language Accent in the Perspective of Psycholinguistic and Sociolinguistic

This research aims to reveal and examine the variety of Madurese dialects found in 4 districts in Madura (Bangkalan, Sampang, Pamekasan, and Sumenep) and their factors, starting from acquisition factors to surrounding environmental factors, as well as their influence on a person's attitude patterns. The research method used is a qualitative research method with a phenomenological approach. The research subjects that will be studied are the Madurese people in general from each district. The data collection instrument used structured interviews involving 3 people from each district and sources from the literature. The data analysis technique used by researchers is qualitative descriptive. Data analysis includes data condensation, data presentation, and drawing conclusions. Based on research results, the diversity of Madurese dialects based on psycholinguistic and sociolinguistic studies is formed through the acquisition factor of Madurese language diversity in the form of vocabulary (dialect) by the human mental lexicon obtained by their social environment. Meanwhile, the diversity of the Madurese language accent is formed from various factors, including cultural, geographical, and social class factors, which influence their attitude patterns.

A PRELIMINARY SURVEY ON DIVERSITY OF AVIFAUNA IN KATWA, PURBA BURDWAN, WEST BENGAL, INDIA

Birds are used as bio-indicator due to their sensitivity to minor environmental changes. A survey was done from July 2022 to February 2023 to study the avifaunal diversity of Katwa. A total of 67 bird species were recorded during the study period. The species were categorized in three namely common resident (CR), not common resident (NCR) and migratory (M) of which 40 are common resident, 24 are not common resident and 3 are migratory. The found common birds species were house crow, common myna, red vented bulbul, house sparrow, spotted dove, asian koel, little ringed plover etc. Among the not common resident avifauna were Long-legged Buzzard, Cotton teal, Pied-cuckoo, Grey Wagtail, yellow Wagtail, White Wagtail etc. and the three migratory birds were Common Pochard, Red-crested Pochard and Northern Pintail. Katwa and adjoining Ajay Baadh has wide variety of trees, wetland, grassland and agricultural land which is the major contributing factor for the rich avifaunal diversity found during the short study period. This is a preliminary effort for documentation of the avifauna, but more studies and suitable conservation strategies are required in the study area.

Associated factors of dietary diversity among schoolchildren in Plateau Central region of Burkina Faso: a cross-sectional study

ContextSchool-age is a dynamic period of growth and development, leading to good health and a productive adult life. Adequate dietary intake provides essential nutrients for growth, health and cognition. However, the practices of adequate nutrition is still not a matter of course for schoolchildren in many countries. The aim of this study was to identify associated factors of dietary diversity among students in public primary school in the Central Plateau Region.MethodMulti-stage sampling was used to select schoolchildren. A semi-structured questionnaire was used to collect information’s of food consumption at home and at school using a 24-h dietary recall method. Binary logistic regression was used to identify variables associated with students' dietary diversity scores (DDS) with statistical significance at p < 0.05, after performing Chi-square test of independence to identify candidates variables at p < 0.25.ResultsThe study involved 560 pupils aged 6 to 14 older, including 52.9% girls and 47.9% boys. Dietary diversity was divided into three classes: low (DDS ≤ 4), medium (DDS = 5) and high (DDS ≥ 6). Thus, 13.4% of students have a low DDS and average in 48.9%, versus 37.7% high. Students in Ganzourgou were twice as likely to have a low DDS (AOR = 2.01, 95% CI:1.00–4.04) compared to those in Oubritenga. Household drinking water source, pupil status and father's occupation were significantly associated with pupils' dietary intake.ConclusionPrimary schoolchildren don’t have good dietary practices in the Plateau Central Region. Promoting dietary diversification in households and balanced meals in school canteens would be necessary to improve the DDS of schoolchildren.Trial registrationClinical Trial Number: 2022_33_/MS/MESRSI/CERS of 02/14/2022.

Enhancing Sustainable Safety Practices in Construction: Insights from Cultural and Socio-Economic Analysis

Exploring cultural diversity and socio-economic factors offers a holistic approach to fostering sustainable safety practices within the construction industry. This study investigates the relationship between cultural and socio-economic factors and their influence on safety practices within the construction industry, focusing specifically on Saudi Arabian construction. Employing a comparative research design, data were gathered through a questionnaire distributed among various stakeholders, including construction workers, safety officers, project managers, supervisors, and other professionals involved in construction projects. Structural equation modeling (SEM) was utilized to analyze the relationships between cultural, socio-economic, and safety practices. The findings showed significant associations of cultural and socio-economic factors with safety practices and attitudes. Specifically, positive workplace norms and values, effective communication styles, higher income levels, and better education and literacy levels were found to positively influence adherence to safety guidelines and the effectiveness of training programs. Conversely, job insecurity exhibited a negative impact on safety-related outcomes and training effectiveness. This study demonstrates the importance of fostering a positive safety culture within organizations, emphasizing leadership commitment, effective communication, and employee engagement. It also highlights the significance of addressing socio-economic factors, such as job security, income levels, and education, to promote a safer work environment and enhance safety practices. Practical implications derived from the research findings offer actionable insights for organizations to develop targeted interventions aimed at improving sustainable safety practices and training effectiveness within the construction industry in Saudi Arabia.

Environmental driving factors of diazotrophic community diversity and activity in Chinese paddy soils

Environmental driving factors of diazotrophic community diversity and activity in Chinese paddy soils

Molecular characterization and virulence factor profiles of Staphylococcus aureus isolated from bovine mastitis in Brazilian herds

Staphylococcus aureus is the main etiological agent of bovine mastitis worldwide and knowledge about its diversity and virulence factors is vital in controlling infections caused by this pathogen. The present study aimed to perform molecular characterization of a population of S. aureus (n=153 strains isolated from 1994 to 2014 in seven Brazilian states) by pulsed-field gel electrophoresis (PFGE) and evaluate their virulence profiles via polymerase chain reaction (PCR). PFGE identified 93 pulsotypes, with the isolates organized into 26 clusters and 20 unique pulsotypes. Predominant pulsotypes were observed, with variations according to the years of isolation and geographic origin of the isolates. Based on the PCR results for the genes encoding agglutination factors (ClfA and ClfB), binding proteins (fibronectin binding protein - FnBPA, elastin binding protein - Ebps, collagen binding protein - Cna), and toxins (Hla, Hlb and Luk-ED), 40 virulence profiles were detected. The frequency of virulence genes ranged from 58 to 98% (clfA:84.3%; clfB and hlb both 81%; hla:71.2%; fnBA:82.3%; Can:94.7%; ebps:58%; and lukED:98%). The existence of prevalent genotypes in some of the Brazilian states and the time period studied suggests that these genotypes are better adapted, with favorable characteristics in host/pathogen relationships. Genes of proven importance for S. aureus pathogenesis in bovine mastitis were widely distributed in genetically divergent populations, suggesting that most of these genes may be interesting candidates in the development of vaccines to control bovine mastitis in Brazil.

Genomic Characterization of Multidrug-Resistant Enterobacteriaceae Clinical Isolates from Southern Thailand Hospitals: Unraveling Antimicrobial Resistance and Virulence Mechanisms.

The emergence and spread of antimicrobial resistance (AMR) among Enterobacteriaceae pose significant threats to global public health. In this study, we conducted a short-term surveillance effort in Southern Thailand hospitals to characterize the genomic diversity, AMR profiles, and virulence factors of Enterobacteriaceae strains. We identified 241 carbapenem-resistant Enterobacteriaceae, of which 12 were selected for whole-genome sequencing (WGS) and genome analysis. The strains included Proteus mirabilis, Serratia nevei, Klebsiella variicola, Klebsiella aerogenes, Klebsiella indica, Klebsiella grimontii, Phytobacter ursingii, Phytobacter palmae, Kosakonia spp., and Citrobacter freundii. The strains exhibited high levels of multidrug resistance, including resistance to carbapenem antibiotics. Whole-genome sequencing revealed a diverse array of antimicrobial resistance genes (ARGs), with strains carrying genes for ß-lactamase, efflux pumps, and resistance to other antibiotic classes. Additionally, stress response, metal tolerance, and virulence-associated genes were identified, highlighting the adaptability and pathogenic potential of these strains. A plasmid analysis identified several plasmid replicons, including IncA/C2, IncFIB(K), and Col440I, as well as several plasmids identical to those found globally, indicating the potential for the horizontal gene transfer of ARGs. Importantly, this study also identified a novel species of Kosakonia spp. PSU27, adding to the understanding of the genetic diversity and resistance mechanisms of Enterobacteriaceae in Southern Thailand. The results reported in this study highlight the critical importance of implementing effective antimicrobial management programs and developing innovative treatment approaches to urgently tackle AMR.

Evaluating initial usability of a hand augmentation device across a large and diverse sample.

The advancement of motor augmentation and the broader domain of human-machine interaction rely on a seamless integration with users' physical and cognitive capabilities. These considerations may markedly fluctuate among individuals on the basis of their age, form, and abilities. There is a need to develop a standard for considering these diversity needs and preferences to guide technological development, and large-scale testing can provide us with evidence for such considerations. Public engagement events provide an important opportunity to build a bidirectional discourse with potential users for the codevelopment of inclusive and accessible technologies. We exhibited the Third Thumb, a hand augmentation device, at a public engagement event and tested participants from the general public, who are often not involved in such early technological development of wearable robotic technology. We focused on wearability (fit and control), ability to successfully operate the device, and ability levels across diversity factors relevant for physical technologies (gender, handedness, and age). Our inclusive design was successful in 99.3% of our diverse sample of 596 individuals tested (age range from 3 to 96 years). Ninety-eight percent of participants were further able to successfully manipulate objects using the extra thumb during the first minute of use, with no significant influences of gender, handedness, or affinity for hobbies involving the hands. Performance was generally poorer among younger children (aged ≤11 years). Although older and younger adults performed the task comparably, we identified age costs with the older adults. Our findings offer tangible demonstration of the initial usability of the Third Thumb for a broad demographic.

Validation of a Questionnaire for Analyzing Teacher Training in Inclusive Education in the field of Teacher Training: Questionnaire (CEFI-R)

Goal: This research aims to investigate the validity, reliability, and factor structure of the teacher training analysis questionnaire in inclusive education in the field of teacher training. Method: The research statistical population included all student-teachers of Farhangian University. The statistical sample of the research included 250 student-teachers who were selected by stratified sampling method. Saeedi's (2008) teachers' attitude towards inclusiveness questionnaire (2008) was used to calculate the convergent validity. Construct validity was investigated through confirmatory factor analysis. The reliability was done using Cronbach's alpha method and retesting and data analysis with S. software. P. S. S. Version 25 and Lisrel version 8.8. Findings: The results of the confirmatory factor analysis confirmed the four factors of diversity, methodology, support, and community participation, as in the original form. In addition, Cronbach's alpha coefficient for the whole scale was 0.81 and was 0.86, 0.83, 0.84, and 0.81 for the diversity, methodology, support, and community participation subscales, respectively. In addition, the retest coefficient with an interval of four weeks was 0.76 for the whole scale and 0.79, 0.78, 0.80, and 0.74 for the diversity, methodology, support, and community participation subscales, respectively. In addition, the correlation coefficients indicated the convergent and diagnostic validity of the questionnaire (P&lt;0.001).

Stand Structure and Functional Traits Determine Productivity of Larix principis-rupprechtii Forests

Forest productivity (FP) depends not only on tree species diversity but also on functional traits, stand structure, and environmental factors; however, causation and relative importance remain controversial. The effects of tree species diversity (species richness), trait community-weighted mean (CWM), forest structure (forest density and maximum DBH), and environmental factors (soil nutrients and elevation) on larch (Larix principis-rupprechtii Mayr) forests’ productivity were investigated, and the relative importance of each factor in determining productivity was quantified. Our results showed that stand structure and functional traits had significant positive effects on the basal area increment (BAI) of larch stands (p &lt; 0.05) and were more important than tree diversity and environmental factors in explaining BAI variations. The contribution ratio of each influencing factor was as follows: stand structure (59%), functional composition (30%), environmental factors (9%), and species diversity (SD) (2%). The biomass ratio hypothesis was more important than the niche complementarity hypothesis in explaining the relationship between species diversity and productivity. The structural equation model showed that environmental factors did not directly affect the BAI in larch forests but indirectly affected the BAI through tree diversity and stand structure. Therefore, in larch forests with low species richness, it is more important to adjust stand structure to maintain overyielding while also considering the influence of environmental factors.

Investigating the differences in driving mechanisms for phytoplankton community composition under various human disturbances in cold regions

In river ecosystems, phytoplankton are essential components, and this study delves into their response to critical environmental indicators. We analyzed the spatiotemporal variations of phytoplankton diversity in cold regions, employing techniques such as Principal Component Analysis (PCA), Spearman correlation coefficient, and Generalized Additive Model (GAM) to unveil the critical environmental factors influencing phytoplankton diversity and habitat suitability under varying degrees of human interference. With 37 monitoring stations across two cold region rivers, phytoplankton and water bodies were monitored in spring, summer, and autumn, resulting in the collection and analysis of 111 phytoplankton samples and 13 environmental indicators at each station. By identifying critical water quality indicators and exploring nonlinear relationships, the study revealed intricate interactions between phytoplankton diversity and environmental factors. Our findings suggest that overall phytoplankton species diversity is lower in rivers with high human interference, with turbidity, NH4+-N, chlorophyll-a, and total phosphorus (TP) deemed critical factors. Conversely, chlorophyll-a, NH4+-N, and electrical conductivity (EC) were identified as important factors in rivers with lower human interference, indicating differences in phytoplankton driving mechanisms between the two rivers. These insights provide valuable references for the sustainable development and effective assessment of cold region river ecosystems in the Anthropocene.

Seed Production in Jalandhar

A key factor in agricultural resilience, crop diversity, and productivity is seed production—particularly in areas like Jalandhar that must meet changing market demands and climate change. This project explores the complexities of seed production in Jalandhar and how it affects agricultural development with the goal of educating researchers, policymakers, and agricultural stakeholders on how to support sustainable seed production systems. Located in the rich plains of Punjab, Jalandhar has become a major centre for the production of seeds, especially for pulses, wheat, and rice. The region's favorable soil and climate, together with cutting-edge farming techniques, are what propel the expansion of the seed business. With the growing use of hybrid and genetically modified seeds, government programs encouraging agricultural output, and growing farmer awareness of the advantages of high-quality seeds, Jalandhar has a huge market potential for seed production. Nevertheless, in order to fully realize the potential of this industry, stakeholders must work together and implement strategic interventions in response to issues including volatile market pricing, restricted financial availability for small-scale farmers, and environmental sustainability concerns. The sector's growth trajectory is further aided by the existence of well-established agricultural research institutions and government policies that are supportive of the industry, providing growing opportunities in both domestic and foreign markets High-quality seeds improve crop yields while also contributing to overall sustainability and profitability in farming operations. Agro-ecologically-suited certified seeds are essential for reducing the danger of pests, illnesses, and unfavorable weather. Encouraging and supporting sustainable seed production systems in Jalandhar benefits all related departments and financial institutions by promoting agricultural development, economic success, and food security. Keywords : Seed production ,Agricultural development ,Market potential ,Sustainable seed systems ,Government initiatives ,Economic prosperity.

Expatriate academics’ adjustment experience at a higher education institution in South Africa

Orientation: Expatriate academics in higher education institutions (HEIs) face several socio-cultural and integration challenges that significantly influence their adjustment. Insufficient support and a non-conducive environment hamper their well-being.Research purpose: This study aims to describe the adjustment experiences of expatriate academics at a HEI in South Africa. Recommendations are made towards creating an enabling environment in which expatriate academics can better facilitate adjustment.Motivation for the study: This study aimed to compete globally, HEIs appoint international academics with scarce skills to enhance their teaching, learning, and research. Expatriate academics are driven by push and pull factors when accepting international opportunities. Entering an unfamiliar environment, implies disruption on a psychosocial, socio-economic and job adjustment level, and if not well-supported results in maladjustment affecting expatriate well-being and job performance.Research design, approach and method: An interpretive, qualitative approach was adopted. Five purposively selected expatriate academics partook in semi-structured interviews and the data were analysed through content analysis.Main findings: A lack of employer support and cultural diversity factors impede participants’ ability to adjust at work and in the community. Having grit and being resourceful in overcoming challenges resulted in better adjustment.Practical/managerial implications: Facilitating collaborative relationships between expatriates and host country nationals, and using supportive services and resources will enable better adjustment.Contribution/value-add: Expatriate academics share their lived experiences of what hindered their adjustment and several recommendations are made to HEIs and foreign academics towards creating an enabling environment promoting adjustment.

Systematic challenges and opportunities in insect monitoring: a Global South perspective.

Insect monitoring is pivotal for assessing biodiversity and informing conservation strategies. This study delves into the complex realm of insect monitoring in the Global South-world developing and least-developed countries as identified by the United Nations Conference on Trade and Development-highlighting challenges and proposing strategic solutions. An analysis of publications from 1990 to 2024 reveals an imbalance in research contributions between the Global North and South, highlighting disparities in entomological research and the scarcity of taxonomic expertise in the Global South. We discuss the socio-economic factors that exacerbate the issues, including funding disparities, challenges in collaboration, infrastructure deficits, information technology obstacles and the impact of local currency devaluation. In addition, we emphasize the crucial role of environmental factors in shaping insect diversity, particularly in tropical regions facing multiple challenges including climate change, urbanization, pollution and various anthropogenic activities. We also stress the need for entomologists to advocate for ecosystem services provided by insects in addressing environmental issues. To enhance monitoring capacity, we propose strategies such as community engagement, outreach programmes and cultural activities to instill biodiversity appreciation. Further, language inclusivity and social media use are emphasized for effective communication. More collaborations with Global North counterparts, particularly in areas of molecular biology and remote sensing, are suggested for technological advancements. In conclusion, advocating for these strategies-global collaborations, a diverse entomological community and the integration of transverse disciplines-aims to address challenges and foster inclusive, sustainable insect monitoring in the Global South, contributing significantly to biodiversity conservation and overall ecosystem health. This article is part of the theme issue 'Towards a toolkit for global insect biodiversity monitoring'.

Uncovering DNA methylation landscapes to decipher evolutionary footprints of phenotypic diversity in chickpea.

Genetic diversity and environmental factors are long believed to be the dominant contributors to phenotypic diversity in crop plants. However, it has been recently established that, besides genetic variation, epigenetic variation, especially variation in DNA methylation, plays a significant role in determining phenotypic diversity in crop plants. Therefore, assessing DNA methylation diversity in crop plants becomes vital, especially in the case of crops like chickpea, which has a narrow genetic base. Thus, in the present study, we employed whole-genome bisulfite sequencing to assess DNA methylation diversity in wild and cultivated (desi and kabuli) chickpea. This revealed extensive DNA methylation diversity in both wild and cultivated chickpea. Interestingly, the methylation diversity was found to be significantly higher than genetic diversity, suggesting its potential role in providing vital phenotypic diversity for the evolution and domestication of the Cicer gene pool. The phylogeny based on DNA methylation variation also indicates a potential complementary role of DNA methylation variation in addition to DNA sequence variation in shaping chickpea evolution. Besides, the study also identified diverse epi-alleles of many previously known genes of agronomic importance. The Cicer MethVarMap database developed in this study enables researchers to readily visualize methylation variation within the genes and genomic regions of their interest (http://223.31.159.7/cicer/public/). Therefore, epigenetic variation like DNA methylation variation can potentially explain the paradox of high phenotypic diversity despite the narrow genetic base in chickpea and can potentially be employed for crop improvement.

Altitudinal Variation in Species Diversity, Distribution, and Regeneration Status of a Secondary Picea Forest in Guandi Mountain, Northern China

Altitude plays a crucial role in shaping the diversity and distribution of species in forest landscapes in mountainous regions. However, existing studies often lack comprehensive analysis of the intricate relationships among environmental factors, disturbances, and species diversity. This study aims to address this gap by thoroughly analyzing major species’ diversity, distribution, and regeneration status, along with their influencing factors, across different altitudinal gradients (1850 to 2350 m). Field surveys were conducted to examine the major species in different vegetation layers of the secondary Picea forest in Guandi Mountain. The key findings from the study include the following: (1) the study area recorded a total of nine species of adult trees, belonging to eight genera in five families, fourteen species of shrubs from eight genera in five families, and fifty-two herb species representing forty-eight genera in twenty-six families. Dominant species varied across layers, with Picea asperata Mast. and Larix principis-rupprechtii Mayr. dominating the arborous layer, Lonicera ferdinandii Franch. and Cotoneaster acutifolius Turcz. in the shrub layer, and Poa pratensis L. and Duchesnea indica (Andrews.) Focke in the herb layer. (2) Altitude significantly influenced species diversity, with the most pronounced effects observed in the shrub and herb layers. (3) Tree species regeneration varied with altitude, with Picea asperata exhibiting the highest regeneration performance, particularly in the high-altitude zone (2250–2350 m). Regeneration parameters were significantly correlated with species diversity indices in both tree and shrub layers. (4) Redundancy analysis revealed that, apart from altitude, cutting (p = 0.015) influenced the species diversity of the tree layer, while annual precipitation (p = 0.006) and temperature (p = 0.01) determined the diversity of the shrub layer. Grazing (p = 0.042) was identified as a determining factor for species diversity in the herb layer. Overall, these findings provide valuable insights into the sustainable management of forest ecosystems in the study area and offer practical guidance for forest management in similar mountainous landscapes worldwide.

Pangenome analysis reveals transposon-driven genome evolution in cotton

BackgroundTransposable elements (TEs) have a profound influence on the trajectory of plant evolution, driving genome expansion and catalyzing phenotypic diversification. The pangenome, a comprehensive genetic pool encompassing all variations within a species, serves as an invaluable tool, unaffected by the confounding factors of intraspecific diversity. This allows for a more nuanced exploration of plant TE evolution.ResultsHere, we constructed a pangenome for diploid A-genome cotton using 344 accessions from representative geographical regions, including 223 from China as the main component. We found 511 Mb of non-reference sequences (NRSs) and revealed the presence of 5479 previously undiscovered protein-coding genes. Our comprehensive approach enabled us to decipher the genetic underpinnings of the distinct geographic distributions of cotton. Notably, we identified 3301 presence-absence variations (PAVs) that are closely tied to gene expression patterns within the pangenome, among which 2342 novel expression quantitative trait loci (eQTLs) were found residing in NRSs. Our investigation also unveiled contrasting patterns of transposon proliferation between diploid and tetraploid cotton, with long terminal repeat (LTR) retrotransposons exhibiting a synchronized surge in polyploids. Furthermore, the invasion of LTR retrotransposons from the A subgenome to the D subgenome triggered a substantial expansion of the latter following polyploidization. In addition, we found that TE insertions were responsible for the loss of 36.2% of species-specific genes, as well as the generation of entirely new species-specific genes.ConclusionsOur pangenome analyses provide new insights into cotton genomics and subgenome dynamics after polyploidization and demonstrate the power of pangenome approaches for elucidating transposon impacts and genome evolution.