Disturbance Of Blood Circulation Research Articles

Curcumol Attenuates Portal Hypertension and Collateral Shunting Via Inhibition of Extrahepatic Angiogenesis in Cirrhotic Rats.

Liver cirrhosis can cause disturbances in blood circulation in the liver, resulting in impaired portal blood flow and ultimately increasing portal venous pressure. Portal hypertension induces portal-systemic collateral formation and fatal complications. Extrahepatic angiogenesis plays a crucial role in the development of portal hypertension. Curcumol is a sesquiterpenoid derived from the rhizome of Curcumae Rhizoma and has been confirmed to alleviate liver fibrosis by inhibiting angiogenesis. Therefore, our study was designed to explore the effects of curcumol on extrahepatic angiogenesis and portal hypertension. To induce cirrhosis, Sprague Dawley rats underwent bile duct ligation (BDL) surgery. Rats received oral administration with curcumol (30mg/kg/d) or vehicle (distilled water) starting on day 15 following surgery, when BDL-induced liver fibrosis had developed. The effect of curcumol was assessed on day 28, which is the typical time of BDL-induced cirrhosis. The results showed that curcumol markedly reduced portal pressure in cirrhotic rats. Curcumol inhibited abnormal splanchnic inflow, mitigated liver injury, improved liver fibrosis, and attenuated portal-systemic collateral shunting in cirrhotic rats. These protective effects were partially attributed to the inhibition on mesenteric angiogenesis by curcumol. Mechanically, curcumol partially reversed the BDL-induced activation of the JAK2/STAT3 signaling pathway in cirrhotic rats. Collectively, curcumol attenuates portal hypertension in liver cirrhosis by suppressing extrahepatic angiogenesis through inhibiting the JAK2/STAT3 signaling pathway.

Особливості перебігу вагітності в жінок із передчасними пологами

Purpose - to determine the peculiarities of the course of pregnancy and childbirth in women who had complications from the group of major obstetric syndromes, clinically manifested by premature birth (PB). Materials and methods. A retrospective clinical-statistical analysis of the course of pregnancy and childbirth of n=69 pregnant women with spontaneous PB at a gestation period of 24-36 weeks and 6 days was carried out. Depending on the period of pregnancy, they were divided into groups: the Group I - 9 pregnant women 24-26 weeks 6 days, the Group II - 28 women 28-31 weeks 6 days, the Group III - pregnant women 32-36 weeks 6 days. The Control group (CG) consisted of 56 practically healthy pregnant women with a healthy reproductive history and an uncomplicated course of this pregnancy. Statistical processing of research results was carried out using standard Microsoft Excel 5.0 and Statistica 6.0 programs. Results. Were received significant differences between the Group I and the CG (p=0.007), in the presence of signs of the threat of termination of pregnancy during the second “wave” of cytotrophoblast invasion, so the chance of giving birth in the early term is 4.2 times higher than at term. In the presence of isthmic-cervical insufficiency (ICN), the reliability of differences between the Groups I and II (p=0.0021), Groups I and III (p=0.012) is observed between the Group I and CG (p=0.00083) and the risk of very early PB is 2.6 times higher than the risk of premature, late premature - 2.2 times. Disturbances in uteroplacental blood circulation have the presence of significant differences between Groups I and II (р=0.0051). There is a direct relationship between the level of blood pressure and blood circulation in the uterine arteries. Therefore, the observed difference is not accidental: in the Group II, preeclampsia was observed significantly more often. Violation of feto-placental hemodynamics when comparing groups demonstrated the existence of statistically significant differences between the Groups I and II (p=0.048), as well as between the Group I and CG (p=0.02). In the presence of clinical signs of fetal growth retardation syndrome (GRS), there is a significant difference between the Groups I and II (р=0.033). Conclusions.The features of the course of pregnancy with premature birth include the threat of termination during the second “wave” of cytotrophoblast invasion; isthmic-cervical insufficiency, which can lead to very early premature birth; vaginal infectious-inflammatory and dysbiotic diseases. Severe forms of placental insufficiency and preeclampsia are more often an indication for termination of pregnancy at 28-33 weeks 6 days. The research was conducted according to principles of Declaration of Helsinki. Protocol of research was proved by local ethical committee, mentioned in institution’s work. A informed sonsennt was collected in order to carry out the research. No conflict of interests was declared by the author.

Age features of the thymocite RNA response in the thymus structural and functional areas to blood circulation disorders in the pool of carotic arteries in rats

Introduction. Ischemic-reperfusion injuries of the brain are accompanied by immunological disorders, which in their turn can extend post-ischemic changes in the brain. The studies of structural and functional changes in the thymus cells caused byischemia-reperfusion of the brain can supplement the existing understanding of the mechanism of the above interaction.
 The aim of the study. To investigate the reaction of thymocyte RNA in the structural and functional areas of the thymus gland to blood circulation disturbances in the carotid artery pool.
 Results. In rats of both age groups, cerebral ischemia-reperfusion reduces the content of RNA in the thymus cells in all the structural and functional areas of the thymus except the deep cortical one in one-month rats and the sub-capsular one in three-month rats. 
 Conclusions. Acute circulatory disorders in the pool of the carotid arteries result in certain changes in RNA content in all the structural and functional areas of the thymus with age-related and structural features available.

Extended paraumbilical perforator flap pedicled with the inferior epigastric artery for coverage of type III circumferential electrical burns of the wrist.

Type III electrical burns on the wrist are characterized by circumferential wounds, three dimensional with sandwich-like necrosis, and progressive blood circulation disturbances. Limb salvage is challenging, and success in meeting this challenge depends on vascular reconstruction and wound coverage. This paper is intended for the following purposes: to investigate the principles of wound debridement, the management of involved blood vessels, and the clinical effects of the extended paraumbilical perforator flap pedicled with the inferior epigastric artery for coverage of type III circumferential electrical burns of the wrist. Thirteen male patients (age, 20-43 years; average, 29 years) were enrolled in the study. After early escharotomy, debridement, and vascular reconstruction, all wounds were repaired with the extended paraumbilical perforator flap pedicled with the inferior epigastric artery. Flap survival was achived in all 13 patients. Subcutaneous liquefaction necrosis and infection beneath the flap occurred in 3 patients. Radial or ulnar artery reconstruction via the great saphenous vein graft was performed in 12 cases. All patients had a mean follow-up of 6-36 months, the flaps demonstrated satisfactory flexibility and texture. Hand function was preserved in 8 patients, and no patients developed abdominal hernia. Thorough debridement, early vascular re-establishment, and wound coverage are essential for the overall limb salvage effort for type III circumferential electrical burns of the wrist. The extended paraumbilical perforator flap may provide a new and appropriate option for the primary repair of extensive soft tissue defects.

VERTEBRAL BASILAR INSUFFICIENCY: PROBLEMS AND PROSPECTS FOR SOLUTIONS

Article contains the review of literature data in vertebrobasilar insufficiency. Characteristic of vertebrobasilar system blood vessels topography. etiology and pathogenesis of brain stem blood circulation disturbances are described. The biochemical and pathophysiological mechanisms of brain tissue ischem^ changes. and main clinical manifestations and modern methods of vertebrobasilar insufficiency diagnostic are discussed.

Effect of Pulmonary Function Training with a Respirator on Functional Recovery and Quality of Life of Patients with Stroke.

A stroke is a sudden onset cerebral blood circulation disorder. It occurs in patients with cerebrovascular disease due to various predisposing factors causing stenosis, occlusion, or rupture of intracerebral arteries, which, in turn, causes acute cerebral blood circulation disturbance and clinically manifests as symptoms and signs of excessive or permanent cerebral dysfunction. It can cause serious harm to patients' physical and mental health. This study aimed to evaluate the effect of Breathe-Link breathing trainers on lung function and the ability to perform activities of daily living in patients with stroke. Sixty patients with stroke were randomly divided into two groups. One group was set as the control group and received routine breathing training. The experimental group received a Breathe-Link trainer based on regular training, with rehabilitation training for 12 weeks as the time node. Respiratory muscle strength, respiratory velocity, respiratory capacity, forced vital capacity (FVC), forced expiratory volume in the first second (FEV1), and rate in the first second (FEV1/FVC) were used to evaluate the respiratory function of patients, and the Barthel index was used to evaluate the ability to perform activities of daily living. Improvements in respiratory function and daily living ability were compared between the two groups. After 12 weeks of training, respiratory muscle strength, respiratory velocity, respiratory volume, FVC, FEV1, FEV1/FVC, and Barthel index of patients in the two groups improved compared with those before training (P < 0.05), and the improvement in the treatment group was better than that in the control group (P < 0.05). Breathe-Link breathing trainers can improve lung function and the ability to perform activities of daily living in patients with stroke, and its effect is acceptable. It can be recommended for clinical use.

Hereditary Multiple Exostoses-A Review of the Molecular Background, Diagnostics, and Potential Therapeutic Strategies.

Hereditary multiple exostoses (HMEs) syndrome, also known as multiple osteochondromas, represents a rare and severe human skeletal disorder. The disease is characterized by multiple benign cartilage-capped bony outgrowths, termed exostoses or osteochondromas, that locate most commonly in the juxta-epiphyseal portions of long bones. Affected individuals usually complain of persistent pain caused by the pressure on neighboring tissues, disturbance of blood circulation, or rarely by spinal cord compression. However, the most severe complication of this condition is malignant transformation into chondrosarcoma, occurring in up to 3.9% of HMEs patients. The disease results mainly from heterozygous loss-of-function alterations in the EXT1 or EXT2 genes, encoding Golgi-associated glycosyltransferases, responsible for heparan sulfate biosynthesis. Some of the patients with HMEs do not carry pathogenic variants in those genes, hence the presence of somatic mutations, deep intronic variants, or another genes/loci is suggested. This review presents the systematic analysis of current cellular and molecular concepts of HMEs along with clinical characteristics, clinical and molecular diagnostic methods, differential diagnosis, and potential treatment options.

Prevalence of Risk Factors for Stroke Patients at UKI General Hospital in 2015

Stroke is a disease caused by disturbance of brain blood circulation influenced by many risk factors such as age, blood pressure, blood sugar, and blood lipid level. This study aimed to determine the prevalence of risk factors that contribute to stroke incidence in stroke patients hospitalized at UKI General Hospital in 2015. The design of this study is retrospective with research methodology descriptive observational. The sample in this study is all cases of a stroke at UKI General Hospital in 2015. The result showed that the highest type of stroke is ischemic stroke (77%) with the largest age group of 40-60 years (51.3%), male sex (60 people). Prevalence risk factors are hypertension stage 2 (52,2%), blood glucose level &lt;200 mg/dl (81,4%) low total cholesterol level (47,8%), LDL level borderline high (23%), low HDL level (44,2%).&#x0D; Keywords: stroke, risk factor, the prevalence

Unified Scale for Assessing the Risk of Thromboembolic Complications – Family Doctor’s Tool for COVID-19 Pandemic

At present one of the most discussed medical problem is a COVID-19 pandemic. The world has already registered 274 408 291 cases of infection, and 5 365 361 lethal consequences. This disease is already known for almost two years, but there are still no clear understanding of its pathophysiology, clinical course and optimal therapeutic tactics. In Ukraine during this period, 3,324,999 patients with COVID-19 were registered, 92,929 people died. It is known that in the course of the COVID-19, severe thromboeembolic complications are often observed. That is why the most important element of therapy in case of acute thromboembolic complications is the timely use of thromboprophylaxis. The objective: is to develop an integrative scale that will summarize the conditions associated with the risk of thrombogenic complications. Materials and methods. The literature was analyzed on the E-library, PubMed, Scopus and Web of Science Core Collection databases to study questionnaires that were previously used in the patients with a therapeutic profile. 143 patients were examined, including 119 healthy people of all ages and 24 patients with metabolic syndrome (MS) over 60 years. General clinical, neurological, instrumental and laboratory examination were conducted. Results. The «Unified scale for assessing the risk of thromboembolic complications» was developed, containing 17 questions and allowing to assess the presence of risk factors and the prognosis of thromboembolic complications. The idea of creating arose on the basis of many years of experience in studying the issues of hemorheological properties of blood, microcirculation and endothelial function in persons of different ages on the basis of the Department of Clinical Physiology and Pathology of Internal Organs of the State Institution «D. F. Chebotarev Institute of Gerontology NAMS Ukraine» under the leadership of acad. NAMS of Ukraine Korkushko O.V. The questions used in the «Thromboembolic Complications Risk Assessment Scale» are intuitive and self-explanatory. It can be used in the form of a survey with a doctor, a nurse, and also independently in the form of a patient survey. The most convenient form of use is independently in the form of a questionnaire, and the most promising is when studying the health status of individuals in large samples, for example, in communities. The link to this questionnaire (https://forms.gle/XZwsGp5GaVuNqZeS9) was presented for the first time at the Ukrainian scientific and practical conference with international participation «Primary health care – the main component of health care in Ukraine (for the World Family Doctor Day)» of the P. L. Shupik (May 19–20, 2021). Conclusion. Blood circulation disturbances are accompanied by considerable number of pathological conditions, where early interventions and understanding of pathological mechanisms will reduce the risk of complications and prevent fatal consequences. The developed questionnaire allows, from the standpoint of both a doctor and a patient, to assess the risk of thromboembolic complications and identify risk factors for the development of thrombosis in routine clinical practice.

Features of the retinal structual and functional state under cardiopylmonary bypass during cardiac surgery

Introduction. Cardiac surgeries using cardiopylmonary bypass (CB) have been successfully performed for more than 60 years, but at present it is necessary to further study the changes in tissue hemodynamics during interventions to minimize possible iatrogenic complications. One of these tissues available for direct observation of the vessels of the microvasculature is the retina. Aim – to study the structural and functional state of the retina in patients who underwent cardiac surgery with the use of CB, and to identify potential factors that negatively affect the retinal blood flow. Materials and methods. The clinical study involved 10 patients (20 eyes). All patients before cardiac surgery using CB and 10–14 days after it underwent visometry, perimetry, non-contact tonometry, biomicroscopy, reverse binocular ophthalmoscopy, fundus photography, optical coherence tomography of the retina of the macular region and optic nerve head with determination of the thickness of the layer of ganglion cell and the layer of nerve fibers. To carry out CB, pharmaco-cold cardioplegia was performed with the Custodiol solution using unified surgical and anesthetic tactics. CB was performed on a device with disposable membrane oxygenators (Strockert, Germany) under normothermic conditions, blood circulation was provided with non-pulsating blood flow with a volumetric flow rate of 2.4–2.8 L/min/m2 at an initial dose of heparin of 300 U/kg. Results. The studies revealed a correlation between changes in the histoarchitectonics of the retina and the level of mean arterial pressure (MAP). So, in 30 % of patients, the appearance of ischemic foci along the vascular arcades was found. In 10 % of patients, a decrease in the thickness of the ganglion layer and the layer of retinal nerve fibers was reported as a result of impaired blood circulation in the vessels supplying the optic nerve. Conclusion. Increase in MAP up to 90 mm Hg and above and fluctuations in its level of more than 20 mm Hg (p&lt;0.05) during cardiac surgery under cardiopulmonary bypass in 67 % of cases is accompanied by transient disturbance of regional blood circulation at the level of vessels of the microvasculature of the retina with the appearance of ischemic foci in the retinal tissue. In this regard, it is necessary to carefully monitor the MAP parameters during the cardiopulmonary bypass to prevent circulatory disorders in various organs and tissues of the body.

Role of acute respiratory diseases in pathogenesis of distal limb infections in cattle

According to current concepts, ruminal and metabolic acidosis occur due to feeding cattle mainly with preserved acidic feeds such as silage and haylage. However, errors in feeding are not the only etiological factor leading to acidosis. In some cases, metabolic acidosis in cattle can develop along with respiratory infection caused by viral and bacterial agents. The main pathological processes resulting from acute respiratory diseases of cattle are bronchitises, tracheites and pneumonias. When the respiratory tract is affected in cattle, hypoxia occurs, causing intoxication and, thus, leading to ruminal acidosis. As a result, vasoactive substances (bacterial endotoxins, histamine, lactate) enter the bloodstream, the vascular endothelium is damaged due to the simultaneous expansion of arterioles and compression of venules, blood fluid is perfused from the vessels into the surrounding tissues, the blood flow in the microcirculatory bed is disrupted. An important role in the disturbance of blood circulation in small blood vessels is played by circulating immune complexes representing the «antigen-antibody» complex. Low molecular weight circulating immune complexes settle in various organs and tissues of the body, lead to inflammation and damage the normal tissue structure. Most frequently, immune complexes affect the endothelium of blood vessels, renal glomeruli and joints. Distal limb vessels are primarily affected in cattle, leading to disturbance of skin trophism of the limbs and hooves, development of laminitis, while the hoof horn is weakly keratinized and cannot resist aggressive mechanical and chemical environmental factors. Damaged hooves are the gateway of infection for the agents of necrobacteriosis (Fusobacterium necrophorum), staphylococcosis (Staphylococcus spp.), streptococcosis (Streptococcus spp.) and other pathogens. In addition, favorable conditions evolve for the development of mixed infection due to reduction in the overall organism resistance, which is observed for both respiratory and distal limb infections.

On the origin and recognition of embryocardia

In extremely severe cases of disturbance of blood circulation, in cases where a catastrophe is imminent, the so-called is observed in patients. embryocardia. It occurs mainly in acute diseases (with typhus and typhoid fever, pneumonia, acute peritonitis, etc.), heralding a near end, but it can also be observed in the last stage of chronic myocardial diseases.

Pharmaceutical care and active treatment in patients with osteochondrosis

According to the International Classification of Diseases, osteochondrosis is included in the group of diseases of the musculoskeletal system, mainly the spine. The mechanism of the disease is expressed in the disturbance of blood circulation, which nourishes the bone, and hence the damage to bone and cartilage tissue. Objective: This paper aims to present the effect of applied pharmacological and active treatment in 10 patients diagnosed with lumbar osteochondrosis. Methods: We conducted a comprehensive treatment of 10 patients diagnosed with lumbar osteochondrosis without any neurological symptoms during the period 2017–2020. This pharmacological and active treatment lasted from 15 to 17 days. The intensity of the pain symptoms, the general mobility of the spine, and the mobility of the lumbar spine were measured to determine the deficit of the patients. The strength of the flexor and extensor muscles of the body was tested. Results: A decrease in pain intensity, increase in the strength of the abdominal and back muscles and improvement of the mobility of the back muscles were found in all patients after the complex treatment. As a result of the therapy, a reduction in the patient’s dysfunctions and the risk of developing disabilities was found. Conclusion: The application of a multidisciplinary approach in patients with osteochondrosis is of great importance for reducing pain intensity and achieving a good functional recovery of motor and stabilizing function of the muscles of the spine.

Thrombectomy from superior mesenteric vein in treatment of small intestine gangrene

The traditional definition of tissue ischemia-a decreased level of oxygen deliverability by bloodstream that results in cell hypoxia. Anatomical and functional hindrance to blood flow is the basis of tissue hypoperfusion. Arterial, venous and functional disturbances in blood circulation lead to acute intestinal ischemia. In 85-90 % of cases acute mesenterial ischemia was caused by arterial reasons: thrombosis or embolism. Venous thrombosis is responsible for 10%-15% of acute mesenterial ischemia cases.

CARDIOVASCULAR SYSTEM OF THE MATURE RATS WITH CONGENITAL HYPOTHYROIDISM AND ARTERIAL HYPERTENSION

The aim: Studying changes in the ultrastructure of blood circulatory capillaries of the myocardium of mature rats with hypothyroidism and arterial hypertension. Materials and methods: Experiments were conducted on (240 days) 10 ISIAH (inherited stress-induced arterial hypertension) line rats with AH (arterial hypertension), 10 Wistar line rats with congenital hypothyroidism and 10 intact animals. Arterial pressure was measured, and the development of hypothyroidism was controlled by the immune enzyme method. The study of the left ventricle myocardium of the rat heart was carried out by electron microscopic and morphometric studies. Results: In in rats with AH the following changes were observed in the blood capillaries of the myocardium: decrease in the number of capillaries; disturbance of blood circulation; the number of organelles of the biosynthetic plan and structures involved in the transendothelial transfer of substances decreased in endothelial cells; lysis and edema of the latter; mucinous perivascular edema, confirmed by the accumulation of fine-fibrillar structures, collagen fibers, cellular detritus. By the same term, in the group with congenital hypothyroidism, dystrophic-destructive changes in the blood capillaries of the myocardium acquired the highest degree, which resulted in a decrease in their number due to destruction. Ultrastructure of the biosynthetic plan organelles and structures of the transendothelial transfer of substances were in decompensated state. Conclusions: The rats (in 240 days) with AH and congenital hypothyroidism express breakdown of compensatory processes in the capillaries of the myocardium. This is manifested by the further dilution of capillaries, the development of hypoxic state in them as well as mucinous edema of interstitium, the decrease of activity of biosynthetic and transport processes.

Progesterone Protects Prefrontal Cortex in Rat Model of Permanent Bilateral Common Carotid Occlusion via Progesterone Receptors and Akt/Erk/eNOS.

Sustained activation of pro-apoptotic signaling due to a sudden and prolonged disturbance of cerebral blood circulation governs the neurodegenerative processes in prefrontal cortex (PFC) of rats whose common carotid arteries are permanently occluded. The adequate neuroprotective therapy should minimize the activation of toxicity pathways and increase the activity of endogenous protective mechanisms. Several neuroprotectants have been proposed, including progesterone (P4). However, the underlying mechanism of its action in PFC following permanent bilateral occlusion of common carotid arteries is not completely investigated. We, thus herein, tested the impact of post-ischemic P4 treatment (1.7mg/kg for seven consecutive days) on previously reported aberrant neuronal morphology and amount of DNA fragmentation, as well as the expression of progesterone receptors along with the key elements of Akt/Erk/eNOS signal transduction pathway (Bax, Bcl-2, cytochromeC, caspase3, PARP, and the level of nitric oxide). The obtained results indicate that potential amelioration of histological changes in PFC might be associated with the absence of activation of Bax/caspase3 signaling cascade and the decline of DNA fragmentation. The study also provides the evidence that P4 treatment in repeated regiment of administration might be effective in neuronal protection against ischemic insult due to re-establishment of the compromised action of Akt/Erk/eNOS-mediated signaling pathway and the upregulation of progesterone receptors.

Effect of axial flap of adjacent artery perforator with vascular pedicle in repairing facial and cervical scar deformity in patients

Objective: To explore the effect of axial flap of adjacent artery perforator with vascular pedicle in repairing scar deformity of face and neck in patients. Methods: From January 2010 to June 2018, 38 patients with cicatricial deformity of face and neck after deep burn were admitted to author's unit, including 22 males and 16 females, aged 5-56 years. The time of admission was 7 months to 19 years after burn injury. The size of wounds ranged from 7.0 cm×4.0 cm to 20.0 cm×10.0 cm after scar tissue was released. Nineteen patients were treated by upper thoracic internal artery perforator flap with size ranged from 7.0 cm×5.0 cm to 18.0 cm×8.5 cm. Among them, 16 cases were preexpanded with expanders at thorax whose rated capacity ranged from 300 to 500 mL and times of water injection of 1.8 to 3.1, and 3 cases were directly used. Twelve patients were treated by transverse carotid artery perforator flap with size ranging from 7.0 cm×4.5 cm to 11.0 cm×8.5 cm, of which 8 patients were preexpanded and 4 cases were directly used. The rated capacity of expander placed at the supraclavicular region ranged from 200 to 350 mL with times of water injection from 1.5 to 2.0. Seven patients were treated by preexpanded superficial temporal frontal branch artery perforator flap with size of 5.5 cm×3.8 cm to 8.0 cm×5.0 cm. The rated capacity of expanders placed at forehead was 150 to 300 mL with times of water injection of 1.5 to 2.0. Donor sites were directly sutured or reversely repaired by pedicled skin tube plasty. After operation, operation times and treatment time were recorded. The survival condition and complications of flaps and follow-up were observed. Results: The patients each received 1 to 4 operation (s) with treatment time of 5 to 11 months. All flaps survived after operation. Among them, three flaps with perforating branches of internal thoracic artery had slight blood circulation disturbance at the distal end and were healed after conservative dressing change, etc. Pigment changes were observed at the distal end of thoracic internal artery perforator flaps in two patients in the later stage and was resected and repaired in the second stage. The patients were followed up for 5 to 18 months. The appearance and function of operation area were good with high satisfaction of patient. Conclusions: The axial flap of adjacent artery perforator with vascular pedicle for repairing scar deformity of face and neck used directly or preexpanded can solve the problem of lack of normal skin around scar deformity without vascular anastomosis during the operation and with better appearance and function after operation. The donor site often can be directly sutured, but many operations often need to be completed for finishing whole treatment.

Сhanges of family diseases in circulation of reproductive system

Modern andrologists diagnose almost permanent pathological changes in seminal vesicles during inflammatory diseases like pelvic organs (prostatitis, cystitis), and the entire urogenital system (pyelonephritis, urethritis, etc.). Thus, seminal vesicles not only lose their function of supporting the life sperm, but also become inflammation, which leads to chronicity of the primary disease and impotence. There is still no clear anatomical idea of the structural changes in seminal vesicles that occur in them during circulatory disorders of the reproductive system, whereas the clinical manifestations of violations in these organs have long been confirmed in practice. Thus, the study of the myogenesis of seminal vesicles with disorders of the circulatory system of the genitourinary system is relevant. Materials and methods: Simulation of circulatory disorders. Access - lower median luporotomy. Separated spermatic cord, after which dressed the catheter tube for all the education of the spermatic cord within 0.5-1 cm. And it was delayed by the ligature. After that, the testicle and spermatic cord were immersed in the abdominal cavity. Advantages: Applying a ligature to the catheter allows a more gentle, dosed to bandage the structures of the spermatic cord. Chris was withdrawn from the experiment after 7 and 14 days. Results and discussion: Macroscopically seminal vesicles without visual pathology. After the withdrawal from the experiment, the rats were taken from parasites and seminal vesicles. We observed an increase in the weight of seminal vesicles 1.2 times on the side of the bandage in comparison with the other. On histological sections, seminal vesicles are represented by multiple transverse sections of the tubules, the walls of which are closely adjacent to each other, between them is a small amount of loose connective tissue and vessels. The tulip of tubules is represented by several rows of rounded epithelial cells with a centrally located round nucleus and light pink cytoplasm. Some epitheliocytes are excised in the lumen of the tubules, their cytoplasm is vacuated, the contours of the nuclei are fuzzy. The stratum is moderately edematous, in the vessels, the phenomena of erythrothesis. In places perivascularly, small clusters of lymphocytes and isolated segmentnucleated leukocytes are observed. Conclusion: With morphometry, an increase in the weight of seminal vesicles was 1.2 times on the side of the dressing compared to the other. At the morphological level, the data obtained indicate the changes that arise in the organs of the reproductive system in the disturbances of blood circulation, in particular, when squeezing the spermatic cord due to the output of the inguinal veins. There was a swelling of the stroma, a stasis of red blood cells, an accumulation of leukocytes, indicating the reactivity of processes arising from circulatory disorders.

STUDI GAMBARAN HASIL PEMERIKSAAN APUSAN DARAH TEPI PADA IBU HAMIL DI LABORATORIUM RSUD H.A.SULTHAN DAENG RADJA KABUPATEN BULUKUMBA

During pregnancy, there will be a marked change in anatomy, physiology and biochemistry since the onset of pregnancy and often lackof nutrient intake. Hematologic disorder is often found in pregnant women because it causes pregnant women more susceptible to disturbances in blood circulation, The purpose of this study to determine the description of blood smear results in pregnant women in the laboratory RSUD H.A.Sulthan Daeng Radja District. This research is descriptive with laboratory observation approach. The sample used in this study were 30 samples of pregnant women who checked themselves in the Laboratory of RSUDH.A.Sulthan Daeng Radka Bulukumba District. The result of this research showed the result of peripheral blood smear on the morphology of erythrocytes using 30 samples of pregnant women showed 14 samples (46,66%) normocytic normochrom and the remaining 16 samples were morphological variation (53,33%), on morphological examination and platelet count with using 30 samples of pregnant women showed each 29 samples had morphology and normal platelet counts with respectively 96.66% percentage and platelet aggregation and decreased platelet count (thrombocytopenia) with each persentase 3.33%. At leukocyte morphology examination using 30 samples of pregnant women showed 29 samples had normal morphology with 96,66% percentage and one sample with hypersegmentation with percentage 3,33%. normal leukocyte count at 9 samples with percentage 30% and leukocyte count increased at 21 samples with percentage 70%.

THE FUNCTIONAL ACTIVITY OF INNATE IMMUNITY CELLS IN BACTERIAL INFECTION ON BACKGROUND OF THERMAL STRESS

Maintenance of thermo homeostasis under the coordinating influence of the hypothalamus is ensured by integrative interaction of various systems organisme, including the immune system. Temperature stress in infectious diseases activates the reaction of heat shock, the biochemical consequence of which is the initiation of the organism’s defense against the pathogen. Cells of innate immunity (neutrophils and macrophages) are the first line of protection against pathogenic agents and play a primary role in the development of bacterial infections. Of particular interest is the study of the duration of the effect of hyperthermia to achieve a balance between the bioenergetic costs of these cells, as well as the study of the course of the pathological process in an organism previously exposed to hige temperature. The functional status of neutrophils and macrophages, including phagocytosis, the activity of enzymes of the oxygen-dependent system: lactate dehydrogenase, cytochrome oxidase, myeloperoxidase, cellular stimulation (intracellular AMPase content) and the content of nitrogen oxide metabolites have been studied in the model of animals exposed to low and high temperatures. It has been established that under hyperthermia conditions, the change in the functional activity of cells by enzyme level is more pronounced than when exposed to animals with low temperature, especially 4 h exposure. In animals pre-exposed to heat stress, manifestations of pseudotuberculosis infection were more severe with an increase in mortality rates by 2.6 times, compared to animals infected by bacteria. These animals had a high stimulation of effector cells of inflammation in the initial periods (at 7 days) their metabolism was enhanced, which was expressed of the activity of enzymes of the oxygen-dependent system, as well as in high nitroxide-producing activity. In target organs (lung, liver, spleen) of experienced animals the severe disturbance of blood circulation in combination with significant destructive changes typical for generalized infection were showed. At dead animals on the background of marked hemorrhagic component pathological process and weak cell inflammatory response observed depletion of the immune system (delimphatization), indicating a decrease in defense reactions and the development of immunodeficiency. Thus, under conditions of heat stress (+30°С), the intensity of the reaction of innate immunity cells in terms of enzyme’s functional activity was higher than when exposed to animals of low temperature (+4°C). Under these temperature conditions, a high level of cell priming was determined, which reduced their killing potential. These data indicate the adequacy of the model used to reproduce induced secondary immunodeficiency in a congenital defense system. Moreover, in the pathogenesis of pseudotuberculosis infection against the background of prolonged action high temperature, the effects of phagocytes oxidative stress in the structural changes of immunocompetent organs were detected.