Distribution Patterns Research Articles

The first Mud Dragons (Kinorhyncha) from the Emperor Seamount Chain (Northwestern Pacific) with notes on their biogeography and distribution patterns in the Pacific Deep-Sea

Seamounts and oceanic currents play crucial roles in shaping the genetic diversity of species by either acting as barriers or pathways for species dispersal. In the meiofaunal samples collected using ROV facilities at the slope of the Koko Guyot (2172 m) in the southernmost part of the Emperor Seamount Chain in the Northwestern Pacific, we found representatives of three kinorhynch genera, Echinoderes cf. lupherorum Sørensen et al. 2018, Campyloderes cf. vanhoeffeni Zelinka, 1913 and Sphenoderes sp. 1., previously known from other locations in the Pacific. Kinorhynchs were studied and illustrated using light (LM) and scanning electron (SEM) microscopy to prove the species identity and to compare morphological variations with representatives of these species collected from other, very distant localities in the Pacific. We illustrate the pan-oceanic distribution of the collected species with a system of abyssal currents in the Pacific. The probable distribution pattern corresponds with the northward pathway of Antarctic Bottom Water out of the Southern Ocean in the Pacific through the deep-sea area off New Zealand. Echinoderes lupherorum appears to be the first representative of the Echinoderidae with the pan-Pacific distribution. Discussions on the biogeography of Campyloderes cf. vanhoeffeni Zelinka, 1913 and Sphenoderes in the Pacific are also provided.

A Study on Revenue Distribution of Chinese Agricultural E-Commerce Supply Chain Based on the Modified Shapley Value Method

With the rapid development of e-commerce in China, “live broadcast + e-commerce” has become an emerging mode of the e-commerce industry. The speedy growth of the digital economy has also provided a favorable external environment for fresh agricultural products to ride on the e-commerce express train. As a result, more and more suppliers of fresh agricultural products choose to sell their products on e-commerce platforms and conduct live streaming. This paper utilizes the e-commerce supply chain field survey data, takes tomato as the specific analyzed variety, and studies the revenue distribution pattern of each subject in the supply chain. The purpose of this paper is to explore the optimization strategy of the revenue distribution of an agricultural e-commerce supply chain, and the results show that the agricultural e-commerce supply chain effectively promotes the matching of supply and demand of medium- and high-end agricultural products, realizes the quality premium of medium- and high-end agricultural products, and significantly improves the overall revenue level of the supply chain. The field survey found that under the supply chain model of “farmers + cooperatives + e-commerce platform”, farmers, cooperatives, and e-commerce platforms gained $2865, $3098, and $1111, respectively, for operating one mu of tomatoes in a year. According to the traditional Shapley value method, from the point of view of the contribution to the cooperative income, part of the income of the farmers and cooperatives should be compensated to the e-commerce platform, and the specific results are as follows: the income of the farmers should be reduced from $2865 to $2729, the income of the cooperatives should be reduced from $3099 to $2955, and the income of the e-commerce platform should be increased from $1111 to $1390. According to the Shapley value method based on the risk coefficient correction, from the perspective of risk compensation, part of the earnings of the e-commerce platform should be compensated to farmers and cooperatives, so as to establish a more equitable and reasonable pattern of earnings distribution, with the specific results: the earnings of farmers should be increased to $3205, the earnings of cooperatives should be increased to $3148, and the earnings of the e-commerce platform should be reduced to $722.

Distributions of Lanostene-Derived Triterpenoids and Glucan Content in the Fruiting Bodies of the Australian Ganoderma Species.

Lanostene-derived triterpenoids and β-glucans are important metabolites in Ganoderma mushrooms associated with benefits to human health. The medicinal value of the Australian Ganoderma species remains unclear, with no data on triterpenoid distribution or glucan content. In the present study, 22 Australian Ganoderma specimens were analyzed for triterpenoid and glucan contents. Thirty-two triterpenoids were identified in the fruiting bodies of 19 of the specimens. Distinct patterns in triterpenoid distribution between laccate and matte fruiting bodies were observed, leading to the classification of four groups of Ganoderma. Most of the glucans in the Ganoderma fruiting bodies were β-glucans (~99%), with a nominal α-glucan content (~1%). The β-glucan content ranged from 19.5 to 43.5% (w/w). A range of antioxidant activities was observed for methanol extracts using the ABTS (1.8 to 8.4 mg GAE.g-1), DPPH (1.7 to 9.4 mg GAE/g-1) and FRAP (24.7 to 111.6 mmol FeSO4.g-1) assays, with four specimens presenting relatively high radical scavenging and reducing activities. For the first time, we demonstrated that Australian Ganoderma mushrooms contain medicinal triterpenoids, including ganoderic acid A, and we established a link between its distribution and the fruiting body morphology. However, further research is required to isolate diploid clones and determine factors that impact triterpenoid and glucan synthesis in these strains.

Does time matter? Intraspecific diversity of ribosomal RNA genes in lineages of the allopolyploid model grass Brachypodium hybridum with different evolutionary ages

BackgroundPolyploidisation often results in genome rearrangements that may involve changes in both the single-copy sequences and the repetitive genome fraction. In this study, we performed a comprehensive comparative analysis of repetitive DNA, with a particular focus on ribosomal DNA (rDNA), in Brachypodium hybridum (2n = 4x = 30, subgenome composition DDSS), an allotetraploid resulting from a natural cross between two diploid species that resemble the modern B. distachyon (2n = 10; DD) and B. stacei (2n = 20; SS). Taking advantage of the recurrent origin of B. hybridum, we investigated two genotypes, Bhyb26 and ABR113, differing markedly in their evolutionary age (1.4 and 0.14 Mya, respectively) and which resulted from opposite cross directions. To identify the origin of rDNA loci we employed cytogenetic and molecular methods (FISH, gCAPS and Southern hybridisation), phylogenetic and genomic approaches.ResultsUnlike the general maintenance of doubled gene dosage in B. hybridum, the rRNA genes showed a remarkable tendency towards diploidisation at both locus and unit levels. While the partial elimination of 35S rDNA units occurred in the younger ABR113 lineage, unidirectional elimination of the entire locus was observed in the older Bhyb26 lineage. Additionally, a novel 5S rDNA family was amplified in Bhyb26 replacing the parental units. The 35S and 5S rDNA units were preferentially eliminated from the S- and D-subgenome, respectively. Thus, in the more ancient B. hybridum lineage, Bhyb26, 5S and 35S rRNA genes are likely expressed from different subgenomes, highlighting the complexity of polyploid regulatory networks.ConclusionComparative analyses between two B. hybridum lineages of distinct evolutionary ages revealed that although the recent lineage ABR113 exhibited an additive pattern of rDNA loci distribution, the ancient lineage Bhyb26 demonstrated a pronounced tendency toward diploidisation manifested by the reduction in the number of both 35S and 5S loci. In conclusion, the age of the allopolyploid appears to be a decisive factor in rDNA turnover in B. hybridum.

Distribution and characterization of endophytic and rhizosphere bacteriome of below-ground tissues in Chinese fir plantation.

None declared.Conflict of interestPlantation of Chinese fir, a popular woody tree species, faces sustainable issues such as nutrient deficiency and increasing disease threat. Rhizosphere and endophytic bacteria play important roles in plant' nutrient absorption and stress alleviation. Our understanding on the microbiome structure and functions are proceeding rapidly in model plants and some crop species. Yet, the spatial distribution and functional patterns of the bacteriome for the woody trees remain largely unexplored. In this study, we collected rhizosphere soil, non-rhizosphere soil, fine root, thick root and primary root samples of Chinese fir, and investigated the structure and distribution of bacteriome, as well as the beneficial effects of endophytic bacterial isolates. We discovered that Burkholderia and Paraburkholderia genera were overwhelmingly enriched in rhizosphere soil and the abundance of Pseudomonas genus was significantly enhanced in fine root. By isolating and testing the nutrient absorption and pathogen antagonism functions of representative endophytic bacteria species in Pseudomonas and Burkholderia, we noticed that P-solubilising functional isolates was enriched in fine root, while pathogen antagonism isolates was enriched in thick root. As a conclusion, our study revealed that the endophytic and rhizosphere environments of Chinese fir hold distinct structure and abundance of bacteriome, with potential specific functional enrichment of some bacterial clades. These findings assist us to further study the potential regulation mechanism of endophytic functional bacteria by the host tree, which will contribute to beneficial microbe application in forestry plantation and sustainable development.

A new Boulenophrys species (Anura, Megophryidae) from the coastal hills of eastern Fujian Province, China.

A new species of the genus Boulenophrys is described from the coastal hills of eastern Fujian Province, China. The new taxon can be distinguished from all recognized congeners by a combination of discrete morphological character state differences and genetic divergences in the combined mitochondrial 16S + CO1 genes. We also provide a map showing the distribution pattern of Boulenophrys species in Fujian and a provincial-specific key, which will aid their conservation by helping the local authorities accurately identify species during field identifications and data collection efforts.

In Vitro Analysis of Aerodynamic Properties and Co-Deposition of a Fixed-Dose Combination of Fluticasone Furoate, Umeclidinium Bromide, and Vilanterol Trifenatate.

Effective airway delivery of a fixed-dose combination of triple-aerosolized inhaled corticosteroid (ICS)/long-acting beta agonist (LABA)/long-acting muscarinic antagonist (LAMA) is likely to positively affect therapeutic responses predicted in patients with asthma and chronic obstructive pulmonary disease. This study aimed to conduct in vitro fluticasone furoate, vilanterol trifenatate, and umeclidinium bromide depositions in a Next Generation Impactor. The aerodynamic properties of these inhaled medications influence the spatial distribution and drug abundance, particularly in the smaller airways, to reverse or alleviate disease pathology. The Next Generation Impactor was used to demonstrate the aerodynamic particle size distributions of fluticasone furoate, vilanterol trifenatate, and umeclidinium bromide delivered from a dry powder inhaler at different flow rates across all stages of the impactors. This in vitro study analyzed the distribution pattern of individual drug components to simulate mono-component deposition and co-deposition in the official model in the United States Pharmacopeia. An Andersen cascade impactor together with scanning electron microscope-energy-dispersive X-ray was employed to observe the drug deposition on each stage of the impactor. We found that the distribution pattern of each component at the same cascade level was comparable, and the aerosol particles of the three drugs reached the in vitro representation of the lower airway compartment. The specified flow rates generated the desired fine particle fraction, fine particle dose, and mass median aerodynamic diameter. Our results also demonstrated visualized deposition patterns of the delivered drugs from different stages of the cascade impactor that may predict deposition as it occurs in vivo. Spatial distribution and abundance of ICS/LABA/LAMA in the same cascade levels were closely comparable, and the aerosol particles were able to reach the small aerosol-sized cascades at the lower levels to some extent.

Combined study of Au-bearing arsenopyrite of orogenic gold deposits (NE Asia): High resolution 3D X-ray computed tomography, LA-ICP-MS, and EMPA data

The distribution of gold in small acicular arsenopyrite of a pyrite-arsenopyrite association from Suzdal (Eastern Kazakhstan), Olympiada (Yenisei Ridge, Russia) and large pseudorhombic arsenopyrite crystals from Bazovskoe (Yakutia, Russia) orogenic-type deposits were investigated. On orogenic gold deposits in NE Asia, occurring mainly in black shales, two productive stages of ore deposition are distinguished, which correspond to two morphological varieties of arsenopyrite. At the early stage, fine-grained acicular-prismatic arsenopyrite with invisible gold was deposited; at the late stage, tabular arsenopyrite in association with free visible gold was formed. The samples of gold-bearing arsenopyrite were analyzed using Scanning Electron Microscopy, Electron Microprobe Analyses, Atomic Absorption and Laser Ablation Inductively Coupled Plasma Mass Spectrometry in combination with High Resolution 3D X-ray Computed Tomography (HRXCT). HRXCT does not destroy the studied mineral during the investigation. That technique permits to do an estimation of the amount of gold inclusions in minerals or host rocks and draw reasonable conclusions about the gold content of the ores, to study in detail the distribution patterns of metal inclusions (associated with certain minerals, cracks, crystal growth faces, etc.) and to determine the form of the gold. It can be used to understanding of the genesis of productive mineral associations, and to developing optimal technological schemes for gold extraction.

Distribution Patterns of Tumors and Tumor-Like Lesions of the Forefoot and Midfoot A 12.5-Year Study at a University Hospital.

Masses in the forefoot and midfoot are common reasons for medical presentation and can be caused by various pathological conditions. The challenge in clinical practice is to distinguish the multitude of trivialities from the few malignant entities and to arrive at a reliable clinical diagnosis in a reasonable amount of time with a moderate use of diagnostic tools. In a retrospective analysis, tumors, tumor-like lesions, and pseudotumors distal to the Chopart joint presented to our multidisciplinary university tumor board between January 2010 und June 2023 were analyzed concerning entity, location, age, and sex. Of the 167 cases included, 18 were osseous and 149 were soft tissue lesions. Overall, the metatarsal region was most frequently affected, accounting for 42.5% of all cases. Osseous lesions showed a preference for the phalanges and soft-tissue lesions occurring more frequently in the metatarsal region. In total, 88.0% of all cases were benign. All 20 malignant cases derived from soft tissue, occurred in all sections of the forefoot and midfoot and comprised 13 entities. Most lesions affected middle-aged patients, but cases occurred in almost every age group. In the examined patient population of a German university hospital, most cases were benign soft tissue lesions with a substantial share of pseudotumors and tumor-like lesions. However, the malignancy rate of 12.0% highlights the importance of differential diagnostic considerations. In cases of uncertain results, it is crucial to refer individuals with unclear masses to a specialized center for musculoskeletal tumor care early on in their treatment process. III.

Levantine wines in Italy between the Early Empire and Late Antiquity

The aim of this paper is to investigate the patterns of importations of Levantine wines to Italy between the Early Empire and Late Antiquity. It concentrates on quantities of Levantine amphoras discovered in terrestrial contexts in Rome, Ostia, Campania and the North Adriatic part of Italy. This data is compared with published discoveries from other parts of Italy and the Roman Mediterranean, including finds from shipwrecks. Regression analyses is used in order to compare distribution patterns of Levantine amphoras in the Mediterranean (Rome, North Adriatic Italy, Spain, Gaul, Ephesus and Cyprus) during the Principate and Late Antiquity. The results of this study show that Levantine wines, and in particular Gazan varieties, became much more popular in the Later Roman and Early Byzantine periods than they had been during the first centuries of the Empire. Several explanations of this phenomenon are considered, including climate change and technological development in the Levant, as well as a structural transformation of the Roman economy.

Geochemistry and Mineralogy of Upper Paleozoic Coal in the Renjiazhuang Mining District, Northwest Ordos Basin, China: Evidence for Sediment Sources, Depositional Environment, and Elemental Occurrence

This study aims to investigate the depositional environment, sediment sources, and elemental occurrence of Upper Paleozoic coal in the Renjiazhuang Mining District, Western Ordos Basin. Furthermore, SEM-EDX, optical microscope (OM), ICP-AES, ICP-MS, and AAS were used. Compared with hard coal of the world, M3 coals were enriched in Ga, Li, Zr, Be, Ta, Hf, Nb, Pb, and Th, M5 coals were enriched in Li (CC = 10.21), Ta (CC = 6.96), Nb (CC = 6.95), Be, Sc, Ga, Hf, Th, Pb, Zr, In, and REY, while M9 coals were enriched in Li (CC = 14.79), Ta (CC = 5.41), Ga, W, Hf, Nb, Zr, Pb, and Th. In addition, minerals were mainly composed of kaolinite, dolomite, pyrite, feldspar, calcite, and quartz, locally visible minor amounts of monazite, zircon, clausthalite, chalcopyrite, iron dolomite, albite, fluorite, siderite, galena, barite, boehmite, and rutile. In addition, maceral compositions of M3 coals and M9 coals were dominated by vitrinite (up to 78.50%), while M5 coals were the main inertite (up to 76.26%), and minor amounts of liptinite. REY distribution patterns of all samples exhibited light REY enrichment and negative Eu anomalies. The geochemistry of samples (TiO2 and Al2O3, Nb/Y and Zr × 0.0001/TiO2 ratios, and REY enrichment types) indicates that the sediment sources of samples originated from felsic igneous rocks. Indicator parameters (TPI, GI, VI, GWI, V/I, Sr/Ba, Th/U, and CeN/CeN*) suggest that these coals were formed in different paleopeat swamp environments: M3 coal was formed in a lower delta plain and terrestrial (lacustrine) facies with weak oxidation and reduction, and M5 coal was formed in a terrestrial and dry forest swamp environment with weak oxidation–oxidation, while M9 coal was formed in a seawater environment of humid forest swamps and the transition from the lower delta plain to continental sedimentation with weak oxidation and reduction. Statistical methods were used to study the elemental occurrence. Moreover, Li, Ta, Hf, Nb, Zr, Pb, and Th elements were associated with aluminosilicates, and Ga occurred as silicate.

Prediction of the potential distribution of a raspberry (Rubus idaeus) in China based on MaxEnt model

Rubus idaeus is a pivotal cultivated species of raspberry known for its attractive color, distinct flavor, and numerous health benefits. It can be used in pharmaceutical, cosmetics, agriculture and food industries not only as fresh but also as a processed product. Nowadays due to climatic changes, genetic diversity of cultivars has decreased dramatically. However, until now, the status of wild R. idaeus resources in China have not been exploited. In this study, we investigated the resources of wild R. idaeus in China to secure its future potential and sustainability. The MaxEnt model was used to predict R. idaeus suitable habitats and spatial distribution patterns for current and future climate scenarios, based on wild domestic geographic distribution data, current and future climate variables, and topographic variables. The results showed that, mean temperature of the coldest quarter (bio11), precipitation of the coldest quarter (bio19), precipitation of the warmest quarter (bio18), and temperature seasonality (bio4) were crucial factors affecting the distribution of R. idaeus. Presently, the suitable habitats were mainly distributed in the north of China including Xinjiang, Inner Mongolia, Gansu, Ningxia, Shaanxi, Shanxi, Hebei, Beijing, Liaoning, Jilin, Heilongjiang. According to our results, in 2050s, the total suitable habitat area of R. idaeus will increase under SSP1-2.6 and then will be decreased with climate change, while in the 2090s, the total suitable habitat area will continue to decrease. From the present to the 2090s, the centroid distribution of R. idaeus in China will shift towards the east and the species will always be present in Inner Mongolia. Our results provide wild resource information and theoretical reference for the protection and rational utilization of R. idaeus.

Diversity and abundance of large old trees in Hainan Island: Spatial analysis and environmental correlations

AbstractLarge old trees (LOTs) are invaluable natural treasures and cultural legacies, holding ecological, historical, social, and economic significance. However, climate change and human impacts threaten their existence. Understanding their species composition, age structure, biomass, and distribution patterns in relation to environmental factors is essential for effective conservation. In Hainan Island, South China, we analyzed 1081 LOTs from 74 species across 35 families, using a combination of field surveys and government data. The dominant families were Moraceae, Sapindaceae, and Fagaceae, with key species including Ficus altissima, Litchi chinensis, and Tamarindus indica. The age distribution followed a reverse J‐shape, indicating a prevalence of trees aged 300–399 years. In contrast, diameter at breast height (DBH) and height distributions were unimodal, favoring medium to large‐sized trees. Spatial analysis revealed uneven distribution patterns: the western zone exhibited the highest species diversity, the central zone moderate diversity, and the eastern zone low diversity, reflecting uneven urban and agricultural impacts. Most LOTs were concentrated at elevations of 0–50 m. Factors such as GDP, urbanization, and forest cover positively correlated with LOT abundance and diversity, with forest cover and average annual temperature particularly favoring these trees. The findings emphasize the intricate relationship between environmental factors and LOT distribution. They advocate for tailored conservation strategies that address the complex interplay of ecological, socioeconomic, and cultural influences on their preservation.Abstract in Chinese is available with online material.

An internal loop region is responsible for inherent target specificity of bacterial Cold-shock proteins.

Cold shock proteins (Csps), of around 70 amino acids, share a protein fold for the cold shock domain (CSD) that contains RNA binding motifs, RNP1 and RNP2, and constitute one family of bacterial RNA-binding proteins. Despite similar amino acid composition, Csps have been shown to individually possess inherent specific functions. Here we identify the molecular differences in Csps that allow selective recognition of RNA targets. Using chimeras and mutants of Escherichia coli CspD and CspA, we demonstrate that Lys43-Ala44 in an internal loop of CspD and the N-terminal portion with Lys4 of CspA are important for determining their target specificities. Pull-down assays suggest these distinct specificities reflect differences in the ability to act on the target RNAs rather than differences in binding to the RNA targets. A phylogenetic tree constructed from 1,573 Csps reveals that the Csps containing Lys-Ala in the loop form a monophyletic clade, and the members in this clade are shown to have target specificities similar to E. coli CspD. The phylogenetic tree also finds a small cluster of Csps containing Lys-Glu in the loop, and these exhibit different specificity than E. coli CspD. Examination of this difference suggests a role of the loop of CspD type proteins in recognition of specific targets. Additionally, each identified type of Csp shows a different distribution pattern among bacteria. Our findings provide a basis for subclassification of Csps based on target RNA specificity, which will be useful for understanding of the functional specialization of Csps.

Taxonomic revision of the extant species of the Japanese Stephanidae (Hymenoptera), with discussion on distribution pattern and conservation importance

Taxonomic revision of the extant species of the Japanese Stephanidae (Hymenoptera), with discussion on distribution pattern and conservation importance

Multi-scale modeling and mechanical performance analysis of finger seals with plain woven C/C composite

The application of carbon fibers reinforced carbon matrix (C/C) composite can solve the local wear of metallic finger seals effectively. However, the performance of C/C composite finger seal is complex and variable, which further decreases the sealing performance and life. Therefore, a method of multi-scale modeling and mechanical performance analysis for plain woven C/C composite finger seals was conducted. The circumferential finger beams of C/C composite were modeled by multi-scale structural analysis and weaving simulation. The radial static and dynamic stiffness characteristics of finger beams were investigated. The results showed that the radial static stiffness of the finger beam with three layers was about 3 times that with single layer. The radial stiffness of circumferential finger beams presented a periodic distribution pattern with a period of 90°. The radial dynamic stiffness of C/C composite finger beams increased with the excitation displacement amplitude and rotor speed. But the magnitude and fluctuation degree of dynamic stiffness were greater than those of static stiffness. A large difference in radial stiffness will lead to local wear and hysteretic leakage. This study lays a foundation for the analysis and optimization of the hysteresis and wear characteristics of C/C composite finger seals.

A Novel Method for Assessing Pirate Attack Risks and Spatial Distribution

Pirate attacks pose one of the most severe challenges to the safety of maritime navigation. Effectively quantifying the risk of pirate attacks and understanding their spatial distribution through historical records is crucial for planning safe shipping routes. Given the diverse data types and multiple factors involved in assessing pirate attacks recorded in the Global Integrated Shipping Information System (GISIS) database, we propose a spatiotemporal influence factor analysis model based on the K-means clustering algorithm. Features are encoded using an Autoencoder, and the evaluation is conducted using the Entropy Weight Method- Technique for Order Preference by Similarity to Ideal Solution (EWM-TOPSIS). The model then simulates and predicts the geographical distribution of pirate risks. The results indicate that the model effectively captures the geographical distribution patterns of pirate attack incidents and successfully predicts the risk distribution across different sea areas. This approach aids in ship route planning and reduces the risk of pirate attacks.

Effect of the water erosion on the non-equilibrium condensation in steam turbine cascade

In the final stage of steam turbines, droplets formed by non-equilibrium condensation continuously impact and erode the blades, leading to changes in surface roughness and leading edge erosion. This study investigates the effects of roughness changes and leading edge erosion, induced by water erosion, on non-equilibrium condensation in steam turbine cascades using numerical simulation methods. In a Moses-Stein nozzle, the mechanism of roughness influencing non-equilibrium condensation is analyzed. Subsequently, entropy generation theory is applied to assess the loss distribution caused by roughness variations in a Dykas cascade. Furthermore, flow field analysis and entropy generation theory are utilized to examine the loss distribution pattern resulting from leading edge erosion. Results show that increased roughness delays non-equilibrium condensation, reducing average outlet humidity (from 0.05351 to 0.04361 as roughness rises from 0 to 500 µm). The effect of roughness on thermodynamic entropy generation exhibits a non-linear relationship, balancing steam flow losses with mitigated phase-change-related losses at a roughness of 896 µm. Significant blade erosion (>3 mm) alters leading edge geometry, causing local flow separation and a substantial increase in airfoil losses.

Metagenomic analysis of sedimentary archives reveals ‘historical’ antibiotic resistance genes diversity increased over recent decades in the environment

Abstract Antibiotic Resistance Genes (ARGs) are widespread in freshwater environments and represent a concealed threat to public health and aquatic eco-system safety. To date, only a limited number of studies have investigated the historical distribution of ARGs and their hosts through the analysis of freshwater sedimentary archives. This research gap constrains our comprehensive of the mechanisms underlying natural bacterial resistance formation during pre-antibiotic era (prior to the 1940s) and the development of human-induced bacterial resistance in post-antibiotic era (since the 1940s). In this study, we examined the vertical distribution patterns of ARGs and their associated hosts within a sedimentary core from a eutrophic lake, employing shotgun sequencing methodology. The findings revealed a marked increase in ARG diversity during post-antibiotic era, and the predominant ARG types identified included those conferring resistance to multidrug, bacitracin, macrolide–lincosamide–streptogramin, beta-lactam, tetracycline, fluoroquinolone, glycopeptide and aminoglycoside, collectively accounting for 78.3%–85.6% of total ARG abundance. A total of 127 ARG subtypes were identified in samples, and 48 ARG subtypes shared across vertical sediment resistome profile with two of them, bacA and bcrA, occurring only in post-antibiotic era. Further, 137 metagenome-assembled genomes (83 species belonging to 12 phyla) were identified as ARG hosts, mainly belonging to the phyla Proteobacteria, Nitrospirota, Chloroflexota, Bacteroidota, Actinobacteriota, Cyanobacteria, and Firmicutes. Significant correlation was found between the diversity of ARG and the concentrations of organic matter and heavy metals, suggesting a common source of contamination. Aside the fact that human-induced eutrophication is a forcing factor acting in parallel to increase ARGs releases in water systems, both being indicators of increased urbanization in the catchment, eutrophication may significantly increase bacterial activity, thereby facilitating the spread of antibiotic-resistant bacteria in environment. This study reveals the marked increased in ARG diversity with the onset of antibiotic use by human societies with potential impact of aquatic ecosystem.

Coupling and Coordination Analysis of High-Quality Agricultural Development and Rural Revitalization: Spatio-Temporal Evolution, Spatial Disparities, and Convergence

The coupling and coordination of high-quality agricultural development (HQAD) and rural revitalization is an inevitable choice to accelerate the realization of Chinese-style agricultural and rural modernization. Based on system theory, this study reconstructs the indicator systems of both and conducts measurements by applying the improved AHP–entropy weight method. This study has extended the analytical methods of kernel density estimation, Dagum Gini coefficient, σ convergence, and spatial β convergence to further investigate the spatio-temporal evolution, regional disparities, and convergence effect of the coupling coordination degree (CCD) of HQAD and rural revitalization in China from 2010 to 2020. The results show that the CCD has a tendency to increase year by year, presenting the characteristics of ‘high coupling degree–low comprehensive development level–low coupling coordination degree’, and also has the spatial distribution pattern of ‘high in the east and low in the west’. In addition, most of the provinces have a tendency to jump to a higher stage of coupling coordination; the overall trend of the kernel density curves is favorable; the results of Dagum’s Gini coefficient show that inter-regional disparities contribute the most to regional spatial disparities; and there is a significant tendency towards σ convergence and spatial β convergence of the CCD in China and the four regions. This study stimulates a broader discussion of rural revitalization, with potential implications for decision making in practice.