Distinct Samples Research Articles

Acting as we feel: Which emotional responses to the climate crisis motivate climate action

The current study assessed emotional responses and emotion regulation strategies to the climate crisis, and their relationship to pro-environmental behaviour cross-sectionally using self-report online surveys. 1307 participants were recruited through convenience sampling from six European countries, alongside a distinct sample of 1040 participants representative of age, sex, and ethnicity in the United States. Our findings replicated the well-known association that stronger negative emotions to the climate crisis are associated with more pro-environmental behaviour. The relationship between climate emotions and pro-environmental behaviour was moderated by resignation in the US sample, by cognitive reappraisal and other-blame in the European sample and mediated by rumination in both samples. Furthermore, latent profiles of emotional responses were identified. In both samples, there was one distinct class demonstrating strong climate emotions, and a group with very low or no climate emotions (alongside with two/three groups with moderate emotional intensity in the European and the US samples, respectively). Findings also revealed that members of the emotional group were more likely to take climate action and tend to engage more in emotion regulation than the unemotional group. Our results highlight the crucial role of emotions and emotion regulation strategies in mitigating the climate crisis by taking pro-environmental action.

Multiproxy studies on the spatially distinct surface samples to reconstruct palaeoecology and palaeoclimate from the Core Monsoon Zone of India

Palaeoclimatic reconstructions necessitate an understanding of the various biotic and abiotic responses to develop a modern analogue. The interpretation and calibration of the modern data allow us to check the lead-lag effect in different proxy parameters, which could be applied for robust palaeoclimatic reconstructions. We, in the present study, analysed pollen and diatom (biotic proxies), as well as grain size, magnetic susceptibility and geochemistry (abiotic components) of the modern soil samples, collected from the states of Madhya Pradesh and Chhattisgarh, the Core of the Monsoon Zone (CMZ) in central India. The weathered materials of the Palaeocene Cretaceous extrusive rocks and sedimentary rocks of the Late Triassic to the Upper Carboniferous are underlying the soil cover in these areas, respectively. The study revealed that the overall pollen and diatom preservation is comparatively good in the areas where the Palaeocene Cretaceous extrusive rocks are found except for the areas of human settlements, whereas the preservation of pollen and diatom was comparatively poor in areas where sedimentary rocks of the Late Triassic to Upper Carboniferous are found. The most plausible reason for this difference is the availability of nutrients which are supplied more abundantly by the easily weatherable Deccan basalt rocks compared to their sedimentary counterparts. The present study will serve as baseline information about biotic-abiotic interactions operating in the central Indian Core Monsoon Zone (CMZ). Since the intensity and duration of the rainfall in the CMZ are largely governed by the annual Indian summer monsoon rainfall (AISMR), therefore, the present study could help trace the weak or intense monsoon periods (break and active spells, respectively) of the past hundreds to thousands of years by studying the sediment profiles/cores.

Microstructure, Hardness, Wear Resistance, and Corrosion Resistance of As-Cast and Laser-Deposited FeCoNiCrAl0.8Cu0.5Si0.5 High Entropy Alloy

FeCoNiCrAl0.8Cu0.5Si0.5 high-entropy alloys were fabricated using vacuum induction melting and laser deposition processes, followed by a comparison of the structural and mechanical properties of two distinct sample types. The as-cast FeCoNiCrAl0.8Cu0.5Si0.5 alloy is comprised of BCC1, BCC2, and Cr3Si phases, while the laser-deposited alloy primarily features BCC1 and BCC2 phases. Microstructural analysis revealed that the as-cast alloy exhibits a dendritic morphology with secondary dendritic arms and densely packed grains, and the laser-deposited alloy displays a dendritic structure without the formation of granular interdendritic regions. For mechanical properties, the as-cast FeCoNiCrAl0.8Cu0.5Si0.5 alloy demonstrated higher hardness than the as-deposited alloy, with values of 586 HV0.2 and 557 HV0.2, respectively. The wear rate for the as-cast alloy was observed at 3.5 × 10−7 mm3/Nm, with abrasive wear being the primary wear mechanism. Conversely, the as-deposited alloy had a wear rate of 9.0 × 10−7 mm3/Nm, characterized by adhesive wear. The cast alloy exhibited an icorr of 4.062 μA·cm−2, with pitting as the form of corrosion. The laser-deposited alloy showed an icorr of 3.621 μA·cm−2, with both pitting and intergranular corrosion observed. The laser-deposited alloy demonstrated improved corrosion resistance. The investigation of their microstructure and mechanical properties demonstrates the application potential of FeCoNiCrAl0.8Cu0.5Si0.5 alloys in scenarios requiring high hardness and enhanced wear resistance.

Magmatic conditions aiding synconvergent extension above the Peruvian flat slab

Abstract The Cordillera Blanca and Cordillera Huayhuash contain some of the highest topography in the Andes and provide insight into tectonomagmatic processes associated with the onset of flat-slab subduction. These adjacent ranges shared a similar history of deformation and exhumation prior to the late Miocene, when synconvergent extension began in the Cordillera Blanca. Magmatism in the Cordillera Huayhuash has been inferred as coeval with magmatism in the Cordillera Blanca. Yet, extension, which has been correlated with magmatic heat flow, is limited to the Cordillera Blanca. New zircon U-Pb dates and trace and rare earth element concentrations from the Cordillera Blanca batholith and the Huayllapa pluton in the Cordillera Huayhuash and reassessment of existing zircon data help to characterize regional magmatic processes prior to the establishment of flat-slab subduction. Two compositionally distinct samples of the Huayllapa pluton yielded mean ages of 24.8 ± 0.4 Ma and 25.4 ± 0.8 Ma. In contrast, the Cordillera Blanca batholith has a protracted crystallization history postdating that of the Cordillera Huayhuash by up to 20 m.y. Miocene magmatism in the Cordillera Blanca began at 19 Ma and ended with injection of large volumes of geochemically distinct, mantle-derived magma from 10 to 5 Ma. We suggest that 6–5 Ma magmatism in the Cordillera Blanca promoted elevated heat flow and reduced shear strength, which facilitated extensional shearing along the western slopes of the range, whereas colder amagmatic crust in the Cordillera Huayhuash inhibited southward propagation of faulting. Our data demonstrate that the linkages between magmatism and elevated heat flow identified in the Cordillera Blanca are important driving processes in initiating extension in cordilleran-style orogenies.

Eating through time: Understanding dietary practices across late prehistory in the northeastern Iberian Peninsula.

This study seeks to contribute to the current understanding of dietary variation in the late Prehistory of the northeastern Iberian Peninsula by examining buccal dental microwear patterns alongside archeological data from the same populations. Teeth from 84 adult individuals from eight distinct samples spanning the Middle-Late Neolithic to the Middle Bronze Age (Cova de l'Avi, Cova de Can Sadurní, Cova de la Guineu, Cova Foradada, Cova del Trader, Roc de les Orenetes, Cova del Gegant, Cova dels Galls Carboners) were analyzed using optical microscopy to examine buccal dental microwear patterns. The analysis did not reveal clear chronological contrasts in the dietary habits of these samples. Nevertheless, significant differences emerged among the samples, leading to their classification into two distinct sets based on the abrasiveness of the diet informed by the microwear patterns. These findings offer similarities and differences among samples in the Iberian Peninsula, shedding light on the diverse lifestyles of these individuals. Integrating our new results with other available proxies points to a multifaceted specialization in dietary patterns among these samples, influenced by factors such as habitat, resource selection, and available technology. By contextualizing the results within the broader context of the Iberian Peninsula, this research discerns shared characteristics and distinctive adaptations in the dietary practices and subsistence strategies of these groups. Ultimately, this study contributes to a deeper understanding of the intricate interplay between culture and environment in shaping human diets throughout late Prehistory.

Proteomic analysis of Neobenedenia sp. and Rhabdosynochus viridisi (Monogenea, Monopisthocotylea): Insights into potential vaccine targets and diagnostic markers for finfish aquaculture

Monogeneans are parasitic flatworms that represent a significant threat to the aquaculture industry. Species like Neobenedenia melleni (Capsalidae) and Rhabdosynochus viridisi (Diplectanidae) have been identified as causing diseases in farmed fish. In the past years, molecular research on monogeneans of the subclass Monopisthocotylea has focused on the generation of genomic and transcriptomic information and the identification in silico of some protein families of veterinary interest. Proteomic analysis has been suggested as a powerful tool to investigate proteins in parasites and identify potential targets for vaccine development and diagnosis. To date, the proteomic dataset for monogeneans has been restricted to a species of the subclass Polyopisthocotylea, while in monopisthocotyleans there is no proteomic data. In this study, we present the first proteomic data on two monopisthocotylean species, Neobenedenia sp. and R. viridisi, obtained from three distinct sample types: tissue, excretory-secretory products (ESPs), and eggs. A total of 1691 and 1846 expressed proteins were identified in Neobenedenia sp. and R. viridisi, respectively. The actin family was the largest protein family, followed by the tubulin family and the heat shock protein 70 (HSP70) family. We focused mainly on ESPs because they are important to modulate the host immune system. We identified proteins of the actin, tubulin, HSP70 and HSP90 families in both tissue and ESPs, which have been recognized for their antigenic activities in parasitic flatworms. Furthermore, our study uncovered the presence of proteins within ESPs, such as annexin, calcium-binding protein, fructose bisphosphate aldolase, glutamate dehydrogenase, myoferlin, and paramyosin, that are targets for immunodiagnostic and vaccine development and hold paramount relevance in veterinary medicine. This study expands our knowledge of monogeneans and identified proteins that, in other platyhelminths are potential targets for vaccines and drug discovery.

Rapid Investigation of Oil Pollution in Water-Combined Induced Fluorescence and Random Sample Consensus Algorithm

The global issue of oil spreading in water poses a significant environmental challenge, emphasizing the critical need for the accurate determination and monitoring of oil content in aquatic environments to ensure sustainable development of the environment. However, the complexity arises from challenges such as oil dispersion, clustering, and non-uniform distribution, making it difficult to obtain real-time oil concentration data. This paper introduces a sophisticated system for acquiring induced fluorescence spectra specifically designed for the quantitative analysis of oil pollutants. The paper involved measuring the fluorescence spectra across 20 concentration gradients (ranging from 0 to 1000 mg/L) for four distinct oil samples: 92# Gasoline, Mobil Motor Oil 20w-40, Shell 10w-40 engine oil, and Soybean Oil. The research focused on establishing a relationship model between relative fluorescence intensity and concentration, determined at the optimal excitation wavelength, utilizing the segmented Random Sample Consensus (RANSAC) algorithm. Evaluation metrics, including standard addition recovery, average recovery, relative error, and average relative error, were employed to assess the accuracy of the proposed model. The experimental findings suggest that the average recovery rates for the four samples ranged between 99.61% and 101.15%, with the average relative errors falling within the range of 2.04% to 3.14%. These results underscore the accuracy and efficacy of the detection methodology presented in this paper. Importantly, this accuracy extends to scenarios involving heavier oil pollution. This paper exhibits exceptional sensitivity, enabling precise detection of diverse oil spills within the concentration range of 0~1000 mg/L in water bodies, offering valuable insights for water quality monitoring and sustainable development of the environment.

Exploring bar fractions: a comparative study of galaxy pairs and single galaxies

We present a comprehensive analysis of barred galaxies within two distinct samples: isolated single galaxies (SIG) and isolated galaxy pairs (SIP), drawn from the SDSS-based catalog of isolated galaxies and the catalog of isolated galaxy pair limited to M ≤ −18.5, respectively. Our primary aim is to investigate the influence of tidal effects on the bar fraction (f bar ) across various galaxy systems. Barred galaxies are identified through automated ellipse fitting analysis supplemented by visual inspection of r-band imaging. A strong correlation between f bar and both galaxy morphological type and star formation rate has been revealed. Although, barred galaxies represent a nearly equal percentage of 52% in SIG and 51% in SIP, we found a little evidence for the tidal dependence of bar formation where a notable increase in the f bar is observed among galaxies characterized by larger projected separation and higher radial velocity difference between the pair members. In SIGs, we observe a prevalent increasing trend of f bar with galaxy mass, contrasting the observed decrease in f bar with increasing galaxy mass in SIPs. This discrepancy is consistent with typical trends of weaker bars, suggesting that tidal effect may attenuate the strength of bar structures within galaxy pairs.

Impact of rainfall on the water quality of a tropical river: based on the Nilwala River in the southern province of Sri Lanka between March and October 2019

ABSTRACT The study addresses the pressing need for an effective monitoring system to assess aquatic water quality, given the exacerbating impact of climate change on surface water bodies. Focusing on the Nilwala River, the research pursues three main objectives: comparative analysis of the Weighted Arithmetic Water Quality Index (WAWQI) values across different river locations, exploration of correlations between monthly rainfall and various physicochemical parameters at distinct sampling sites, and examination of notable disparities in the WAWQI between dry and wet months. Data collected from eight river locations from March to October 2019 revealed significant temporal variations in pH, temperature, chemical oxygen demand (COD), electrical conductivity (EC), alkalinity, chloride (Cl−), and nitrate (NO3-) content. The WAWQI increased across all sites during the drought, with only Site 4 (Wellathota) deemed suitable for drinking. Statistical analyses using an one-way ANOVA and multiple linear regressions unveiled significant relationships between rainfall and pH, biological oxygen demand (BOD), EC, and total alkalinity. The total alkalinity is the most affected parameter by the rainfall. Rainfall positively predicted BOD, COD, and NO3- concentration while negatively predicting other parameters.

Ecological observations of freshwater snails in the vicinity of an artificial lake

This investigation delves into the ecological dimensions of freshwater snails in the vicinity of an artificially created lake. The aim of this research was to comprehend the distribution, prevalence, and variety of freshwater snail species within the ecosystem surrounding the manmade lake. The study spanned from August to November 2023, conducted at three distinct sample stations (I, II, and III), with bi-weekly sample collections. Standard keys were employed for the identification of snails, and the Shanon-Weiner and Simpson's similarity indices were used to explore the diversity of snail species. Throughout the study, a total of 311 individuals representing three distinct species encountered in this study were documented. Acathina fulica exhibited the highest abundance (129 individuals), while Pomacea bridgesii displayed the lowest abundance (66 individuals). The month of August registered the highest population (122 individuals), contrasting with the lowest count in November (37 individuals). Abundance varied across stations, with Station II recording the lowest (77 individuals) and Station III the highest (116 individuals). Regarding diversity, Pomacea bridgesii emerged as the most diverse species (0.3670 & 0.3662), while Littorina littorea exhibited the least diversity (0.0000) across the stations. This investigation contributes valuable insights into the dynamics of freshwater snail populations in the periphery of artificial lakes, shedding light on their distribution patterns and ecological interactions.

Abstract PO5-04-05: Clinical validation of the concordance performance of ERBB2 status by single-cells genomics ERBB2 (HER2) amplification Assay in Circulating Tumor Cells (CTCs) from patients with matched HER2 status from metastatic tissue biopsies

Abstract Background Liquid biopsies are a non-invasive diagnostic approach for detecting CTCs that may provide clinically actionable information for treatment decisions for metastatic breast cancer (MBC) patients when a conventional biopsy is infeasible. Blood-based liquid biopsy has the potential advantage that it allows broad sampling of metastatic tumor clones because it is capable of and likely to obtain cellular and genomic contributions from more than one metastatic site. Here we report the concordance performance of HER2 positivity by single-cell CTC genomics ERBB2 (HER2) amplification in patients with matched HER2 status from metastatic tissue biopsies. Methods A prospective Clinical Experience Program for CTC ERBB2 (HER2) Assay across 25 different United States-based cancer centers, was used to recruit 128 MBC patients with documented standard-of-care metastatic tissue biopsies performed within the last 5 years (Days from tissue biopsy to liquid biopsy, Median: 19.5; interquartile range: 40.75) CTCs from blood collection at each respective clinical site were isolated from plasma, nucleated cells were plated, & slides were bio-banked for Immunofluorescent staining & subsequent imaging in a CAP CLIA lab. CTCs were identified using Epic Sciences digital imaging & machine learning algorithms. Individual CTCs were sequenced for genomic quantification of chromosomal instability (LSTs) and ERBB2 (HER2) amplification. Results Within the clinical validation cohort of 128 MBC patients, 30% (38/128) were HER2-positive by standard pathologic criteria (IHC 3+ or IHC2+/ISH+) in metastatic tissue biopsies. Among the 64 out of 128 cases with reportable results by the CTC ERBB2 (HER2) amplification on chromosomally unstable CTCs, 31% (20/64) of patients had ERBB2 amplification. Concordance between CTC single cell genomics and available tissue results for HER2 positivity showed a sensitivity of 69% and specificity of 78%. We then addressed the effects of two known biological confounders to the use of tissue biopsy for HER2 status determination: 1) tissue biopsies performed on bone metastases, 2) tumor evolution driven by treatment pressure over time leading to potential HER2 conversion and tumor heterogeneity. Thus, we performed subgroup analysis including only validation set patients with tissue biopsies performed on non-bone tissues, which showed slightly improved concordance to the tissue comparator with a Sensitivity of 86%, and Specificity of 75%. Next, to exclude the possibility of tumor evolution we selected only patients with the HER2 status comparator performed on non-bone tissue biopsies contemporaneous (Days from tissue biopsy to liquid biopsy, Median: 14; interquartile range: 22) to the blood draw for the CTC ERBB2 (HER2) amplification assay. This showed dramatically improved concordance performance with a Sensitivity of 100%, and a Specificity of 75%. Conclusions Blood and tissue are two biologically distinct sample types where variations in HER2 and other biomarker results might be expected due to analytic, temporal and clinical differences. These data indicate that determination of HER2 status by single cell genomic ERBB2 (HER2) amplification assay can aid in the clinical assessment of ERBB2 status in patients with metastatic breast cancer (MBC) for whom tissue biopsy for HER2 status is not available, or infeasible. Additionally, these results provide evidence of the contribution of site differences (bone) of metastatic biopsies and also tumor evolution to discordance between the CTC ERBB2 (HER2) Assay and tumor biopsy. Citation Format: Giuseppe Di Caro, Ernest Lam, Megan Slade, Shuguang Huang, Rick Wenstrup, Lee Schwartzberg. Clinical validation of the concordance performance of ERBB2 status by single-cells genomics ERBB2 (HER2) amplification Assay in Circulating Tumor Cells (CTCs) from patients with matched HER2 status from metastatic tissue biopsies [abstract]. In: Proceedings of the 2023 San Antonio Breast Cancer Symposium; 2023 Dec 5-9; San Antonio, TX. Philadelphia (PA): AACR; Cancer Res 2024;84(9 Suppl):Abstract nr PO5-04-05.

SERUM CALCIUM IS ASSOCIATED WITH PERIPHERAL AND CENTRAL BLOOD PRESSURE IN DIFFERENT SAMPLES

Objective: Calcium homeostasis plays a crucial role in both contractility and relaxation mechanisms, determining the vasoconstriction and vasodilation of blood vessels. Some studies have reported a positive correlation between serum calcium and blood pressure (BP) levels, hypertension, metabolic syndrome, and type 2 diabetes, while others have not. Given these conflicting findings, we investigated the relationship between serum calcium and BP in three distinct samples. Our objective was to confirm the existence of a positive correlation between calcium levels and BP and to assess whether this relationship extends to central BP. Design and method: Serum calcium levels were measured in a restricted sample of patients referred to our ESH Excellence Center in Verona (n=56) with a suspicion of secondary hypertension, as well as in two large population-based studies from Sweden, the Malmö Preventive Project (MPP, n= 17,843) and from United Kingdom, the UK Biobank (UKB, n= 330,000). Central BP was estimated by tonometry performed with SphygmoCor Xcel. Results: In the Verona sample, the patients, predominantly receiving antihypertensive therapy based on calcium antagonists, serum calcium correlated with central systolic BP (r=0.37; p<0.01), and this association persisted even after adjusting for age, sex, and BMI. In the MPP, baseline serum calcium was associated with both systolic (beta 0.60, 95% CI 0.30-0.89; p<0.001) and diastolic BP (beta 0.44, 95% CI 0.26-0.62; p<0.001) after adjustment for age, sex, BMI, and antihypertensive therapy. Similarly, in the UKB, the association between both systolic (beta 1.79, 95% CI 1.74-1.85; p<10^15) and diastolic BP (beta 0.98, 95% CI 0.95-1.02; p<10 ^15) and serum calcium was confirmed after the same adjustments. Conclusions: In different samples, serum calcium consistently associates with increased BP. Further studies are warranted to elucidate whether this association is influenced by hormones regulating this electrolyte and/or by dietary calcium intake.

Evaluation of the Posttraumatic Stress Disorder Checklist for DSM-5 across United States and India samples: An item response theory analysis using the graded response model.

The Posttraumatic Stress Disorder (PTSD) Checklist for DSM-5 (PCL-5) is frequently used to assess PTSD symptoms. Extending its psychometric investigations across distinct samples (United States and India), the aims of the present study included investigating the item characteristics, person fit, and differential item functioning (DIF) across the two samples. We (a) conducted item analysis using the graded response model to examine item characteristics (thresholds and discrimination parameters) and (b) examined person fit to determine participants' response patterns. The U.S. sample included 176 trauma-exposed individuals seeking mental health treatment, and the Indian sample included 148 trauma-exposed ex-military personnel. Results indicated low discrimination for Item 8 and low but acceptable discrimination for Item 17 for the U.S. and Indian samples, respectively. Across both samples, the most unused response categories were the middle-point or extreme (higher severity) categories (all response categories were better utilized in the Indian sample), and half the participants exhibited person misfit. Supplemental DIF analysis indicated that five items exhibited DIF indicating potential item bias; however, effect sizes for DIF were negligible. Although the PCL-5 demonstrated strong psychometric properties and showed promise for use across the samples differing on cultural and demographic characteristics, some of the items and the number of categories used to measure them could be revisited to create a more efficient instrument. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Cosmic insights from galaxy clusters: Exploring magnification bias on sub-millimetre galaxies

Context. Magnification bias, an observational effect of gravitational lensing in the weak regime, allows the cosmological model to be tested through angular correlations of sources at different redshifts. This effect has been observed in various contexts, particularly with sub-millimetre galaxies (SMGs), offering valuable astrophysical and cosmological insights. Aims. The study aims to investigate the magnification bias effect exerted by galaxy clusters on SMGs and its implications for astrophysical and cosmological parameters within the Λ-CDM model. Methods. Magnification bias was explored by quantifying the cross-correlation function, which we then utilised to derive constraints on cosmological and astrophysical parameters with a Markov chain Monte Carlo algorithm. Two distinct galaxy cluster samples were used to assess result robustness and understand the influence of sample characteristics. Results. Cluster samples show higher cross-correlation values than galaxies, with an excess at larger scales suggesting contributions from additional large-scale structures. The parameters obtained, while consistent with those of galaxies, are less constrained due to broader redshift distributions and limited cluster statistics. Results align with weak lensing studies, hinting at slightly lower σ8 and Ωm values than Planck’s cosmic microwave background data, emphasising the need for enhanced precision and alternative low-redshift universe tests. Conclusions. While this method yields constraints that are compatible with the Λ-CDM model, its limitations include broader redshift distributions and a limited number of lenses, resulting in less constrained parameters compared to previous galaxy studies. Nonetheless, our study underscores the potential of using galaxy clusters as lenses for magnification bias studies, capitalising on their elevated masses and thus providing a promising avenue to test current cosmology theories. Further progress can be made by expanding the lens sample size.

Effects of Cissus quadrangularis L. Powder on Proximate Composition, Physicochemical and Textural Properties of Tteokgalbi.

We investigated Cissus quadrangularis L. powder (C) use as a natural additive to Tteokgalbi, a traditional Korean meat-based dish. Five distinct Tteokgalbi samples were treated: one without any additives (negative control, NC), one with 1.00% C (C1), 2.00% C (C2), 4.00% C (C3), and 0.10% ascorbic acid (positive control, PC). C addition resulted in changes in composition, quality, and sensory attributes. Moisture content decreased with higher C levels; crude protein varied among the groups, with C1 having the highest crude protein levels and C3 the lowest. Crude fat decreased with increasing C concentration, whereas the carbohydrate content increased. The water-holding capacity notably decreased in the C3 group, resulting in increased cooking loss with higher C concentrations. C treatment altered color and texture, reducing CIE L* and increasing CIE a* before cooking and increasing CIE L* and CIE a* after cooking. CIE b* decreased before cooking but increased thereafter. C-treated Tteokgalbi was less cohesive, chewy, and brittle compared to the NC. The C treatment increased the total phenolic and flavonoid contents and enhanced radical scavenging capacities. It also affects storage characteristics, lowers pH, and increases 2-thiobarbituric acid reactive substances values. The microbial counts were lower in C2 and C3 after 11 days. These findings suggest the potential use of C as a natural meat additive.

Time of day does not impact spinal serotonin levels in humans.

Spinal serotonin enables neuro-motor recovery (i.e., plasticity) in patients with debilitating paralysis. While there exists time of day fluctuations in serotonin-dependent spinal plasticity, it is unknown, in humans, whether this is due to dynamic changes in spinal serotonin levels or downstream signaling processes. The primary objective of this study was to determine if time of day variations in spinal serotonin levels exists in humans. To assess this, intrathecal drains were placed in seven adults with cerebrospinal fluid (CSF) collected at diurnal (05:00 to 07:00) and nocturnal (17:00 to 19:00) intervals. High performance liquid chromatography with mass spectrometry was used to quantify CSF serotonin levels with comparisons being made using univariate analysis. From the 7 adult patients, 21 distinct CSF samples were collected: 9 during the diurnal interval and 12 during nocturnal. Diurnal CSF samples demonstrated an average serotonin level of 216.6 67.7nM. Nocturnal CSF samples demonstrated an average serotonin level of 206.7 75.8nM. There was no significant difference between diurnal and nocturnal CSF serotonin levels (p =.762). Within this small cohort of spine healthy adults, there were no differences in diurnal versus nocturnal spinal serotonin levels. These observations exclude spinal serotonin levels as the etiology for time of day fluctuations in serotonin-dependent spinal plasticity expression.

Quantitative Analysis of Caffeine and Phosphoric Acid in Non-Alcoholic Beverages Marketed in Kosovo

Non-alcoholic beverages often contain various constituents and additives, influencing both their nutritional profiles and functional attributes. Given the ubiquity of these beverages and their potential health implications, rigorous quality checks are indispensable to ascertain compliance with health and safety standards. This research aimed to quantify caffeine and phosphoric acid levels in a diverse array of soft drinks from Kosovo’s market. To achieve this, 41 distinct non-alcoholic beverage samples from the local market were scrutinized. Caffeine concentrations were determined via UV-Vis spectrophotometry, while potentiometric titration was employed to assess phosphoric acid levels. As expected, energy drinks in Kosovo contained markedly higher caffeine concentrations compared to carbonated soft drinks and ice-tea variants. On the other hand, based on the results, analysed energy drinks showed somewhat greater caffeine contents than those reported in packaging of the beverage samples. Our study showed that a significant portion of the samples (21.95% for caffeine and 9.76% for phosphoric acid) did not conform to the standards set by EU 1169/2011 regulation or the EU 1333/2008 regulation. These findings underscore the urgency for relevant food safety authorities to implement rigorous oversight and enact appropriate protective measures. It is also imperative for the Kosovo National Food Safety Authority to craft specific regulations stipulating permissible additive concentrations, especially for caffeine and phosphoric acid, in non-alcoholic beverages.

Effect of Incorporating Chitosan to Resin Modified Glass Ionomer Cement on Shear Bond Strength to Dentin (An In vitro Comparative Study).

Resin-modified glass ionomer cement tends to shrink due to polymerization of the resin component. Additionally, they are more prone to syneresis and imbibition during the setting process. This in vitro study evaluates the impact of chitosan, a biopolymer that is, both biomaterial and biocompatible, on the strength of dentin bonding and compares it with ACTIVA Bio-ACTIVE Restorative. The present study was aimed to assess the impact of including chitosan into Fuji II on the shear bond strength between. the restoration material and tooth dentin, in contrast to Bioactiva in permanent teeth. A total of 30 premolar teeth were recently extracted. The study involved three distinct sample groups. Group 1 (10 teeth) is the negative control (Fuji II), Group 2 (10 teeth) is the positive control (ACTIVA Bio-ACTIVE Restorative), and Group 3 (10 teeth) is treated with a mixture of Chitosan and Fuji II (CH-Fuji II). Each tooth's buccal and palatal cusps were eliminated to achieve a horizontal surface. Using a periodontal probe, 1.5 mm from the mesial pit to the mesial marginal ridge were removed. Restoration was implemented in all groups following manufacturer directions. Thermocycling the teeth by immersing them in a water bath with temperatures ranging from 5°C to 55°C (± 1-2°C) for 30 s (500 cycles). Each sample was attached to the universal testing machine's jig at a cross-head speed of 1 mm/min. Shear force was used until breakage, and the bond's adhesive strength was then calculated. Statistical analysis using ANOVA with Dunnett's T3 post hoc test. Results were significant at P < 0.05. Statistically significant difference was present between Chitosan and Fuji II and between Chitosan and Activa by reducing the shear bond strength. Addition of chitosan to Fuji II had a negative effect on the shear bond with a significant difference while Activa and Fuji II exhibited favorable shear bond strength.

Effect of Tin Addition on the Mechanical Properties and Microstructure of Aluminium Bronze Alloyed with 4% Nickel

The current study investigates the impact of adding tin to aluminium bronze alloyed with 4% nickel on its microstructure and mechanical properties. Sand casting was chosen as the most cost-effective and efficient method of preparing the aluminium bronze alloy. Following their melting points, six distinct samples of aluminium bronze alloyed with 0% to 10% tin were added into the crucible furnace. Nickel with the highest melting point of 1453°C was added into the crucible furnace first, while tin with the lowest melting of 231.9°C was added last into the crucible furnace. The alloying components were mixed well by manually mixing the liquid for around five minutes. After sand casting, the specimens were machined, sectioned, and grounded then tests were carried out to measure their hardness, tensile strength, and impact resistance. The results of the tests indicate that the tensile strength first increases and subsequently declines as the tin addition increases. The hardness of the aluminium bronze alloy increases as the proportion of tin addition increases. The results of the investigations also demonstrate that as the hardness of the specimens increases, their impact resistance decreases and the tensile stress of each specimen increases with strain.

Advancing mechanical durability and radiation shielding properties in Silicon dioxide (SiO2) glasses through various incorporations: A comparative analysis

Silicon dioxide (SiO2) glasses, known for their high thermal stability, excellent optical transparency, and substantial mechanical strength, are crucial in numerous technological applications, including radiation shielding. This research explores the impact of compositional variations in SiO2-based glasses on their mechanical and radiation shielding properties, particularly focusing on the inclusion of heavy metal oxides (HMO) and rare earth elements (REE) like neodymium (Nd). Through the systematic investigation of fifteen distinct glass samples with varying concentrations of specific oxides and elements, we investigate the compositional changes and their influence on physical properties and their effectiveness in attenuating radiation. Our findings demonstrate that the incorporation of Nd significantly enhances the glass's radiation shielding capabilities. Glasses doped with Nd exhibited higher effective atomic numbers and electron densities, which translate to superior attenuation characteristics at lower photon energies. This is highlighted by the exceptional performance of the 20Nd sample, showing the lowest exposure build-up factors (EBF) at 10 mean free paths (mfp), indicating its potential as a premier candidate for shielding applications against various energy levels of radiation. Moreover, the variation in the Elastic Modulus of the glass samples underscores the significant impact of the glass matrix composition on its mechanical properties, suggesting a delicate balance between network formers and modifiers in determining the glass properties. It can be concluded that the neodymium-doped SiO2-based glasses may be considered as targeted compositions in fine-tuning material properties to meet specific application requirements. As the demand for efficient radiation shielding materials grows across medical, industrial, and space exploration sectors, our findings provide a solid foundation for the development of new glass formulations tailored for enhanced mechanical properties and superior radiation protection levels.