Distinct Notch Research Articles

INVESTIGATION OF BROADER BANDWIDTH ELLIPTICAL DIPOLE ANTENNA PERFORMANCE PARAMETERS FOR 5G, KU AND KA-BAND APPLICATIONS: DIELECTRIC CONSTANT EFFECTS

This study presents an examination of the design and evaluation process of an elliptical dipole antenna operating at the design frequency of 5 GHz. Initially based on conventional dipole antenna principles, the antenna's design is iteratively refined through bandwidth enhancement techniques, resulting in the emergence of ultra-wideband characteristics. Notably, the modified antenna design features a distinctive notch band spanning from 4.21 to 10.06 GHz, positioning it as a versatile solution suitable for applications across the mid-band of 5G, as well as the full Ku and partial Ka bands, encompassing frequencies from 27 GHz to 31 GHz. Furthermore, this study investigates the impact of varying dielectric constants on antenna parameters, shedding light on a critical aspect often overlooked in conventional dipole antenna calculations. Through systematic analysis, it is revealed that a dielectric constant of 4.3, aligned with the commonly used low-cost epoxy-based FR-4 material, yields optimal performance. While higher dielectric constants broaden the antenna's characteristics, lower values are found to enhance communication efficiency, thus offering potential benefits in terms of power consumption and cost savings. The detailed examination and findings presented in this study enrich the understanding of antenna design principles and provide valuable guidance for future research and practical applications in the field of wireless communication and antenna engineering.

Shape variation and sex differences of the adult human mandible evaluated by geometric morphometrics

In cases of osseous defects, knowledge of the anatomy, and its age and sex-related variations, is essential for reconstruction of normal morphology. Here, we aimed at creating a 3D atlas of the human mandible in an adult sample using dense landmarking and geometric morphometrics. We segmented 50 male and 50 female mandibular surfaces from CBCT images (age range: 18.9–73.7 years). Nine fixed landmarks and 510 sliding semilandmarks were digitized on the mandibular surface, and then slid by minimizing bending energy against the average shape. Principal component analysis extracted the main patterns of shape variation. Sexes were compared with permutation tests and allometry was assessed by regressing on the log of the centroid size. Almost 49 percent of shape variation was described by the first three principal components. Shape variation was related to width, height and length proportions, variation of the angle between ramus and corpus, height of the coronoid process and inclination of the symphysis. Significant sex differences were detected, both in size and shape. Males were larger than females, had a higher ramus, more pronounced gonial angle, larger inter-gonial width, and more distinct antegonial notch. Accuracy of sexing based on the first two principal components in form space was 91 percent. The degree of edentulism was weakly related to mandibular shape. Age effects were not significant. The resulting atlas provides a dense description of mandibular form that can be used clinically as a guide for planning surgical reconstruction.

Abstract 777: Notch signaling dependent chemoresistance in racial disparity in ER+ breast cancer

Abstract Breast cancer (BCa) is the leading cause of cancer related death in women worldwide. The most common BCa subtype is the Estrogen/Progesterone receptor (ER+/PR+) luminal subtype, comprising up to 75% of all newly diagnosed BCa. Although these patients show initial benefit with chemotherapy and endocrine therapy, however, 40-50% of them eventually don’t respond to treatment due to resistance. Thus, the need for additional targeted therapies for this large subset of ER+ BCa patients is critical. Based on recent studies, it is observed that Afro-American (AA) patients experience substantially higher BCa mortality (40% more) than White American (WA) women in ER+ breast cancer. Unfortunately, the cause behind this difference in mortality in AA patients is not well understood. Notch signaling is a highly conserved intercellular communication mechanism critical for many cellular processes and is composed of several receptors and ligands. Recent data show that Notch receptors are overexpressed in hormone treatment refractory ER− patients of (Afro-American) AA origin, hinting connection of Notch signaling to racial disparity in breast cancer. Notch signaling is also identified in several metabolic disorders involving fatty acids and other lipids. However, function of Notch signaling in context of chemoresistance in AA ER+ breast cancer is still unknown. Using single cell sequencing, we found that chemo-resistant AA ER+ breast cancer patient samples have a distinct Notch signature enriched subpopulations in tumor cells, which was missing in WA patients. Notch signaling happens through juxtacrine signaling of ligand/receptor signaling. Further analysis in tumor microenvironment shows lipid enriched tumor associated macrophages, which are Notch dependent, suggesting potential crosstalk between lipid heavy tumor-macrophages and tumor cells, which may drive the chemoresistance in AA patients. Further analysis using patient derived explants, an attractive new tool for drug efficacy testing in ex vivo, we found that chemotherapy itself increases Notch signaling in the tumors. Ongoing studies involving scRNAseq, untargeted lipidomics and chemo-resistant PDE will delineate the precise mechanism of action between tumor cells and tumor associated lipid heavy macrophages in context of Notch signaling in chemo-resistant AA ER+ breast cancer. Citation Format: Shailesh Singh, Megha Das, Rumela Chakrabarti. Notch signaling dependent chemoresistance in racial disparity in ER+ breast cancer [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 777.

Ultrathin Platinum Film Hydrogen Sensors with a Twin-T Type Notch Filter Circuit.

In recent years, hydrogen energy has garnered attention as a potential solution for mitigating greenhouse gas emissions. However, concerns regarding the inherent risk of hydrogen gas leakage and potential explosions have necessitated the development of advanced sensors. Within our research group, we have innovated an ultrathin platinum (Pt) film hydrogen sensor that gauges resistance changes in Pt thin films when exposed to hydrogen gas. Notably, the sensitivity of each sensor is contingent upon the thickness of the Pt film. To address the challenge of detecting hydrogen using multiple sensors, we integrated the ultrathin Pt film as a resistance element within a twin-T type notch filter. This filter exhibits a distinctive reduction in output signals at a specific frequency. The frequency properties of the notch filter dynamically alter with changes in the resistance of the Pt film induced by hydrogen exposure. Consequently, the ultrathin Pt film hydrogen sensor monitors output signal variations around the notch frequency, responding to shifts in frequency properties. This innovative approach enables the electrical control of sensor sensitivity by adjusting the operating frequency in proximity to the notch frequency. Additionally, the simultaneous detection of hydrogen by multiple sensors was successfully achieved by interconnecting sensors with distinct notch frequencies in series.

Finite Element Analysis of Notch Depth and Angle in Notch Shear Cutting of Stainless-Steel Sheet

Piercing is a crucial process in the sheet metal forming industry, and the surface quality of the pierced part is an important parameter that defines the overall quality of the product. However, obtaining a good surface quality is a challenging task that depends on the effect of several process parameters and necessitates the use of non-conventional procedures. Notch shear cutting is a relatively new progressive approach in which the workpiece is indented with a notch form with a predefined notch angle and depth, and then the indented workpiece is subjected to conventional piercing. In this study, conventional piercing and notch shear cutting processes were experimentally performed on 1.4301 stainless steel sheet of 2 mm thickness. Then, finite element (FE) analyses were conducted utilizing Deform-2D software. After ensuring that the experimental and simulation works were consistent with each other, the FE analysis of notch shear cutting was carried out for three distinct notch depths (15 %, 30 %, and 60 % of the workpiece thickness) and six different notch angles (10º, 20º, 30º, 40º, 50º, and 60º). Investigations were performed on shear zone length distributions, which are direct indications of the surface quality on sheared workpieces, crack propagation angles, and required cutting loads. The best surface quality was obtained when the notch angle was set to 50º and the notch depth was set to 15 % of the workpiece thickness. It was also observed that notch angle and notch depth had a certain level of influence on required cutting load.

EPEN-11. CHARACTERIZATION OF THE SPATIAL ARCHITECTURE OF INTRATUMORAL CELL STATES IN PEDIATRIC HIGH-RISK EPENDYMOMA

Abstract By single-cell analyses, we previously uncovered distinct intratumoral transcriptomic ependymoma (EPN) cell states and developmental trajectories correlating with molecular subtype and biological behavior. However, the relation of these cell states to characteristic EPN tissue architecture and tumor microenvironment remains widely unknown. We aimed to characterize the spatial architecture and elucidate regulatory circuits in EPN via spatial transcriptomics (10X Visium) of tumor specimens matching our previously generated single-cell transcriptome data spanning all EPN subtypes (ZFTA, YAP1, PFA, PFB). We investigated whether spatially restricted cell-cell interactions correlate with our previously identified cell states. Analyses revealed distinct spatially resolved cell populations in supratentorial and posterior fossa EPN. All subgroups showed several (5-9) spatially discrete cell clusters. Via non-negative matrix factorization (NMF), cell-specific spatial clusters emerged correlating with previous single-cell programs. ZFTA EPN highly correlates with ZFTA-specific metaprograms whereas all other subgroups did not show ZFTA-specific gene expression. Explicit genes such as DLK1 are exclusively upregulated in ZFTA EPN indicating spatially distinct aberrant Notch pathway signaling. Interestingly, this subgroup revealed spatial zones with upregulation of metabolic genes and endothelial markers anti-correlating with a ZFTA-specific program. Ependymal and neuronal-precursor-like signatures were enriched in our unique YAP1 EPN sample in a spatially restricted pattern, whereas GFAP was ubiquitously expressed. PFA was characterized by a neural stem cell (NSC)-like niche accompanied by metabolic and immune-reactive gene signatures. PFB showed a similar cell niche associated with NSC-like and glial-progenitor-like genes enriched simultaneously, further highlighting the NSC characteristic of posterior fossa tumors. Ependymal signatures were absent in PFA and present in PFB indicating more differentiated cell populations in PFB. Summarizing, we confirm presence of distinct intratumoral cell states within ependymoma tissue and reveal their spatial organization. In depth analysis and functional validation in matched ependymoma models will enable interrogation of underlying tumor promoting signaling events driving intratumoral developmental trajectories.

Two New Species of Snailfishes (Cottiformes: Liparidae) from the Aleutian Islands, Alaska, and a Redescription of the Closely Related Careproctus candidus

Two new species of snailfishes are described from the Aleutian Islands. Both are similar to Careproctus candidus, described originally from four specimens, here redescribed on the basis of 67 specimens in addition to the types. All three species are allocated to the Careproctus subgenus Temnocora. Careproctus candidus is distinguished from all other species of Careproctus by the combination of an anterior dorsal-fin lobe formed by a distinct notch, pupil a horizontal slit, and variegated reddish coloration. It is redescribed to note the presence of prickles, a variable pectoral-girdle morphology, and variable exsertions of the fin rays of the dorsal-fin lobe. Careproctus klisi, new species, is distinguished by having an anterior dorsal fin uniquely of five to seven short anterior dorsal-fin rays nearly equal in length preceding distinctly longer succeeding rays, pupil a horizontal slit, a variegated reddish coloration, and a relatively deep body. Careproctus bromius, new species, is distinguished by having low vertebral and median-fin ray counts, pupil a horizontal slit, a variegated light brown and pale coloration, and in lacking an anterior dorsal-fin lobe. Careproctus candidus ranges across the Aleutian Islands from Attu Island to the northern Gulf of Alaska at depths from 88 to 432 m. Careproctus klisi, new species, is found in the central and western Aleutian Islands from off Buldir Island in the west to Amlia Island in the east at depths from 134 to 180 m. Careproctus bromius, new species, is found in the central and eastern Aleutian Islands from Amchitka Pass to off Akutan Island at depths from 80 to 232 m.

Reencounter with the past: occurrence of sei whale (Balaenoptera borealis) in an old hunting area in the south-eastern Pacific Ocean

The sei whale (Balaenoptera borealis) was intensively exploited throughout its range, with about 110.000 individuals hunted by pelagic fleets in Antarctic waters between 1960 and 1970. In addition, basic information on its distribution, migratory routes, and feeding grounds in the southeastern Pacific, has been poorly documented. In the case of Chile, recent information consists mainly of accidental records. This research presents the first sei whale photo-identification catalog for south-central Chile. From November 2019 to January 2020, 88 individuals were recorded from land-based and boat surveys at Caleta Chome. Of these, 12 individuals were photo-identified through scars or distinctive notches in the dorsal fins. The peak of sightings occurred during December 2019; two individuals were sighted on more than one occasion.

Estimating Population Abundance of Atlantic Bottlenose Dolphins (Tursiops truncatus) in the Coastal Waters of Palm Beach County, Southeastern Florida

Atlantic bottlenose dolphins (Tursiops truncatus)are frequently observed in the coastal waters of Palm Beach County, Florida. In this study, mark-recapture surveys using photo-identification techniques were used to estimate population abundance of Atlantic bottlenose dolphins in this area for the first time. Surveys were conducted for 11 years, between 2005 and 2016, during which 384 individuals were identified and catalogued from distinctive nicks and notches on their dorsal fins. Each marked individual was documented an average of 2.4 times (range 1 to 19). Low resighting rates and a constant rate of encounter with previously unmarked individuals suggest that the population is open, comprising both residents and transients. The Jolly-Seber method was used to estimate population abundance. Estimates of abundance varied greatly in time with an overall average of 264 individuals and a 95% confidence interval of 162 to 366 (SE = 51.3), indicating a variable population size. As threats in the region continue to rise, such as overfishing, toxic algae blooms, and environmental pollutants, this understudied population may be vulnerable to decline. A better understanding of the population dynamics could facilitate more effective conservation action.

Delineating cooperative effects of Notch and biomechanical signals on patterned liver differentiation

Controlled in vitro multicellular culture systems with defined biophysical microenvironment have been used to elucidate the role of Notch signaling in the spatiotemporal regulation of stem and progenitor cell differentiation. In addition, computational models incorporating features of Notch ligand-receptor interactions have provided important insights into Notch pathway signaling dynamics. However, the mechanistic relationship between Notch-mediated intercellular signaling and cooperative microenvironmental cues is less clear. Here, liver progenitor cell differentiation patterning was used as a model to systematically evaluate the complex interplay of cellular mechanics and Notch signaling along with identifying combinatorial mechanisms guiding progenitor fate. We present an integrated approach that pairs a computational intercellular signaling model with defined microscale culture configurations provided within a cell microarray platform. Specifically, the cell microarray-based experiments were used to validate and optimize parameters of the intercellular Notch signaling model. This model incorporated the experimentally established multicellular dimensions of the cellular microarray domains, mechanical stress-related activation parameters, and distinct Notch receptor-ligand interactions based on the roles of the Notch ligands Jagged-1 and Delta-like-1. Overall, these studies demonstrate the spatial control of mechanotransduction-associated components, key growth factor and Notch signaling interactions, and point towards a possible role of E-Cadherin in translating intercellular mechanical gradients to downstream Notch signaling.

Cavitas Glenoidalis Morfolojisinin Kuru Kemikler Üzerinde İncelenmesi

Aim: The aim of this study was to determine the notch and shape variations of the glenoid cavity (GC), to emphasize its clinical importance, and compare it with the previous studies. Material and Methods: This study was performed with 157 (78 right sides, 79 left sides) adult Anatolian dry scapulae. The GCs were typed as oval, pear, and inverted comma shaped and noted the number of GC notch. Results: The most common GC shape was found as the pear shaped with 63 (80.8%) at the right side, 49 (62%) at the left side, and 112 (71.4%) in total. The second most common GC shape was found as oval shaped with 13 (16.6%) at the right side, 28 (35.5%) at the left side, and 41 (26.1%) in total. The glenoid notch was found at the right side, left side, and totally; 28 (35.9%), 19 (24.1%), and 47 (29.9%), respectively. All of the inverted comma shaped GCs had distinct glenoid notch, while the oval shaped GCs not. The pear shaped GCs had indistinct glenoid notch or no glenoid notch. The glenoid notch was found at the right side, left side, and totally on the pear shaped GC; 26 (41.3%), 17 (34.7%), and 43 (38.4%), respectively. Conclusion: Pear shaped GC was found as the most (71.4%) common shape in this study. Forty-seven (29.9%) of the scapulae had a glenoid notch. The notch and shape variations of the GC are important and this study will contribute to anatomists, orthopedists, and radiologists from this perspective.

Temporal cell fate determination in the spinal cord is mediated by the duration of Notch signalling

During neural development, progenitor cells generate different types of neurons in specific time windows. Despite the characterisation of many of the transcription factor networks involved in these differentiation events, the mechanism behind their temporal regulation is poorly understood. To address this question, we studied the temporal differentiation of the simple lateral floor plate (LFP) domain in the zebrafish spinal cord. LFP progenitors generate both early-born Kolmer-Agduhr” (KA”) interneuron and late-born V3 interneuron populations. Analysis using a Notch signalling reporter demonstrates that these cell populations have distinct Notch signalling profiles. Not only do V3 progenitors receive higher total levels of Notch response, but they collect this response over a longer duration compared to KA” progenitors. To test whether the duration of Notch signalling determines the temporal cell fate specification, we combined a transgene that constitutively activates Notch signalling in the ventral spinal cord with a heat shock inducible Notch signalling terminator to switch off Notch response at any given time. Sustained Notch signalling results in expanded LFP progenitors while KA” and V3 interneurons fail to specify. Early termination of Notch signalling leads to exclusively KA” cell fate, despite the high level of Notch signalling, whereas late attenuation of Notch signalling drives only V3 cell fate. This suggests that the duration of Notch signalling, not simply the level, mediates cell fate specification. Interestingly, knockdown experiments reveal a role for the Notch ligand Jag2b in maintaining LFP progenitors and limiting their differentiation into KA” and V3 interneurons. Our results indicate that Notch signalling is required for neural progenitor maintenance while a specific attenuation timetable defines the fate of the postmitotic progeny.

A new species of sublittoral marine copepod, Normanella parvisetosa sp. nov. (Harpacticoida, Normanellidae), from the Southern Sea of South Korea

Abstract A new harpacticoid species, Normanella parvisetosa sp. nov., belonging to the family Normanellidae Lang, 1944, is described from subtidal habitats in the Southern Sea of South Korea. This new species is assigned to the minuta-lineage, based on the rostrum with a pointed apical tip, the five-segmented female antennule, the female P5 exopod without a distinct notch on the outer margin, and well-developed caudal setae IV and V each bearing an internal fracture plane. Normanella parvisetosa sp. nov. is morphologically close to N. incerta Lang, 1934, N. porosa Noodt, 1964, and N. obscura Lee & Huys, 1999, whereas the new species is distinguished from females of these species in the shape of the rostrum with a pointed tip, the relative length to width ratio of the caudal rami, the second endopodal segment of the second to fourth legs, the exopod of the fifth leg, and the relative length of setae on both rami of the fifth leg. Herein, we provide a detailed description and illustrations of Normanella parvisetosa sp. nov., which is the second Normanella species identified in Korean waters.

Two distinct Notch signals, Delta-like 4/Notch1 and Jagged-1/Notch2, antagonistically regulate chemical hepatocarcinogenesis in mice

Notch signaling is one of the most common drivers of carcinogenesis in many types of cancers, including hepatocellular carcinoma (HCC); however, it occasionally suppresses tumor progression. Moreover, it is virtually unknown how different sets of Notch ligands and receptors regulate the HCC development. In this study, we demonstrate that the expression of the Notch ligands, Delta-like 4 (Dll4) and Jagged-1 (Jag1), is upregulated during diethylnitrosamine-induced hepatocarcinogenesis. Dll4 is detected in the preneoplastic hepatocytes and HCC cells, but not in the normal hepatocytes, while Jag1 is expressed in the desmin-positive mesenchymal cells. Hepatocyte-specific Dll4 knockout abolishes the Notch1 signaling and suppresses the tumor progression. In contrast, Jag1 deletion induces the ectopic expression of Dll4 in hepatocytes along with the loss of Notch2 signaling, leading to the tumor progression. These results indicate that the two distinct Notch signals, Dll4/Notch1 and Jag1/Notch2, are antagonistic to each other, exerting opposite effects on HCC progression.

Numerical-Experimental Plastic-Damage Characterisation of Additively Manufactured 18Ni300 Maraging Steel by Means of Multiaxial Double-Notched Specimens

Additive manufacturing (AM) has become a viable option for producing structural parts with a high degree of geometrical complexity. Despite such trend, accurate material properties, under diversified testing conditions, are scarce or practically non-existent for the most recent additively manufactured (AMed) materials. Such data gap may compromise component performance design, through numerical simulation, especially enhanced by topological optimisation of AMed components. This study aimed at a comprehensive characterisation of laser powder bed fusion as-built 18Ni300 maraging steel and its systematic comparison to the conventional counterpart. Multiaxial double-notched specimens demonstrated a successful depiction of both plastic and damage behaviour under different stress states. Tensile specimens with distinct notch configurations were also used for high stress triaxiality range characterisation. This study demonstrates that the multiaxial double-notched specimens constitute a viable option towards the inverse plastic behaviour calibration of high-strength additively manufactured steels in distinct state of stress conditions. AMed maraging steel exhibited higher strength and lower ductility than the conventional material.

Description of three new species of biting midge of the genus emAtrichopogon/em Kieffer (Diptera: Ceratopogonidae) from Brazilian Amazon.

Three new species of Atrichopogon with spotted wings of the family Ceratopogonidae (Diptera: Culicomorpha) from the Amazonas State of Brazil are described and illustrated. Male and female specimens of Atrichopogon janseni sp. nov., A. riopardensis sp. nov., and A. sergioluzi sp. nov. were associated by wing pigmentation patterns: with two darker spots, one over r-m and the other one in cell r3, posterior to the apex of R3. Male A. janseni sp. nov. have tergite 9 that is 2.5 × as broad as long, not extending to the apex of gonocoxite, and sternite 9 stout, sub-trapezoidal, with distal margin with a row of stout spines; gonostylus of A. janseni sp. nov. is spatulate, with distinct middle notch. Atrichopogon riopardensis sp. nov. is separated from other species with similar wing patterns and forked gonostylus, with gonostylus forked near midlength, with longitudinal furrow, inner portion short and fingernail-like, outer portion elongate and apically curved directed mesally; outer portion is 4 × longer than inner, with one long seta in basal 1/3. Contrastingly, A. sergioluzi sp. nov. has a small fork at the apex of gonostylus, and cercus broad basally with rounded tip. Females of the three new species are very similar (as are other females in this group), and it is probably not possible to distinguish them using the morphological features described. With the addition of the three new species described here, there are now known 15 Neotropical species of Atrichopogon with pigmented wings.

Two new species of snailfishes of the genus emCareproctus/em (Cottoidei: Liparidae) from the western North Pacific Ocean with a range extension of emCareproctus brevipectoralis/em.

Three snailfish species of Careproctus (Cottoidei: Liparidae), including two new species, are described from the deep Pacific Ocean off northern Honshu Island, Japan. Careproctus cyanogladius sp. nov., collected from the depths of 2,698-3,223 m, can be distinguished from congeneric species by having the following combination of characters: vertebrae 63; dorsal-fin rays 57-58; anal-fin rays 50; principal caudal rays 7; pectoral-fin rays 29-31; pectoral fin without a notch; uppermost pectoral-fin base below a horizontal through posterior margin of maxillary; pelvic disk oval; chin pores well separated; teeth simple; pyloric caeca 6-10; body pale blue or gray when fresh. The other new species, Careproctus orri sp. nov., collected from depths of 640-808 m, can be distinguished from congeneric species by having the following combination of characters: vertebrae 58-60; dorsal-fin rays 52-53; anal-fin rays 47-49; pectoral-fin rays 34-35; pectoral fin with a distinct notch; cephalic pores 2-6-7-2, chin pores single, unpaired; gill slit extending ventrally to 5th or 6th pectoral-fin ray; teeth strongly trilobed; peritoneum dark, stomach white. In addition, one specimen of Careproctus brevipectoralis Chernova, Thiel Eidus, 2020 was newly collected from Japan, and is redescribed here in detail with comments on intraspecific variations.

Pulmonary Doppler Notch Pattern in Relation with Pulmonary Vascular Resistance Index in Patients with Atrial Septal Defect

Background: Atrial septal defect (ASD) potentially causes pulmonary hypertension (PH) and increased pulmonary vascular resistance (PVR). In previous studies, pulmonary Doppler notch was evident in patients with high PVR. There was a distinct notch pattern in different types of PH. This study aims to examine whether echocardiographic notch pattern could estimate PVR in secundum ASD patients.
 Methods: Cross sectional study was conducted in secundum ASD patients ≥18 years old who underwent clinically indicated right heart catheterization. Association of notch presence and PVR index (PVRi) and correlation of notch ratio (NR), a parameter of notch location, and PVRi were analyzed. The best cut off of NR value was obtained using the receiver operating characteristics curve.
 Results: Among 60 patients, the notch was present in 50 patients (83%) and significantly associated with PVRi ≥6 WU.m2 (p<0.0001). Notch ratio and PVRi had a moderate negative correlation (r=-0.410, p=0.003). Receiver operating characteristics curve with area under curve 0.709 showed that cutoff NR value of 1.635 was a good discriminator for PVRi ≥6 WU.m2, with 70% sensitivity, 70% specificity, 56.8% positive predictive value, and 14.9% negative predictive value. Patients were then classified into three groups of notch pattern based on its presence and location, which was significantly associated with PVRi (p<0.0001).
 Conclusions: Pulmonary Doppler notch pattern is associated with PVRi in secundum ASD patients. This may be used clinically to estimate PVR to aid patient selection for further invasive investigation.
 
 Keywords: atrial septal defect, Doppler echocardiography, notch, notch ratio, pulmonary vascular resistance

Three distinct mechanisms, Notch instructive, permissive, and independent, regulate the expression of two different pericardial genes to specify cardiac cell subtypes.

The development of a complex organ involves the specification and differentiation of diverse cell types constituting that organ. Two major cell subtypes, contractile cardial cells (CCs) and nephrocytic pericardial cells (PCs), comprise the Drosophila heart. Binding sites for Suppressor of Hairless [Su(H)], an integral transcription factor in the Notch signaling pathway, are enriched in the enhancers of PC-specific genes. Here we show three distinct mechanisms regulating the expression of two different PC-specific genes, Holes in muscle (Him), and Zn finger homeodomain 1 (zfh1). Him transcription is activated in PCs in a permissive manner by Notch signaling: in the absence of Notch signaling, Su(H) forms a repressor complex with co-repressors and binds to the Him enhancer, repressing its transcription; upon alleviation of this repression by Notch signaling, Him transcription is activated. In contrast, zfh1 is transcribed by a Notch-instructive mechanism in most PCs, where mere alleviation of repression by preventing the binding of Su(H)-co-repressor complex is not sufficient to activate transcription. Our results suggest that upon activation of Notch signaling, the Notch intracellular domain associates with Su(H) to form an activator complex that binds to the zfh1 enhancer, and that this activator complex is necessary for bringing about zfh1 transcription in these PCs. Finally, a third, Notch-independent mechanism activates zfh1 transcription in the remaining, even skipped-expressing, PCs. Collectively, our data show how the same feature, enrichment of Su(H) binding sites in PC-specific gene enhancers, is utilized by two very distinct mechanisms, one permissive, the other instructive, to contribute to the same overall goal: the specification and differentiation of a cardiac cell subtype by activation of the pericardial gene program. Furthermore, our results demonstrate that the zfh1 enhancer drives expression in two different domains using distinct Notch-instructive and Notch-independent mechanisms.

MORPHOLOGY AND MORPHOMETRY OF SCAPULAE IN TERTIARY CARE HOSPITAL IN K.M. MEDICAL COLLEGE AND HOSPITAL, MATHURA (UP).

Introduction: The shoulder joint is the most important joint in the body and limitation of this joint movements affects the quality of life. Severe shoulder injury and glenohumeral arthritis causes shoulder dysfunction with devasting results, and to restore normal functions orthopaedic surgeon can perform total shoulder arthroplasty, or reverse shoulder prosthesis, or hemiarthroplasty.Lateral angle of the scapula becomes truncated and broadened and bears the glenoid cavity which articulates with the humerus in the shoulder joint. Shoulder joint is formed when the glenoid cavity is connected with the head of the humerus. This study was carried out to study the morphology and morphometry of the glenoid cavity as dimensions of the glenoid cavity are important in designing and fitting of glenoid components for total shoulder arthroplasty.
 Material and Methods: A total of 100 unpaired dry scapula were isolated from the department of Anatomy. Two groups were made according to the side of the scapula and were equally divided into 50 right and 50 left scapula each. Bones were segregated and inspected individually.To classifying shape of the glenoid fossa, presence or absence of a glenoid notch was used and were classified into 3 types, pear shaped or inverted comma shape in presence of a glenoid notch & oval in absence of notch. The pear shaped shows indistinct glenoid notch and the inverted comma shaped shows a distinct notch.
 Results: On the basis of presence or absence of the glenoid notch. 56%, 28% & 16% glenoid cavities of right side were pear shaped, inverted comma shaped and oval shaped respectively. On the left side 60%, 30% & 10%were pear shaped, inverted comma shaped and oval shaped respectively. In total 58% glenoid cavities were pear shaped, 29% were inverted comma shaped and 13% were oval shaped.Average length and breadth of scapulae included in this study was 134.07±13.87 mm and 98.43 ±9.58 mm respectively.Mean glenoid cavity index of right side was 48.12 to 86.87 %, while in left side it was 44.21 – 84.78%.
 Conclusion: The most common glenoid cavity was pear shaped followed by inverted comma shaped and the oval glenoid cavity. Mean glenoid cavity index of right side was 48.12 to 86.87 %, while in left side it was 44.21 – 84.78%.