Three wastewater samples, agro wastewater, diary wastewater, distillery wastewater from nearby Qasimabad Hyderabad, were utilized as substrate in microbial fuel cell (MFC) to generate electricity. Along with electricity generation the MFCs can successfully helps in treating same samples. The parameters like pH, TS, TSS, TDS, BOD and COD were analyzed for all four samples. The COD removal efficiency of the MFC was analyzed using standard reflux method. MFC was efficient in COD removal, 55%, 70% and 99% COD removal was observed after 5, 10 and 20 days respectively of operation of MFC with municipal waste as a substrate. 50%, 75% and 99% COD removal was observed after 5, 10 and 20 days respectively of operation of MFC with dairy waste as a substrate, 40%, 60% and 99% COD removal was observed after 5, 10 and 20 days respectively of operation of MFC with agro waste as a substrate, 35%, 54% and 70% COD removal was observed after 5, 10 and 20 days respectively of operation of MFC with distillery waste as a substrate.