Potential of New Microbial Isolates for Biosurfactant Production using Combinations of Distillery Waste with other Industrial Wastes

Kirti V Dubey,Sanjeev Singh,Sudhir U Meshram,Pravin N Charde,Santosh K Yadav,Asha A Juwarkar

doi:10.4172/2157-7463.s1-002

Abstract

In the present study, combinations of Distillery Waste (DW) with other industrial wastes viz. curd Whey Waste (WW), Fruit Processing Waste (FPW) and Sugar Industry Effluent (SIE) were evaluated to replace the use of water that was reported earlier for biosurfactant production from 1: 3 diluted distillery waste by using four new bacterial cultures BS-A, BS-J, BS-K and BS-P, isolated from soil collected from a distillery unit. These isolates have the potential to produce biosurfactant from these individual wastes and in their combinations. Highest biomass and biosurfactant yields with higher reduction in the Chemical Oxygen Demand (COD), total sugars, nitrogen and phosphate levels were obtained in 1:1:1 proportion of DW+WW+FPW followed by DW+WW+ SIE and individual wastes. The combinations of wastes improved the yields of biosurfactants by 18-41% and reduced COD of the combined wastes by 76-84.2%. Total sugars, nitrogen and phosphate levels reduced in the range of 79-86%, 58-71% and 45-59%, respectively. Among the four microbial isolates tested, BS-J and BS-P were the efficient biosurfactant producers and were identified as Kocuria turfanesis and Pseudomonas aeruginosa based on the 16S rDNA sequence and phylogenetic analyses. Benefits derived by using combined distillery waste with other wastes are improved production of biosurfactant as resource and saving precious water and the costly nutrients with concomitant reduction in pollution load of the wastes.

Highlights

Biosurfactants are defined as a class of surface-active molecules synthesized by microorganisms
Present study was carried out with an aim to replace the use of precious water by some appropriate industrial waste, so that distillery waste can be used as no-cost medium for biosurfactant production after combination with industrial wastes instead of water
To evaluate a suitable combination of distillery waste with other industrial wastes for biosurfactant production, distillery waste and other different types of waste waters, viz. sugar industry effluent, curd whey, and fruit processing waste were collected from different industries during winter season in the month of December because of plenty availability of milk, variety of fruits and sugar cane which are utilized for industrial production of curd, jam and jellies and cane sugar, respectively (Table 1)

Summary

Introduction

Biosurfactants are defined as a class of surface-active molecules synthesized by microorganisms. Apart from being active at extreme temperatures, pH and salinity, biosurfactants can be produced from industrial wastes and from byproducts [7]. This last feature makes cheap production of biosurfactants possible as it allows utilization of waste substrates so that application of biosurfactants in environmental remediation can be realized and environmental use is currently considered to be one of the larger markets for biosurfactants [6,8,9]. Economical large scale production for established and new applications of biosurfactants remains a challenge [10]. The main factor limiting commercialization of biosurfactants is associated with non-economical large-scale production. Important prerequisites for the competitive production of biosurfactants include high biosurfactant yields, alternative low cost substrates, and cost-effective bioprocesses

Methods

Results

Conclusion