Distant Relatives Research Articles

Embedding-based alignment: combining protein language models with dynamic programming alignment to detect structural similarities in the twilight-zone.

Language models are routinely used for text classification and generative tasks. Recently, the same architectures were applied to protein sequences, unlocking powerful new approaches in the bioinformatics field. Protein language models (pLMs) generate high-dimensional embeddings on a per-residue level and encode a "semantic meaning" of each individual amino acid in the context of the full protein sequence. These representations have been used as a starting point for downstream learning tasks and, more recently, for identifying distant homologous relationships between proteins. In this work, we introduce a new method that generates embedding-based protein sequence alignments (EBA) and show how these capture structural similarities even in the twilight zone, outperforming both classical methods as well as other approaches based on pLMs. The method shows excellent accuracy despite the absence of training and parameter optimization. We demonstrate that the combination of pLMs with alignment methods is a valuable approach for the detection of relationships between proteins in the twilight-zone. The code to run EBA and reproduce the analysis described in this article is available at: https://git.scicore.unibas.ch/schwede/EBA and https://git.scicore.unibas.ch/schwede/eba_benchmark.

CONFIRMATION OF INHERITANCE ACQUISITION IN THE PRACTICE OF POLISH COURTS – SELECTED ISSUES

Amendment to the Civil Code rules on succession, introduced on 2 April 2009, extended the range of statutory heirs by more distant relatives than lineal parents and collateral siblings’ descendants, by including stepchildren within the range of statutory heirs. This article is an attempt of the author – academic lecturer and, at the same time, practising advocate – to reflect on the practical consequences of that legislative reform. In particular, the problem arose as to how the extension of the range of statutory heirs influences the efficiency and duration of court proceedings in matters for the confirmation of inheritance acquisition. The article presents the most crucial procedural problems, often following from inconsistent judicial practice, which lead to the protraction of court proceedings, both when we have to do with intestate and testamentary succession. Another question depicted is the causes of delivering two or more decisions confirming the acquisition of inheritance from the same deceased person and methods of eliminating such defective rulings from legal practice. The presented problems should provide a stimulus for necessary legislative amendments in this area.

Genetic profiling and PVY resistance identification of potato germplasm resources.

Excellent germplasm resources are the foundation for cultivating high-quality, disease-resistant, and stress-tolerant varieties. In this study, simple sequence repeat (SSR) markers were used to identify 138 potato accessions collected from worldwide, and genetic cluster analysis was used to characterize the genetic diversity of the tested germplasm resources. The Potato virus Y (PVY) resistance of these potato accessions was identified by artificial friction inoculation combined with molecular marker detection, and potato accessions with different PVY resistance were screened based on disease index and incidence rate. Using SSR markers, 138 potato accessions were identified, and the results showed that the genetic distances between the tested potato germplasm resources ranged from 0.025 to 0.660, and the genetic similarity coefficients ranged from 0.489 to 0.975. The 138 accessions could be clustered into five subgroups using Unweighted Pair-Group Method with Arithmetic Mean (UPGMA). Among them, Z173, Biyin No. 4, Suyin No. 2, XN995, XN987, Biyin No 22, Bibiao104, Sarpo mira, XN996, XN979, Desiree, RUNSHI, Actrice, Jia 1219, Heyin No 12, and Moyin No.1 have relatively distant genetic relationship with another 122 accessions. Based on the disease index, the following different accessions were screened: five highly resistant, 11 resistant, 45 moderately resistant, 35 susceptible, and 42 highly susceptible. Fourteen resource materials with good resistance (disease index ≤ 33.74%, and a grading of high resistance (HR) or medium resistance (MR); incidence rate ≤ 67.58%) were identified. By combining genetic cluster analysis and PVY resistance identification, six accessions showed PVY resistance and had distant genetic relationships with other accessions selected which provided important materials for disease resistance breeding and quality improvement of potato. In this study, the genetic diversity and PVY resistance of global potato germplasm resources was explored, and potato germplasm materials with important utilization value were screened. The results obtained in this study could provide important references for the research and utilization of global potato germplasm resources.

Analysis of Germin-like protein genes family in Vitis vinifera (VvGLPs) using various in silico approaches.

Germin-like proteins (GLPs) play an important role against various stresses. Vitis vinifera L. genome contains 7 GLPs; many of them are functionally unexplored. However, the computational analysis may provide important new insight into their function. Currently, physicochemical properties, subcellular localization, domain architectures, 3D structures, N-glycosylation & phosphorylation sites, and phylogeney of the VvGLPs were investigated using the latest computational tools. Their functions were predicted using the Search tool for the retrieval of interacting genes/proteins (STRING) and Blast2Go servers. Most of the VvGLPs were extracellular (43%) in nature but also showed periplasmic (29%), plasma membrane (14%), and mitochondrial- or chloroplast-specific (14%) expression. The functional analysis predicted unique enzymatic activities for these proteins including terpene synthase, isoprenoid synthase, lipoxygenase, phosphate permease, receptor kinase, and hydrolases generally mediated by Mn+ cation. VvGLPs showed similarity in the overall structure, shape, and position of the cupin domain. Functionally, VvGLPs control and regulate the production of secondary metabolites to cope with various stresses. Phylogenetically VvGLP1, -3, -4, -5, and VvGLP7 showed greater similarity due to duplication while VvGLP2 and VvGLP6 revealed a distant relationship. Promoter analysis revealed the presence of diverse cis-regulatory elements among which CAAT box, MYB, MYC, unnamed-4 were common to all of them. The analysis will help to utilize VvGLPs and their promoters in future food programs by developing resistant cultivars against various biotic (Erysiphe necator and in Powdery Mildew etc.) and abiotic (Salt, drought, heat, dehydration, etc.) stresses.

Identifying distant relatives using benchtop-scale sequencing

The genetic component of forensic genetic genealogy (FGG) is an estimate of kinship, often conducted at genome scales between a great number of individuals. The promise of FGG is substantial: in concert with genealogical records and other nongenetic information, it can indirectly identify a person of interest. A downside of FGG is cost, as it is currently expensive and requires chemistries uncommon to forensic genetic laboratories (microarrays and high throughput sequencing). The more common benchtop sequencers can be coupled with a targeted PCR assay to conduct FGG, though such approaches have limited resolution for kinship. This study evaluates low-pass sequencing, an alternative strategy that is accessible to benchtop sequencers and can produce resolutions comparable to high-pass sequencing. Samples from a three-generation pedigree were augmented to include up to 7th degree relatives (using whole genome pedigree simulations) and the ability to recover the true kinship coefficient was assessed using algorithms qualitatively similar to those found in GEDmatch. We show that up to 7th degree relatives can be reliably inferred from 1 × whole genome sequencing obtainable from desktop sequencers.

Interaksi Sosial Akun Twitter PT.Angkasa Pura II (Studi Analisis Isi)

Social interaction is a reciprocal relationship between individuals that mutually influences each other. Social interaction not only occurs in the real world but can also take place on social media accounts. Social media interactions happen when users engage with each other through available platforms. Social media provides convenience for users to interact with friends, family, and even distant relatives through the provided social media accounts without the need for face- to-face meetings. Our research aims to analyze the content posted by the Twitter account of PT. Angkasa Pura 2. This includes the types of posts, discussed topics, and how the content relates to the organization's communication goals. The research method used is descriptive research. Descriptive research employs an approach that is used to depict and define the characteristics of a phenomenon without making changes to it. This study will uncover and understand the root issues of "lack of interaction on the Twitter account of PT. Angkasa Pura 2". After this research, it will be possible to identify any conflicts that have occurred. Subsequently, the issues will be presented, and the best solutions for the progress of the PT. Angkasa Pura 2 Twitter account will be proposed.

CHEMICAL PROCESSING OF AGRICULTURE WASTES INTO VANILLIN, PULP AND GLUCOSE

Agrotechnical wastes from processing herbaceous plants consist of lignin and polysaccharides, which can be processed into monomers – phenols and carbohydrates. The prospects of chemical processing of several agrotechnical waste types with a high lignin content (flax shives, sunflower seed husks and buckwheat husks) into vanillin, pulp, and glucose by oxidation and acid hydrolysis were studied. It has been shown that despite the distant phylogenetic relationship of the studied plants, their lignins have a similar structure: they contain a similar amount of methoxyl groups (10–13 wt.% per lignin) and give close yields of aromatic aldehydes upon oxidation with nitrobenzene (17–19%) and oxygen (14–16%). In general, the suitability of agrotechnical wastes for oxidation to aromatic aldehydes determines by the lignin content. Among the studied wastes, flax shives are the most promising feedstock for chemical processing into vanillin and glucose. Cellulose-containing solid residues of oxidation process are more easily hydrolyzed compared to the initial lignocellulosic biomass. The inversion of glucose oligomers during the hydrolysis of cellulose with H2SO4 is limited by the hydrolysis of the tetra- and octamers.

Biological Characteristics of Feline Calicivirus Epidemic Strains in China and Screening of Broad-Spectrum Protective Vaccine Strains.

Feline calicivirus (FCV) is one of the most important pathogens causing upper respiratory tract diseases in cats, posing a serious health threat to these animals. At present, FCV is mainly prevented through vaccination, but the protective efficacy of vaccines in China is limited. In this study, based on the differences in capsid proteins of isolates from different regions in China, as reported in our previous studies, seven representative FCV epidemic strains were selected and tested for their viral titers, virulence, immunogenicity, and extensive cross-protection. Subsequently, vaccine strains were selected to prepare inactivated vaccines. The whole-genome sequencing and analysis results showed that these seven representative FCV strains and 144 reference strains fell into five groups (A, B, C, D, and E). The strains isolated in China mainly fall into groups C and D, exhibiting regional characteristics. These Chinese isolates had a distant evolutionary relationship and low homology with the current FCV-255 vaccine strain. The screened FCV-HB7 and FCV-HB10 strains displayed desirable in vitro culture characteristics, with the highest virus proliferation titers (109.5 TCID50/mL) at 36 h post inoculation at a dose of 0.01 MOI. All five cats infected intranasally with FCV-HB7 or FCV-HB10 strains showed obvious clinical symptoms of FCV. The symptoms of cats infected with the FCV-HB7 strain were more severe than those infected with the FCV-HB10 strain. Both the single-strain inactivated immunization and combined bivalent inactivated vaccine immunization of FCV-HB7 and FCV-HB10 induced high neutralizing antibody titers in five cats immunized. Moreover, bivalent inactivated vaccine immunization protected cats from FCV-HB7 and FCV-HB10 strains. The cross-neutralizing antibody titer against seven representative FCV epidemic strains achieved by combined bivalent inactivated vaccine immunization was higher than that achieved by single-strain immunization, which was much higher than that achieved by commercial vaccine FCV-255 strain immunization. The above results suggest that the FCV-HB7 and FCV-HB10 strains screened in this study have great potential to become vaccine strains with broad-spectrum protective efficacy. However, their immune protective efficacy needs to be further verified by multiple methods before clinical application.

Wide hybridization in Rapeseed-Mustard

The Brassicas are an important group of crops in India yielding oils and many vegetables. For improving cultivated brassicas, the distant relatives are of considerable value. Wide hybridization is the way which could combine the valuable features of parental species into the hybrid. Crosses among Brassicaspecies viz., B. juncea (AABB, 2n = 36), B. Carinata (BBCC, 2n = 34), B. napus (AACC, 2n = 38), B. campestris (AA, 2n = 20), wild relative of cultivated species B. fruticulosa (2n = 4x=32, FFFF), and B. tournefortii (2n=20, TT) were attempted with the objective to find out crossability among them, analyse chromosome association in inter-specific hybrids, generate genetic variability and combine desirable characters in a targeted genotypes/species.

Explaining mobilization for revolts by private interests and kinship relations.

Mobilization for revolts poses a significant challenge for rational choice theory because revolts are vulnerable to free-riding, which disincentivizes rational actors from mobilizing. Strong, informal relations such as kinship ties have been identified as factors that can shift the rational calculations of individuals and lead to mobilization for revolts. In social networks that are polarized by the presence of mobilized individuals, such as rebels, and actors opposing the mobilization effort such as the elite, kinship relations have not only a bridging effect but also a diverging one. Building on Tullock's private interest theory, we develop a framework in which kinship relations determine the extent of individual's payoffs and costs of mobilization for revolts against an elite. We posit that distant kin of the elite expect high payoffs of mobilization for revolts and face the lowest costs of mobilization for revolts by virtue of their position in the network of kinship relations. Using a unique, hand-collected dataset that reconstructs a revolt in Basel, Switzerland, in 1691, we test our framework and contribute to a better relational understanding of the mechanisms that lead rational actors to mobilize for revolts. Our analyses show that mobilization for revolts is mainly driven by distant kinship relations to the ruling elite rather than close kinship relations to the rebels.

Molecular detection of bornavirus in parrots imported to China in 2022

BackgroundAvian bornavirus (ABV) is a neurotropic virus, it has been established as the primary causative agent of proventricular dilatation disease (PDD). However, substantial international trade and transnational trafficking of wild birds occur, potentially enabling these birds to harbor and transmit pathogens to domestic poultry, adversely affecting their well-being. Real-time RT-PCR was employed to detect the presence of PaBV-4 in parrots imported to China in 2022.ResultsIn 2022, a total of 47 cloacal swabs from 9 distinct species of parrots were collected at the Wildlife Rescue Monitoring Center in Guangdong, China. The purpose of this collection was to detect the presence of PaBV-4. Using real-time PCR techniques, it was determined that the positive rate of PaBV-4 was 2.12% (1 out of 47) in parrots. The PaBV-4 virus was detected in a Amazona aestiva that had been adopted for one month. Conversely, all other species tested negative for the virus. Subsequently, the whole genome of the PaBV-4 GD2207 strains was sequenced, and the homology and genetic evolution between these strains and previously published PaBV-4 strains on GenBank were analyzed using DNAStar and MEGA7.0 software. The findings revealed that the full-length genome of PaBV-4 consisted of 8915 nucleotides and encoded six proteins. Additionally, it exhibited the highest nucleotide similarity (99.9%) to the GZ2019 strain, which causes death and severe clinical symptoms in Aratinga solstitialis. Furthermore, when compared to other strains of PaBV-4, the GD2207 strain demonstrated the highest amino acid homology with GZ2019. The phylogenetic analysis demonstrated that the GD2207 strain clustered with various strains found in Japanese, American, and German parrots, indicating a close genetic relationship with PaBV-4, but it revealed a distant relationship with PaBV-5 Cockg5 from America. Notably, the GD2207 was closely associated with the GZ2019 strain from Aratinga solstitialis in China.ConclusionThis study presents the preliminary identification of PaBV-4 in Amazona aestiva parrots, emphasizing its importance as the predominant viral genotype linked to parrot infections resulting from trade into China. Through genetic evolution analysis, it was determined that the GD2207 strain of PaBV-4 exhibits the closest genetic relationship with GZ 2019 (Aratinga solstitialis, China), M14 (Ara macao, USA), AG5 (Psittacus erithacus, USA) and 6758 (Ara ararauna, Germany) suggesting a shared ancestry.

Distant Supervision-based Relation Extraction for Literature-Related Biomedical Knowledge Graph Construction

Background: The task of relation extraction is a crucial component in the construction of a knowledge graph. However, it often necessitates a significant amount of manual annotation, which can be time-consuming and expensive. Distant supervision, as a technique, seeks to mitigate this challenge by generating a large volume of pseudo-training data at a minimal cost, achieved by mapping triple facts onto the raw text. Objective: The aim of this study is to explore the novelty and potential of the distant supervisionbased relation extraction approach. By leveraging this innovative method, we aim to enhance knowledge reliability and facilitate new knowledge discovery, establishing associations between knowledge from specific biomedical data or existing knowledge graphs and literature. Methods: This study presents a methodology to construct a biomedical knowledge graph employing distant supervision techniques. Through establishing links between knowledge entities and relevant literature sources, we methodically extract and integrate information, thereby expanding and enriching the knowledge graph. This study identified five types of biomedical entities (e.g., diseases, symptoms and genes) and four kinds of relationships. These were linked to PubMed literature and divided into training and testing datasets. To mitigate data noise, the training set underwent preprocessing, while the testing set was manually curated. Results: In our research, we successfully associated 230,698 triples from the existing knowledge graph with relevant literature. Furthermore, we identified additional 205,148 new triples directly sourced from these studies. Conclusion: Our study markedly advances the field of biomedical knowledge graph enrichment, particularly in the context of Traditional Chinese Medicine (TCM). By validating a substantial number of triples through literature associations and uncovering over 200,000 new triples, we have made a significant stride in promoting the development of evidence-based medicine in TCM. The results underscore the potential of using a distant supervision-based relation extraction approach to both validate and expand knowledge bases, contributing to the broader progression of evidence-based practices in the realm of TCM.

Familial relatedness in genetic frontotemporal dementia cohorts: findings from the international Frontotemporal Dementia Prevention Initiative

AbstractBackgroundGiven the rarity of genetic frontotemporal dementia (FTD), researchers across the world have come together to form the FTD Prevention Initiative (FPI) in an effort to improve prevention trials design. As this initiative begins to bring together large‐scale data from worldwide cohort series, including ALLFTD in North America and GENFI in Europe and Canada, it is critical for FPI to quantify the level of relatedness between all participants. Here we provide the most recent update to these ongoing analyses.MethodsGenome‐wide SNP genotyping data from 1,684 ALLFTD and 568 GENFI participants was used to perform lineage analyses using PLINK. Briefly, QC was performed similarly in all datasets to remove individuals with low call rate and filter autosomal SNPs for missingness, frequency, and deviation from Hardy‐Weinberg equilibrium. Genetic ancestry was inferred by projecting genotyped samples into the principal components of the 1000 Genomes reference panel, using R package bigsnpr. Overlapping ALLFTD and GENFI genotyping data was then used, in a two‐stage approach, to calculate pairwise identity‐by‐descent (IBD) estimates and KING coefficients, followed by family‐network identification and pedigree reconstruction using PRIMUS.ResultsFirst, we calculated IBD estimates among all participants by restricting pairs to those with estimates>0.1875 (up to second‐degree relatives). Overall, we identified a total of 292 second‐degree family networks, including 168 ALLFTD and 120 GENFI families, mostly associated with pathogenic variants in the 3 major FTD‐causing genes. We also identified 4 family networks with participants enrolled in both the ALLFTD and GENFI series, as well as several multi‐site families within the ALLFTD consortium. This first, overall approach allowed us to predict close relationships even between individuals with different ancestral backgrounds, including at least 4 confirmed admixed families. More distant relationships were also detected within ALLFTD and GENFI by performing ancestry‐based analysis among participants with estimated European ancestry, using the KING‐robust algorithm.ConclusionsThese lineage analyses allowed us to identify, otherwise unknown, close (and distant) relatives from different study sites, as well as within the ALLFTD and GENFI series. This dataset will be a crucial resource to increase statistical accuracy and power in upcoming collaborative FPI studies.

Bridging the past and the future: the ECtHR application of the prohibition of discrimination to distant private legal relationships

Bridging the past and the future: the ECtHR application of the prohibition of discrimination to distant private legal relationships

Factors affecting social phobia among Chinese college students in the context of COVID-19 pandemic: a cross-sectional study.

Social phobia (SP) refers to excessive anxiety about social interactions. College students, with their exposure to academic, familial, and job-related pressures, are an ideal population for early screening and intervention of social phobia. Additionally, COVID-19 prevention measures including keeping social distance may further impact social phobia. This study aims to investigate the influencing factors of social phobia among Chinese college students and to tentatively explore the impact of COVID-19 prevention measures on social phobia. Respondents were recruited through Chinese Internet social platforms for an online survey. College students' social phobia scores in pre- and early-COVID-19 periods were measured using Peters' short form of the Social Interaction Anxiety Scale and Social Phobia Scale (SIAS-6/SPS-6). Demographic information, family information, social relations, self-evaluation, and subjective feelings regarding the impact of COVID-19 preventive measures on social phobia were collected. A multivariable logistic regression model was used to analyze the influencing factors. A total of 1859 valid questionnaires were collected, revealing that the social phobia scores increased from 12.3 ± 11.9 to 13.4 ± 11.9 between pre- and early-COVID-19 periods, with an increase of 1.0 ± 6.4 (p < 0.001). Low GPA rank, mobile phone dependence, distant family relationships, indulgent parents, childhood adversity, and childhood bullying were risk factors for social phobia among Chinese college students. Female gender, being a senior university student or postgraduate, satisfaction with physical appearance, self-reported good mental health and high level of interpersonal trust were protective factors for social phobia. Although most respondents believed that COVID-19 prevention measures (e.g., mask wearing and social distancing rules) reduced their social phobia, these measures were not significantly associated with social phobia levels in the multivariable analyses. In conclusion, Chinese college students' social phobia was widely influenced by diverse factors and warrants increased attention, with early intervention aimed at high-risk individuals being crucial for their mental health. Additional research is necessary to understand the impact of COVID-19 preventive measures on social phobia among college students.

Determining lineages between individuals with high-density mitochondrial and Y-chromosomal single-nucleotide polymorphisms.

Genetic genealogy has been more frequently used in forensic investigations in identifying criminals. However, the current genetic genealogy applications usually do not consider lineage markers (including both Y and mitochondrial deoxyribonucleic acid (DNA)), which is probably because not all distant relatives share the same lineage markers. In addition, there is no study to show how to use lineage markers and what methods or thresholds should be applied in genetic genealogy. In this study, we developed a method to quickly determine if two single-nucleotide polymorphism (SNP) profiles are from the same paternal or material lineages by using a mismatch frequency of the SNPs in Y-chromosomal or mitochondrial DNA. For both Y and mitochondrial SNPs, profile pairs from the same or different lineages can be decided with high accuracies (i.e., 0.380% or 0.157% error rates with Y and mitochondrial DNA, respectively). With kinship coefficient filtering based on autosomal SNPs, the accuracies of determining maternal and paternal lineage can be further improved (i.e., 0.120% or 0.057% error rates with Y and mitochondrial DNA, respectively, using a threshold of kinship coefficient >0). This study shows that lineage markers can be very powerful tools with high accuracies to determine lineages, which could help solve cases and reduce costs for genetic genealogy investigations.

Draft genomes of novel avian Chlamydia abortus strains from Australian Torresian crows (Corvus orru) shed light on possible reservoir hosts and evolutionary pathways.

Chlamydia abortus, an obligate intracellular bacterium, is a major causative agent of reproductive loss in ruminants, with zoonotic potential. Though this pathogen is primarily known to infect livestock, recent studies have detected and isolated genetically distinct avian strains of C. abortus from wild birds globally. Before this study, only five avian C. abortus genomes were publicly available. Therefore, we performed culture-independent probe-based whole-genome sequencing on clinical swabs positive for avian C. abortus obtained from Australian Torresian crows (Corvus orru) in 2019 and 2020. We successfully obtained draft genomes for three avian C. abortus strains (C1, C2 and C3), each comprising draft chromosomes with lengths of 1 115 667, 1 120 231 and 1 082 115 bp, and associated 7 553 bp plasmids, with a genome completeness exceeding 92 %. Molecular characterization revealed that these three strains comprise a novel sequence type (ST333), whilst phylogenetic analyses placed all three strains in a cluster with other avian C. abortus genomes. Interestingly, these three strains share a distant genomic relation (2693 single nucleotide variants) with the reference strain 15-58d/44 (ST152), isolated from a Eurasian magpie (Pica pica) in Poland, highlighting the need for more publicly available genomes. Broad comparative analyses with other avian C. abortus genomes revealed that the three draft genomes contain conserved Chlamydia genomic features, including genes coding for type III secretion system and polymorphic membrane proteins, and potential virulence factors such as the large chlamydial cytotoxin, warranting further studies. This research provides the first avian C. abortus draft genomes from Australian birds, highlighting Torresian crows as novel reservoir hosts for these potential pathogens, and demonstrates a practical methodology for sequencing novel Chlamydia genomes without relying on traditional cell culture.

Rights and Position of Home Children In The Sanahu Community of West Part of Seram District

Introduction: The offspring of a husband and wife being taken as children for another family, whether close relatives or distant relatives based on an agreement which of course gives rise to the rights and position of the adopted child, for this reason it is necessary to establish the rights and position of the child. so it won't cause problems in the future.Purposes of the Research: To find out and analyze the rights and position of returning children in the Sanahu Community, West Seram Regency.Methods of the Research: This research was conducted using an empirical juridical approach which is a descriptive qualitative analysis study. The research attempts to describe the rights and position of returning children in the Sanahu Community, West Seram Regency. The workings of the empirical juridical or sociological juridical method in this research proposal, namely from the results of collecting and discovering data and information through literature study of the basic assumptions or presumptions used in answering the problems in this research, then inductive-verification testing is carried out on the latest facts existing in society.Results of the Research: The results of the research show that the rights and position of children returning home in the Sanahu Community of West Seram Regency are regulated based on customary rules that developed in Sanahu Village where adopted children will be classified as legitimate children of the parents who adopted them and have the position of biological children so that they have the right to the inheritance of that person. The parents adopted him, but the assets he inherited were only the joint assets of his adoptive parents. The child's inherited assets did not have the right to inherit them.

Detection of Feline Cupripoxvirus and Variation Analysis of Its VP1 Gene in Chengdu from 2020 to 2023

Feline calicivirus (FCV) is an important pathogen causing infectious diseases in the oral cavity and upper respiratory tract of cats. Research on its prevalence and genetic variations contributes to the prevention and treatment of related diseases, as well as the development of new vaccines. In this study, a total of 113 samples of eye, nose, and throat secretions were collected from cats with respiratory and oral inflammation symptoms in Chengdu area from 2020 to 2023. The samples were tested using the RT-PCR method. The VP1 gene region was then cloned, sequenced, and subjected to homology analysis and phylogenetic analysis with domestic and foreign FCV reference strains. The results showed that among the 113 samples, 24 tested positive for FCV, with a positive rate of 21.24%. The nucleotide homology between the 5 FCV VP1 genes obtained in this study and the reference strains ranged from 72.6% to 84.1%, while the amino acid homology ranged from 84.0% to 95.9%. Phylogenetic analysis showed that the strains obtained in this study clustered into two gene groups (GⅠ and GⅡ) in the domestic and foreign FCV strain evolution tree. Strains SMU-M2, SMU-M7, SMU-M13, and SMU-M71 belonged to the GⅡ subtype, while strain SMU-M8 belonged to the (GⅠ subtype. The FCV vaccine strain had a distant genetic relationship with the prevalent FCV strains in Chengdu. The study revealed that FCV is prevalent in the cat population of Chengdu, and there is a low genetic homology and significant sequence differences between the prevalent strains and vaccine strains. This suggests the need to evaluate the actual protective efficiency of existing commercial FCV vaccines and promote the development of new vaccines with better cross-protection against prevalent strains.

Teachers' Structuring of Culturally Responsive Social Relations and Secondary Students’ Experience of Warm Demand

Students who experience teacher warm demand (i.e., caring and high expectations) are typically more engaged and successful at school. Yet, relative to White students, students of color tend to report more distant relationships with their White teachers. Leveraging data from 179 6th–9th grade Measures of Effective Teaching project classroom videos, we tested whether teachers’ facilitation of culturally responsive social relations was associated with higher warm demand, and whether these social relations moderated associations between race and warm demand. Results showed that teachers’ promotion of culturally responsive social relations was associated with warm demand, and that this was magnified for White teachers in relation to their discipline practices. Findings suggest that taking a culturally responsive approach to facilitating classroom social relations is critical for teachers seeking to improve students’ experiences of warm demand.