Distant Relatives Research Articles

SICILIAN-NORMAN TRAIL IN THE ORIGIN OF THE MOSCOW BOYARS OF THE VORONTSOV AND KHVOSTOV DYNASTIES

The article contains the results of a study of the family relationship of the famous Moscow boyars Vorontsovs and Khvostovs, who served in the Moscow principality as thousands and governors for a hundred years, starting from the second half of the 13th century, as well as the degree of their relationship with the Moscow princes. The work uses a comprehensive approach to the analysis of various archival sources in combination with the genetic data of the y-chromosome of modern descendants. The documentary base of the work was made up of European and Russian chronicle sources of the 12th-16th centuries, archival information from the regional archives of Russia, European genealogical tables on the history of European states and genealogies of Russian princes and nobles. The search showed that in the middle of the XII century the ancestors of the Vorontsovs and Bassovol-Khvostovs could be siblings from the family of the knight Count Loritello Robert I Bassavil, a South Italian Norman from the Rollonid family. Their distant relationship with the princes from the Rurikovich-Monomashich dynasty was established. At the same time, the legend of the appearance of the Resh princes Vorontsov from the marriage unions of the descendants of the Holy Roman Emperor Frederick Barbarossa and Count Robert I Bassavil is substantiated. The genetic affiliation of the Vorontsov and Bassovol-Khvostov clans to the grand ducal family confirms the version of the murder of the Moscow thousandth boyar Alexei Khvost in 1357 as a possible contender for the throne and may be one of the main reasons for the cruel reprisal of Ivan the Terrible against members of this family close to the court long before the oprichnina and the expulsion of their relatives to Kazan immediately after its conquest.

STRATEGI BERTAHAN HIDUP MASYARAKAT NELAYAN MELALUI JARINGAN SOSIAL DI DESA CIKOANG, KABUPATEN TAKALAR

AbstractIndonesia consists of tens of thousands of islands and is known as a maritime country that has a very wide sea territory and enormous marine wealth. However, the living conditions of fishermen are generally relatively poor. In such conditions, social networks are very helpful as a strategy for fishermen to survive. This study aims to look at social networks in fishing communities in Cikoang Village, Takalar Regency. The method used is the mix method, namely a combination of methods by combining qualitative and quantitative analysis. This study found that the social network of fishing communities is divided into internal and external social networks. The internal social network of fellow fishermen, close family and neighbors is the social capital that is often used by the fishing community of Cikoang village in a survival strategy. Some fishermen use external social networks in survival strategies such as assistance from distant relatives who are migrating. Fishermen's external social network is not as strong as fishermen's internal social network. Fishermen who have families who are also neighbors are the most strategic social network for survival in the Cikoang Fishermen's Community, Takalar.

Post-Fire Recovery of Plant Biodiversity Changes Depending on Time Intervals since Last Fire in Semiarid Shrublands

Fire is a key disturbance affecting plant biodiversity patterns and evolution. Although a wide range of studies have shown important impacts of fire on vegetation, most have focused on taxonomic diversity, with less emphasis on other aspects of biodiversity, such as functional and phylogenetic diversity. Therefore, we assessed the recovery of biodiversity facets across different times since the last fire in semiarid shrublands in Northeast Iran. We quantified changes in plant biodiversity facets, including taxonomic, functional, and phylogenetic diversity, and the diversity of seven functional traits in five ecologically comparable sites that have experienced wildfire disturbances at short-term (1 and 4 year sites) and long-term (10 and 20 year sites) intervals, in com- parison to an unburnt site. Our results showed significant changes in all biodiversity facets related to the year since the last fire, with a significant increase in biodiversity and diversity of functional traits under long-term rather than short-term conditions, and in comparison to the unburned site. We conclude that wildfire influences the presence of plant species with distant functional and evolutionary relatedness and causes an increase in plant species and diversity of functional traits de- pending on time intervals. Therefore, wildfire can promote positive effects on the recovery of bio- diversity aspects and the evolution of vegetation in semiarid shrublands.

Developmental Validation of the Illumina Infinium Assay Using the Global Screening Array on the iScan System for Use in Forensic Laboratories

Microarray processing, which interrogates hundreds of thousands of single nucleotide polymorphisms (SNPs) across the human genome, has recently gained traction in forensics due to its use in forensic genetic genealogy, which is based on analysis using SNPs to compare distant relatives in publicly curated databases for the purposes of developing investigative leads or identifying human remains. To date, there has been no published developmental validation of microarray processing using the Scientific Working Group on DNA Analysis Methods (SWGDAM) Validation Guidelines for DNA Analysis Methods and Federal Bureau of Investigation Quality Assurance Standards. Validation of these methods are warranted to identify samples suitable for microarray analysis and to assess the quality of the data obtained before upload to genealogical databases. In this study, we validated the Global Screening Array for use in forensic investigations according to SWGDAM guidelines, including the following studies: precision and accuracy, sensitivity, contamination, degradation, species specificity, mock case-type samples, mixtures, repeatability and reproducibility, and stability. Results indicated accurate genotype calls with SNP call rates >95% at DNA input as low as 0.20 ng. In addition to SNP call rate, we developed interpretation thresholds for signal intensity and heterozygosity to allow for sample quality assessment and identification of highly degraded and/or non-human DNA. This study demonstrates that high-quality data can be generated from multiple sample types, including mock forensic evidence that simulated the challenges that are often encountered in forensic cold cases.

Pollen-stigma incompatibility within and between species: Tread lightly, sedate the dogs, and don't wake the guards!

In a recent issue of Nature, Huang etal. identify and show how to overcome the barriers to successful pollen germination after interspecific crosses.1 Their findings answer a long-standing question about reproductive barriers in flowering plants and open the door to harnessing genetic diversity of distant relatives for crop improvement.

Comparative analysis of the complete plastomes of nine Pimpinella species (Apiaceae) from China.

Pimpinella L. is one of the large genera in the Apiaceae family. In a previous study, the molecular phylogenies of Pimpinella were explored using nuclear ribosomal DNA internal transcribed spacers (ITS) and several chloroplast DNA segments. There have been few studies conducted on chloroplast genomes in Pimpinella, which has limited systematic understanding of this genus. We assembled the complete chloroplast genomes of nine Pimpinella species from China using data generated from next generation sequencing (NGS). The chloroplast (cp) DNA used were standard double-stranded molecules, ranging from 146,432 base pairs (bp) (P. valleculosa) to 165,666 bp (P. purpurea) in length. The circular DNA contained a large single-copy (LSC) region, small single-copy (SSC) region, and pair of inverted repeats (IRs). The cp DNA of the nine species contained 82-93 protein-coding genes, 36-37 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes, respectively. Four species (P. smithii, P. valleculosa, P. rhomboidea, and P. purpurea) exhibited striking distinctions in genome size, gene number, IR boundary, and sequence identity. We confirmed the non-monophyly of the Pimpinella species on the basis of the nine newly identified plastomes. The distant relationship between the above-mentioned four Pimpinella species and Pimpinelleae was indicated with high support values. Our study provides a foundation for future in-depth phylogenetic and taxonomic studies of genus Pimpinella.

Salt-Tolerant Crops: Time to Deliver.

Despite the numerous advances made in our understanding of the physiology and molecular genetics of salinity tolerance, there have been relatively few applications of these to improve the salt tolerance of crops. The most significant advances have historically utilized intraspecific variation, introgression of traits from close crop wild relatives, or, less frequently, introgression from more distant relatives. Advanced lines often fail due to difficulties in the introgression or tracking of traits or due to yield penalties associated with the alleles in nonsaline environments. However, the greatest limitation is that salinity is not a primary trait for breeders. We must close the gap between research and delivery, especially for farmers who have precious few alternatives. These efforts should include a reassessment of old techniques such as grafting current crops with salt-tolerant hybrid rootstocks. Alternatively, future crops can be produced via domestication of salt-tolerant wild species-an approach that is now feasible in our lifetime. Expected final online publication date for the Annual Review of Plant Biology, Volume 74 is May 2023. Please see http://www.annualreviews.org/page/journal/pubdates for revised estimates.

Türk böbrek donörlerinin demografik özellikleri ve alıcı ile verici akrabalık ilişkisinin postoperatif parametrelere etkisi: Tek merkez deneyimi

Objective: Kidney transplantation (KTx) is the most effective treatment option for patients with end-stage renal disease (ESRD). Live donor kidney transplantation is unique as it involves healthy individuals who undergo a major surgery. This retrospective study seeks to investigate the effect of donor–recipient relationship on postoperative outcomes in Turkish donors undergoing laparoscopic donor nephrectomy (DNx). Material and Methods: The study was conducted with a total of 297 patients who underwent left DNx. The patients included in the study were divided into six different groups based on the degree of relationship with the recipients: Sixty-nine cases of DNx involved mothers as kidney donors classified into group-1, 29 cases involving fathers into group-2, 70 cases involving spouses into group-3, 68 cases involving siblings into group-4, 31 cases involving children into group-5, and 30 cases involving second-degree and more distant relatives into group-6. Patients’ data including age, sex, education level, duration of surgery (ST), Visual Analog Scale (VAS) pain score at postoperative day 1, length of hospital stay (HS), and Quality of Life (QoL) were retrospectively analyzed and recorded. Results: The groups had significant differences in terms of VAS scores, HS, and QoL-MS. Posthoc analysis was performed to find out which groups had significant differences. Results showed that group-1 had significantly lower VAS scores than group-2, group-3, and group-6. HS was significantly long in group-3 and group-6. QoL-MSwas significantly lower in group-2 and group-6 than the other groups. Conclusion: The degree of relationship of living kidney donors to recipients influences their psychological health in the early postoperative period and probably affects VAS scores and length of hospital stay. It can be argued that mothers are the group of donors least affected by the kidney donation process. Keywords: kidney; transplantation; live donor; donor-recipient relationship

Struggle with adversity through life: factors influencing the level of resilience in adolescents and its enhancement pathways

Resilience refers to a person's psychological toughness, which is the ability to coordinate and adapt well in the face of adversity, and is mainly formed during adolescence. Also, it is significant for later adaptation and development in difficult or high-risk environments. In this paper, through document analysis and interviews, we found that the factors affecting the level of resilience of adolescents mainly include: individual negative adversity perceptions and behavioral responses; distant parent-child relationships, abusive behaviors and negative family events in the family; the quality of the school and community environment to be improved. To address this, the possible pathways of improvement are: to develop intervention plans for individuals and pay attention to adolescents' psychological health; to give full play to the supportive and protective functions of families and carry out parenting function services; to improve the quality of school and community environments and build good social support mechanisms to stimulate adolescents' endogenous motivation and promote healthy development.

Mąż córki, wspólnik teściów czy zagrożenie dla rodziny? O roli zięcia w lwowskiej rodzinie mieszczańskiej na podstawie testamentów z lat 1541–1560

A husband of the daughter, an associate of the in-laws or a threat to the family? On the role of the son-in-law in burgher families on the basis of testaments from 1541–1560 In the article the author examines questions relating to the position of sons-in-law in the early modern burgher families in Lviv. The conclusions of the article are based on sixteenth-century wills. As ego documents — personal documents — the wills contain information about private relations with the various relatives, serving as an irreplaceable source for research into the norms of family life. The problem of the relations between sons-in-law and the family of their spouses has not been previously tackled in the literature on the subject. Most authors have focused on analyses of the functioning of molecular families, leaving aside the relations between more distant relatives by blood or marriage. Yet sons-in-law are, apart from spouses and children, the family members most often encountered in last wills. The author’s analysis of the wills indicates that neither betrothal, nor marriage or payment of dowry put an end to contacts between the son-in-law and the in-laws. One the contrary, in burgher families sons-in-law often became associates in economic activities, representing the interests of not just their wives, but also their in-laws. At the same time, it seems significant that husbands of daughters were best captured in wills where there were no adult male descendants in the family, which enabled them, to some extent, to assume filial powers and privileges. At the same time, the son-in-law’s high position within the family may have made his father-in- law — the testator — who was in conflict with him fear for the future of both his widowed wife and his orphaned children. Thus the position of the daughters’ husbands was not strictly defined by custom, and their role as well as influence in their spouses’ families depended on various factors, including whether the family was part of urban society, whether there existed personal ties or other male relatives.

Isolation, culture and characterization of Arsenophonus symbionts from two insect species reveal loss of infectious transmission and extended host range.

Vertically transmitted "Heritable" microbial symbionts represent an important component of the biology and ecology of invertebrates. These symbioses evolved originally from ones where infection/acquisition processes occurred within the environment (horizontal transmission). However, the pattern of evolution that follows transition from horizontal to vertical transmission is commonly obscured by the distant relationship between microbes with differing transmission modes. In contrast, the genus Arsenophonus provides an opportunity to investigate these processes with clarity, as it includes members that are obligate vertically transmitted symbionts, facultative vertically transmitted symbionts, strains with mixed modes of transmission and ones that are purely horizontally transmitted. Significantly, some of the strains are culturable and amenable to genetic analysis. We first report the isolation of Arsenophonus nasoniae strain aPv into culture from the ectoparasitic wasp Pachycrepoideus vindemmiae and characterize the symbiosis. We demonstrate maternal vertical transmission and find no evidence for paternal inheritance, horizontal transmission or reproductive parasitism phenotypes. This leads us to conclude this strain, in contrast to related strains, is a facultative heritable symbiont which is likely to be beneficial. We then report the serendipitous discovery and onward culture of a strain of Arsenophonus (strain aPb) from the blue butterfly, Polyommatus bellargus. This association extends the range of host species carrying Arsenophonus nasoniae/Arsenophonus apicola symbionts beyond the Hymenoptera for the first time. We perform basic metabolic analysis of the isolated strains using Biolog plates. This analysis indicates all strains utilize a restricted range of carbon sources, but these restrictions are particularly pronounced in the A. nasoniae aPv strain that is solely vertically transmitted. Finally, we demonstrate the Arsenophonus sp. strain aPb from the blue butterfly can infect Galleria waxworms, providing a model system for investigating the functional genetics of Arsenophonus-insect interactions. These results are consistent with a model of reduced metabolic competence in strains evolving under vertical transmission only. The data also broadens the range of host species infected with nasoniae/apicola clade strains beyond the Hymenoptera, and indicate the potential utility of the Galleria model for investigation of symbiosis mechanism.

Genetic and genealogical (genealogical) study of the genus Nilogovoi (based on the DNA of autosomes)

The article deals with the problem of genetic and genealogical (genealogical) verification of the documentary pedigree of the Nilogovs, reconstructed by the methods of classical genealogy. Thanks to the testing of male and female representatives of the genus Nilogov on the DNA of autosomes, it was possible to genealogically confirm the documentary pedigree of the author of the article to his direct ancestor Semyon Danilovich Nilogov, who lived in the XVIII century. The object of study is the biological pedigree of the Nilogovs, and the subject is the archival and documentary reconstruction of the pedigree of the Nilogovs before the XVIII century. The source base of the work was the collections of such archives as: the State Archive of the Perm Territory, the Komi-Permyatsky District State Archive, the Russian State Archive of Ancient Acts, the archive of the city of Minusinsk; personal archives of several Nilogov families were also used. The novelty of the work is the application of modern genetic and genealogical methodology for the objectification of a specific documentary pedigree by certifying biological kinship in the "descendant – ancestor" chain. Specifically, the genetic relationship of two distant relatives N. A. Nelogov and R. A. Arinicheva was studied at the level of the sixth degree. Thanks to testing of autosomal DNA of these donors, it was possible to identify their common nucleotide segments (text sequences in the form of nitrogenous bases), after which, using the statistical database of genetic kinship, The Shared cM Project 4.0., it was possible to confirm the documented reconstructed pedigree. The introduction of modern methods of molecular genetics (biological science) into scientific genealogy (historical science) is an actual trend of systemic (interdisciplinary) research.

In Silico Characterization of Lycopene Beta Cyclase (LCYB) and Lycopene Epsilon Cyclase (LCYE) Genes from DH-Pahang (Musa acuminata, A Genome) and DH-PKW (Musa balbisiana, B Genome)

Alpha (α) and beta (β)-carotene are among the nutritious contents of bananas, with the unique feature of a high ratio of α-/β-carotene. Research on the gene and protein of the key enzymes determining the ratio of α-/β-carotene in bananas, namely lycopene beta cyclase (LCYB) and lycopene epsilon cyclase (LCYE), is currently not well defined. Hence, this study aimed to compare the characters of the LCYB and LCYE genes and their putative proteins from Musa acuminata 'DH-Pahang' and Musa balbisiana 'DH-PKW'. The corresponding nucleotide sequences from both species were aligned to detect similarities in the gene structure. Their protein products were characterized at the primary and tertiary levels. The phylogenetic tree was constructed based on nucleotide and protein sequences. The result showed that the gene structure between these two species is similar in LCYB in chromosome 9 but different in LCYB in chromosome 7 and LCYE. The presence of cis-acting regulatory elements in response to light dominated the 2000 nucleotide region of the 5'UTR of LCYB and LCYE genes in both species. Based on protein alignment and domain analysis, the NADB_Rossmann superfamily domain was detected in both LCYB and LCYE. Alignment of the three-dimensional protein structure showed a significant difference between MaLCYB.c07 and MbLCYB.c07 only. The phylogenetic tree based on protein sequences indicated the distant relationship of MaLCYB.c07 and MbLCYB.c07 with other LCYB ingroup OTUs. The results of this study could provide a molecular basis related to the exploration of bananas as a promising functional food to meet the needs of provitamin A.

The secrets that you keep: Secrets and relationship quality

AbstractResearch indicates that preoccupation with secrets takes a toll on mental health. There is also some evidence to suggest that keeping secrets from one's romantic partner may harm the relationship as it implies a lack of trust that one's partner will be supportive. Two 4‐wave longitudinal surveys of 609 adults (34.3% men, 65.4% women; Mage = 36.4) keeping a secret from their romantic partner was used to assess the lagged effect of preoccupation with the secret on subsequent relationship satisfaction, trust, and emotional intimacy as well as the reverse. Cross‐lagged panel modeling with random intercepts indicated that whereas individuals in poorer relationships were more preoccupied with their secrets, there was no evidence for cross‐lagged effects. The findings suggest that secrecy may be a symptom—not a cause—of a dissatisfying, distrustful and distant relationship.

Fusceulimoides kohtsukai gen. et sp. nov., a Minute Eulimid Gastropod Parasitic on the Little Brittle Star Ophiactis savignyi in Central Japan.

Gastropods of over a dozen genera in the family Eulimidae have been identified as parasites of brittle stars, and many more remain to be discovered and described for a comprehensive understanding of the evolutionary history of their host-parasite relationships. In this study, we describe Fusceulimoides kohtsukai gen. et sp. nov., parasitic on the little brittle star, Ophiactis savignyi (Ophiactidae), in Kanagawa, central Japan. The new genus is distinguished from other eulimid genera by the combination of following seven conchological characters: (1) a very small size of up to 1.7 mm high, (2) a colorless translucent appearance, (3) a conical pupiform shape with a paucispiral protoconch, (4) slightly convex teleoconch whorls, (5) a remarkably large body whorl occupying 65-70% of the total shell height, (6) a broad, somewhat squarish and laterally expanded aperture with a strongly curved outer lip, and (7) a developed parietal callus without an indentation or depression in the umbilical area. A multi-locus molecular phylogeny revealed its distant relationship to Hemiliostraca + Pyramidelloides, a previously known clade of ophiuroid parasites, thereby suggesting multiple origins of this host-parasite association in Eulimidae.

Kinship analysis of skeletal remains from the Middle Ages

Medieval cemeteries Klisa-Guca Gora, Alihodze and Glavica-Han Bila located in the Travnik area (Travnik, Bosnia and Herzegovina) were archaeologically examined in the period 2011–2014, revealing human skeletal remains of 11 individuals in total. Archaeological skeletal samples, previously deposited in Travnik Homeland Museum (Travnik, Bosnia and Herzegovina) were subjected to genetic analysis. The aim of this research was to test familiar relationship of 11 individuals excavated from three medieval cemeteries and to predict Y-haplogroup for male individuals. In order to perform molecular-genetic characterisation of collected human skeletal remains, two systems of genetic markers were analysed: autosomal and Y-STR loci. Complete or partial data obtained by autosomal STR typing of 11 individuals were subjected to kinship analysis. Male sex was determined in eight samples out of 11. Direct relatives of the "brother-brother" type were detected in one case with high kinship probability (KP) value of 99.99996 %. Complete or nearly complete and usable Y-STR profiles were obtained for six out of eight male individuals. The presence of identical haplotypes at Y-STR loci and results of Y-haplogroup prediction suggest that all male individuals share the same paternal lineage and belong to J2a haplogroup. Overall, this study emphasises the usefulness, efficiency and sensitivity of STR markers in the molecular-genetic characterisation of old skeletal remains as well as the importance of employing additional markers like Y-STRs in archaeogenetic studies, besides traditionally used autosomal STR markers, in order to get a comprehensive information about close and distant relatives, and ancestry.

Close and distant relationships exclusion: A study of individual coping tendencies towards excluder and non-excluder

Close and distant relationships exclusion: A study of individual coping tendencies towards excluder and non-excluder

Mixed heritage, mixed feelings: psychoanalytic parent infant psychotherapy during the coronavirus pandemic

ABSTRACT This paper is an account of Psychoanalytic Parent Infant Psychotherapy (PPIP) by a white therapist with a mixed heritage family during the coronavirus pandemic. It describes changes to the distant relationship between a mother and her infant son who appears at first to be developing an avoidant, dissociated defensive strategy to ward off painful projections from his traumatised parent. Necessary modifications in treatment due to working remotely contributed to several technical adjustments made. The paper attempts to consider the inclusion of race as a fully integrated aspect of working in a transcultural field, taking into account the ‘ghosts in society’. The specific trauma of racist abuse, with an emphasis on colourism that can be experienced by mixed heritage families is discussed. The key to the improved relationship between child and mother by the end of the Parent Infant Psychotherapy is postulated to have come from reflection on the therapist’s countertransference in regard to racism, that then enabled both therapist and patient/s to recognise and begin to work through experiences, thoughts and fantasies about belonging, heritage and racism.

Reinforcement learning-based distant supervision relation extraction for fault diagnosis knowledge graph construction under industry 4.0

Fault diagnosis is the key concern in the operation and maintenance of industrial assets. A fault diagnosis knowledge graph (KG) can provide decision support to the engineers to efficiently conduct maintenance tasks. However, as a type of domain KG, it would be time-consuming to manually label the corpus collected from the multi-source including the maintenance log, handbook and article. Meanwhile, the existence of the noisy sentence in the multi-source corpus jeopardises the performance of relation extraction modelling. In order to address this issue, this paper proposes a distant supervision relation extraction (DSRE)-based approach to construct a fault diagnosis KG. In this approach, the ontology of the fault diagnosis KG is firstly designed. Subsequently, a DSRE algorithm named relation-aware-based sentence-level attention enhanced piecewise convolutional neural network with reinforcement learning strategy (PCNN-ATTRA-RL) is proposed. The algorithm can effectively lower the impact of noisy sentences and accurately label the relation of different entities when the labelled data is insufficient. In this algorithm, PCNN-ATTRA is designed as the DSRE classifier to effectively extract the relation between entity pairs. RL is conducted to remove the noisy sentence so as to further improve the performance. An experimental study based on the multi-source corpus collected from the real world reveals that the proposed approach shows merits in comparison with the state-of-the-art algorithms. Meanwhile, a fault diagnosis KG, which can greatly support the decision-making of the engineers in the fault diagnosis, is established via the proposed approach.

Sexuality and affectivity after a grieving process for an antenatal death: a qualitative study of fathers' experiences

The loss of an infant at the prenatal stage is one of the most traumatic events parents can experience. Prenatal losses have several negative implications for parents’ physical, psychological, and social well-being, including intimacy and sexuality. Fathers who suffer from this experience have to cope not only with their grief, but also with the physical and emotional suffering of their partners. The social context gives the father a masculine role of strength, insensitivity, and protection of the mother, with the result that his pain and grief become invisible. The objective of this study is to understand fathers’ experience of affective-sexual relationships after a grieving process for an antenatal death; A qualitative study based on interviews with 11 fathers in Spain who have experienced an antenatal death was conducted. Data were analyzed with the help of ATLAS.ti software to discover emerging themes. 6 sub-themes were developed from the analysis, grouped into two main themes: the invisibility of grieving fathers and the relationships between the grieving parents are influenced by the death of their infants. The sexuality of fathers who suffer an antenatal death is altered. Gender stereotypes and the lack of social and professional awareness make their grief invisible. Fathers need to express their emotions to cope with their own grief and break the stereotypical gendered bereavement. In most cases, the couple’s relationship is altered, from a close union to a more distant relationship, in addition to a decrease in sexual desire and arousal. However, other fathers experienced greater closeness and intimacy in the couple. A communication based on sincerity, exposing their own grief, feelings, emotions and needs could help the couple’s relationship.