The purpose of this study was to evaluate the influence of heat treatment on the microstructural change and low cycle fatigue (LCF) resistance of an electron beam welded (EBWed) dissimilar joint between Ti-6Al-4V and Ti17 alloys. The aging with solution (STA) had a more significant effect on the microstructure and hardness than aging, compared to the as-welded joint. The post-welded joints in both aging and STA conditions were basically cyclic stable at low strain amplitudes up to 0.6%, while cyclic softening occurred at higher strain amplitudes. The fatigue life in the aging condition was slightly longer than that in the STA condition at the lower strain amplitudes. Fatigue crack initiation occurred from the specimen surface or near-surface defect, and fatigue crack propagation was characterized mainly by the fatigue striations coupled with secondary cracks in both aging and STA conditions.