Disk Test Research Articles

Detection of Carbapenem Resistance Using the Genotypic and Phenotypic Methods in Klebsiella pneumoniae

Aim: This study aimed to detect the carbapenem resistance of the Klebsiella pneumoniae strains, isolated from clinical specimens with genotypic and phenotypic methods. Material and Methods: A total of 87 Klebsiella pneumoniae strains whose carbapenem resistance was determined by disc diffusion method were included in the study. Carbapenemase was investigated using the combined disk method and polymerase chain reaction (PCR). Results: The evaluation of the PCR results demonstrated that OXA was detected in 60 (68.9%) samples, NDM was detected in 20 (22.9%), OXA + NDM in 5 (5.7%), and KPC was detected in 1 (1.1%) out of 87 clinical samples. Carbapenemase was not detected in one specimen with the PCR method. The results were found compatible with the combined disc test results for all isolates which were detected as only OXA, NDM, and KPC type carbapenemase positive. In 5 (5.7%) strains in which the co-existence of NDM and OXA type carbapenemases was detected by PCR, the combined disc method detected only OXA type carbapenemase. Conclusion: The combined disk method is inadequate in the presence of strains that have multiple carbapenemases, and also have OXA which is the most frequently detected carbapenemase in our hospital. EUCAST recommends verification by other methods in the presence of OXA-48. Genotypic methods can be used for confirmation testing. The detections of strains with NDM, multiple carbapenemases, and the first detection of KPC were striking in the study. Monitoring the spread of these strains in the hospital will be necessary for infection control.

Antimicrobial activity of cefepime-tazobactam combination against extended spectrum beta-lactamase and/or AmpC beta-lactamase- producing gram-negative bacilli

BackgroundThe problem of resistance to beta-lactam antibiotics, which is caused by ESBL and AmpC β-lactamases, is getting worse globally. Infections caused by bacterial isolates harboring these enzymes are difficult to treat with carbapenems being the sole effective treatment option for such infections. The objective of this study was to determine the frequency of ESBLs and AmpC-producing Gram-negative bacilli isolated from clinical specimens and to evaluate the sensitivity of cefepime-tazobactam combination against them.MethodsThis is an observational cross-sectional study carried out on 100 Gram-negative bacilli at Theodor Bilharz Research Institute Hospital during the period from February 2015 to January 2016. ESBL production was screened by using the disc diffusion test followed by confirmation by the combined disc confirmatory test, the screening for AmpC production was conducted using the cefoxitin disc test, which was subsequently confirmed by the AmpC disc test. Isolates confirmed positive for ESBL and/ or AmpC production were investigated for their susceptibility to antibiotics.ResultsAmong 100 Gram-negative bacilli, 44 isolates were confirmed as ESBL producers by the combined disc confirmatory test out of 56 isolates that tested positive for ESBL production through the disc diffusion test. The presence of AmpC production was assessed using the cefoxitin disc test, 32 isolates were screened to be AmpC producers, and the AmpC disc test confirmed AmpC production in 9 isolates of them. Using the Mast® D68C set, 32 isolates were ESBL producers, 3 were AmpC producers, and 4 isolates were ESBL/AmpC co-producers. The highest sensitivity was to cefepime-tazobactam (91.48%) followed by the carbapenems.ConclusionCefepime-tazobactam showed remarkable activity against ESBL and/or AmpC-producing Gram-negative bacilli and may be considered as a therapeutic alternative to carbapenems.

Advanced tensile fracture analysis of alumina ceramics: Integrating hybrid finite-discrete element modeling with experimental insights

This study offers novel insights into the Mode I tensile response of an alumina ceramic through the use of computational modeling and the flattened Brazilian disk (FBD) experiments. A modified hybrid finite-discrete element method (HFDEM) is developed, integrating a coupled damage and friction cohesive model and a microscopic stochastic fracture model with a Weibull strength distribution by Monte Carlo simulation. The model is used to simulate direct tensile failure processes under quasi-static loading conditions, providing qualitative and quantitative predictions of direct tensile failure processes of an alumina ceramic. Concurrently, quasi-static flattened Brazilian disk tests (indirect tensile tests) are performed on a standard MTS machine coupled with a high-speed camera. The modified HFDEM model is also applied to reproduce the FBD experiments, and our simulated tensile strength is consistent with the experimental results. The results of the modified HFDEM model show three kinds of phenomena (i.e., “underestimation”, “reasonable estimation”, and “overestimation” of the indirect tensile strength) and four different types of associated fracture and fragment patterns of FBD testing. The integration of simulation and experimental results reveal relationships between fracture patterns, fragment geometry, tensile strength, and indirect tensile strength. The fracture and fragmentation patterns derived from our modified HFDEM model can be utilized to analyze the “tensile strength” measured in BD testing. Overall, this research offers important insights and direction for future Brazilian disk experiments and tensile strength assessments, enhancing our comprehension of ceramic failure mechanisms under tensile loadings.

Cleanroom Dashboard System for Time-Reduction Checking of Disk Tester Availability

With the vast amount of data being processed and reviewed in production and manufacturing industries nowadays, it takes a lot of time and effort to keep track of their performance metrics. Developing an automation system to improve the overall system performance and reduce man-machine interactions is necessary. Prior to the work, a computer disk drive manufacturing facility checked its fixed-tester availability manually, which made the company unable to optimize its man and machine hours as much as possible. This work proposes a system that displays all the critical information in a dashboard system to address the problem while enabling access to data history logs and further analytic insights. Moreover, the system enables readily accessible fixed-disk tester availability. The outcomes indicate significant improvements, especially in delay reductions, necessary for optimizing man and machine interactions in a fixed-disk testing facility at a leading computer disk drive manufacturer and data storage company at Laguna Technopark.

Effectiveness of Administering Red Belt Leaf Extract (Piper Crocatum) Against Aeromonas Hydrophila Bacteria in Vitro

Diseases caused by pathogenic bacteria such as Aeromonas hydrophila are a major health concern. However, the use of red betel leaves (P. crocatum) can serve as a potential solution to this problem due to their antibacterial properties. This research aimed to study the antibacterial activity of red betel leaf extract (P. crocatum), the structure and character of the bacteria inhibited by the extract, and the bioactive components present in the leaves. The results of the disc test showed that the average zone of inhibition was 14.28 mm, while the MIC test results revealed that a dose of 100 mg/L could inhibit bacterial growth (bacteriostatic). Moreover, the crude extract of red betel leaf (P. crocatum) damaged the cell walls and cell membranes of A. hydrophila bacteria, thereby affecting their character and structure. The UV-VIS analysis of red betel leaf extract (P. crocatum) and FTIR analysis with 96% ethanol absorption indicated the presence of flavonoids, saponins, tannins, and alkaloids. These findings suggest that red betel leaves can be used as a natural remedy to fight bacterial infections caused by pathogenic bacteria such as Aeromonas hydrophila

Novel in-situ real-time line scan optical monitoring of wear and surface damage initiation in a laboratory twin disc test

A new optical monitoring system has been developed to photograph in-situ in real time the initiation of damage on the running surface of a rail steel twin-disc sample undergoing wear testing. The line-scan camera system has been demonstrated on the Sheffield University ROlling Sliding 2 (SUROS2) twin-disc machine. The results show the system can continuously track the development of wear flakes, with wear flake initiation and stabilisation of wear flake size observed without test interruption for the first time. Image analysis to quantify the total wear flake shadow pixel count showed a good correlation with the mass loss results, indicating the potential for the optical data to quantify rail steel wear without interruption to testing. Furthermore, in a water-lubricated test the new system enables observation of rolling contact fatigue (RCF) crack initiation through a water layer present on the specimens, without requiring test interruption. The improving knowledge of the wear and RCF performance of rail steels available from the new observation method can help improve understanding of steel performance and support to the selection of rail steel grades according to their performance.

Molecular Identification of Carbapenem Resistance Acinetobacter baumannii causing Ventilator-Associated Pneumonia Isolated from Intensive Care Unit of Tertiary Care Hospital

Ventilator-associated pneumonia (VAP) is a serious complication in critically ill patients, significantly increasing morbidity and mortality. One concerning organism behind VAP is Acinetobacter baumannii, a multidrug-resistant bacterium has ability to evade treatment, particularly with carbapenems, the last-line antibiotics. This is especially worrisome within the confines of Intensive Care Units (ICUs) of tertiary care hospitals, hubs for high-risk patients and potential reservoirs of antimicrobial resistance. This study focused on identifying carbapenem-resistant Acinetobacter baumannii using both phenotypic and genotypic methods. In 132 isolates of Acinetobacter baumannii, we observed 96% resistance to the cephalosporins while least resistance found to colistin and tigecycline. However, a concerning 51.5% of isolates exhibited carbapenem resistance. Phenotypically confirmation of carbapenem resistance detected in 47% isolates by Combined Disc Test and 51.5% isolates by Modified Hodge Test and E-test. Genotypic analysis with RT-PCR revealed a diverse array of resistance genes: blaIMP (33.82%), blaVIM (25%), blaOXA-Group (20.58%), and blaNDM (8.82%). These findings highlight the alarming prevalence of carbapenem-resistant Acinetobacter baumannii in healthcare settings.

New method for determining the mode I fracture toughness of shotcrete: edge-notched partial disc test

The fracture toughness (mode I) of shotcrete specimens was obtained using edge-notched partial disc (ENPD) specimens. Notched Brazilian discs (NBDs) were also tested to validate the results of the ENPD experiments. Numerical analysis was also conducted on the ENPD results to compare the measured and numerically obtained fracture toughness values. The notch lengths in the ENPD specimens were 15, 30, 45 and 60 mm, while the notch lengths in the NBD specimens were 10, 20, 30, 40, 50 and 60 mm. It was found that the flat joint model accurately predicted the potential crack growth path and crack initiation stress as compared with the experimental results. It was also deduced that the fracture toughness was roughly the same with an increase in the notch length. The tensile strength (σt) and fracture toughness (KIC) of the shotcrete specimens were found to be meaningfully correlated (σt = 7.92KIC). The ENPD tests yielded the lowest fracture toughness values because of the pure tensile stress distribution on the failure surface. It was also found that the derived fracture extension patterns from the laboratory investigations were in acceptable agreement with the outputs of numerical simulations.

Metallo-Beta-Lactamase Producing Isolates of Escherichia coli and Klebsiella pneumoniae and their Resistance Profiles in Enugu, Nigeria: A Threat to Public Health

Background: A potential threat to public health is the rapidly spreading enterobacteriaceae, especially Escherichia coli and Klebsiella pneumoniae which produce metallo-beta-lactamases (MBL). This study evaluated the prevalence of metallo-beta-lactamase (MBL) from clinical and non-clinical sources in Enugu Metropolis.
 Methodology: The study was conducted in the Microbiology Laboratory of the University of Nigeria Teaching Hospital, Ituku-Ozalla between October 2020 and July 2021. A total of 150 isolates including 85 and 65 isolates of Escherichia coli and Klebsiella pneumoniae respectively was recovered. Standard microbiology procedures were used to identify and characterize the isolates. Antimicrobial susceptibility was done using the Kirby-Bauer disc diffusion technique. Phenotypic detection of Metallo-beta-lactamase production was determined using Combined Disk Tests.
 Results: Imipenem resistance was detected in 22 (25.9%) isolates of E. coli and 18 (27.7%) isolates of K. pneumoniae. Of the 22 strains of E. coli that were imipenem resistant, 8 (9.4%) and 14 (16.5%) were found to be MBL producers and non-MBL respectively. Of the 18 strains of Klebsiella pneumoniae that were imipenem resistant, 10 (15.4%) were MBL producers and 8 (12.3%) were non-MBL producers. The highest prevalence of MBL was recovered from urine sources in both E. coli and K. pneumonieae. All MBL-producing isolates were multidrug resistant.
 Conclusion: The overall prevalence of MBL in this study was 12.0%. Public health is at risk due to the occurrence of metallo-beta-lactamase. Antimicrobial stewardship and the implementation of infection control strategies are required to halt the spread of these resistant bacteria in the environment. The use of antibiotics should be with utmost prudence.

Evaluation of Phenotypic Tests for Carbapenemase Detection in Enterobacteriaceae in Tunisia.

Introduction: Resistance to carbapenems in Enterobacteriaceae is a challenge for public health. Carbapenemase production is the leading mechanism. This work aims to evaluate four phenotypic methods for carbapenemase detection in comparison with a molecular method. Materials and Methods: Thirty-seven nonrepeating Enterobacteriaceae strains with decreased susceptibility to ertapenem were included. Imipenem MIC, Modified Hodge Test (MHT), Neo-Rapid Carb Kit® and KPC, MBL, and OXA-48 Confirm Kit® were performed. Isolates were tested for blaOXA-48, blaNDM, and blaVIM genes by end-point polymerase chain reaction. The results of the molecular study were used as a reference test to determine the performances of the phenotypic tests. Results: Imipenem resistance does not seem to be a good marker for carbapenemase production with a sensitivity of 54% (95% CI: 38-71). MHT showed 82% sensitivity (95% CI: 65-91). Overall, the enzymatic test showed the best performances for carbapenemase detection with 100% sensitivity (95% CI: 89-100) and the best turnaround time. The characterization of carbapenemases classes by the combined discs test demonstrated 88% overall sensitivity (95% CI: 72-95). Conclusion: The results of this study support the combination of the enzymatic and the combined disc tests for carbapenemase detection in Enterobacteria.

Phenotypic Detection of Extended Spectrum β-lactamase Producing Gram Negative Bacteria Isolated From Clinical Specimens

Background: Over the past decade, there has been a significant global rise in extended spectrum β-lactamase (ESBL) producing gram negative bacteria (GNB), posing a substantial concern. ESBLs, encoded by plasmids, are deemed threatening due to their ease of transmission between species. The Asia Pacific region has witnessed a surge in hospital-acquired infections attributed to ESBL-producing GNB, as highlighted by numerous studies. This study seeks to assess the prevalence of ESBL-producing GNB specifically within Nepal. Methods: This research, conducted at Grande International Hospital in Kathmandu, Nepal, adopted a descriptive cross-sectional approach. It spanned from July 2016 to February 2017 and encompassed patients receiving care at the hospital. The study involved the handling of samples directed to the Microbiology laboratory for Culture and sensitivity, as well as the identification of isolates and Antibiotic susceptibility testing, following established microbiological protocols. Screening for Multi-Drug resistance (MDR) and Extended Spectrum Beta-Lactamase (ESBL) was conducted for all Escherichia coli and Klebsiella pneumoniae isolates utilizing a combined Disk (CD) test method. Results: Out of 258 Escherichia coli and Klebsiella pneumoniae isolates, 42 isolates of E. coli and 21 isolates of K. pneumoniae were confirmed as ESBL producer. Conclusion: The regular surveillance of hospital-associated infections including monitoring of antimicrobial susceptibility pattern is needed for the formulation of a definite antimicrobial policy.

Molecular Epidemiology of Escherichia coli Resistant to Carbapenems, Fluoroquinolones, and Aminoglycosides Isolated from One of the Largest Hospitals in Vietnam in 2014-2019.

Multidrug-resistant (MDR) Gram-negative bacilli including carbapenem-resistant Gram-negative Enterobacteriaceae (CRE) threaten global health. Little is known, however, about the distribution of antimicrobial resistance genes in MDR isolated from patients in Vietnamese hospitals. In this study, we collected MDR Escherichia coli, defined as E. coli resistance against all fluoroquinolones, aminoglycosides, and carbapenems. This study was designed to clarify the molecular epidemiology of Escherichia coli isolates resistant to carbapenems, fluoroquinolones, and aminoglycosides isolated from patients admitted to one of the largest hospitals in Vietnam in 2014-2019 based on both whole-genome sequencing (WGS) and phenotypic data. Methodology. Sixty-seven Vietnamese isolates screened by drug resistance by the disk test were subjected to WGS, and their sequences were analyzed to determine their multilocus sequence type (MLST), O-types, H-types, distribution of drug resistance genes, plasmid types, pathogenicity islands (PIs), virulence factor distribution, and phylogenetic evolution using the WGS data. Among the STs detected, ST410 was relatively dominant. Dominant O-types and H-types were O102 and H9 and showed some links, such as those between O102 and H8. The most dominant plasmid type and carbapenemase type were 4 and NDM-5, respectively. MLST, O-types, H-types, plasmid types, and types of carbapenemases were very heterogeneous among the isolates, with no clear correlation between them. Dominant plasmid type carrying drug resistance gene was IncQ1_1. The percentage of isolates positive for drug resistance genes, such as anti-beta-lactams and aminoglycosides, was relatively high because the isolates screened were resistant to carbapenems, fluoroquinolones, and aminoglycosides. MDR E. coli isolates isolated at a high-volume Vietnamese hospital were very heterogeneous, suggesting that they were acquired from different sources, including nosocomial infection, animals, and water. Eradication of MDR E. coli from hospitals and other clinical environments is very challenging because a single measure may be ineffective.

Constructing Z-Scheme 3D WO3@Co2SnO4 Heterojunction as Dual-Photocathode for Production of H2O2 and In-Situ Degradation of Organic Pollutants

As photoelectrochemical catalyst material, Z-scheme heterojunction 3D WO3@Co2SnO4 composites were designed through a hydrothermal-calcination method. The morphology and structure were characterized by SEM, EDS, XRD, XPS, DRS, and Mott–Schottky analysis, and the photoelectrochemical properties were explored with the transient photocurrent and electrochemical impedance. The construction of Z-scheme heterojunction markedly heightened the separation efficiency of photogenerated electron-hole pairs of WO3 and enhanced the light absorption intensity, retaining the strong redox ability of the photocatalyst. The 3D WO3@Co2SnO4 was used as a photocathode for production of H2O2. Under the optimal reaction conditions, the yield of H2O2 can reach 1335 μmol·L−1·h−1. The results of free radial capture and rotating disc test revealed the existence of direct one-step two-electron and indirect two-step one-electron oxygen reduction to produce H2O2. Based on the excellent H2O2 production performance of the Z-scheme heterojunction photoelectrocatalytic material, 3D WO3@Co2SnO4 and stainless-steel mesh were used to construct a dual-cathode photoelectric-Fenton system for in-situ degradation of a variety of pollutants in water, such as dye (Methyl orange, Rhodamine B), Tetracycline, sulfamethazine, and ciprofloxacin. The fluorescence spectrophotometry was used to detect hydroxyl radicals with terephthalic acid as a probe. Also, the photocatalytic degradation mechanism was revealed, indicating the dual-cathode photoelectron-Fenton system displayed satisfactory potential on degradation of different types of environmental pollutants. This work provided insights for designing high-activity photoelectrocatalytic materials to produce H2O2 and provided possibility for construction of a photoelectric-Fenton system without extra additions.

A New Brazilian Disc Test Procedure for the Elastic Moduli of Orthotropic C/C-SiC Composites

A New Brazilian Disc Test Procedure for the Elastic Moduli of Orthotropic C/C-SiC Composites

Detection of phylogrouping, adhesin, and extended spectrum β-lactamases genes in hospital acquired uropathogenic Escherichia coli isolates.

It has been interesting to compare the levels of antimicrobial resistance and the virulence characteristics of uropathogenic Escherichia coli (UPEC) strains of certain phylogenetic groups. The purpose of this study was to identify the frequency of phylogenetic groups, adhesin genes, antibiotic sensitivity patterns, and extended spectrum-lactamases (ESBLs) genes in hospital-acquired UPEC. After UPEC isolation, the disc diffusion method was used to assess its susceptibility to antibiotics. Combination disc testing confirmed the existence of ESBL producers. Polymerase chain reaction (PCR) was used to detect genes for adhesin and ESBLs. One hundred and twenty-eight E. coli were isolated which had the highest resistance to tetracycline (96%) followed by cefoxitin (93%), cefepime (92%), ceftazidime (79%), aztreonam (77%) and sulfamethoxazole-trimethoprim (75%). About 57% of isolates were phenotypically ESBLs positive and they were confirmed by PCR. B2 phylogroup (41%) was the most frequent in E. coli isolates then group D (30%), group A (18%), and lastly group B1 (11%). ESBLs genes were more significantly prevalent in phylogroups B2 and D than other phylogroups (P < 0.001). Regarding adhesin genes, both fim H and afa were more significantly associated with group B2 than other groups (P < 0.009, < 0.032), respectively. In ESBL-positive isolates, both genes were more significantly detected compared to negative ones (P < 0.001). Phylogroups B2 and D of UPEC are important reservoirs of antimicrobial resistance and adhesion genes. Detection of ESBL-producing E. coli is important for appropriate treatment as well as for effective infection control in hospitals.

PREVALENCE AND CHARACTERISATION OF EXTENDED-SPECTRUM BETA-LACTAMASES AND PLASMID-MEDIATED QUINOLONES RESISTANCE IN Enterobacteriaceae ISOLATED FROM COMPANION ANIMALS

Antimicrobial resistance is a major public health concern worldwide. This study aims to determine the prevalence of Enterobacterales producing beta-lactamase (TEM, SHV, OXA) or extended-spectrum beta-lactamases (ESBL), as well as plasmid-mediated resistance to quinolones (PMQR) (qnrA, qnrB, qnrS) in companion animals from the northeast region of Romania. A total of 124 faecal samples were collected aseptically from healthy dogs attending the veterinary practice for vaccination and cultivated on Brilliance ESBL medium (Oxoid, UK). The ESBL production testing was performed using the combination disc test. The identification of Enterobacterales strains was achieved using molecular identification and based on biochemical tests. Antimicrobial susceptibility testing was performed using the disk diffusion method. Identification of genes encoding for beta-lactamase enzymes and genes encoding plasmid-mediated resistance to quinolones was performed by PCR according to the protocols previously described. After ESBL screening, 31 (31/124; 25%) extended-spectrum cephalosporin (ESC)-resistant Enterobacterales were obtained, and 67.74% (21/31) of them were confirmed as ESBL-producers. Regarding the Enterobacterales species, 27 (27/31; 87.1%) were Escherichia coli and 4 (4/31; 12.9%) strains were Klebsiella pneumoniae. Among the ESBL-producing isolates, the blaCTX-M-1 gene group was predominant (58.82%), followed by the blaCTX-M-9 group (41.18%). The blaTEM, blaSHV and blaOXA gene groups were identified in 54.83%, 29.03% and 3.22% of the analysed strains, respectively. The prevalence of PMQR genes was 22.58% and consisted only of qnrS (19.35%) and qnrA (3.22%) genes. The prevalence of ESBL strains related to the total number of analysed samples was 16.93% (21/124). The findings show a significant prevalence of ESBLs and PMQR genes in Enterobacterales strains isolated from the faeces of healthy dogs, implying that pets may pose a risk of transmitting ESBL strains to other animals or owners.

Effect of Expansion Agent and Glass Fiber on the Dynamic Splitting Tensile Properties of Seawater–Sea-Sand Concrete

In marine structural engineering, the impact resistance of concrete holds high significance. The determination of whether the combined use of expansion agent (EA) and glass fiber (GF) has a synergistic effect on the impact resistance of seawater–sea-sand concrete (SSC) and plays a role in its performance and application. In this study, the dynamic Brazilian disc test at various strain rates was carried out with an SHPB device to investigate the effect of mixing 0% and 6% EA with 0% and 1% GF on the dynamic splitting tensile properties of SSC. The results show that strain rate effect on EA and GF-reinforced SSC during dynamic splitting tensile tests at higher strain rates, indicating strong strain rate sensitivity. The synergistic reinforcement of EA and GF consumed more energy under impact loading, thus maintaining the morphological integrity of concrete. However, the dynamic splitting tensile strength obtained in the Brazilian disc test had a significant overload effect which cannot be ignored. EA doped at 6% and GF doped at 1% showed a synergistic enhancement of SSC’s dynamic splitting tensile properties.

Finite Element Method analysis application in identifying the causes of brake disc failure

This article presents the results of brake disc tests aimed at identifying the causes of its failure. The first part of the article presents an analysis of the damageability of selected vehicle components, which showed that among the reported failures, the most failure was the braking system. The assessment of the brake disc worn "properly" as a result of operation and the deformed brake disc after a very short period of operation, which was the subject of further analysis. The next part of the article presents issues related to the modeling of thermal loads, and then, trying to assess and search for the cause of abnormal wear of the brake system element, the use of the Finite Element Method for the analysis and assessment of brake disc wear was proposed. In the final part of the article, conclusions and directions for further work were formulated.

Experimental investigation on I-II mixed mode fracture of hot mix asphalt using double-edge notched Brazilian disk test

The I-II mixed mode fracture is a typical failure pattern observed in asphalt pavement. In this study, the fracture behavior under I-II mixed mode of three diverse asphalt mixtures was scrutinized. The investigation involved performing double-edge notched Brazilian disk (DNBD) tests at two different temperatures: −10 °C and 25 °C. DNBD specimens were prepared with two notch lengths (10 mm and 20 mm) and seven notch angles, including 0°, 15°, 30°, 45°, 60°, 75°, and 90°. Stress intensity factor (KIC and KIIC), equivalent stress intensity factor (K*IC and K*IIC), and fracture energy were used to characterize the fracture behaviors. The findings suggest that the generation of mixed-mode fractures can be manipulated through variations in notch length and angle. An increase in notch angle shifted the fracture behavior towards a more mode I-dominant pattern, while elongating the notch length amplified the significance of mode II fracture contributions to the overall fracture process. The influence of reclaimed asphalt pavement (RAP) on the fracture performance of asphalt mixtures exhibited variation across the temperatures ranging from −10 °C to 25 °C. At −10 °C, mixture C, which contained an SBS-modified binder, demonstrated the most robust fracture performance, as evidenced by higher values of KIC and KIIC. This was succeeded by mixture B that used a #70 binder, with mixture A incorporating RAP trailing behind. Conversely, at 25 °C, the presence of RAP contributed to achieving the highest indices of K*IC and K*IIC, surpassing even the performance of mixture C with its SBS-modified binder, followed by mixture B with its #70 binder. For specimens with identical notch lengths and angles, the stress intensity factors recorded at −10 °C were substantially higher compared to those at 25 °C. This observation suggests that fracture resistance diminishes as temperature rises. Furthermore, an increase in notch angle correlated with a reduction in fracture energy at −10 °C, whereas at 25 °C, the notch angle’s influence on fracture energy appeared to be negligible. When examining the performance at −10 °C, mixture C exhibited the highest fracture energy, with mixtures B and A following in descending order. In contrast, at 25 °C, mixture A outperformed mixtures C and B in terms of fracture resistance, as gauged by fracture energy. Notably, the effect of mixture type on fracture energy was consistent with the effect of mixture type on stress intensity factor.

Effect of isothermal temperature and load on wear of nanobainitic cast steel

The wear behaviour of a nanobainitic cast steel: 0.76C-2.08Si-0.74Mn-0.79Cr (wt. %), isothermally treated at different temperatures, 250 and 300°C; have been investigated by pin on disk test in dry conditions. The tests were carried out at different loads: 2, 7, 12 and 15 N. The results show that the wear rate is higher when the isothermal transformation temperature and load increases. Also, at low loads adhesion, oxidation and abrasion are the main wearing mechanisms, while at high loads contact fatigue becomes dominant. The results are related to the microstructural differences produced by the change in the isothermal temperature of the heat treatment.