Disinfection Measures Research Articles

Research on the "DIY" Innovation Mode of College Students' Self-service Kitchen

The "DIY" innovative self-service kitchen for college students, on the basis of sufficient research, fully grasps the consumption psychology and habits of college students, and builds a practical self-service kitchen development plan. The project is in line with the state's advocacy of enriching college students' spare time, mastering certain labor skills, meeting the needs of college students' experience consumption, and at the same time driving the upgrading and development of the catering industry. The project has a fully equipped kitchen and is equipped with a comfortable dining unit. It is suitable for weekend roommate gatherings, class reunions, and couples to make delicious food. If there is a need in the process of making food for a dinner party, we will provide professional cooking guidance. We have rich practical experience in project management, are responsible for publicizing services such as order taking, and can ensure the implementation of food safety and disinfection measures in terms of internal hygiene and safety.

Some topical issues of prevention of salmonella infection in the production and consumption of chicken eggs: the role of disinfection in the context of antiepidemic measures

Chicken eggs and their processed products are food products that have valuable nutritional and dietary properties.However, the shell and the food eggs themselves can be infected with Salmonella pathogens — Salmonella Enteritidis and Salmonella Typhimurium. The prevalence of salmonella infection associated with the production and consumption of chicken eggs, economic losses from the diagnosis and treatment of patients predetermine the need for a comprehensive study of risk factors, the development and improvement of a set of measures to prevent this anthropozoon infection. An analytical review of the scientific literature and regulatory documents allowed us to conclude that for the prevention of salmonellosis, it is necessary to develop and implement a set of sanitary and veterinary measures aimed at breaking the vertical and horizontal transmission routes of infection, covering all links of the food chain (from laying hens to eating eggs), among which disinfection measures play an important role.

Dry-fog disinfection as a method for processing vehicles for passenger transport

Abstract Due to the corona pandemic, the demand for efficient and fast-acting disinfection measures is high. In this work, the disinfecting potential of a dry fogging technology (Apollon Biotech GmbH, Germany) was investigated for the possibility of disinfection of vehicles for passenger transport. This could represent a new approach for processing emergency vehicles or local passenger transport vehicles quickly and cost-effectively. A public bus was used as a model for a passenger transport vehicle (Ferienfahrschule Hense GbR, Germany). This vehicle was tested at predetermined points before and after dry-fog disinfection with swab and contact plate samples. In addition, contaminated sample tiles (Staphylococcus aureus ATCC 6538; 5x104 CFU) were placed at some locations in order to be able to record a standardized germ reduction. To determine the decontamination effect of the air in the vehicle, active airborne germ measurements were performed. In the airborne germ measurement, our results indicated a reduction of approx. 68% of the microbiological air contamination. Screening of the total contamination of the bus at several selected sites showed an average load of approximately 348 CFU per test site. After disinfection, the average bacterial count was reduced to approx. 18 CFU per test site. This disinfecting effect could also be shown with the standardized test tiles. An efficiency level of between log 0.8 and 3.7 was obtained at the various test sites.

Whole genome sequencing of <i>Acinetobacter baumannii</i> strains isolated from hospital patients in the northern territories of the Tyumen region

Introduction. is to The analysis of the genetic relatedness of isolates aiming to find the source of infection is an important task of nosocomial infection control. The most common causative agent of healthcare-associated infections is Acinetobacter baumannii.Objective. To evaluate the results of whole genome sequencing of A. baumannii bacteria isolated from clinical samples of patients undergoing inpatient treatment in the northern territories of the Tyumen region.Materials and methods. Nine isolates of A. baumannii from the clinical material of patients were studied. Bacterial cultures were identified by mass spectrometry. Whole genome sequencing, multilocus sequence typing and search for markers of antibiotic resistance were performed.Results. The studied strains belonged to sequence types ST2 and ST187, and to the international clonal complex CC2. All A. baumannii isolates were found to have beta-lactamase genes, as well as genes for resistance to aminoglycosides, to the MLS group of antibiotics, and to tetracyclines. The presence of a cluster of genes associated with virulence was detected: those responsible for the synthesis of acinetobactin and iron binding, surface antigen 1 and porin.Conclusion. Based on data of a single nucleotide polymorphism (SNP) analysis, A. baumannii isolates from the clinical material of patients of healthcare institution #1 belong mainly to one bacterial strain. Isolates of A. baumannii from the clinical material of patients of healthcare institution #2 are closely related. The ability to distinguish clinical isolates of A. baumannii at the level of several SNPs per genome will improve the identification of the source of infection, and whole genome sequencing data can contribute to the rational prescription of antibiotic therapy and the correction of disinfection and antiseptic measures.

Comparison of Er:YAG laser and ultrasonic in root canal disinfection under minimally invasive access cavity.

The disinfection of root canal through minimally invasive access cavity remains questionable. This in vitro study compared the effectiveness of three disinfection measures including conventional irrigation, ultrasonic assisted irrigation, and erbium:yttrium-aluminum-garnet (Er:YAG) laser assisted irrigation through conventionally or minimally invasive access. Sixty-six extracted maxillary first molars were randomly divided into group 1 conventionally invasive access group (CIA) and group 2 computer-guided minimally invasive access group (MIA). Each group was further randomly divided into three subgroups, (A) conventional irrigation (CI), (B) passive ultrasonic agitation (PUI), and (C) Er:YAG laser activated irrigation (LAI). Enterococcus faecalis (E. faecalis) infection model was established inside all root canals after instrumentation was performed up to ProTaper Universal F2. After various disinfection methods, microbial samples were collected from root canals by paper tip method and cultured, and colony forming units (CFU) values of each sample were calculated. Then the root canals were enlarged to the size of F3, after which dentin debris was collected from the F3 file. After dilution and culturing, the CFU value was calculated for each group. Two-way analysis of variance (ANOVA) was performed to test the interaction. The results revealed a significant antagonism (F = 3.394, P = 0.043). The bacterial CFU counts of group B and group C were significantly less than that of group A (P < 0.05), and there was no significant difference between group B and C (P > 0.05). Additionally, group 2A was better than group 1A (P < 0.05); there was no significant difference between group 1B and group 2B, group 1C and group 2C (P > 0.05). Comparison of the bacterial CFU counts in dentin debris after disinfection, the results revealed a significant antagonism (F = 7.224, P = 0.002), and group C had the least. The disinfection effect of Er:YAG laser or ultrasonic assisted computer-guided minimally invasive access is similar to conventionally invasive access, and Er:YAG laser is better than ultrasonic in removing bacteria from dentinal tubules and is easy to operate, which is more suitable for minimally invasive root canal treatment.

Stenotrophomonas maltophilia outbreak with a commercial blood gas injector as the culprit and interventions for source and prevention: A possible passage between patient and ECMO water heater device

Despite low virulence of Stenotrophomonas maltophilia, it represents one of the leading drug-resistant bacteria. We report a large outbreak of S. maltophilia infection associated with an unexpected source, which turned out to be a commercial needleless blood gas injector. Over a period from January 1 to December10, 2021, 113 patients were identified to have S. maltophilia infection as documented by positive cultures from the clinical samples, extracorporeal membrane oxygenation (ECMO) water heater devices and commercial needleless blood gas injectors. Sixty-seven isolates (59 clinical, 4 ECMO, 4 blood gas injectors) were sent for molecular analysis. Both arbitrarily primed polymerase chain reaction and pulsed-field gel electrophoresis analyses showed 12 distinct genotypes. Of 67 isolates, 58 were clonally related (86.6%), with 52 indistinguishable strains from 4 blood gas needleless injectors, 46 patients' samples (78%), and 2 ECMO samples (50%). Two ECMO samples and 1 clinical sample were clonally identical. In the event that eradication of infections would not be possible despite taking all environmental disinfection measures including the ECMO devices, unexpected sources, such as a commercial needleless blood gas injector, should not be omitted from the list for surveillance. In addition, obtaining surveillance cultures of ECMO water reservoirs should be placed in the routine clinical practice.

Aspectos relevantes de las epidemias y pandemias en México: desde la perspectiva del derecho sanitario

Throughout history, Mexico has been affected by major epidemics and pandemics, for this reason the health system of each era has had to develop, adopt and perpetuate multiple actions and health security measures, in which the following measures stand out: vaccination, adequate personal hygiene, a balanced diet, water chlorination, suspension of some activities, adequate disinfection of public places, preventive and disinfection measures in homes, quarantine, isolation or confinement, correct use of face masks, avoid crowded places, prohibit or avoid collective meetings, this just to mention the most relevant. The aforementioned cares have been implemented with the intention of mitigating and eradicating its spread, as well as reducing its morbidity and mortality from it. The COVID-19 pandemic, like other pandemics that have arisen over time in México, have affected various aspects such as economic, social, political and especially sanitary health. As time has passed, the crisis that the health sector is going through has become evident, which has forced the corresponding health authorities to make multiple efforts to face it and try to cope with these epidemics and pandemics. In this article we make a brief account of the epidemics and pandemics that have affected Mexico from the year 1450 to 2020, as well as the historical evolution of the pertinent health authorities and the general aspects of health law. Keywords: Health law; epidemic; pandemic; preventive medicine; COVID-19.

COVID-19 Outbreak at Sports Club: Conditions of Occurrence and Causes of the Spread of Infection

Relevance. The new coronavirus infection (COVID-19), which appeared in late 2019 in China, has spread to almost all countries of the world in just a few months. The explosive nature of its spread was accompanied by the formation of large epidemic foci in organizations of various profiles, including leisure and sports. Aims. To establish the conditions and causes of the spread of SARS-CoV-2 among the members of one of the sports clubs based on an in-depth epidemiological analysis. Materials and methods. To study the features of the spread of the SARS-CoV-2 virus in a sports organization, the following documents were used previously developed by the authors and successfully tested in practice: «Act of epidemiological investigation of group and outbreak morbidity of new coronavirus infection (COVID-19) at an enterprise/organization/institution» and «Individual card of a patient with a new coronavirus infection (COVID-19) at the enterprise / organization/institution». In the process of epidemiological investigation, in order to detect SARS-CoV-2 RNA in PCR, a laboratory examination of sports club participants (sick and contact) was conducted, followed by genome-wide sequencing of isolated SARS-CoV-2 viruses on the basis of the Laboratory of Molecular Virology of the A. A. Smorodintsev Influenza Research Institute, which performs these types of studies. Results. Within 17 days, 26 cases of COVID- 19 were registered among the sports team members and staff from the support group (coaching staff, medical staff, administrators), which was 74.3% of their actual number. The majority of patients (76.9%) had mild acute respiratory infection, two (7.7%) had no symptoms, and four (15.4%) had interstitial pneumonia. Of the clinical manifestations of the disease, the most frequent were weakness, fever, headache, muscle and joint pain, difficulty in nasal breathing and serous-mucous discharge from the nose, sore throat, cough, shortness of breath, anosmia and dyspeptic manifestations in the form of diarrhea, nausea or vomiting. The occurrence of the outbreak was the result of the introduction of infection from the opposing team at the tournament. The leading factors that contributed to the spread of COVID-19 among sports club members were the admission to games and training of athletes with acute respiratory infections, prolonged close contact between players during training and competitions, violations in the use of personal protective equipment, compliance with hygiene and hand antiseptics, disinfection measures in the premises of sports institutions and defects in the implementation of the regulations for the examination of teams for SARS-CoV-2 during tournaments. Conclusion. Based on the results of the study, data were obtained on the features of the spread of SARS-CoV-2 in sports organizations, which can be used in conducting preventive and anti-epidemic measures in sports and leisure institutions.

ORGANIZATION OF DISINFECTION AND STERILIZATION MEASURES IN MEDICAL ORGANIZATIONS OF THE URAL AND SIBERIAN FEDERAL DISTRICTS IN 2021. NEWSLETTER

The newsletter presents an analysis of data on the organization of disinfection and sterilization measures in medical organizations of the Ural and Siberian Federal districts based on data from the Federal Statistical Observation Form № 27 "Information on disinfection activities" for 2021. A number of indicators are presented in dynamics over 7 years and in comparison with indicators for the Russian Federation. The newsletter is intended for employees of Rospotrebnadzor bodies and organizations, medical organizations, educational organizations, executive authorities in the field of healthcare and other interested persons.

Clinical Presentation and Outcome of Covid-19 Infection in Patients Undergoing Maintenance Hemodialysis

Background: WHO declared Covid-19 a pandemic disease; although 80% of patients with CoV-2 infection have no or mild symptoms, the remaining 20% of patients develop complications. In patients undergoing dialysis, mortality could be higher than that in the general population. This study was conducted to assess the frequency, clinical presentation, and outcome of COVID-19 infection in patients of end-stage renal disease (ESRD) on maintenance hemodialysis.&#x0D; Methodology: This Cross-sectional study was conducted at Department of Nephrology, Jinnah Postgraduate Medical Centre, Karachi, from April to September 2020. This study included 130 patients of either gender of End-Stage Renal Disease on maintenance hemodialysis for at least 3 months. Two Samples for COVID-19 PCR were taken through nasal swab. Blood samples were collected for hemoglobin level, total and differential leucocyte count, platelet count, intact Parathyroid hormone, vitamin D level, Urea, Creatinine, ferritin, transferrin saturation, procalcitonin, D-Dimer, C-reactive proteins, and lactate dehydrogenase. Radiological assessment was done by X-ray Chest. Urea reduction ratio and single pool Kt/V was used for assessment of adequacy of hemodialysis.&#x0D; Results: Out of 130 patients on hemodialysis, COVID-19 was detected in 52 patients, of which males were 69.2% and females were 30.8%. The most common presentation was shortness of breath (28.8%), followed by cough (19.2%). Home isolation was advised to 38.5% while noninvasive ventilation was given to 34.6%. Mechanically ventilated patients were 26.9%. There were 63.5% patients who recovered and were discharged from the hospital or home-isolated, while 19 patients died (36.5%).&#x0D; Conclusion: COVID-19 pandemic has high chances of infection with increased mortality in people with chronic diseases, especially those having ESRD on hemodialysis. This spreading of infection can be hampered with standard measures of disinfection in centers, social distancing in patients, and the use of a face mask.&#x0D; Keywords: Covid-19, Cough, Hemodialysis, Home isolation, Ventilation&#x0D;

Microbiological evaluation of stethoscopes used in the clinical routine of Veterinarians in the Metropolitan Region of Recife, Pernambuco, Brazil

We evaluated the level of contamination of stethoscopes used in the clinical routine of veterinarians. Specimens from 15 stethoscopes were cultured and analyzed before (M0) and after (M1) disinfecting the stethoscope diaphragm with alcohol at 70ºGL. At moment M0, bacterial growth was observed in 73.33% (11/15) of the stethoscopes. Seventeen strains were isolated, and the majority (41.2%, 7/17) were Bacillus spp. followed by Staphylococcus spp. (35.3%, 6/17) and Micrococcus spp. (23.5%, 4/17). In samples collected after disinfection with alcohol (M1), only Bacillus spp. were isolated in 13.3% (2/15) of the stethoscopes. Coagulase phenotypic testing of the Staphylococcus spp. revealed that all the strains were negative. Polymerase chain reaction was performed for the mecA resistance gene in coagulase-negative Staphylococcus strains. Amplification of mecA gene was observed in only 16.66% (1/6) of the samples. Stethoscopes are instruments that transfer pathogens, especially bacteria that may carry resistance genes. However, simple cleaning and disinfection measures in the veterinary medical clinic routine reduce contamination and minimize the risk of spread of resistant microorganisms to animals and the environment.

Оценка эффективности ПЦР тест-систем для контроля деконтаминации объектов окружающей среды, потенциально инфицированных коронавирусом SARS-CoV-2

In the context of the coronavirus pandemic, one of the priority tasks is the disinfection of environmental objects in order to comply with the anti-epidemic regime. In this regard, questions arise regarding the laboratory control of the effectiveness of disinfection measures. The use of virological approaches is hardly acceptable for solving this problem, and the use of fast and affordable methods of molecular genetic analysis comes to the fore, which are certainly of signal value, but sufficient to evaluate the effectiveness of these measures. In this work, studies were carried out on the detection of SARS-CoV-2 coronavirus RNA by several test systems using the real-time polymerase chain reaction method in swabs from various surfaces after they were treated with a disinfectant. The study showed that to control the decontamination of environmental objects potentially infected with the SARS-CoV-2 coronavirus, test systems can be used to detect the RNA of the SARS-CoV-2 virus in clinical samples. The greatest sensitivity is shown for PCR kits in which multichannel detection of individual RNA sites is carried out. Keywords: disinfectants, decontamination control, novel coronavirus infection COVID-19.

Prevention of pathologies of the hooves of cows in conditions of intensive milk production

The results of studying the effectiveness of the system for the prevention of orthopedic pathology in Holstein cattle, based on the maximum possible exclusion of technological stress in the processing of hooves, are reflected. During the orthopedic medical examination of 475 female cattle, 265 sick animals were identified and the spread of surgical diseases of the hooves was established. The most massively registered cases of Mortellaro disease (24.0%) and Rusterholtz ulcer (20.0%). Tyloma (3.2%), white line disease (3.2%), purulent pododermatitis (1.9%), etc. were much less common. The lack of planned disinfection measures, irregular and long intervals between hooves treatments artificially lengthening with the development of infertility of animals were essential in the etiology of these pathologies.&#x0D;

Bacterial Resistance Toward Antimicrobial Ionic Liquids Mediated by Multidrug Efflux Pumps.

The effective elimination of foodborne pathogens through cleaning and disinfection measures is of great importance to the food processing industry. As food producers rely heavily on disinfectants to control pathogenic bacteria in their facilities, the increasing spread of tolerant, often even multidrug resistant, strains is of particular concern. In addition to efforts to prevent or at least reduce development and spread of strains resistant to disinfectants and sanitizers, there is an urgent need for new and effective antimicrobials. One new class of promising antimicrobials is ionic liquids (ILs), which have been reported to be effective against resistant strains as they interact with bacterial cells in multiple ways, but investigations of their effectivity against MDR bacteria or specific defense mechanisms are still limited. This study investigates the role of multidrug efflux pumps of the Resistance Nodulation-Division family (RND) on the resistance of bacterial pathogens Escherichia coli and Salmonella enterica serovar Typhimurium toward 10 antimicrobial active ILs. Results reveal that, while known structure–activity relationships (SARs), such as the side-chain effect, were found for all strains, antimicrobial ILs with one elongated alkyl side chain were significantly affected by the RND efflux pump, highlighting the importance of efflux pumps for future IL toxicity studies. In case of antimicrobial ILs with multiple side chains and different cationic head groups, two ILs were identified that were highly active against all investigated strains with little to no effect of the efflux pump. The results obtained in this study for RND efflux pumps can serve as a starting point for identifying and designing antimicrobial ILs as effective biocides against MDR bacteria.

Infectious dose of Senecavirus A in market weight and neonatal pigs.

Foot-and-mouth disease virus (FMDV) is a picornavirus that produces a highly transmissible vesicular disease that can devastate meat and dairy production to such an extent that FMDV-free countries commit significant economic resources to maintain their FMDV-free status. Senecavirus A (SVA), also a picornavirus, causes vesicular disease in swine that is indistinguishable from FMDV. Since 2015, SVA outbreaks have been reported around the world requiring FMDV-free countries to investigate these cases to rule out FMDV. Understanding the pathogenesis of the SVA and its ability to transmit to naïve populations is critical to formulating control and prevention measures, which could reduce FMDV investigations. The primary objective of this study was to determine the infectious dose of SVA in market weight and neonatal pigs. A 2011 SVA isolate was serially hundred-fold diluted to create four challenge inoculums ranging from 106.5 to 100.5 TCID50/ml. Four market weight pigs individually housed were intranasally inoculated with 5 mL of each dose (n = 16). Serial ten-fold dilutions were used to create 6 challenge inoculums ranging from 105.5 to 100.5 TCID50/ml for neonatal pigs. Again, four animals in individual housing were challenged orally with 2 mL of each dose (n = 24). Detection of SVA by PCR in collected samples and/or neutralizing antibody response was utilized to classify an animal as infected. The minimum infectious dose for this study in market weight animals was 1,260 TCID50/ml (103.1 TCID50/ml) and for neonates it was 316 TCID50/ml (102.5 TCID50/ml). Knowledge of the infectious dose of SVA can guide biosecurity and disinfection measures to control the spread of SVA.

Population characteristics and source analysis of microorganisms in a regularly disinfected library

This study investigated the impact of regular disinfection on the concentration of microbial aerosol and the distribution of microbial species in a university library and analysed the main sources of microorganisms. The microorganisms in the air were sampled by six-stage Andersen cascade impactors, and their diversity was explored based on ribosomal DNA site amplification and sequencing technology. The statistical analysis found that the average concentrations of bacteria and fungi in this library were 149 ± 36 CFU/m3 and 118 ± 33 CFU/m3, respectively. The concentration in the reading rooms, the hybrid rooms and the stack rooms decreased in succession. The disinfection effect was significant comparing the result with non-disinfected libraries reported in literature. The concentration ratio of indoor and outdoor microorganisms (I/O) was greater than 1, and the concentration of microbial aerosol was positively correlated with the concentration of CO2 and particulate matter. The results indicate that the microbial concentration was mainly from indoor occupants. The most prevalent microorganisms were Paenibacillus, Bacillus, Penicillium and Cladosporium. This species' distribution was not affected by disinfection measures. The median diameter of microbial aerosols in most rooms was less than 5 μm. The use of disinfectants did not affect the particle size distribution of microorganisms.

Clinical and epidemiologic description of a severe outbreak of Salmonellosis in an urban nursery school

ObjetivosSe describe clínica y epidemiológicamente un brote de infección gastrointestinal por Salmonella entérica ser. (serotipo) Enteritidis, en una escuela infantil urbana, que conllevó elevada morbilidad e importante alarma social. La comunicación inmediata, así como el estudio adecuado del brote, en ambas vertientes, permitieron identificar el patógeno y establecer medidas de control en un plazo razonable de tiempo. Se discuten aspectos controvertidos como la indicación de antibioterapia o el momento de cierre del centro.Material y métodosSe recogió retrospectivamente información clínica, analítica y epidemiológica, y se revisó la metodología y resultados del estudio del brote.Resultados57 niños (3-45 meses) de 92 asistentes al centro, fueron afectados y tuvieron confirmación microbiológica. Diarrea y fiebre fueron los principales síntomas. 74% acudieron al hospital, y 37% ingresaron, (estancia media 3,3 días). Fueron factores asociados al ingreso: deshidratación, elevación significativa de reactantes de fase aguda y coagulopatía. 12 recibieron cefotaxima parenteral. Se registraron 2 complicaciones: 1 bacteriemia y 1 reingreso. La sospecha inicial del origen del brote fueron los alimentos, pero el análisis de las muestras testigo fue negativo. 5 trabajadores fueron positivos (2 sintomáticos). Vigilancia Epidemiológica concluyó que el origen probable del brote fue un portador asintomático y la manipulación incorrecta de pañales. El centro permaneció cerrado 8 días. Se realizaron medidas de limpieza y desinfección, instrucción sobre cambio de pañales y seguimiento de portadores.ConclusionesLa agrupación en tiempo y espacio de casos debe ser notificada inmediatamente para el control precoz del brote. Los niños pueden presentar formas graves de gastroenteritis por Salmonella.

EPIDEMIOLOGICAL SITUATION ON LAMBLIASIS IN ISFARA

The data of the Center for State Sanitary and Epidemiological Surveillance for Isfara were analyzed for 2016-2020. The data were analyzed for 1860 patients of different ages divided into four groups. The first group included children aged 0 to 4 years, the second group from 5 to 14, the third group from 15 to 17 and the fourth group from 18 and older. The presented data showed that the morbidity of children aged 5 to 14 years was higher than in the adult population. In rural areas, the incidence of lambliasis was 85.3%. Mostly children from 1 to 14 years old suffered from the disease, they made up 90.3% of the total number of registered patients with lambliasis during the analyzed period. Lambliasis infected mainly undisciplined children in jamoats of Surkh, Chorku and Vorukh. The infection rate in preschool institutions was 2.9% (54 cases). Adults made up 0.9% of all patients. The prevention of lambliasis among children involves observing the personal hygiene rules, timely detection and treatment of patients, disinfection measures in the foci, and intensified sanitary and educational work among the population.

Nosocomial Infection in SARS COV-2 Pandemic

In SARS COV-2 pandemic, the healthcare facilities are the most actively functioning setups which are the critical (microbiologically) areas. Due to these facilities patients were benefitted but during this time many health care providers suffered from nosocomial infection and many were complaining about the spread of nosocomial infection. Hospitals and healthcare facilities are easy place for contacting with infection, but the spread of infection should be stopped by taking proper disinfection measures.

Molecular Characterization of Carbapenem-Resistant Acinetobacter baumannii Isolates Among Intensive Care Unit Patients and Environment.

ObjectiveCritical patients in intensive care unit (ICU) are highly susceptible to acquiring carbapenem-resistant Acinetobacter baumannii (CRAB) infection. To investigate the relationship between nosocomial infections and environmental health, we studied the distribution and homology of CRAB isolates from patients and environment and evaluated the effectiveness of infection control measures.MethodsIn the 4-month study, we conducted a monthly CRAB screening of the ICU environment prior to disinfection in a Chinese teaching hospital. The ICU underwent routine disinfection procedures twice a day. We collected samples from the environment around the patients before disinfection. Clinical specimens from patients were also screened. The samples obtained were studied for phenotype and homology via antibiotic susceptibility testing, pulsed-field gel electrophoresis (PFGE), and whole-genome sequencing (WGS).ResultsTen specimens were sampled from ICU environments. Five were obtained in May 2020, and sputums from patient a in bed A at this time were cultured for CRAB isolates; in June 2020 another 5 environmental specimens were obtained from the same bed unit for CRAB, and sputums from patient b in bed A at this time were also cultured for CRAB isolates. Following intensive infection control measures, environmental sampling was negative in July and August. These 18 CRAB isolates all carried OXA-66 and OXA-23 genes and showed a similar resistance phenotype. WGS showed a close relationship among specimens from patients’ sputum and their surroundings, but no homology between patients.ConclusionThe analysis of cgMLST and SNPs is more accurate for strain homology analysis. Our data confirm that CRAB isolates spread from patient to environment in ICU; however, contact isolation and disinfection measures are effective in avoiding transmission, highlighting the importance of continued education and surveillance of CRAB. WGS could provide rich information on antimicrobial resistance, which is of great value in scientific research and clinical diagnosis.