Diseases In Traditional Medicine Research Articles

Phytochemical Contents, GC-MS Analysis and Hepatoprotective Effect of the Methanol Leaf Extract of Camelliasinensis (L.) Kuntze on Paracetamol-Induced Liver Injury in Wistar Rats

BACKGROUND AND OBJECTIVES: _Camellia sinensis_ is a tree popularly called green tea used as a strong antioxidant and for treating diseases such as cancer. This present study was aimed at assessing the phytochemicals and evaluating the hepatoprotective activity of leaf extract in Wistar rats. METHODS: The phytochemical screening was performed by standard methods, GC-MS analysis was carried out in Agilent 7890 A GC coupled to a MS detector, acute toxicity study was evaluated by the OECD guidelines, and hepatoprotective activity were evaluated in randomly groups of five rats of six rats in paracetamol-induced liver toxicity, while liver biomarkers were evaluated using automated methods. RESULTS: The phytochemical screening of leaf extract revealed the presence of major metabolites. The GC-MS analysis showed 18 bioactive compounds which are mainly fatty acids with retention times between 7.436 and 18.462 min. A high total phenolic content (TPC) of 1425.22 mg GAE/g and a total flavonoid content (TFC) of 802.01 mg QE/g were obtained. The acute toxicity did not produce any sign of toxicity at a dose of 5,000 mg/kg of extract. The extract produced a dose-dependent activity on the liver. These protections were statistically significant (p<0.05) when compared to the standard drug silymarin. There were no elevated values in liver function biomarkers in all the animal groups as well as histopathological damages to the liver after eight weeks of treatment at 200 and 400 mg/kg b.w. of extract doses. CONCLUSION: In conclusion, the study showed that _C. sinensis_ methanol leaf extract contains important phytochemicals that are protective against paracetamol-induced liver injuries, thus, the plant was considered safe for use as ethnomedicinal remedies for liver disease in traditional medicine.

The use of Phytochemical, GC-MS Analysis and Hepatoprotective Effect of the Methanol Leaf Extract of Camellia Sinensis (L.) Kuntze on Paracetamol-Induced Liver Injury in Wistar Rats

BACKGROUND AND OBJECTIVES: Camellia sinensis is a tree popularly called green tea used as a strong antioxidant and for treating diseases such as cancer. This present study was aimed at assessing the phytochemicals and evaluating the hepatoprotective activity of leaf extract in Wistar rats. METHODS: The phytochemical screening was performed by standard methods, acute toxicity study and hepatoprotective activity were evaluated in randomly groups of five rats or six rats in paracetamol-induced liver toxicity. RESULTS: The phytochemical screening of leaf extract revealed the presence of major metabolites. The GC-MS analysis showed 18 bioactive compounds which are mainly fatty acids with retention times between 7.436 and 18.462 min. A high total phenolic content (TPC) of 1425.22 mg GAE/g and a total flavonoid content (TFC) of 802.01 mg QE/g were obtained. The acute toxicity did not produce any sign of toxicity at a dose of 5,000 mg/kg of extract. The extract produced a dose-dependent activity on the liver. These protections were statistically significant (p<0.05) when compared to the standard drug silymarin. There were no elevated values in liver function biomarkers in all the animal groups as well as histopathological damages to the liver after eight weeks of treatment at 200 and 400 mg/kg b.w. of extract doses. CONCLUSION: In conclusion, the study showed that C. sinensis methanol leaf extract contains important phytochemicals that are protective against paracetamol-induced liver injuries, thus, the plant was considered safe for use as ethnomedicinal remedies for liver disease in traditional medicine.

ANTIMICROBIAL POTENCY AND PHYTOCHEMICAL CONTENTS OF THREE COMMON NIGERIA MEDICINAL PLANTS’ LEAF EXTRACTS

Morinda lucida, Tithonia diversifolia and Carica papaya are three medicinal plants commonly used for curing several diseases in traditional medicine. However, comparative scientific information as regards their antibacterial, antifungal, and phytochemical properties remains limited. Fresh leaves of Morinda lucida, Tithonia diversifolia, and Carica papaya were harvested and extracted by soaking pulverized samples of the plants in 250 mL of water and ethanol. The phytochemical constituents were determined by subjecting the decoction to qualitative and quantitative tests using standard methods. The antimicrobial and antifungal activities were determined by solidified Mueller-Hinton agar plate and solidified Potato Dextrose agar plate respectively. Alkaloids, flavonoids, saponins, tannins, and terpenoids were shown to be the primary metabolites in the ethanolic extract of the three plant species. Cardiac glycosides are only present in T. diversifolia. Aqueous and ethanolic leaf extracts of the three species had significant, but varying antibacterial activity and no antifungal properties. Ethanolic extracts of Morinda lucida were the most active against Escherichia coli with a 30 mm zone of inhibition and very active against Klebsiella pneumonia and Enterobacter aerogenes with 28 mm and 24 mm zone of inhibition, respectively. The aqueous extracts of T. diversifolia were more active against Staphylococcus aureus, Serratia marscences, and Enterobacter aerogenes than ethanolic extract. The ethanolic decoction of C. papaya was active against Enterococci. This study showed that depending on the decoction solvent and the microbes under investigation, the leaf extracts of the three plant species have a tolerable but variable level of activity.

Assessment of Protective Roles of Aqueous Seed Extract of Sour-Sop (Annona muricata) in Cerebellum Following Cadmium-induced Neurotoxicity of Adult Wistar Rats

Cadmium, a deleterious heavy metal that pervades the environment, has the potential to accumulate in the body and cause health complications. In the domain of traditional medicine, medicinal plants have been employed to combat the toxicity of heavy metals and treating of many diseases in traditional medicine.
 Aim: To access the protective impact of Annona muricata seed extract on cadmium-triggered neurotoxicity in the cerebellum of albino wistarrat.
 Place of Study: Department of Anatomy, Faculty of Basic Medical Sciences Olabisi Onabanjo University, Ogun State between June 2022 and June 2023
 METHODOLOGY: After a period of 14 days of acclimatization, 40 healthy male wistar rats were randomly allocated to four groups, group1(2ml/kg of distilled water), group2 cadmium only(2g/kg SC), group3 (2g/kg cadmium + 100mg/kg extract of Annona muricata) and group4(2g/kg cadmium + 200mg/kg extract of Annona muricata) The rats were subjected to cadmium subcutaneously followed by the oral administration of aqueous seed extract of Annona Muricata for fifteen consecutive days.
 Results: The impact of the extract on antioxidant enzymes activities of the cerebellum, cerebellar weight, as well as the histology of the cerebellum were scrutinized. The data revealed that the aqueous seed extract of Annona muricata, in a dose-dependent manner, augmented the levels of superoxide dismutase (SOD) (P=0.005) and catalase (CAT) (P=0.027) in contrast to the cadmium-only group. The relative weight of the cerebellum exhibited a significant increasein the treated groups compared to the cadmium-only group.
 The histology of the cerebellum delineated pathological changes arising from the exposure to cadmium, while Annona muricata brought about regenerative changes.
 Conclusion: In summary, the study posits that owing to the presence of phytochemicals in Annona muricata aqueous seed extract, it was efficacious in mitigating the neurotoxicity induced by cadmium in the cerebellum of male rats.

Effects of Usnic Acid on Cytotoxicity, Colony Formation and Migration in SK-UT-1 Human Uterine Leiomyosarcoma Cells

Usnic acid (UA) is a common lichenic secondary metabolite with therapeutic potential. Anticancer, antimicrobial, and antioxidant effects have been demonstrated and UA-enriched extracts are often used to treat various diseases in traditional medicine. First, we performed a viability assay of UA in human uterine leiomyosarcoma (SK-UT-1) since no in vitro cytotoxicity data have been reported so far. For this purpose, the cytotoxicity of UA on SK-UT-1 and embryonic kidney (HEK293) cells was studied at 24 and 48 hours. The toxicity of UA was investigated by the MTT test. In addition, we assessed UA colony formation and migration properties against SK-UT-1 cells in 6-well plates. Our results showed a significant cytotoxic effect of UA at the 5.34 µM; UA was also effective against colony formation ability and wound healing assay. In conclusion, our study suggests that UA exerts anti-proliferative effects on SK-UT-1 cells by inducing cell death. Moreover, our results suggest that the potential anticancer activities of UA should be further studied by additional molecular biological approaches.

Network pharmacology based virtual screening of Flavonoids from Dodonea angustifolia and the molecular mechanism against inflammation

Inflammation is a nonspecific immune response against injury caused by a harmful agent that strives to restore tissue function and homeostasis. Dodonaea angustifolia L.f. (Sapindaceae) is a medium-sized shrub used to treat a variety of diseases in traditional medicine. In the current study, integrated network-pharmacology and molecular docking approaches were used to identify the active constituents, their possible targets, signaling pathways, and anti-inflammatory effects of flavonoids from D.angustifolia. D. angustifolia active ingredients were acquired from the Indian Medicinal Plants, Phytochemistry and Therapeutics (IMPPAT), and Traditional Chinese Medicine System Pharmacology (TCMSP) databases. The screening included the ten most prevalent D. angustifolia components, and the SwissTargetPrediction database was utilized to anticipate the targets of these compounds. Anti-inflammatory genes were found using the GeneCards database. The 175 overlapping genes were discovered as prospective D. angustifolia anti-inflammatory targets. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that the overlapped targets were closely related to the major pathogenic processes linked to inflammation, such as response to organonitrogen compound, protein kinase activity, phosphotransferase activity, pI3k-Akt signaling pathway, metabolic pathways, and chemical carcinogenesis. Compound–target–pathway, and protein–protein interaction networks revealed 6-Methoxykaempferol and 5-Hydroxy-7,8 dimethoxyflavone as key compounds, and AKT1, VEGFA, and EGFR as key targets. Furthermore, molecular docking followed by molecular dynamic (MD) simulation of D. angustifolia active ingredients with core proteins fully complemented the binding affinity of these compounds and indicated stable complexes at the docked site. These findings reveal D. angustifolia 's multi-target, multi-compound, and multi-pathway strategies against inflammation. Our study paved the way for further research into the mechanism for developing D. angustifolia -based natural products as alternative therapies for inflammation.

Seasonal changes in fatty acid profile of the freshwater snail Viviparus contectus (Millet, 1813) from lake habitat

ABSTRACT The freshwater snail, Viviparus contectus, is an important shellfish owing to its nutritional composition (i.e., high amounts of protein, essential amino acids, fatty acid profile and minerals) and its promise as a candidate for the treatment of some diseases in traditional medicine. Hence, the seasonal changes in the fatty acid (FA) profile of Viviparus contectus caught from Demirköprü Dam Lake were determined in this study. Saturated fatty acid (SFAs) (64.54–80.39% of total fatty acids) had the highest concentration followed by polyunsaturated fatty acids (PUFAs) (10.63–21.83%) and monounsaturated fatty acids (MUFAs) (6.57–10.13%). Stearic acid (C18:0) was the major SFA while arachidonic acid (C20:4 n−6) was the major PUFA. The season significantly affected the FA profile of V. contectus while no significant difference was determined between seasons with respect to tissue MUFAs levels. A higher level of total SFAs was detected during spring (80.39%) while a higher proportion of MUFAs was detected during the winter (10.13%). The total amounts of PUFAs were highest in winter (21.83%). Overall, V. contectus has nutritional value. However, V. contectus caught in autumn may be nutritionally the most valuable for human consumption due to higher eicosapentaenoic acid 20:5 n−3 and docosahexaenoic acid contents.

Evaluation of the Antidiabetic Potentials of Musa acuminata Leaves Crude Extract in Alloxan-Induced Wistar Rats

Context: Musa acuminata is a plant of the tropical and subtropical regions. Over the past few decades, the health benefits of Musa acuminata have received much attention. All parts of the plant, including fruits, peel, pseudo stem, corm, flowers, leaves, sap, and roots, have found their use in treating many diseases in traditional medicine.
 Aim: This study was conducted to appraise the protective effect of Musa acuminata extract on alloxan-induced Diabetics Mellitus in wistar rats.
 Settings and Design: This investigation was carried out using 24 Wistar rats, both males and females. The experimental models were divided into six groups; 4 rats per group.
 Materials and Methods: The experimental models were divided into six groups; 4 rats per group. Alloxan was intraperitonially injected at a dose of 150 mg/kg body weight for day 1 across all groups except the positive control which is Group A together with oral administration of the aqueous extract of Musa acuminata (100 mg/kg, 200mg/kg and 300mg/kg body weight for the treatment group) and Group F was administered a standard drug. The animals were sacrificed on the 11th day under deep anesthesia with chloroform. The blood was collected by cardiac puncture and kidneys were collected for the histological profile.
 Statistical Analysis: Kidney oxidative stress marker Malondialdehyde (MDA) and antioxidant enzyme activities Creatinine and Urea, were determined.
 Results: The alloxan injection indicated an increase in Malondialdehyde (MDA), with a decrease in Urea and Creatinine when compared with the control. A remarkable decrease in antioxidant enzymes was also observed. Oxidant/antioxidant imbalance, alloxan-induced diabetic mellitus, and histological changes in the kidneys were reduced almost to normal by the administration of Musa acuminata.
 Conclusion: Based on the current findings, Musa acuminata leaves at low dose (100 mg/kg body weight) in the alloxan Induced Wistar rat has some anti diabetics potential than at 200 mg/kg body weight, 300 mg/kg body weight and standard drug (Metformin).

Flavonol Glycosides from Eugenia uniflora Leaves and Their In Vitro Cytotoxicity, Antioxidant and Anti-Inflammatory Activities

In view of the extensive use of Eugenia uniflora leaves for the management of tumours and other chronic inflammatory diseases in traditional medicine, an activity-guided fractionation of its leaf ethanolic extract led to the isolation of two flavonol glycosides. Cytotoxicity study was based on the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) viability assay against the non-tumourigenic human embryonic kidney (HEK-293) cells, and the cancerous liver (Hep-G2) and cervical (HeLa) cell lines. Antioxidant tests were carried out using 2,2-diphenyl-1-picrylhydrazyl (DPPH), nitric oxide (NO) and hydrogen peroxide (H2O2) radical scavenging assays, while an in vitro anti-inflammatory test was conducted using egg albumin denaturation (EAD) assay. Based on comprehensive spectroscopic and spectrometric evidence, the compounds were elucidated as myricitrin (1) and a newly described compound, 5,7-dihydroxy-3-(3,4,5-trihydroxy-6-methyltetrahydropyran-2-yloxy)-2-(2,4,5-trihydroxyphenyl)chromen-4-one, named “unifloratrin (2)”. The cytotoxicity of myricitrin (1) was comparable to 5-fluorouracil (standard drug), with a CC50 of 8.5 ± 2.2 µg/100 µL against HeLa cells. It also demonstrated better antioxidant activity, with an IC50 of 6.23 ± 1.09, 22.01 ± 2.59 and 30.46 ± 1.79 µM against DPPH, NO and H2O2 free radicals, respectively. At 20 µg/mL and an incubation time of 2 h, myricitrin was comparable to diclofenac (standard drug) in anti-inflammatory activity. This report may serve as a justification for the ethnomedicinal use of E. uniflora, while flavonol glycosides, such as myricitrin (1), could be further exploited as a candidate cytotoxic agent.

Breast cancer cell growth arrest and chemopreventive effects of Passiflora edulis Sims (Passifloraceae) ethanolic leaves extract on a rat model of mammary carcinoma.

Despite various prevention and treatment measures, the incidence and mortality due to breast cancer has been increasing globally. Passiflora edulis Sims is a plant used for the treatment of various diseases in traditional medicine, including cancers. To assess the anti-breast cancer activity of the ethanolic extract of P. edulis leaves in vitro and in vivo. In vitro, the cell growth and proliferation were determined based on the MTT and BrdU assays. The flow cytometry was used to analyze the cell death mechanism while, cell migration, cell adhesion and chemotaxis were assayed for anti-metastatic potential. In vivo, 56 female Wistar rats aged 45-50 days (∼75g) were exposed to 7,12-dimethylbenz(a)anthracene-DMBA except the normal group. Negative control group (DMBA) received solvent dilution throughout the study; standards groups (tamoxifen - 3.3mg/kg BW and letrozole - 1mg/kg BW) as well as P. edulis leaves ethanolic extract groups (50, 100 and 200mg/kg) treated for 20 weeks. Tumor incidence, tumor burden and volume, CA 15-3 serum' level, antioxidant, inflammatory status and histopathology were assessed. P. edulis extract showed a significant and concentration-dependent inhibition of MCF-7 and MDA-MB 231cells growth at 100μg/mL. It inhibited cell proliferation and clones' formation and induced apoptosis in MDA-MB 231cells. The migration of cell into the zone freed of cells and the number of invading cells after the 48 and 72h were significantly diminished while, it increased their adherence to collagen and fibronectin extracellular matrix as does Doxorubicin. In vivo, all rats in the DMBA group exhibited a significant (p < 0.001) increase in tumor volume, tumor burden and grade (adenocarcinoma of SBR III) and pro-inflammatory cytokine levels (TNF-α, INF-γ, IL-6 and IL-12). P. edulis extract at all tested doses significantly inhibited the DMBA-induced increase in tumor incidence, tumor burden and grade (SBR I) as well as pro-inflammatory cytokines. Moreover, it increased enzymatic and non-enzymatic antioxidants (SOD, catalase, and GSH) and decreased MDA levels although a greater effect was observed with Tamoxifen and Letrozole. P. edulis has medium content on polyphenols, flavonoids and tannins. P. edulis has chemo-preventive effects against DMBA-induced breast cancer in rats probably through its antioxidative, anti-inflammatory and apoptosis-inducing potentials.

Antioxidant and antithrombotic activities of the Distichochlamys citrea leaves extract

Distichochlamys citrea (DC) is an endemic ginger species used in treating associated-heart diseases in traditional medicine in Vietnam. However, scientific evidence to support the local use of this plant was limited. The present study aimed to investigate the antioxidant and antithrombotic activities of D. citrea extracts for the first time. The antioxidant activity of DC extracts was assessed by scavenging DPPH radical and measuring their total phenolic content (TPC). The antithrombotic activity was evaluated by inhibiting platelet aggregation and prolonging blood coagulation. Volatile components elucidated by GC-MS were docked with typical platelet receptors, including COX-1 and P2Y12. Results showed that the methanol extract of D. citrea exhibited a stronger DPPH scavenging ability (IC50 = 0.33 ± 0.00 mg/mL) and a higher TPC (8.09 ± 0.21%) than other extracts (p &lt; 0.05). On the other hand, the hexane extract of D. citrea (DC-HX) had a remarkable inhibiting impact on ADP-, collagen- and ristocetin–induced platelet aggregation in a dose-dependent manner (Pearson’s correlation, r &gt; 0.90, p &lt; 0.05). In contrast, this extract did not lengthen the clotting time through any factors, such as PT (prothrombin time), and TT (thrombin time), except for APTT (activated partial thromboplastin time) at 4 mg/mL of the extract. GC-MS revealed that oxygenated hydrocarbons (54.45%) dominated the volatile profile of DC-HX, followed by sesquiterpens (37.18%) and diterpenes (6.66%). In the platelet aggregation process, several compounds in DC-HX were firmly bound to COX-1 and P2Y12, which might partly explain the significant antiaggregatory activity of this fraction. In conclusion, Distichochlamys citrea may be a potential source of active phytoconstituents for treating radicals- and cardiovascular-associated diseases.

The Anti-Acne Potential and Chemical Composition of Knautia drymeia Heuff. and Knautia macedonica Griseb Extracts.

The treatment of acne and other seborrheic diseases has arisen as a significant clinical challenge due to the increasing appearance of multi-drug resistant pathogens and a high frequency of recurrent lesions. Taking into consideration the fact that some Knautia species are valuable curatives in skin diseases in traditional medicine, we assumed that the thus far unstudied species K. drymeia and K. macedonica may be a source of active substances used in skin diseases. The purpose of this study was to evaluate the antioxidant, anti-inflammatory, antibacterial, and cytotoxic activities of their extracts and fractions. An LC-MS analysis revealed the presence of 47 compounds belonging to flavonoids and phenolic acids in both species while the GC-MS procedure allowed for the identification mainly sugar derivatives, phytosterols, and fatty acids and their esters. The ethanol as well as methanol-acetone-water (3:1:1) extracts of K. drymeia (KDE and KDM) exhibited great ability to scavenge free radicals and good capacity to inhibit cyclooxygenase-1, cyclooxygenase-2, and lipoxygenase. Moreover, they had the most favorable low minimal inhibitory concentration values against acne strains, and importantly, they were non-toxic toward normal skin fibroblasts. In conclusion, K. drymeia extracts seem to be promising and safe agents for further biomedical applications.

Antioxidant, Cytotoxicity, Apoptotic Properties of Extracts of Andricus sternlichti galls and Their Phenolic Characterisation by HPLC.

Quercus L. galls have been used in Western and Eastern cultures for various diseases in traditional medicine. Galls are also used in the East for many purposes, including consumption as food, commercial inks, leather tanning. In the current study, Andricus sternlichti Bellido, Pujade-Villar & Melika, 2003 galls were extracted in different solvents. The possible antioxidant effects of gall extracts were determined using 7 different methods (β-carotene-linoleic acid assay, Phosphomolybdenum assay, DPPH and ABTS radical scavenging activity, CUPRAC and FRAP assay, Metal Chelating activity) to support each other. Total phenolic, flavonoid and tannin amounts of extracts are calculated by using standard curves. In addition, HPLC method used to characterize the phenolic component with 15 different standards. The MIA PaCa-2 cell lines was preferred to identify possible cytotoxic activities of galls. Expression of some genes (Bax, Bcl-2, FAS, BID, caspase-3, caspase-8, caspase-9, caspase-10, FADD, TRADD) role in the apoptosis was determined to investigate apoptotic effects of extracts. According the results, the gall extracts of A. sternlichti may be considered as a potential source of biological agents for their antioxidant capacity and rich bioactive compounds. The gall extracts exhibit antiproliferative activity via regulating expressions of apoptotic genes.

Plants Used in Digestive System Diseases in Traditional Medicine (Ardahan, Iğdır and Kars): Cross-Sectional Study

Plants Used in Digestive System Diseases in Traditional Medicine (Ardahan, Iğdır and Kars): Cross-Sectional Study

Contribution to the Study of The Antipyretic Effects of Aqueous Extracts of Two Plants « Urtica dioica and Nerium oleander ».

The current treatment of febrile conditions involves non-steroidal anti-inflammatory drugs (NSAIDs), which are widely prescribed because of their efficacy in the management of pain, fever, and inflammation. However, their long-term therapeutic use is often associated with adverse effects such as gastrointestinal ulcers. Therefore, current research is based on the substitution of chemical treatments with natural treatments that are equally effective but have fewer side effects. One of the most widespread remedies is the use of substances of plant origin. Urtica dioica and Nerium oleander are two medicinal plants widely used for the treatment of various diseases in traditional medicine. The aim of this study was to evaluate the antipyretic effect of the aqueous extract of these plants in vivo. The administration of these extracts significantly reduced brewer's yeast-induced hyperthermia. The results of this study contribute to validating the medicinal virtues of these plants, especially in the treatment of febrile states. The phytochemical constituents are probably responsible for their pharmacological properties and could replace some drugs as they cause undesirable side effects.

The Effect of Bioactive Compounds of Prunus spinosa Leaves and Flowers Mixture on the Antioxidant Capacity in Streptozotocin-induced Diabetic Rats

Diabetes is a serious and chronic public health problem worldwide. Hyperglycemia leads to the production of free radicals thus impairing oxidant/antioxidant balance and causing various complications in diabetes. Herbs can pro- vide natural remedies against various diseases in traditional medicine. Phyto- chemicals especially phenolic compounds have some biological properties such as antioxidant and antidiabetic effects. Plant parts of Prunus spinosa are used in traditional medicine against various disorders. This study evaluated the mixture of Prunus spinosa leaves and flowers extract (PSE) on the antioxidant capacity in diabetes. The phenolic profile of PSE was determined by HPLC method. Antioxidant capacity was evaluated with radical scavenging activities as well as total phenolic and flavonoid content. Seven random groups (n = 8) of rats were constituted to evaluate the antioxidant capacity – control, diabetic, PSE25, PSE50, insulin, metformin, and acarbose. Total oxidant status (TOS) and total antioxidant status (TAS) were analyzed in the liver tissues. Chlorogenic acid and ellagic acid were found to be the main compound in the mixture of P. spinosa leaves and flowers extract. Antioxidant capacity of PSE exhibited effective radical scavenging activity. Although oxidative stress seriously increased in diabetes, PSE25 and PSE50 supplementation significantly recuperated liver antioxidant status. In conclusion, PSE markedly has antioxidant capacity through its high phenolic compounds, thus PSE treatment may have potential antioxidant effects for health benefits in diabetes.

Evaluation of phenolic profiles, antioxidant activities, and cytotoxic and apoptotic potentials of Phlomis angustissima and Phlomis fruticosa, medicinal plants from Turkey

The genus Phlomis belongs to the family Lamiaceae and has over 100 species distributed across Europe, Asia, North Africa, and Turkey. Some Phlomis species are consumed as herbal teas for various diseases in traditional medicine. This study investigates the phenolic profiles, antioxidant activities, and cytotoxic properties of the methanol, ethyl acetate, and water extracts of leaves and flowers of P. angustissima, which is endemic to Turkey, and of P. fruticosa. Basic phenolics were identified by RP-HPLC-DAD. Antioxidant parameters and total- phenolics, -flavonoids, and -tannins were analysed spectrophotometrically. MTT cell proliferation was detected with a microplate reader. Apoptosis was analysed by flow cytometry. Among the identified phenolics, hesperidin, kaempferol, catechin, epicatechin, epigallocatechin, eupatorin, and chlorogenic, vanillic, syringic, p-coumaric, ferulic, and benzoic acids are reported in this study for the first time. The values of radical scavenging capacities (DPPH·, HO·, and NO·) and antioxidant activities (metal chelating and reducing power) were found to be significant. Both Phlomis species are rich in total-phenolics, -flavonoids, and -tannins. The best IC50 values of the P. angustissima and P. fruticosa extracts against five different cancer cell lines were, respectively determined as 23±1.42 and 26±1.68 ppm against ACC-201 cells, 40±2.32 and 28±2.1 ppm against OE-33 cells, and 80±3.21 ppm against HeLa cells (P. fruticosa), and the death types of the cancer cells were apoptotic. The results obtained for P. angustissima and P. fruticosa show that they are promising in terms of future applicability in food and medical fields as natural biopotential resources.

Therapeutic Uses and Prospects of Cyanthillium cinereum - The Underrated Herb

The whole plant of Cyanthillium cinereum has several pharmacological properties in treating a broad range of diseases in traditional medicine but underestimated to be used as a commercial drug. As a remedy to several health conditions, phytocompounds of C. cinereum can be used as multi-target drugs to treat comorbidity. This review elaborates on the therapeutic benefits of the herb, C. cinereum and its prospects in the treatment of diseases.

A Review of Antifungal Activities of Ziziphora clinopodioides

Ziziphora clinopodioides, also known with vernacular names such as Kakuti-e kuhi, Pinah kuhi, Ankh, Lip vanilla, and mountain mint, is a wild flowering plant that belongs to the Lamiaceae family. It is used in treating typhoid fever, stomach strengthening, abdominal pains, inflammatory and cardiovascular disease, asthma, cough, bronchitis, insomnia, colds, flu, and other infectious diseases in traditional medicine. Additionally, Z. clinopodioides has various biological activities, including antibacterial, antimicrobial, anti-inflammatory, antioxidant, appetizer, carminative, antiseptic, and wound-healing properties. In this study, the antifungal activities of Z. clinopodioides were summarized. The Keywords were searched in Scopus until 19 October 2021 and the articles that contain relevant information about the antifungal activity of Z. clinopodioides were included. Z. clinopodioides leaf and aerial parts had significant antifungal activity against Aspergillus flavus, Aspergillus parasiticus, Candida albicans, Candida glabrata, Candida guilliermondii, Candida krusei, Epidermophyton floccosum, Microsporum canis, Microsporum gypseum, Trichophyton mentagrophytes, Trichophyton rubrum, and Trichophyton schoenleini. In some studies, its effects were higher than standards (such as amphotericin B, fluconazole, nystatin, and terbinafine). Therefore, it seems that Z. clinopodioides can be a good choice for more experimental and clinical studies as an antifungal agent.

A Review on Treatment of Alzheimer's Disease in Traditional Medicine Vs. Modern Science

Alzheimer's is a degenerative brain disease associated with memory depletion and mild cognitive dysfunction, leading to dementia. Medicinal plants have a major role to play in Alzheimer’s and memory loss control. Ayurveda, homeopathy, Siddha and Unani medicine systems are main conventional therapeutic approaches. Herbal remedies, like that of the Egyptians, Chinese and Indians have their roots in ancient times. Use of medicinal herbs in of AD treatment includes improving overall health and well-being. Interest in medicinal herbs has grown fast during the recent years leading to increased interest of researchers in therapeutic utilization plants for treatment and enhancement of disease and wellbeing, often without severe side effects. The modern age of drug discovery in Alzheimer's disease began with the proposal of the cholinergic memory impairment hypothesis and the 1984 research criteria for AD. There are modest symptom-reducing effects of FDA authorized medications, but they really does not affect the eventual course of the AD. In the campaign against Alzheimer's disease, the hunt for newer drugs which are safe and effective are already required the cooperation of large number of researchers with many willing subjects. But that's a long way off! 146 medications have been rejected since 1998. Popular herbal plants for treating Alzheimer's disease are discussed in this review. The report also centered on FDA-approved medications for treating Alzheimer's disease. This review discusses all aspects of AD treatment including the comparison between the traditional and the Allopathic medicines that was missed by some articles. Most review studies focus on either traditional treatment or the management in Modern science of the disease but rarely on a comparison of both. The study also addresses the possibilities for the management of AD in future.