In the presented research, the dynamic working of nickel-based (IN600) superalloy was performed and the micro-mechanisms affecting the deformation behaviour were closely monitored, particularly the role of static and dynamic recrystallization. Hot deformation flow stress response indicated an increase in the true stress value with an increase in strain rate and decrease in temperature. The constitutive calculations indicated that deformation progressed mainly due to dislocation climb motion. The hot workability map indicated a peak power dissipation efficiency (PDE) of 0.39 in two working domains. The microstructural evolution in the hot deformed specimens depicted a consistent increase in dynamic softening at temperature ≥ 1000 °C, whereas, a steady decline in the extent of dynamic softening is observed with increase in strain rate from 0.01 s−1 to 1 s−1. The mechanism for microstructural evolution in this range (0.01–1 s−1) was identified as discontinuous dynamic recrystallization (DDRX). Moreover, at the highest strain rate of 10 s−1, a sudden surge in the recrystallization fraction was observed, which was attributed to the combined effect of DDRX and static recrystallization (SRX). Additionally, this study shows that the presence of twin boundaries (TBs) contributes to dynamic softening by serving as sites for nucleation of strain-free grains.