It has been known that groundwater-lake water interaction plays an important role in mass exchange between land and lakes. However, most of the lakes in the world freeze during the winter, and few studies of groundwater and surface water interaction (groundwater discharge tracing) focus on ice-covered lakes. This study investigated groundwater discharge into a large ice-covered lake, Xiao Qaidam Lake in the Tibetan Plateau (TP), using satellite remote sensing and chemical and isotopic tracing. Analysis of chemical components, stable isotopes (D, 18O) and 222Rn revealed that groundwater discharge occurred in the western and southern portions of Xiao Qaidam Lake. Remote sensing data (the distribution of and variation in lake surface temperature, LST) revealed these locations of groundwater discharge and indicated additional groundwater discharge in areas of springs. Based on the regression model between LST and 222Rn activity in lake water and 222Rn mass balance model, the groundwater discharge into Xiao Qaidam Lake was estimated to be (2.77 ± 0.26) × 105 m3/d, which is slightly higher than river water input at summer. In addition, this study also suggests that ice melting process should be considered when tracing groundwater discharge into an ice-covered lake, and that the LST distribution during the icing period is better than the LST variation for tracing groundwater discharge because the ice eliminated noise from wind and surface water. The study provides insight into the tracing of groundwater discharge into large ice-covered lakes in the TP and elsewhere.