A simple field method for measuring relative net productivity in flowing waters is described. To assess its sensitivity and practicality, the eutrophication potential of a tertiary sewage treatment plant effluent was assayed on the Fort River of Amherst, Massachusetts. Two sets of three 10 m lexan tubes (1.25 cm i.d.) were anchored to the river bed 100 m upstream and downstream from the sewage plant outfall. Data collection began following a 2 week colonization period for the periphyton community. Substrate primary productivity (mg O 2 min −1) was determined by subtracting inflow dissolved oxygen concentrations from these levels at the discharge ends of each tube and multiplying the difference by the flow rate. The downstream tubes demonstrated an enhanced response at a 0.01 level of significance to an increment of 0.005 and 0.1 ppm of phosphorous and nitrogen respectively. Data analysis revealed a highly significant regression of the net productivity on stream flow which was adjusted for by an analysis of covariance. The productivity of the downstream station was distinctly higher (0.4522 mg O 2 min −1) then the value of the upstream station (0.2584 mg O 2 min −1). The protocol thus affords a biological summation of the sewage effluent through comparison with the control station replicates.
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