CAN controllers are responsible for the processing and transmission of CAN signals, which render them indispensable electronic devices in automotive cyber-physical systems. SJA1000T is one of the most common CAN communication chips which can be implemented through the use of bulk silicon CMOS technology. A susceptibility test platform is built based on the direct power injection method following IEC62132-4 to explore the electromagnetic susceptibility characteristics and the effects of thermal stress on CAN controller. Test results show that the susceptibility threshold curve is corresponded directly to frequency and temperature. The influence of thermal stress on the input and output characteristics of MOSFET is simulated and analyzed to verify the anti-interference ability and reduced reliability of the chip in a high temperature environment.