Recent progress of transmission electron microscope(TEM) enables us to perform electron diffraction from nm-sized areas(nano-diffraction) with a good correspondence with HREM. Nano-diffraction is useful for obtaining local structural information. One of the present authors (NT) applied the method to interfaces of GaAs/AlGaAs superlattices for detection of compositional variation of aluminum(Al) and to nm-sized γ-iron crystallites in MgO for analysis of the strain. Although nano-diffraction has been performed successfully in STEM instruments by Cowley and others, the method in TEM has an advantage in a good compatibility with the structure-imaging method and the established bright and dark-field(BF & DF) imaging methods. In the present paper we report for the first time the detection of lattice strain around interfaces of InP/InGaP strained superlattices as well as results of GaAs/InGaAs, GaAs/AlGaAs and Ge/Si superlattices by using the TEM nano-diffraction method.The nano-diffraction was performed with 200 kV transmission electron microscopes of Cs = 1.2 mm(JEM-2000FX) and Cs = 0.5mm(JEM-2010L Before focusing an election probe, HREM and DF images of the interfaces were taken in the [100] zone axis at 100-400 k in direct magnification.