Direct Approach Research Articles

Discrimination among American Indian and Alaska Native people: implications for public health communication.

American Indian and Alaska Native People (AI/AN) have experienced discrimination stemming from sustained attempts to erase AI/AN People and their culture or livelihood. Research identifying the types of discrimination experienced by AI/AN People is needed to help individuals recognize discrimination in daily life. We examine experiences of discrimination among an urban AI/AN population using a mixed methods approach. Self-identified AI/AN participants (N = 303, n = 294 with complete data; 63% women, mean age = 43 years) were recruited from the Denver-metro area in Colorado. Stress and coping models of discrimination guided our analysis. Exposure to discrimination was quantitatively assessed via the Brief Perceived Ethnic Questionnaire - Community Version (BPEDQ-CV), a self-report measure including four subscales assessing workplace discrimination, social exclusion, physical threat and harassment, and stigmatization. Participants responded to a laboratory recall task in which they described an episode of discrimination and their affective and coping responses. Content analysis was conducted on transcribed responses to illustrate discrimination exposure as reported in the BPEDQ-CV and in prior theoretical work on coping with discrimination. Repeated measures analyses revealed participants reported experiencing social exclusion more than other forms of discrimination, followed by reports of workplace discrimination, stigmatization, and physical threat. Consistent with these quantitative findings, participants were more likely to recall experiences of social threat (94%), including episodes of workplace discrimination, social exclusion, and stigmatization than physical threat and harassment. Almost half the participants (47%) reported confronting or directly addressing the discrimination, and 38% reported avoiding a direct approach. For 44% of participants, their predominant emotional response included internalizing emotions such as fear/sadness/embarrassment, and another 44% reported experiencing externalizing emotions, including anger. Our descriptive findings present the experiences of urban AI/AN People who have experienced many forms of unjust and prejudicial treatment. These data can provide useful information to help the general public and AI/AN individuals more readily recognize and prevent discriminatory behavior, and consequently mitigate deleterious effects of discrimination on health.

Antibody levels versus vaccination status in the outcome of older adults with COVID-19.

BACKGROUNDDespite the currently prevailing, milder Omicron variant of COVID-19, older adults remain at elevated risk of hospital admission, critical illness, and death. Loss of efficacy of the immune system, including reduced strength, quality, and durability of antibody responses, may render generalized recommendations on booster vaccinations inadequate. There is a lack of data on the efficacy of antibody levels in older adults and on the utility of vaccination status versus antibody levels as a correlate of protection. It is further unclear whether antibody levels may be used to guide the timing of booster vaccinations in older adults.METHODSWe conducted a prospective multicenter cohort study comprising hospitalized patients with COVID-19. Anti-SARS-CoV-2 spike antibodies were measured on hospital admission. The primary endpoint was in-hospital mortality. Patients were stratified by age, antibody levels, and vaccination status. Multiple logistic regression and Cox regression analyses were conducted.RESULTSIn total, 785 older patients (≥60 years of age [a]) and 367 controls (<60a) were included. After adjusting for confounders, risk of mortality, ICU admission, endotracheal intubation, and oxygen administration was 4.9, 2.6, 6.5, and 2.3 times higher, respectively, if antibody levels were < 1,200 BAU/mL (aOR, 4.92 [95%CI, 2.59-9.34], P < 0.0001; aOR, 2.64 [95%CI, 1.52-4.62], P = 0.0006; aOR, 6.50 [95%CI, 1.48-28.47], P = 0.013; aOR, 2.34 [95%CI, 1.60-3.343], P < 0.0001). Older adults infected with the Omicron variant were approximately 6 times more likely to die if antibody levels were < 1,200 BAU/mL (aOR, 6.3 [95% CI, 2.43-16.40], P = 0.0002).CONCLUSIONAntibody levels were a stronger predictor of in-hospital mortality than vaccination status. Monitoring antibody levels may constitute a better and more direct approach for safeguarding older adults from adverse COVID-19 outcomes.

A Novel Time-Domain Direct Sampling Approach for Inverse Scattering Problems in Acoustics

A Novel Time-Domain Direct Sampling Approach for Inverse Scattering Problems in Acoustics

Gender Differences in Request Strategies among Graduate Students

Proficiency in request strategies is key in handling social hierarchies and managing interpersonal communication. It is also essential in reducing misunderstandings and enhancing communication efficiency in both educational and professional settings. This quantitative survey investigated the request strategies employed by graduate students in different hierarchical contexts. The sample population consisted of 100 B.Ed. (Bachelor of Education) major English students from ten campuses affiliated to Tribhuvan University (TU). The sample was selected employing stratified simple random sampling method. The data were collected using a structured questionnaire with nine closed-ended questions in the year 2081 BS. The data were analyzed using descriptive statistics technique of quantitative data analysis. The result revealed distinct patterns in request strategies when addressing individuals of varying ranks. In higher-to-lower rank scenarios, male respondents predominantly used mood derivable strategies, while females showed a preference for performatives and hedge performatives. For equal-to-equal rank situations, males favored direct mood derivable strategies, while females used a more varied approach with higher incidences of indirect strategies. In lower-to-higher rank contexts, males again showed a preference for direct approaches, whereas females exhibited a higher use of indirect and polite strategies. These findings highlight significant gender-based differences in communication strategies which emphasize the need for personalized approaches in educational and professional settings.

Rapid recovery after total hip arthroplasty: direct anterior approach combined with PENG block and lateral cutaneous femoral nerve block

Introduction The "gold" standard for the treatment of late stages of coxarthrosis is total hip arthroplasty. Direct anterior approach (DAA) refers to minimally invasive surgical interventions in orthopaedics. Extended anesthetic measures in combination with low-traumatic surgical techniques may reduce postoperative pain and accelerate patient's recovery.The purpose of the study was to compare the recovery times of patients after hip arthroplasty using DAA in combination with PENG block, lateral cutaneous femoral nerve (LCFN) block and without extended anesthetic measures.Materials and methods A prospective randomized comparative clinical study was performed, which involved 62 patients divided into two groups: the study one (n = 29) and the control one (n = 33). In both groups, arthroplasty was performed using DAA. Patients of the study group underwent PENG block and LCFN block. The patients in the control group did not receive extended anesthesia. The evaluation criteria were pain assessment using the visual analogue scale (VAS), administration of painkillers, patient’s mobility and the length of hospital stay.Results The VAS score for pain in the study group were lower than in the control group after 6 hours — 3.7 (3.4; 4.1) and 4.3 (4.2; 4.8); 24 hours after surgery — 3.5 (3.3; 3.6) and 4.1 (3.9; 4.5) (p &lt; 0.001). After 48 hours, the indices were comparable: 3.5 (3.1; 4.1) and 3.7 (3.6; 3.9) (p = 0.19). The rate of requests for pain relief in the first 24 hours was lower in the study group than in the control group: 2 (1; 2) and 3 (2; 3) cases (p = 0.003). The results of the manual muscle test after 6 hours and 24 hours were comparable (p &gt; 0.05). The time interval between the end of the operation and the first walking on crutches was shorter in the study group — 3.1 hours (2.9; 3.4) and 3.98 hours (3.8; 4.2) (p &lt; 0.001). The length of hospital stay was shorter in the study group: 1.5 (1.2; 2) and 2.5 (2; 3) days (p &lt; 0.001).Discussion Lower postoperative pain allows faster activation of patients, thus improving the results of the early rehabilitation period.Conclusion The use of PENG block and LCFN block in arthroplasty with the use of DAA has clinical effectiveness in the first 24 hours, and helps to accelerate the postoperative recovery of patients.

Comparison of early complications for primary total hip arthroplasty using modified direct anterior approach and lateral approach

Comparison of early complications for primary total hip arthroplasty using modified direct anterior approach and lateral approach

Mapping RANKL- and OPG-expressing cells in bone tissue: the bone surface cells as activators of osteoclastogenesis and promoters of the denosumab rebound effect.

Denosumab is a monoclonal anti-RANKL antibody that inhibits bone resorption, increases bone mass, and reduces fracture risk. Denosumab discontinuation causes an extensive wave of rebound resorption, but the cellular mechanisms remain poorly characterized. We utilized in situ hybridization (ISH) as a direct approach to identify the cells that activate osteoclastogenesis through the RANKL/OPG pathway. ISH was performed across species, skeletal sites, and following recombinant OPG (OPG:Fc) and parathyroid hormone 1-34 (PTH) treatment of mice. OPG:Fc treatment in mice induced an increased expression of RANKL mRNA mainly in trabecular, but not endocortical bone surface cells. Additionally, a decreased expression of OPG mRNA was detected in bone surface cells and osteocytes of both compartments. A similar but more pronounced effect on RANKL and OPG expression was seen one hour after PTH treatment. These findings suggest that bone surface cells and osteocytes conjointly regulate the activation of osteoclastogenesis, and that OPG:Fc treatment induces a local accumulation of osteoclastogenic activation sites, ready to recruit and activate osteoclasts upon treatment discontinuation. Analysis of publicly available single-cell RNA sequencing (scRNAseq) data from murine bone marrow stromal cells revealed that Tnfsf11+ cells expressed high levels of Mmp13, Limch1, and Wif1, confirming their osteoprogenitor status. ISH confirmed co-expression of Mmp13 and Tnfsf11 in bone surface cells of both vehicle- and OPG:Fc-treated mice. Under physiological conditions of human/mouse bone, RANKL is expressed mainly by osteoprogenitors proximate to the osteoclasts, while OPG is expressed mainly by osteocytes and bone-forming osteoblasts.

EXPERIENCES OF FILIPINO TEACHERS IN THE GULF COOPERATION COUNCIL (GCC) COUNTRIES’ HIGHER EDUCATION INSTITUTIONS

This study explores the experiences of Filipino teachers working in higher education institutions within Gulf Cooperation Council (GCC) countries, focusing on the challenges they encounter and the teaching strategies they apply. In-depth interviews were done by researchers using qualitative methodologies. Criterion sampling is used to choose the participants for this study. The criteria are as follows: teachers should be teaching in a Gulf Cooperation Council (GCC) country; (2) they should have rendered at least 9-11 years of teaching experience to capture a wide range of perspectives in higher educational institutions in the GCC countries; and (3) Participants should be from the undergraduate programme. The researchers interviewed five participants from higher education institutions in one of the GCC countries for IDI, all of whom fell within Creswell's recommended ranges. The collected data was subjected to a thematic analysis while ethical guidelines were followed. The study results highlight that Filipino teachers in GCC higher education institutions face challenges and opportunities in adapting to the teaching and learning process. This includes the utilization of the spoon-feeding method, the direct teaching approach, and the application of the Socratic method. Teachers also reported dealing with minimal student engagement, having difficulty managing students, being passive students, having poor attendance, and having a language barrier. The study underscores the importance of tailored support systems to enhance the integration and effectiveness of foreign educators in GCC higher education. These insights contribute to a deeper understanding of the global mobility of educators and the evolving landscape of higher education in the GCC region.&lt;p&gt; &lt;/p&gt;&lt;p&gt;&lt;strong&gt; Article visualizations:&lt;/strong&gt;&lt;/p&gt;&lt;p&gt;&lt;img src="/-counters-/soc/0713/a.php" alt="Hit counter" /&gt;&lt;/p&gt;

Exploring an adaptive management model for “status-optimization-regulation” of mining city in transition: A case study of Huangshi, China

Mining cities face the challenges of ecological transformation and sustainable development after mineral depletion. Thus, ecological space optimization and adaptive management are pivotal after ecological restoration project, but easily neglected. Taking Huangshi in Hubei Province as an example, a top-down adaptive management model for regional mining ecological space integrating status-optimization-regulation (SOR) was established based on land system resilience (LSR) evaluation, circuit theory, complex network model and community discovery algorithm. The results showed that i) As the LSR increased, the land use structure shifted from non-ecological zones to forest and cropland, indicating that balancing agriculture and forest protection was crucial for increasing LSR in Huangshi. i) The ecological networks (ENs) of Huangshi had an irregular fishnet pattern, with densely intricate corridors in the south and broader, sparser ones in the north, and numerous ecological barriers near the mining area. Furthermore, the distribution of ecological sources and corridors displayed complementarity and hierarchy. iii) The optimized ENs exhibited higher connectivity and efficiency under disturbance, reducing corridor redundancy and migration costs. iv) The ENs clusters were classified into five types based on ecological connectivity: ecological buffer zone, priority restoration zone, moderate restoration zone, natural restoration zone, and moderate development zone, and tailored ecological regulatory strategies were proposed. The findings provide practice-oriented guidance for the sustainable development of mining cities, and offer a direct approach to support conscious, clear, and coherent adaptive management of ecological restoration in rapidly changing environments.

Does the utilization of fluoroscopy affect the accuracy of prosthesis position in patients undergoing hip replacement surgery via the direct anterior approach compared to the posterolateral approach for an experienced surgeon? A single-center retrospective study

BackgroundThe direct anterior approach (DAA) for total hip arthroplasty (THA) has gained popularity due to perceived advantages in achieving better early outcomes. However, the theoretical benefits such as precise implant positioning and accurate leg length restoration are still inconsistent. In this study, we compared implant position and leg length discrepancy (LLD) of the DAA and posterolateral approach (PLA) conducted by a single surgeon who had mastered both approaches.MethodsThis study retrospectively reviewed the medical records and radiographic images of 244 patients who underwent THA between 2012 and 2021 by a single experienced surgeon using either the DAA (n = 120) or PLA (n = 124). Postoperative pelvic anteroposterior radiographs at 6 months follow-up were used to assess acetabular component inclination and anteversion, as well as LLD. Mann-Whitney U tests and Chi-squared test were performed to compare outcomes between the two approaches.ResultsNo significant differences were observed in patient demographics between the two groups. The DAA group demonstrated significantly lower postoperative LLD (0.00 mm) compared to the PLA group (5.00 mm, p < 0.0001). No significant difference was observed in the Lewinnek zone for cup anteversion and inclination angles between the two groups.ConclusionsFor experienced surgeons in other approaches, our findings suggest transferring to the DAA may not substantially improve cup positioning, but it might slightly enhance limb length measurement. For surgeons already proficient in other approaches and deciding to maintain those approaches, their primary concern for optimal THA outcome should be striving for leg length equivalence.

Thermal Error Transfer Prediction Modeling of Machine Tool Spindle with Self-Attention Mechanism-Based Feature Fusion

Thermal errors affect machining accuracy in high-speed precision machining. The variability of machine tool operating conditions poses a challenge to the modeling of thermal errors. In this paper, a thermal error model based on transfer temperature feature fusion is proposed. Firstly, the temperature information fusion features are built as inputs to the model, which is based on a self-attention mechanism to assign weights to the temperature information and fuse the features. Secondly, an improved direct normalization-based adaptive matrix approach is proposed, updating the background matrix using an autoencoder and reconstructing the adaptive matrix to realize domain self-adaptation. In addition, for the improved adaptive matrix, a criterion for determining whether the working conditions are transferrable to each other is proposed. The proposed method shows high prediction accuracy while ensuring training efficiency. Finally, thermal error experiments are performed on a VCM850 CNC machine tool.

Cooking Energy, Health, and Happiness of Women in Nigeria

This study utilizes novel data to investigate the impact of cooking energy sources and indoor air pollution on the happiness, life satisfaction, physical, and mental health of women in Nigeria. The existing body of literature relies on ambient air pollution data, which can be limiting in resource-constrained settings. To address this gap, we employ a direct approach, measuring Carbon Monoxide (CO) levels in participants’ blood using the Rad-57 CO-oximeter. Our analysis reveals strong positive correlations between the utilization of clean cooking energy and women’s reported happiness and life satisfaction. Additionally, the study finds that clean cooking energy usage is associated with a significant reduction in mental health problems among women. These findings highlight a substantial disparity in well-being based on access to clean cooking energy sources. Furthermore, exposure to carbon monoxide, as measured in this study, demonstrates a detrimental effect on women’s health and overall well-being. Consequently, policymakers and stakeholders should prioritize initiatives that promote household energy access and facilitate the transition to clean cooking practices, especially in rural areas where the use of polluting fuels and exposure to indoor air pollution remain prevalent concerns. JEL Classification: D12, I31, I100, Q420.

Quasi-Diabatization Based on Minimizing Derivative Couplings in a Limited Configuration Space: Elimination of Boundary Condition Dependence.

Due to the cuspidal ridges of adiabatic potential energy surfaces (PESs) and singularities of nonadiabatic couplings (NACs), obtaining an analytical expression for the adiabatic Hamiltonian is difficult. Thereby, nonadiabatic dynamics simulations are often carried out on-the-fly, which is time-consuming. This motivates us to construct quasi-diabatic representations, which have smooth PESs and diabatic couplings. In this study, we propose a new quasi-diabatization method based on minimizing derivative couplings (MDC) in a limited configuration space. The boundary conditions are first considered and finally released to obtain the adiabatic-to-diabatic rotation angles and transformation matrices. As demonstrated in representative one- and two-dimensional models and the widely studied linear H3 molecule, MDC performs significantly better than the direct integration quasi-diabatization approach. In particular, accurate diabatic potential energy matrices have been successfully obtained even when the NACs of all configurations in the considered space are nonnegligible.

A direct approach for solving the cubic Szegö equation

AbstractWe study the cubic Szegö equation which is an integrable nonlinear nondispersive and nonlocal evolution equation. In particular, we present a direct approach for obtaining the multiphase and multisoliton solutions as well as a special class of periodic solutions. Our method is substantially different from the existing one which relies mainly on the spectral analysis of the Hankel operator. We show that the cubic Szegö equation can be bilinearized through appropriate dependent variable transformations and then the solutions satisfy a set of bilinear equations. The proof is carried out within the framework of an elementary theory of determinants. Furthermore, we demonstrate that the eigenfunctions associated with the multiphase solutions satisfy the Lax pair for the cubic Szegö equation, providing an alternative proof of the solutions. Last, the eigenvalue problem for a periodic solution is solved exactly to obtain the analytical expressions of the eigenvalues.

Exploring nanopore direct sequencing performance of forensic STRs, SNPs, InDels, and DNA methylation markers in a single assay

IntroductionThe field of forensic DNA analysis has undergone rapid advancements in recent decades. The integration of massively parallel sequencing (MPS) has notably expanded the forensic toolkit, moving beyond identity matching to predicting phenotypic traits and biogeographical ancestry. This shift is of particular significance in cases where conventional DNA profiling fails to identify a single suspect. Supplementing forensic analyses with estimated biological age may be valuable but involves a complex and time-consuming DNA methylation analysis. This study explores and validates the performance of a comprehensive forensic third-generation sequencing assay utilizing Oxford Nanopore Technologies (ONT) in an adaptive and direct sequencing approach. We incorporated the most widely used forensic markers, i.e., STRs, SNPs, InDels, mitochondrial DNA (mtDNA), and two methylation-based clock classifiers, thereby combining forensic genetic and epigenetic analysis in one single workflow. Methods and resultsIn our investigation, DNA from six anonymous individuals was sequenced using the ONT standard adaptive direct sequencing approach, reaching a mean percentage of on-target reads ranging from 6.6 % to 7.7 % per sample. ONT data was compared to standard MPS data and Illumina EPIC DNA methylation profiles. Basecalling employed recommended ONT software packages. TREAT was used for ONT-based analysis of autosomal and Y-chromosome STRs, achieving 90–92 % correct calls depending on allelic read depth thresholds. InDel analyses for two lower-quality samples proved challenging due to inadequate read depth, while the remaining four samples significantly contributed to the observed percentage markers (60.9 %) and correct calls (97.8 %). SNP analysis achieved a 98 % call rate, with only two mismatches and two missed alleles. ONT-generated DNA methylation data demonstrated Pearson’s correlation coefficients with EPIC data ranging from 0.67 to 0.97 for Horvath’s clock. Additional age-associated markers exhibited Pearson’s correlation coefficients with chronological age between 0.14 (ELOVL2) and 0.96 (FHL2) at read depths of <30 and <20, respectively. Despite excluding mtDNA from our targeted sequencing approach, adaptive proof-reading fragments covered the complete mtDNA with an average read depth of 21–72, showing 100 % concordance with reference data. DiscussionOur exploratory study using ONT adaptive sequencing for conventional forensic and age associated DNA methylation markers showed high sequencing accuracy for a significant number of markers, showcasing ONT as a promising (epi)genetic forensic method. Future studies must address three critical aspects: determining clear quantity and quality measures and detection thresholds for accuracy, optimizing input DNA quantity for forensic casework expectations, and addressing ethical considerations associated with phenotype and ancestry analysis to prevent ethnic biases.

Direct approach on projective synchronization of inertial quaternion-valued neural networks with proportional delays

Direct approach on projective synchronization of inertial quaternion-valued neural networks with proportional delays

Survival Outcomes in US Medicare Patients with Non-Cystic Fibrosis Bronchiectasis by Rate of Baseline Exacerbations.

There are limited real-world data on outcomes in patients with non-cystic fibrosis bronchiectasis (NCFBE). This study assessed clinical characteristics and survival in patients with NCFBE by baseline exacerbation rate. Patients with bronchiectasis (≥ 1 inpatient or ≥ 2 outpatient claims with a bronchiectasis diagnosis code, or one outpatient claim with bronchiectasis code and a chest computed tomography scan) were from the 100% Medicare Fee-for-Service database (Jan 2014-Dec 2020). Patients had continuous enrollment ≥ 12months pre-index (baseline) and post-index (follow-up), with index a random bronchiectasis claim preceded by ≥ 12months bronchiectasis history. Patients with cystic fibrosis were excluded. Patients were stratified by exacerbations during baseline (0, 1, or ≥ 2). Follow-up exacerbation rate and all-cause mortality were assessed. Controls were identified using a multistep direct matching approach. Time to death from index was estimated by Kaplan-Meier analyses. Exacerbation analysis included 92,529 patients with NCFBE and 92,529 matched controls. Exacerbations were common (43% had ≥ 1 exacerbation), with patients with more baseline exacerbations more likely to have ≥ 2 exacerbations during follow-up (11.4%, 24.2%, and 46.8% of patients with 0, 1, and ≥ 2 baseline exacerbations, respectively). Survival analysis included 110,298 patients with NCFBE and 110,298 controls. Time to death was shorter in patients with more baseline exacerbations (P < 0.0001). Five-year survival was 55.3%, 62.6%, and 65.4% for patients with ≥ 2, 1, and 0 baseline exacerbations, respectively, compared with 64.1% for controls. In these patients with NCFBE, exacerbations were common. History of exacerbations was associated with future exacerbations and increased all-cause mortality.

Repeated interrogations of sources of human intelligence using the Scharff technique

ABSTRACT Human Intelligence (HUMINT) sources may be questioned multiple times. However, criminal and military interrogation research focuses on single-instance interrogations. The current study employed a role-playing paradigm to examine the effect of interrogation approach on various elicitation-relevant outcomes and information gain across repeated questioning sessions using a direct approach and the Scharff Technique. In the study scenario, participants (N = 68) were given information on an extremist group planning a bombing, were given an information management dilemma, and were subsequently questioned. Participants in the Scharff Technique condition were questioned using the Scharff Technique at Time 1 and direct questioning at Time 2. Direct Approach condition participants received direct questioning at both times. All participants provided a greater information contribution at Time 2 compared to Time 1, regardless of assigned condition. Compared to participants in the Direct Approach condition, participants in the Scharff Technique condition perceived Time 1 interrogators as more knowledgeable and Time 2 interrogators as less knowledgeable. This suggests a backfire effect when switching from the Scharff technique to direct questioning. Interrogators should carefully consider the decision to employ the Scharff technique if it may be followed up with more traditional questioning approaches.

Synthesis of 2,3-Diperfluoroalkylated Quinoxalines via Selenium-Catalyzed Reductive C-C Coupling of Vicinal Perfluoroalkyl Formimidoyl Chlorides.

A direct and efficient approach to access structurally interesting 2,3-diperfluoroalkylated quinoxalines via selenium-catalyzed reductive C-C construction of vicinal bis(perfluoroalkyl formimidoyl chloride)s has been disclosed. This protocol features the use of easily accessible starting materials, scalability, and a diverse functional group tolerance. Mechanism studies suggested that this reaction may involve an interesting selenium-containing seven-membered-ring intermediate and proceed through an electrocyclization/selenium reductive elimination pathway, which is significantly different from the traditional transition-metal-catalyzed reductive coupling strategies of alkyl halides.

Green and Sensitive Analysis of the Antihistaminic Drug Pheniramine Maleate and Its Main Toxic Impurity Using UPLC and TLC Methods, Blueness Assessment, and Greenness Assessments

For the first time, two direct and eco-friendly chromatographic approaches were adapted for the simultaneous estimation of pheniramine maleate (PAM) and its major toxic impurity, 2-benzyl pyridine (BNZ). Method A used reversed-phase ultra-performance liquid chromatography; separation was achieved within 4 min using a C18 column with a developing system of methanol/water (60:40 v/v) with a 0.1 mL/min flow rate. Photodiode array detection was adjusted at 215 nm. The method was linear in the ranges of 5.0–70.0 and 0.05–10.0 µg/mL for PAM and BNZ, correspondingly. Method B used thin-layer chromatography; separation was applied on silica gel TLC F254 using ethanol/ethyl acetate/liquid ammonia (8:2:0.1, in volumes) at room temperature, at 265 nm. Linearity was assured at concentration ranges 0.5–8.0 and 0.1–3.0 µg/band for the two components, respectively. Generally, the new UPLC and TLC methods outperform the old ones in terms of quickness, greenness, and sensitivity. Concisely, the greenness features were partially achieved using the Green Analytical Procedure Index (GAPI) and the Analytical Greenness (AGREE) pictograms. In contrast, the usefulness of the novel approaches was assured via the Blue Applicability Grade Index (BAGI) tool.