DIN EN Research Articles

The effect of different additives on bacteria adsorption, compressive strength and ammonia removal for MICP

Abstract Microbial induced calcite precipitation is a commonly used technique for the application of biocementation. The metabolism of urea by ureolytic active bacteria leads, among other metabolic products such as ammonium, to carbonate ions. In the presence of calcium ions, calcium carbonate formation occurs. It was investigated whether the addition of different additives (Ca-bentonite, Na-bentonite, clinoptilolite, natrolite, limestone, marl clay, concresol, secursol, and activated carbon powder) can optimize this process. First, the influence of these additives on the adsorption rates and distribution of the ureolytic active organism Sporosarcina pasteurii in quartz sand columns was tested. Moreover, an investigation was conducted on the impact of various additives on ammonium immobilization to mitigate its leaching from soils subjected to the biocementation process. For eight additives uniaxial compressive strength tests in quartz sand columns and a storage method according to DIN EN 12390–2 were carried out. Each additive showed a characteristic adsorption and localisation progression. Furthermore, each additive was able to enhance the immobilization behavior of the present ammonium, with a maximum immobilization capacity of 10.76 $$\frac{mg (NH^+_4)}{g (additive)}$$ m g ( N H 4 + ) g ( a d d i t i v e ) . The uniaxial compressive strength of biocemented columns out of quartz sand could sectionally be increased by the addition of each additive. However, the storage methodology shows a much greater influence on column strength. Overall, the best results were achieved with the two additives, Ca-bentonite and clinoptilolite, resulting in strength increases in sand columns of up to 4.64 and 3.22 $$\frac{N}{mm^2}$$ N m m 2 , respectively.

Façade eluates affect active and total soil microbiome

The application of biocides in building materials has become a prevalent practice to mitigate the growth of microorganisms such as algae, fungi, and bacteria on the façades. These can leach out from the material and reach the nearby soil environment. This study aimed to characterize the effect of façade eluates generated within different leaching experiments on total and metabolic active soil microbial community composition and functions. Façade eluates were produced by immersion testing DIN EN 16105 and a natural weathering experiment. Afterward, soil microcosms were treated with the respective façade eluate and incubated for 29 days. Subsequently, the active and total soil microbial community compositions were investigated.Fungal internal transcribed spacer region gene and bacterial 16S rRNA gene were sequenced for active (bromodeoxyuridine labeled DNA) microbial community and total community. Façade eluates reduced total bacterial and fungal gene copy numbers. Overall, active bacterial and fungal richness was reduced and altered in community composition in comparison to the total richness and composition, respectively. Façade eluates retrieved of façade samples without biocides did alter the soil microbial communities to the same extent as façade eluates with biocides. Additionally, members of the active microbiome that benefit from the presence of façade eluates and omitted ones could be identified. Our result demonstrated that façade eluates affect active and total soil microbial community composition and function regardless of the leaching procedure and biocides addition.

Adaptive slicing in powder bed fusion of metals using a laser beam—Investigation on productivity, laser absorption, geometrical accuracy, and thermal conditions

Powder bed fusion of metals using a laser beam (PBF-LB/M acc. to DIN EN ISO/ASTM 52900) has reached market maturity. In addition to developing new materials and enabling new applications, the industry focuses on increasing productivity and reducing costs. In this context, increasing the layer thickness can increase productivity but often leads to a deterioration of surface quality and part density. Using variable layer thicknesses depending on the manufactured geometry is well-known from filament-based material extrusion processes and is called adaptive slicing. This study investigates the manufacturing aspects of adaptive slicing using AlSi10Mg. Laser beam reflectance of the different powder layer thicknesses is quantified using diffuse reflectance infrared Fourier transform spectroscopy. The process window is identified and analyzed, focusing on the achievable productivity and required energy input. Furthermore, the suitability of layer thicknesses and processing parameters is analyzed by measuring the dimensional accuracy and process stability of overhanging structures. Heat input, heat dissipation, and potential heat-up are investigated and compared to conventional processes using part-scale thermal simulations. In this study, parameters are developed for layer thicknesses of 120 μm with an almost threefold increase in productivity in nonoverhanging structures with a part density above 99.7%. Further, adaptive slicing can increase productivity in PBF-LB/M while decreasing the impact on part quality. Future work will focus on automated algorithms to optimize and automize adaptive slicing.

Has the German vehicle fleet become quieter? - Results of a measurement campaign using SPB and CPX measurements

In the past, the German Environment Agency has carried out several measurement campaigns to determine the noise behavior of the German vehicle fleet. The aim of the current project was to identify any trends in comparison to previous campaigns. Autonomous pass-by measurements were carried out at 30 locations throughout Germany in accordance with DIN EN ISO 11819-1 (SPB) with the aim of recording at least 1000 vehicles per location. As the road surface influences the pass-by noise, near-field measurements according to DIN EN ISO 11819-2 (CPX) were carried out to determine the homogeneity of the road surface at each measurement location. In parallel to the CPX measurements, texture measurements according to DIN EN ISO 13473 were carried out to quantify the relevant geometric parameters of the road surface. The presentation describes the methodology of these measurements and presents the final results including a comparison with data from the 2012 and 2002 measurement campaigns.

Determination of the load acting on the probe by separating force and torque during FSW of AA 6060 T66

AbstractThe development of suitable welding processes is required to meet the ever-increasing demands on joining processes, particularly for lightweight construction and increasing environmental awareness. Friction stir welding (FSW) represents a promising alternative to conventional fusion welding processes, particularly for the joining of low-melting-point materials such as aluminium and magnesium alloys, which present a number of challenges, including the formation of pores and the occurrence of hot cracks. The central element of the process is the friction stir welding tool, which consists of a shoulder and a probe. The rotation and the simultaneous application of pressure during the joining process create a friction-based heat input through the tool. The excellent mechanical properties resulting from dynamic recrystallisation during the welding process are a major advantage of the process. As a result, strengths comparable to those of the base material can be achieved. However, FSW is subject to process-specific challenges, including high process forces, which result in the fabrication of complex and robust devices. Additionally, high dynamic loads on the friction stir welding tools must be considered. In many cases, the design of friction stir welding tools is based on empirical data. However, these empirical values are machine-, component- and material-specific, which often results in under- or overmatching of friction stir welding tools. Sudden probe failure, component scrap, and low process reliability are the direct consequences of undermatching. Overmatching results in enlarged tools with limited accessibility, high heat input, and high process forces, leading to component deformation. The aim of this study is to determine the load on the probe by separating the forces and torque of the shoulder and the probe in order to be able to make statements about the load acting on the probe and the resulting stress state. The knowledge of the stress state can be employed to design friction stir welding tools, both statically and dynamically, for a specific welding task. A strategy was devised to distribute the load exerted on the shoulder and probe. To this end, the length of the probe was gradually reduced between the welding tests. The investigations were carried out with a force-controlled robotized welding setup in which AA 6060 T66 sheets with a thickness of 5 mm were welded. A Kistler multicomponent dynamometer type 9139AA allows to measure the Cartesian forces to be recorded in the x-, y-, and z-directions with a sampling rate of 80 kHz. The weld seam properties were determined by visual and metallographic inspections as well as tensile and bending tests in accordance with DIN EN ISO 25239–5.

Safety barriers under changing operating conditions–An analysis of impact parameters in Germany

Vehicle restraint systems and especially the impact of a collision on such are subject to various influencing variables. These systems are tested and approved through vehicle impact tests to ensure that safety standards are met as well as to ensure comparability amongst the systems. However, differences in the objectives of the standard DIN EN 1317 and the behaviour in practice have become apparent.There is the standard DIN EN 1317 which defines the impact tests on testing grounds. Nevertheless, there are a number of influencing parameters such as developments in the vehicle fleet over the last years, available safety barriers, including their construction types and the impact itself. Previous research indicates that there could be a gap between these parameters.This research uses an empirical analysis based on chiefly quantitative data sources to evaluate the differences between these parameters. It leads to a partial divergence between static requirements of the standard and the actual road traffic conditions. Additionaly, the differences are developed within a vulnerability analysis and for the purpose of comparison, the advantages as well as the disadvantages of the standardized impact tests are discussed in this paper.As a result, a parameter based suggestion for a reevaluation of impact tests for safety barriers, according to the standard DIN EN 1317, is advisable due to the changing road traffic on the current stock and new barrier systems to be built. This research strives to illuminate the trend for new investigation methods such as the finite element method (FEM) simulation. It gives an outlook to further research needs in safety barriers, principally in the observation of the future development of the impact parameters. At the same time, impulses and potential for improvement can be identified for the future documentation of vehicle impacts on these barriers.

Self-ignition behaviour of corn cob, wheat bran and rice husk residues in ambient air from biomass gasification

The possibility of different agrowastes to self-ignite under ambient condition, due to exothermic reactions between their surface molecules and air or other oxidizing agents which are conveyed into the void volume between the particles, exists. It is imperative to investigate the self-ignition ability of these harzadous waste products causing environmental pollution after the milling process to avoid sudden fire outbreaks. In this study, the self-ignition attributes of corn cob, wheat bran and rice husk residues in ambient air from biomass gasification was investigated by evaluating their self-ignition temperatures using DIN EN 15188:2021 standard and Frank-Kamenetzkii's theory of thermal explosion at varying basket volume. The results revealed decrease in the ignition temperature of dust samples as ignition time and dust basket volume were increased. Sample C (rice husk dust residue) was considered to be the most hazardous with respect to its propensity to self-heating possessing the lowest self-ignition temperature of 173 °C at 800 mL cubic mesh. Its moisture content and activation energy of 1.41 % and 46.52 kJ/mol respectively were the lowest. Its thermal conductivity, carbon content, heating value and bulk density of 0.07 W/mK, 78.98 wt%, 26,895 kJ/kg and 255.4 kg/m3 respectively were the highest. Correlation coefficient from the Arrhenius plot showing the self-ignition behaviour of dust samples using the model of Frank-Kamenetzkii were 0.9976, 0.9910 and 0.9962 for corn cob, wheat bran and rice husk residues respectively. In conclusion, the data presented are effective in predicting the self-ignition ability of corn cob, wheat bran and rice husk residues in ambient air from biomass gasification in order to prevent sudden fire attack that may arise based on storage of their dust particles in food processing industries.

Growth, persistence and toxin production of pathogenic bacteria in plant-based drinking milk alternatives.

The present study investigated the microbiological safety of the increasingly popular plant-based milk alternatives. No (10/27) or only very low microbial counts (17/27) were detected in the tested products. These were mainly identified as spore formers via MALDI-ToF-MS. Three products contained Bacillus cereus group isolates, which were able to form considerable amounts of enterotoxins and exhibited cytotoxicity towards CaCo-2 cells. Preliminary tests showed good growth of B. cereus, Listeria monocytogenes, and Salmonella enterica in all tested products (maximum bacterial counts: 5×1012 cfu/mL). These experiments also revealed strain-, time-, and temperature-, but especially product-specific enterotoxin production of B. cereus. In propagation and persistence tests according to DIN EN ISO 20976-1:2019-09, rapid bacterial proliferation was also detected in all products. B. cereus generally showed lower bacterial counts (106-107 cfu/mL) compared to L. monocytogenes and S. enterica (108-109 cfu/mL), but was detectable in a larger number of products over the test period of 6 weeks. pH values decreased (20/27) over time and visual and/or olfactory alterations (24/27) were observed. The present study provides information on the occurrence, growth and persistence of pathogenic bacteria in plant-based drinking milk alternatives. It also points out that the accompanying changes in pH, odor, and appearance are not necessarily recognizable to the consumer. PRACTICAL APPLICATION: The present study contributes to the understanding of the microbial risk related to plant-based drinking milk alternatives. It is crucial that the manufacturer ensures that particularly spore formers have been effectively eliminated from the products. Among them, especially toxin-producing bacteria can pose a risk to the consumer, as these products promote proliferation and persistence of the bacteria.

Cement pastes containing air entraining agent release the biocide octylisothiazolinone posing ecotoxicological effects

We investigated whether cement pastes are a possible source of ecotoxicologically potent substances. For this purpose, leaching according to DIN EN 16637-2 was performed on portland cement pastes as well as blast furnace slag cement with and without an air entraining agent (AEA). The AEA, consisting of wood rosin and resin, contained the stabiliser drometrizole and the biocide octylisothiazolinone (OIT), which was confirmed by our non-target screening (NTS). Our ecotoxicological studies (Daphnia magna, Aliivibrio fischeri and Desmodesmus subspicatus) of the pure cement eluates showed no effects at all. In these samples, it was possible to attribute up to 85 % of the dissolved organic carbon (DOC) to acetate, formate and diethylene glycol (DiEG). Eluates from cement pastes with AEA contained up to 70 μg/L octylisothiazolinone (OIT), and no drometrizole was found. Around 90 % of the total OIT release happened within the first 6 h. It was possible to attribute the observed ecotoxicological effects mainly to the OIT concentrations. Additional leaching with elevated sulphate concentrations (800 mg/L) did not influence the release of DOC and OIT or increase the ecotoxicological effects. As a consequence, we advise curing the cement paste for 24 h prior to use, as this largely avoids the release of OIT and the observed ecotoxicological effects.

Enhancing Human-Computer Interaction in Socially Inclusive Contexts: Flow Heuristics and AI Systems in Compliance with DIN EN ISO 9241 Standards

In the domain of generative Artificial Intelligence (AI), the deployment of user-centered Human-Computer Interaction (HCI) methodologies, compliant with the DIN EN ISO 9241 standard, furnishes a substantive methodological scaffold. These methodologies have demonstrably succeeded in accomplishing specific, contextual objectives across a spectrum of applications, affirming their efficacy and user satisfaction. The investigation leverages a systematic theoretical framework and investigator triangulation to explore the customization of flow heuristics with respect to social permeability, inclusion, and accessibility. This approach facilitates the assimilation of multi-dimensional perspectives, thereby augmenting both accessibility and the user experience within these delineated domains. Within the ambit of enhancing HCI in socially inclusive settings, current heuristic models aimed at simplifying complexity in AI systems manifest notable shortcomings, particularly in relation to social permeability, inclusion, and accessibility. Academic scrutiny not only highlights these deficiencies in the application of DIN EN ISO 9241-210 standards but also uncovers substantial lacunae vis-à-vis the intricate demands of DIN EN ISO 9241-110. These encompass facets such as task appropriateness, self-descriptiveness, expectation conformity, error tolerance, controllability, individualizability, and learning facilitation, whose integrated and considered implementation is indispensable for the efficacious tailoring of interactive systems to meet specific user requirements in inclusive settings. This study significantly bolsters the epistemological base essential for the formulation of a conceptual framework tailored for AI deployment in educational and social management contexts, in alignment with DIN EN ISO 9241 standards. The study aims to articulate a detailed practical guide that outlines the requisite mechanisms, metrics, and characteristics necessary for user-centric adaptations in AI-enhanced systems. The objective is to augment the efficacy of flow induction and elevate user satisfaction within realms characterized by social permeability, inclusion, and accessibility.

Biphasic bone substitutes coated with PLGA incorporating therapeutic ions Sr2+ and Mg2+: cytotoxicity cascade and in vivo response of immune and bone regeneration.

The incorporation of bioactive ions into biomaterials has gained significant attention as a strategy to enhance bone tissue regeneration on the molecular level. However, little knowledge exists about the effects of the addition of these ions on the immune response and especially on the most important cellular regulators, the macrophages. Thus, this study aimed to investigate the in vitro cytocompatibility and in vivo regulation of bone remodeling and material-related immune responses of a biphasic bone substitute (BBS) coated with metal ions (Sr2+/Mg2+) and PLGA, using the pure BBS as control group. Initially, two cytocompatible modified material variants were identified according to the in vitro results obtained following the DIN EN ISO 10993-5 protocol. The surface structure and ion release of both materials were characterized using SEM-EDX and ICP-OES. The materials were then implanted into Wistar rats for 10, 30, and 90days using a cranial defect model. Histopathological and histomorphometrical analyses were applied to evaluate material degradation, bone regeneration, osteoconductivity, and immune response. The findings revealed that in all study groups comparable new bone formation were found. However, during the early implantation period, the BBS_Sr2+ group exhibited significantly faster regeneration compared to the other two groups. Additionally, all materials induced comparable tissue and immune responses involving high numbers of both pro-inflammatory macrophages and multinucleated giant cells (MNGCs). In conclusion, this study delved into the repercussions of therapeutic ion doping on bone regeneration patterns and inflammatory responses, offering insights for the advancement of a new generation of biphasic calcium phosphate materials with potential clinical applicability.

Photovoltaic, wireless wide‐field epiretinal prosthesis to treat retinitis pigmentosa

AbstractPurposeTo develop and evaluate a photovoltaic, wireless wide‐field epiretinal prosthesis for the treatment of retinitis pigmentosa.MethodsA mosaic array of thinned silicon‐based photodiodes with integrated thin‐film stimulation electrodes was fabricated with a flexible polyimide substrate film to form a film‐based miniaturized electronic system with wireless optical power and signal transmission and integrated electrostimulation. Manufactured implants were characterized with respect to their optoelectronic performance and biocompatibility following DIN EN ISO 10993.ResultsA 14 mm diameter prosthesis containing 1276 pixels with a maximum sensitivity at a near infrared wavelength of 905 nm and maximized stimulation current density 30–50 μm below the electrodes was developed for direct activation of retinal ganglion cells during epiretinal stimulation. Fabricated prostheses demonstrated mucosal tolerance and the preservation of both metabolic activity, proliferation and membrane integrity of human fibroblasts as well as the retinal functions of bovine retinas. Illumination of the prosthesis, which was placed epiretinally on an isolated perfused bovine retina, with infrared light resulted in electrophysiological recordings reminiscent of an a‐wave (hyperpolarization) and b‐wave (depolarization).ConclusionsA photovoltaic, wireless wide‐field epiretinal prosthesis for the treatment of retinitis pigmentosa using near infrared light for signal transmission was designed, manufactured and its biocompatibility and functionality demonstrated in vitro and ex vivo.

Development of a robust welding process for electron beam welding of thick plates for construction of offshore wind turbines

AbstractDue to its high energy intensity and the associated high welding depth, electron beam welding is particularly suitable for welding steel plates with high wall thicknesses. These are used, for example, in offshore wind power systems. However, due to the cooling conditions, the required cold toughness for offshore applications is often not achieved. The aim is to develop S355 ML steels – within the EN10025 4 standard – for welding monopiles for wind turbines. For this purpose, three parameters with different energy input are developed on three steels. The tests are carried out on plates with a wall thickness of 80 mm, whereby a full penetration weld must be achieved. The resulting top and bottom bead of the welds meet the standard DIN EN ISO 13919‐1. The parameters have an energy per unit length of 9.5 kJ mm−1 to 15.5 kJ mm−1. The resulting weld seams have an average width of 5.5 mm to 7.5 mm, and burn‐off of the manganese alloy element is observed, particularly on the top side of the seam. In addition, T8/5 times close to the weld seam of 11 s to 27 s are estimated using a simulation.

Quantitative Measurement and Evaluation of High-Resolution Ultrasonic Sound Fields using a Novel Automated Ultrasonic Immersion Scanner

Ultrasonic probes are an integral part of the automated ultrasonic non-destructive testing and evaluation (NDT&E) systems to detect and size the defects in a wide range of structural materials in production lines. A complete quantitative assessment of the performance characteristics of an application-specific UT probe is needed to be evaluated on the basis of the international standard DIN EN ISO 22232-2. This requirement of full assessment not only improves the quality assurance of the manufactured probes by evaluating the acceptance criteria but also provides the useful technical information to the end-user to optimize the automated ultrasonic testing on-site. In addition, the evaluation of probe characteristics should be carried periodically throughout their service life. In this work, we developed a novel high-precision ultrasonic immersion scanner to evaluate the full ultrasonic probe characteristics which include the squint angle measurement in three different reference planes (XY, XZ, and YZ), RF-signal and its frequency spectrum at water-steel interface, focal distance, focal range, focal width, focal length and also the angle of beam divergence in different planes of the sound field distribution. In the newly developed UT immersion scanner, for the first time, we successfully integrated the high-precision Hexapod with six axes to achieve a few micrometer (~15 µm) spatial-resolution in the measured sound field patterns and a good angular resolution (~0.05°) in the squint angle measurement. In addition, we used a verified UT instrument in accordance with the standard DIN EN ISO 22232-1 to obtain more accurate amplitude values (±0.5 dB) while scanning a test block. The automated scanning, data acquisition, evaluation, visualization and the automated test report generation are carried based on the standard DIN EN ISO 22232-2. The measured sound beam characteristics of both focused and non-focused application-specific ultrasonic probes with center frequencies ranging from 0.2 - 15 MHz using the pulse-echo technique on a 3 mm half-ball steel reflector are presented. In addition, the measured sound beams of an application-specific 5 MHz multi-element transmit-receive (TR) probe using the 3 mm flat-bottomed-hole (FBH) reflector in a steel reference plate are discussed. Finally, the quantitative analysis of the measured sound field results and the acceptance criteria in accordance with DIN EN ISO 22232-2 are discussed.

Increasing Energy Efficiency by Optimizing Heat Treatment Parameters for High-Alloyed Tool Steels

AbstractIn the paper industry, machine circular knives are used in the cutting process to provide industrial quality cuts on a variety of products. In the production of paper rolls, they cut the long-rolled paper products into commercial sizes. For this application, the high-alloyed ledeburitic cold work tool steel, DIN EN 1.2379 (X153CrMoV12; AISI D2), in the secondary hardened heat-treated condition has become the widely used industry solution. However, its heat treatment is a very energy-intensive production process. It consists of a quenching from an austenitizing temperature above 1050 °C, followed by three high-temperature tempering steps of more than 500 °C. In the study, the heat treatment process was optimized for energy efficiency, resulting in superior material properties with lower energy consumption. The most promising low energy heat treatment developed in the laboratory was reproduced in the industrial scale, and the required energy consumption was quantified. Subsequently, the resulting properties of the tools such as hardness, wear resistance and fracture toughness were determined. The energy production costs and mechanical properties of the tool steel were evaluated in comparison to conventional production methods. The newly applied heat treatment condition showed very promising and positive results in all analyzed parameters.

Influence of sodium hydroxide, silane, and siloxane treatments on the moisture sensitivity and mechanical properties of flax fiber composites

AbstractNatural fibers are a sustainable alternative to synthetic fibers due to their high weight‐specific Young's moduli and strengths. However, the mechanical properties of natural fibers are very sensitive to their moisture content. Therefore, chemical treatments are often applied to natural fibers to lower their water absorption and enhance fiber‐matrix interaction. The aim is to study the effects of fiber modifications with sodium hydroxide, silane, and siloxane on the water uptake and tensile properties of flax fiber composites produced via prepreg technology. In addition, the effect of moisture on the composites' tensile properties was investigated by conditioning one part of the tensile specimens according to DIN EN 2823 (at 70°C and 85% relative humidity). The NaOH treatment was the only modification that had positive effects on the Young's modulus and tensile strength in the unconditioned and conditioned state. The increase of the tensile modulus and strength are most likely due to changes in flax fiber composition, crystallinity of the cellulose and the rougher fiber surface of NaOH modified fibers. This shows that chemical treatment of natural fibers may improve the performance level of natural fiber composites and prevent a loss in their mechanical properties in humid environments.Highlights Flax fiber modifications with sodium hydroxide, silane, and siloxane. Flax fiber composite production via prepreg technology. Water uptake after conditioning at 70°C and 85% relative humidity. Tensile tests before and after conditioning. SEM images of modified flax fibers.

Influence of additives and binder on the physical properties of dental silicate glass-ceramic feedstock for additive manufacturing

ObjectivesThe aim of the study was to investigate the impact of organic additives (binder, plasticizer, and the cross-linking ink) in the formulation of water-based feedstocks on the properties of a dental feldspathic glass-ceramic material developed for the slurry-based additive manufacturing technology “LSD-print.” Material and methodsThree water-based feldspathic feedstocks were produced to study the effects of polyvinyl alcohol (AC1) and poly (sodium 4-styrenesulfonate) (AC2) as binder systems. A feedstock without organic additives was tested as the control group (CG). Disc-shaped (n = 15) and bar (n = 7) specimens were slip-cast and characterized in the green and fired states. In the green state, density and flexural strength were measured. In the fired state, density, shrinkage, flexural strength (FS), Weibull modulus, fracture toughness (KIC), Martens parameters, and microstructure were analyzed. Disc-shaped and bar specimens were also cut from commercially available CAD/CAM blocks and used as a target reference (TR) for the fired state. ResultsIn the green state, CG showed the highest bulk density but the lowest FS, while the highest FS in the green state was achieved with the addition of a cross-linking ink. After firing, no significant differences in density and a similar microstructure were observed for all slip-cast groups, indicating that almost complete densification could be achieved. The CAD/CAM specimens showed the highest mean FS, Weibull modulus, and KIC, with significant differences between some of the slip-cast groups. SignificanceThese results suggest that the investigated feedstocks are promising candidates for the slurry-based additive manufacturing of restorations meeting the class 1a requirements according to DIN EN ISO 6871:2019–01.

Influence of backing layers on the interlaminar fracture toughness energy – Mode I – of quasi-unidirectional GFRP

Abstract Quasi-unidirectional glass fiber non-crimp fabrics consist of a unidirectional main layer in the 0° direction stabilized by a backing layer in the 90° direction with a significantly lower amount of fibers. It is known that the backing layers impair the fatigue performance of respective reinforced plastics due to their orientation perpendicular to the main load direction. They act as damage initiators for the failure of the load-carrying unidirectional fibers. In the present work, the positive influence of the backing layers on the interlaminar fracture toughness energy – Mode I – G IC is demonstrated by tests in accordance with DIN EN 6033. The presence of backing fibers in the delamination plane can improve the G IC values by up to 43 %. Furthermore, results from evaluation according to DIN EN 6033 and ASTM D5528 are shown to have a good agreement, if the correction factor for large displacements recommended in the ASTM standard is not applied. If it is applied, however, there is a clear gap of up to 19 % between the two standards because the DIN simply does not provide a correction for large deflections.

Multiresidue analysis of bat guano using GC-MS/MS

Bats are the second largest mammalian order and are an endangered species group with a strong need for contamination monitoring. To facilitate non-invasive monitoring of the ecological burden in bat populations, a multiresidue method for the simultaneous quantification of 119 analytes including pesticides, persistent organic pollutants (POPs), active pharmaceutical ingredients (APIs), polycyclic aromatic hydrocarbons (PAHs), UV blockers, plasticizers, and other emerging pollutants in bat guano with gas chromatography tandem mass spectrometry (GC-MS/MS) was developed. Sample preparation and clean-up were performed with a modified QuEChERS approach based on DIN EN 15662. The method uses 1.00 g bat guano as sample with acetonitrile and water for liquid-liquid extraction. Phase separation is assisted by citrate-buffered salting out agent. For clean-up of the extract, primary secondary amine (PSA) was combined with graphitized carbon black (GCB). The lower limits of quantification (LLOQ) ranged between 2.5 and 250 µg kg−1. Linearity was shown in a concentration range from the respective LLOQs to 1250 µg kg−1. The median of the mean recovery was 102.4%. Precision was tested at three concentrations. Method and injection precision were adequate with a relative standard deviation (RSD) below 20%. Furthermore, the comparative analysis with LC-MS/MS demonstrated the reliability of the results and provided a valuable extension of the analytical scope. As proof of concept, three guano samples from a German nursery roost of Myotis myotis were analysed. The results show a time-dependent change in contaminant concentration, highlighting the strong need for non-invasive contamination monitoring of whole bat populations.Graphical

A spray-on environmentally friendly degradable mulch material and its high efficiency in controlling above-ground biomass of weeds in greenhouse experiments

AbstractThis study describes a novel spray-on mulch material as an alternative to currently used weed control methods. The mulch material is based on renewable raw materials, mainly rapeseed oil, starch and sodium alginate.Laboratory tests were conducted to obtain a mulch material with the best possible properties. Formulations with different ingredients were prepared and tested for their material properties. The formulations were investigated for potential shrinkage tendency and heat resistance as well as water resistance. Further tests such as resistance to mould infestation and aerobic degradability according to DIN EN ISO 17556 were carried out with the formulation that performed best in the previous tests. Mould resistance was enhanced by adding sodium benzoate. In the laboratory experiment, the CO2 decomposition rate was about 30% after seven weeks. With the favouritised variant, which was found step by step through the experiments, an outdoor field test was carried out to investigate the durability under natural conditions over the vegetation period. In the field, the mulch material maintained its function for six months. In the greenhouse, the effect of the mulch material on weeds was studied. It was found that the mulch material showed a high efficiency in controlling above-ground biomass of Elymus repens, Amaranthus retroflexus, and Setaria viridis. In addition, the biomass of the roots of Amaranthus retroflexus and Elymus repens was reduced. Further studies are on the way to elucidate field suitability and the weed suppressive effect under different environmental conditions.