Dimethyl Sulphoxide Extracts Research Articles

IN VITRO ANTIOBESITY ACTIVITY OF SOME PLANTS THROUGH A MODIFIED LIPASE INHIBITION ASSAY

The objective of the present study was to evaluate the antiobesity potential of various plant (leaves or seed) extracts through a modified in vitro lipase inhibitory activity assay. Dimethyl sulphoxide (DMSO, negative control or solvent) extracts as cold infusion of leaves and seeds of some plants were studied for lipase inhibitory potential using porcine pancreatic lipase enzyme, p- nitro phenyl acetate and orlistat (as positive control or standard inhibitor). Among the leaves, the Urtica dioica showed the best pancreatic lipase inhibition activity (52.0 %). On the other hand, among the seeds, Trachyspermum ammi showed the highest per cent lipase inhibition (91.68 %). Among six leaves’ and seven seeds’ extract, it was evident that the seeds showed better pancreatic lipase inhibition activity over the leaves in the study. The lipase inhibition was found to be in the range of 34.43 to 91.68 % for the plants in study. DMSO extract of the plants under the study showed significant pancreatic lipase inhibitory activity indicating strong antiobesity activity. Therefore, the plants can be further investigated for the identification and isolation of chief bioactive constituents for developing the lead molecules for obesity treatment.

Ehretia laevis leaves: Potential herbal remedy for mouth microflora

The advantages and uses of folklore herbs have been acclaimed and executed from ancient times in India. The use of these Folklore remedial herbs for therapeutic applications is contributing to maintenance of human health. The ancient literature, i.e., Ayurveda and Unani, also describes the global usage of herbal medicine for treatment, and its formulation’s concoction for prevention of various diseases. Ehretia laevis is a conventional therapeutic herb from ancient times, frequently designated as Khandu Chakka by local people in Maharashtra. E. laevis leaves are used in the treatment of skin infections, fungal infections, mouth blisters, eczema, cuts and wounds, diabetes, asthma, fever and joint pain etc. The leaf of this plant contains abundant therapeutically beneficial secondary metabolites besides primary metabolites. This paper describes antimicrobial sensitivity of E. laevis leaf (fresh and dried) acetone and ethyl alcohol (95%) soxhlet extract and dried leaf dimethyl sulphoxide (DMSO) extract (prepared after from evaporation of acetone in dried leaf acetone extract) against isolated oral microbial flora i.e., Streptococcus spp, Staphylococcus aureus and Candida spp. Amoxicillin in dimethyl sulphoxide was also tested for antimicrobial sensitivity. The result revealed that E. laevis fresh leaf ethyl alcohol and acetone extract and dried leaf acetone and DMSO extract was efficacious against isolated oral microbial flora. The extracts showed positive results for flavonoids and tannins. The results showed the antibacterial and antifungal potential of this folklore plant, particularly against S. aureus and Candida spp., which are microorganisms that are becoming resistant against most therapeutic drugs. This use of this folklore herb requires further study on pharmacological drug formulations. And it can also be used in herbal products i.e., toothpastes, mouthwash etc.

Dye yielding potential and antibacterial properties of lichens from Kerala, India

Lichens are a group of lower plants that adapt to extreme environmental conditions. The utility of lichens is due to the wide range of secondary metabolites produced by them. The present study is to analyse the dye yielding potential and anti-bacterial properties of lichen species Heterodermia boryi , Hypotrachyna cirrhata , and Cladonia fruticulosa collected from different parts of Kerala. Dyes were extracted using methods such as Ammonia fermentation method (AFM), Di-methyl sulphoxide extraction method (DEM) and the boiling water method (BWM). The dye extracts were experimented on mulberry silk and banana fibre. The fibre stability was tested by exposure to sunlight and action with detergent and shampoo. Most of the dyed colours appeared to be stable. The colours were detected using COSMIN software. The colours that appeared were mainly shades of brown, khaki, tan and gold. AFM and DEM are better extraction methods than BWM. To investigate the antibacterial activity, methanol, ethanol, acetone, chloroform and distilled water extracts of lichens were tested against Gram-positive and negative bacteria along with positive control. Chloroform and acetone extracts showed exceptional inhibition activity followed by methanol and ethanol and the least activity by distilled water extracts. Extracts of H. cirrhata and C. fruticulosa showed better antibacterial activity than H. boryi . These results indicate the presence of different secondary metabolites.

Pharmacognostical and Antimalarial Studies of Tamarindus indica Leaves

The present study was performing to know the Antimalarial activity of Tamarind (Tamarindus indica L.) The plant T. indices were collected further by using methanol the extract residue diluted 10% Dimethyl sulphoxide extract from which were produced. The plate diffusion method was used as an antimicrobial test for Plasmodium falciparum and Plasmodium vivax by a zone of inhibition tested.TLC standardization ensures the presence of tartaric acid in the extracted sample against the standard. leaves possess near to standard Antimalarial activity which was confirmed by its effect on experimental living organisms.

Wet milling of buckwheat cultivars and some quality properties of the fractions.

The hulled buckwheat cultivars (Aktaş cv. and Güneş cv.) were wet-milled, and then some chemical, yields, colour, functional properties, phenolic compound, antioxidant activity, and pasting, thermal and retrogradation properties of starches were investigated and compared with the wholegrain buckwheat flour (with hull) and buckwheat groat flour (without hull) of the same cultivars. Sodium dodecyl sulphate polyacrylamide gel electrophoresis patterns of flours and protein fractions were examined under reducing and non-reducing conditions. The hull, germ+dietary fibre, protein and starch fractions were collected. The total recovery for Aktaş cv. and Güneş cv. cultivars were 98.1% and 96.1%; total starch yields were 51.6% and 49.7%; pasting temperatures of the starches were found as 83.7 and 85.7°C; and final viscosities of starches were determined as 3.5 and 3.4 Pa·s, respectively. The resistant starch contents of starch fractions of Aktaş cv. ve Güneş cv. were found as 3.28% and 3.62%, respectively. The highest total phenolic compound contents were detected with dimethyl sulphoxide extraction in the germ+dietary fibre fractions. The highest 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity and trolox equivalent antioxidant capacity were found in the hull fraction (as 81.7%) and germ+dietary fibre fraction (as 11.8 mmol/kg) of Aktaş cv. cultivar.

Ecotoxicity testing of airborne particulate matter\u2014comparison of sample preparation techniques for the Vibrio fischeri assay

The bioassay based on the bioluminescence inhibition of the marine bacterium Vibrio fischeri has been the most widely used test for the assessment of airborne particulate matter ecotoxicity. Most studies available use an extract of the solid sample, either made with water or organic solvents. As an alternative, a whole-aerosol test is also available where test bacteria are in actual contact with contaminated particles. In our study, different extraction procedures were compared to this direct contact test based on the V. fischeri assay and analytical measurements. The lowest PAH content and the highest EC50 were determined in water extract, while the highest PAH amount and lowest EC50 were measured in dichloromethane, hexane, and dimethyl-sulphoxide extracts. EC50 of the direct contact test was comparable to that of the methanol extract. Our results suggest that the sensitivity of the direct contact test equals to that of extraction procedures using organic solvents, moreover, it is mimicking an environmentally realistic exposure route.

FORMULATION AND EVALUATION OF MULTIPURPOSE HERBAL CREAM

Objective: To formulate and evaluate herbal cream using Aloe Vera gel, dimethyl sulphoxide extracts of Neem (Azadirachta indica) and Tulsi (Ocimumtenuiflorum) to give multipurpose effect.
 Methods: The cream was prepared by using the cream base that is bee’s wax, liquid paraffin, borax, methylparaben, distilled water, rose oil, Aloe Vera gel, dimethyl sulphoxide extracts of Neem and Tulsi. The cream was prepared by using the slab technique/extemporaneous method for geometric and homogenous mixing of all the excipients and the herbal extracts. By using slab technique, we have developed three batches of our herbal cream, namely F1H, F2H, and F3H. All three batches were evaluated for different parameters like appearance, PH, viscosity, phase separation.
 Results: All the three formulations F1H, F2H, F3H showed good appearance, PH, adequate viscosity and no phase separation was observed. Also, the formulations F1H, F2H, F3H showed no redness, erythema and irritation during irritancy study and they were easily washable. All the three formulations F1H, F2H, F3H were stable at room temperature.
 Conclusion: All three herbal ingredients showed significant different activities. Based on the results, we can suggest that all the three formulations F1H, F2H, F3H were stable and can be safely used on the skin.

An Optimised Method for Routine Separation and Quantification of Major Alkaloids in Cortex Cinchona by HPLC Coupled with UV and Fluorescence Detection.

Authentication of herbal products to ensure efficacy and safety require efficient separation and quantification of constituents. Standard assays for Cinchona bark used for the treatment of malaria and production of quinine, either use only spectrophotometry to detect two pairs of diastereoisomers of quinine and cinchonine type alkaloids (European Pharmacopoeia, Ph.Eur.) or liquid chromatography primarily optimised for detection of the four major alkaloids. However, numerous minor alkaloids occur in Cinchona and related species and efficient separation including gradient elution is necessary in order to obtain the full pattern of constituents in bark samples. To develop an optimised HPLC method for separation and quantitative analysis of the four major alkaloids in Cinchona bark using UV detection. Dimethyl sulphoxide (DMSO) extracts of 50mg of pulverised barks were prepared using ultrasonication. The chromatographic separation was performed on an XB-C18 column packed with 2.6μm particles. Gradient elution using an ammonium formate buffer and methanol as organic modifier over 26min was based on non-chiral separation of the diastereoisomers and the high solvent selectivity of methanol. Post column UV detection was performed at 250nm and 330nm. Fluorescence detection was performed using 330nm for excitation and 420nm for emission. The optimised HPLC method facilitates efficient separation and quantification of the four major alkaloids in 26min with a limit of quantification of 5μg/g from 50mg bark sample. The optimised HPLC method offers a simple and efficient quantification of the four major alkaloids. Copyright © 2017 John Wiley & Sons, Ltd.

Mutagenic activities of biochars from pyrolysis

Biochar production, from pyrolysis of lignocellulosic feedstocks, agricultural residues, and animal and poultry manures are emerging globally as novel industrial and commercial products. It is important to develop and to validate a series of suitable protocols for the ecological monitoring of the qualities and properties of biochars. The highly sensitive Salmonella mutagenicity assays (the Ames test) are used widely by the toxicology community and, via the rat liver extract (S9), can reflect the potential for mammalian metabolic activation. We examined the Ames test for analyses of the mutagenic activities of dimethylsulphoxide (DMSO) extracts of biochars using two bacterial models (S. typhimurium strains TA98 and TA100) in the presence and in the absence of the metabolic activation with the S9-mix. Tester strain TA98 was most sensitive in detecting mutagenic biochar products, and the contribution of S9 was established. Temperature and times of pyrolysis are important. Biochar pyrolysed at 400°C for 10min, from a lignocellulose precursor was mutagenic, but not when formed at 800°C for 60min, or at 600°C for 30min. Biochars from poultry litter, and manures of calves fed on grass had low mutagenicities. Biochar from pig manure had high mutagenicity; biochars from manures of cows fed on a grass plus cereals, those of calves fed on mother's milk, and biochars from solid industrial waste had intermediate mutagenicities. The methods outlined can indicate the need for further studies for screening and detection of the mutagenic residuals in a variety of biochar products.

Assessment of Bioautography and Spot Screening of TLC of Green Tea (Camellia) Plant Extracts as Antibacterial and Antioxidant Agents.

This study was carried out as a prerequisite to evaluate the therapeutic potential of Camellia varieties. The crude extracts of six different plants of green tea Camellia assamica and Camellia sinensis were tested against three Gram-positive and four Gram-negative bacteria using agar disk diffusion method at 50 mg/ml concentration. 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) and diphenyl-(2,4,6-trinitrophenyl)iminoazanium free radical scavenging methods were performed to evaluate the antioxidant potential. Phytochemical constituents and trace metals were detected through thin layer chromatography and Inductively Coupled Plasma Atomic Emission Spectrophotometer, respectively. The maximum inhibition of Staphylococcus aureus was recorded by dimethyl sulphoxide extracts of green tea varieties. The measured zone of inhibition of dimethyl sulphoxide extracts by Qimen was (10.00±0.0 mm), Japanese (10.00±0.0 mm), Turkish (10.00±0.0 mm), Indonesian (8.33±1.0 mm), P3 clone (10.00±0.0 mm) and Sri Lankan (10.00±0.0 mm). Maximum scavenging potential activity was found with ethanol, methanol and dimethyl sulphoxide extracts. Spot screening of TLC-developed plates indicated that the presence of active biological compounds such as flavonoids, proteins, phenols, alkaloids and glycosides also exhibited strong activity against tested bacterial strains. This study reveals the potential biological activities of Camellia assamica and Camellia sinensis having massive phytochemical constituents and trace elements.

Evaluating antioxidant property of brown ALGA Colpomenia sinuosa (DERB. ET SOL)

The brown alga, Colpomenia sinuosa was subjected to DPPH (1,1-diphenyl-2-picryl hydrazyl), nitric oxide radical, hydrogen peroxide, scavenging of ABTS radical and Total antioxidant capacity (TAC) inhibitory assays to assess its antioxidant property. The dimethyl sulphoxide (DMSO) and methanol extracts showed highest antioxidant activity in DPPH (96.56 and 88.57%), nitric oxide radical (81.2 ± 4.1% and 76.69 ± 3.1 %), hydrogen peroxide (70.7 ± 3.5 and 56.6 ± 2.0%) and ABTS (76.8 ± 3.8% and 74.0 ± 2.8%). The moderate activity was observed in total antioxidant capacity (60.3 ± 4.3 and 57.0 ± 2.0) assay. The activity was higher and comparable to that of commercial antioxidants butylated hydroxy anisole (BHA) (87.38 ± 1.32%) and butylated hydroxy tolune (BHT) (56.05 ± 0.19%) at 2 mg/ml concentration. The significant free radical scavenging activity of C. sinuosaindicated that it could be a potential source for natural antioxidant lead molecules. Key words: ABTS, Colpomenia sinuosa, DPPH, hydrogen peroxide, nitric oxide radical.

Bacterial growth inhibition potential of annatto plant parts

ObjectiveTo study the antibacterial efficacy of Bixa orellana leaves and deseeded fruit capsule extracts against both Gram positive and Gram negative bacteria. MethodsThe antibacterial activity of the ethanolic, methanolic, acetone and dimethyl sulphoxide extracts of B. orellana were tested against Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Bacillus subtilis, Bacillus cereus and Staphylococus aureus by disc diffusion method. ResultsThe antibacterial activity of leaf was more pronounced even at low concentrations and fruit extracts exhibited the same at relatively higher concentrations. Only DMSO extract of seeds showed growth inhibition of S. aureus, B. subtilis, B. cereus, and P. aeruginosa. ConclusionsThe present study suggested that the leaves and deseeded capsule extracts of B. orellana possess significant antibacterial activity thereby providing substantial support for the ethanobotanical applications of this plant.

Identification of a Candida parapsilosis Strain Producing Extracellular Serine Peptidase with Keratinolytic Activity

A yeast strain isolated from feather waste from a chicken processing plant was identified as Candida parapsilosis by biochemical tests and morphological studies. The yeast was able to grow in phosphate-buffered saline supplemented with 1% native feather as the sole carbon and nitrogen source. A keratin substrate was obtained from the feathers by dimethylsulphoxide extraction. A 20-fold concentrated culture supernatant from Candida parapsilosis grown on feathers was analysed by SDS-PAGE electrophoresis containing either 1% gelatin or 1% keratin as copolymerised substrates. The presence of a single band with an approximate molecular mass of 60 kDa with gelatinolytic and keratinolytic activities was observed. This proteolytic activity was fully inhibited by phenylmethylsulphonyl fluoride. These results suggest that the extracellular enzyme belongs to the serine peptidase class. This is the first report of an extracellular serine peptidase produced by C. parapsilosis with keratinolytic activity. The role of this enzyme in yeast-host interactions is discussed.

Alkali insoluble glucan extracted fromAcremonium diospyriis a more potent immunostimulant in the Indian White Shrimp,Fenneropenaeus indicusthan alkali soluble glucan

Effect of an extraction method on the structure of glucan and its immunostimulatory response in Fenneropenaeus indicus was investigated. Here we extracted alkali insoluble glucan (AIG) and alkali soluble glucan (ASG) from a filamentous fungi Acremonium diospyri following alkali–acid hydrolysis and the sodium hypochlorite oxidation and dimethyl sulphoxide extraction method respectively. Structural analysis showed that 85% of glucan in AIG was a (1→3)-β-d-glucan and it increased the prophenoloxidase and reactive oxygen intermediate activity when administered to F. indicus. On the other hand, ASG, which contained 93% (1→3)-α-glucan, did not induce significant immune response in shrimp. Here we report that the difference in immunostimulatory potential between AIG and ASG is due to the difference in the percentage of (1→3)-β-d-glucans present in each preparation, which varies with the method of extraction employed. Also our observations suggest that glucan can be used as a potential immunostimulant to shrimp, provided it contains (1→3)-β-d-glucan as the major fraction.

Nondestructive and Rapid Estimation of Leaf Chlorophyll and Nitrogen Status of Peace Lily Using a Chlorophyll Meter#

Timely and nondestructive detection of leaf nitrogen (N) status could greatly improve nutrient management practices for greenhouse ornamental production. This study evaluated the feasibility of using a SPAD-502 chlorophyll meter for estimating leaf chlorophyll and nitrogen contents of the peace lily (Spathiphyllum Schott). Three cultivars ‘Claudia’, ‘Double Take’, and ‘Petite’ were grown in a soilless substrate and sub-irrigated with solutions containing N at 50, 100, 200, or 400 mg L−1 through an ebb-and-flow system. The SPAD-502 chlorophyll meter was used to measure leaf greenness. Chlorophyll contents of leaves were also analyzed using the dimethyl sulphoxide extraction method. Total N was determined by a CNS Auto-Analyzer. Correlation analyses showed that coefficients (r 2) between SPAD values and leaf chlorophyll contents were 0.83, 0.77, and 0.73 for ‘Claudia’, ‘Double Take’, and ‘Petite’, respectively, and coefficients (r 2) between SPAD values and leaf N contents of ‘Claudia’, ‘Double Take’, and ‘Petite’ were 0.84, 0.82, and 0.91, respectively. These results suggest that the readings from a SPAD meter can be used for rapidly and nondestructively estimating leaf chlorophyll and N status of the peace lily. As high quality Spathiphyllum were produced at the N concentration of 200 mg L−1, the corresponding SPAD values established in this study could facilitate in situ decision-making on N application for the production of the peace lily. #This research was supported by the Florida Agricultural Experiment Station and approved for publication as Journal Series No. R-09531.

Correlation of 32P-postlabelling-detection of DNA adducts in mouse skin in vivo with the polycyclic aromatic compound content and mutagenicity in Salmonella typhimurium of a range of oil products.

The in vivo genotoxic activities in mouse skin of the dimethyl sulphoxide (DMSO) extracts of a range of oil products [residual aromatic extract; untreated heavy paraffinic distillate aromatic extract; mildly refined light naphthenic base oil; bitumen (vacuum residue); high viscosity index base oil obtained by catalytic hydrogenation] were evaluated by 32P-postlabelling DNA analysis. The results of quantitative 32P-postlabelling analyses of epidermal DNA from mice treated with the DMSO extracts showed linear relationships with the total polycyclic aromatic compound (PAC) contents, determined by the Institute of Petroleum method IP 346 and also the 3-6 ring PAC contents, measured by on-line liquid-liquid extraction using flow injection analysis. The 32P-postlabelling data also showed a linear relationship with the mutagenicity indices of these oil products determined in S. typhimurium TA98 using the modified Ames Salmonella microsome test. The in vivo genotoxicity of the DMSO extracts from the oil products was low, judged by 32P-postlabelling analysis of DNA adducts measured in epidermal DNA of treated mouse skin, and ranging from 2 to 723 attomole/microg DNA per mg oil product. The in vivo 32P-postlabelling data from this study are consistent with these materials expressing low genotoxicity in mouse skin in vivo. The DMSO extraction procedure coupled with 32P-postlabelling DNA analysis is useful for ranking the relative genotoxic potency in vivo of a wide range of oil products. In general the trend observed is similar to rankings based on physicochemical measurements of total PAC contents or 3 6 ring PAC contents of the oil products.

Detection of mutagenic potential of some glass-ionomer cements through Ames testing.

The mutagenic potential of three commercially available glass-ionomer cements used in dentistry was examined. The cement components were mixed according to the manufacturers indications and set for two defined times: 1 h or, alternatively, 1 wk. Cements B and C set spontaneously; in the case of cement A, the manufacturer suggests the use of a lamp to trigger also a photopolymerization. Photopolymerization, however, was not used. Ames tests were performed on the dimethyl sulphoxide extracts of cements by using Salmonella typhimurium strains TA 98, TA 100, TA 1535, TA 1537, TA 1538 and TA 102. Cement A showed mutagenicity only against TA 1537 strain, either in the presence or absence of metabolic activation with microsomial fraction S9. The other two cements showed no mutagenic potential. We conclude that glass-ionomer cements are, on the whole, safe materials from the viewpoint of genotoxicity, and hypothesize that the mutagenicity observed in cement A could depend on its polymerization performed without light activation.

A fourier‐transform infrared spectroscopic and microscopic study of the infection of potato tubers by Erwinia carotovora ssp carotovora in aerobic and anaerobic conditions

AbstractThe infection or potato tubers (Solanum tuberosum) by Erwinia carotovora ssp carotovora in aerobic and anaerobic conditions was studied using chemical analysis and Fourier‐transform infrared (FT‐IR) microscopy and diffuse reflectance spectroscopy. Analysis or the uninfected and infected residues following sequential extraction with diethyl ether, water (40°C), 90% dimethyl sulphoxide (DMSO) and digestion with amyloglucosidase showed that the principal effect of infection, particularly in anaerobic conditions, is starch degradation. This was substantiated by gel filtration chromatography of the 90% DMSO extracts. Extensive lignification was not found to be intimately associated with either infective condition. FT‐IR microscopical analysis of tissue sections showed that infection in both aerobic and anaerobic conditions caused starch and cell wall degradation, whilst extensive membrane degradation was only evident with anaerobic infection.

Propolis extracts are effective against Trypanosoma cruzi and have an impact on its interaction with host cells

Propolis, a natural resin produced by honey bees, that displays strong antimicrobial activity, has been used as a chemotherapeutic agent since ancient times. The anti-protozoan properties of different propolis extracts were studied regarding T. cruzi and its interaction with host cells. Ethanolic (EEP) and dimethylsulphoxide extracts (DEP) were both active against the three forms of the parasite, with the former being more active than the latter against the vertebrate forms, amastigotes and trypomastigotes. Total lysis of bloodstream trypomastigotes was observed after 24 h in the presence of EEP at a concentration of 100 μg/ml. The effect was found to be temperature dependent. Treatment of infected peritoneal macrophages and heart muscle cells with EEP strongly inhibited infection levels. The utilization of propolis as a possible antitrypanosomal agent is discussed.

An investigation of the main mutagenic components of a carcinogenic oil by fractionation and testing in the modified Ames assay.

A low-viscosity carcinogenic oil was extracted with dimethylsulphoxide (DMSO) and the extract fractionated into 1-3 ring aromatic components, 4-6 ring polycyclic aromatic compounds (PCAs) and polar materials. Each of these fractions was tested in the Mobil modified Ames assay at the same concentration as in the DMSO extract, alongside unfractionated DMSO extracts from the same oil and two reference oils. In addition the fractions were combined in various ways to examine inhibitory or additive effects. Clear positive findings were obtained with the DMSO extract of the main test oil, with some reduction in activity in a sample (the starting material for fractionation) that was back-extracted to remove DMSO. Of the three fractions tested on their own and assessed in terms of mutagenicity index, the 1-3 ring aromatic fraction had the greatest mutagenic activity, showing similar activity to the starting material, whereas the 4-6 ring PCA fraction had much lower activity and the polar fraction showed the lowest activity with an absence of doubling in one of the tests. The polar fraction showed signs of an inhibitory effect on the response to the 1-3 ring aromatic fraction and to a questionable extent on that of the 4-6 ring PCA fraction. Although a low level of the 4-6 ring PCA fraction showed some inhibitory effect on the response to the 1-3 ring aromatic fraction, a low level of the 1-3 ring aromatic fraction added to the 4-6 ring PCA fraction enhanced the response.(ABSTRACT TRUNCATED AT 250 WORDS)