Introduction: The organizational citizenship behavior in hospitals is of great importance since it enhances positive relationships between employees and their involvement in organizational activities. On the other hand, culture is one of the main and major components of the organization, which has a direct effect on promoting the organization's activities. In this regard, rich Islamic culture with its pure concepts has a great role in shaping and refining people's behaviors. The purpose of this study was to investigate the effect of Islamic culture on organizational citizenship behavior among nurses in hospitals of Yazd University of Medical Sciences. Methods: In this cross-sectional study, 264 staff of the selected hospitals affiliated with Yazd University of Medical Sciences was selected using two-stage sampling method. The data collection tool was a valid and reliable questionnaire consisting of three sections: demographic information, a 22-item organizational citizenship behavior questionnaire with 5 dimensions, and the Islamic culture questionnaire, which should be answered using a 5-point Likert scale. Data were analyzed using SPSS 21 and SmartPLS software. Results: Among the dimensions of organizational citizenship behavior, the highest mean and standard deviation were related to conscientiousness dimension (3.10±0.69). The total mean and standard deviation was calculated as 2.99±0.57 for the component of organizational citizenship behavior. The mean and standard deviation of Islamic culture was 2.89±0.53. According to the data analysis algorithm in PLS method, by examining the significant coefficients of t for each path, Islamic culture had significant effect on all components of the organizational citizenship behavior. Conclusion: Considering the impact of Islamic culture on organizational citizenship behavior, various cultural programs such as cultural training courses, cultural seminars, and cultural events are suggested to promote Islamic culture in hospitals
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