The purpose of this paper is to identify the determinants of organizational culture that stimulate innovation and the strength of their effect and to provide more methodological evidences that are addressing organizational cultural determinants, which stimulate or imped innovation in Commercial Companies. Based on the responses of the study sample that included 397 employees in (11) Palestinian companies, their age varied from 25 to more than 50 years old with administrative and executive positions, the results highlighted the role of organizational culture in stimulating innovation, thus helping the organization to achieve innovation. The study showed that there was a significant effect of organizational culture dimensions on innovation. Therefore, large- and medium commercial companies must take general, clear, and time-scheduled strategic and structural actions to promote and address the shortcoming in their customer focus levels, continued organizational learning, empowerment and clarity of changing work environment to enhance sharing knowledge to ensure an effective balance. In addition, it is necessary to emphasize interest in the development programs to gather and attract innovative workers.