Dim Light Research Articles

42‐2: Self‐Scalable UV Blocking Artificial Iris Operated by Radially Controlled Crosslinking Density with Fast Switching Dynamics

In the anatomy of human eye, the role of iris is crucial since it controls the light level entering the eye and enhances the visibility. Here, we propose a soft contact lens type artificial iris actuated by eternal UV (ultra‐violet) light source, self‐regulating its effective aperture size by varying the crosslinking density of bio‐compatible polymer matrix doped with photochromic dye. For its self‐regulating characteristic, we cured HEMA‐based polymer matrix gradually from center to edge. Since the polymer matrix is polymerized by heat, we utilized NIR (near infra‐red) lamp and gradient grayscale patterned photomask for its gradual characteristic. Thereby, the crosslinking density of central position is higher compared to that of peripheral, causing slow switching dynamics. The gradient difference in switching dynamics leads to artificial iris gaining self‐regulation dynamics of effective aperture. The manufactured artificial iris shows advantage in blocking UV light, self‐regulating, and fast switching dynamics. The switching dynamics of artificial iris under bright and dim light adaptation are less than 50 seconds and 120 seconds, showing similar property with human iris.

Feasibility and acceptability for LION, a fully remote, randomized clinical trial within the VA for light therapy to improve sleep in Veterans with and without TBI: An MTBI2 sponsored protocol.

Sleep-wake disturbances frequently present in Veterans with mild traumatic brain injury (mTBI). These TBI-related sleep impairments confer significant burden and commonly exacerbate other functional impairments. Therapies to improve sleep following mTBI are limited and studies in Veterans are even more scarce. In our previous pilot work, morning bright light therapy (MBLT) was found to be a feasible behavioral sleep intervention in Veterans with a history of mTBI; however, this was single-arm, open-label, and non-randomized, and therefore was not intended to establish efficacy. The present study, LION (light vs ion therapy) extends this preliminary work as a fully powered, sham-controlled, participant-masked randomized controlled trial (NCT03968874), implemented as fully remote within the VA (target n=120 complete). Randomization at 2:1 allocation ratio to: 1) active: MBLT (n=80), and 2) sham: deactivated negative ion generator (n=40); each with identical engagement parameters (60-min duration; within 2-hrs of waking; daily over 28-day duration). Participant masking via deception balanced expectancy assumptions across arms. Outcome measures were assessed following a 14-day baseline (pre-intervention), following 28-days of device engagement (post-intervention), and 28-days after the post-intervention assessment (follow-up). Primary outcomes were sleep measures, including continuous wrist-based actigraphy, self-report, and daily sleep dairy entries. Secondary/exploratory outcomes included cognition, mood, quality of life, circadian rhythm via dim light melatonin onset, and biofluid-based biomarkers. Participant drop out occurred in <10% of those enrolled, incomplete/missing data was present in <15% of key outcome variables, and overall fidelity adherence to the intervention was >85%, collectively establishing feasibility and acceptability for MBLT in Veterans with mTBI.

The dark side of apnea: altered 24-hour melatonin secretion in obstructive sleep apnea (OSAS) is disease severity dependent

PurposeMelatonin aids in the synchronization of the circadian rhythm to the external environment. Few studies have tried to elucidate the relationship between melatonin and obstructive sleep apnea syndrome (OSAS). These often include few patients, do not differentiate between OSAS severity and/or do not analyse a 24-h melatonin profile. This study set out to investigate disease severity dependent differences in 24-h salivary melatonin secretion of OSAS patients compared to a reference population in a retrospective design.Methods24-h salivary melatonin profiles of 169 OSAS patients were analysed (55 light, 66 moderate, 48 severe) as well as 91 reference patients. Several aspects of the melatonin curve were analysed and stratified according to OSAS severity. Parameters included: dim light melatonin onset (DLMO), time of returning below DLMO (DLMOoff), peak melatonin concentration and time, and total melatonin exposure.ResultsSignificant effects were corrected for confounding by age and sex using linear regression. Our analysis shows that, compared to reference and in a disease dependent manner, OSAS patients have a significantly lower 24-h melatonin curve, lower melatonin peak concentration, lower total melatonin exposure and a smaller proportion of patients reach DLMO. The differences in peak melatonin production and total melatonin exposure were resistant to confounding by age and/or sex.ConclusionThis study describes clear OSAS severity dependent abnormalities in melatonin production in OSAS patients, independent of sex and/or age. Future research should indicate whether oral melatonin supplementation has beneficial effects in OSAS patients with attenuated endogenous melatonin production.

Enhancement of Mine Images through Reflectance Estimation of V Channel Using Retinex Theory

The dim lighting and excessive dust in underground mines often result in uneven illumination, blurriness, and loss of detail in surveillance images, which hinders subsequent intelligent image recognition. To address the limitations of the existing image enhancement algorithms in terms of generalization and accuracy, this paper proposes an unsupervised method for enhancing mine images in the hue–saturation–value (HSV) color space. Inspired by the HSV color space, the method first converts RGB images to the HSV space and integrates Retinex theory into the brightness (V channel). Additionally, a random perturbation technique is designed for the brightness. Within the same scene, a U-Net-based reflectance estimation network is constructed by enforcing consistency between the original reflectance and the perturbed reflectance, incorporating ResNeSt blocks and a multi-scale channel pixel attention module to improve accuracy. Finally, an enhanced image is obtained by recombining the original hue (H channel), brightness, and saturation (S channel), and converting back to the RGB space. Importantly, this image enhancement algorithm does not require any normally illuminated images during training. Extensive experiments demonstrated that the proposed method outperformed most existing unsupervised low-light image enhancement methods, qualitatively and quantitatively, achieving a competitive performance comparable to many supervised methods. Specifically, our method achieved the highest PSNR value of 22.18, indicating significant improvements compared to the other methods, and surpassing the second-best WCDM method by 10.3%. In terms of SSIM, our method also performed exceptionally well, achieving a value of 0.807, surpassing all other methods, and improving upon the second-place WCDM method by 19.5%. These results demonstrate that our proposed method significantly enhanced image quality and similarity, far exceeding the performance of the other algorithms.

Genotype by environment interactions for growth and inner shell color disparity in Hyriopsis cumingii under different light conditions

The light plays a pivotal role in influencing the growth and body color for aquaculture animals. The freshwater pearl mussel Hyriopsis cumingii, inhabiting benthic environments naturally, is predominantly cultivated by hanging methods. This aquaculture method altersmutiple environmental factors, with light being one of the maximum variationfactor. In order to comprehend the impact of light change on the growth and inner shell color of H. cumingii, a total of 14 full-sibling families were established and cultivated separately under natural light and dim light conditions for a period of 13 months in this study. The results revealed that under natural light conditions, juvenile mussel exhibited accelerated growth rates, while dim light conditions expedited purple pigmentation in the inner shell. Genetic analysis indicated general heritability range for the entire experimental population falls between 0.07 and 0.28. heritability estimates for all traits under natural light conditions (NL) ranged from 0.33 to 0.72, indicating consistently high heritability. However, heritability estimates for all traits under dim light conditions (DL) ranged from 0.05 to 0.16. The growth and inner shell color traits showed significant G × E interactions under different light conditions. The anal angle radial rib length (AARRL) trait exhibited a significantly higher correlation with body weight (BW) compared to shell height (SH) and total shell height (TSH). Furthermore, the growth and inner shell color traits of different families were compared and evaluated under the two environmental conditions in this study. Families 1 and 6 excelled in various traits under both conditions, making them promising candidates for genetic improvement breeding. In this study, pronounced influences of light exposure on the growth of pearl mussels were identified, emphasizing the need for tailored breeding strategies for both bottom seeding and suspended cultivation methods.

Fine-tuning vision foundation model for crack segmentation in civil infrastructures

Large-scale foundation models have become the mainstream deep learning method, while in civil engineering, the scale of AI models is strictly limited. In this work, a vision foundation model is introduced for crack segmentation. Two parameter-efficient fine-tuning methods, adapter and low-rank adaptation, are adopted to fine-tune the foundation model in semantic segmentation: the Segment Anything Model (SAM). The fine-tuned CrackSAM shows excellent performance on different scenes and materials. To test the zero-shot performance of the proposed method, two unique datasets related to road and exterior wall cracks are collected, annotated and open-sourced, for a total of 810 images. Comparative experiments are conducted with twelve mature semantic segmentation models. On datasets with artificial noise and previously unseen datasets, the performance of CrackSAM far exceeds that of all state-of-the-art models. CrackSAM exhibits remarkable superiority, particularly under challenging conditions such as dim lighting, shadows, road markings, construction joints, and other interference factors. These cross-scenario results demonstrate the outstanding zero-shot capability of foundation models and provide new ideas for developing vision models in civil engineering.

AI-Driven Dim-Light Adaptive Camera (DimCam) for Lunar Robots

Abstract. The past decade has been a boom in lunar exploration. China, India, Japan and other countries have successfully landed landers or rovers on the lunar surface (Wu et al., 2014, 2018, 2020; Prasad et al., 2023). Future missions to explore the Moon are focusing on the lunar south pole (Peña-Asensio et al., 2024). The solar altitude angle at the lunar south pole is extremely low, resulting in low solar irradiance and large areas often in dim light or shadows. The permanently shadowed regions (PSRs) at the lunar south pole are also likely containing substantial amounts of water ice (Li et al., 2018). Future lunar robots exploring the lunar south pole will need to operate in low light or shadowed regions, making camera sensors sensitive to the dim-light environments necessary for these robots. Common night vision sensors usually use near-infrared cameras. However, sensors based on passive infrared technology have image resolution limited by several factors, including the intensity of infrared radiation emitted by the object, the sensitivity of the camera, and the performance of the optical system. For instance, thermal imagers typically have a resolution of 388*284 pixels only. We have developed a dim-light adaptive camera (DimCam) that is ultra-sensitive to the varying illumination conditions driven by AI to achieve high-definition imaging of 1080P or above, for future lunar robots operating in shadows or dim-light regions. The DimCam integrates two starlight-level ultra-sensitive imaging sensors connected by a rigid base to provide stereo vision in low illumination environment. An AI edge computing unit is embedded inside the DimCam to adaptively denoise and enhance image quality. The AI module uses an end-to-end image denoising network to identify and remove noises in the images more accurately by utilizing depth information from the stereo sensors. Compared with traditional monocular denoising algorithms, the denoising network based on stereo vision can significantly improve denoising effects and efficiency by enhancing the signal-to-noise ratio of the data input in the front end. The superposition of overlapping scenes can be regarded as a delayed exposure. Concurrently, the residual analysis of the aligned images aids in noise identification. In addition, for pixels obscured by noise, more accurate pixel values can be restored through interpolation or replacement using depth information obtained from the stereo sensors. Subsequently, a pre-trained lightweight deep network modified from Zero-DCE (Guo et al., 2020) is used for image quality enhancement in terms of brightness and contrast, providing high-quality images even in low-light environments for subsequent applications, such as positioning and navigation of robots, 3D mapping of the surrounding environment, and autonomous driven. We have tested the DimCam in a simulated environment in the laboratory, and the results show that the DimCam has promising performances and great potential for various applications.

Attention and sensory integration for gait in young adults with autism spectrum disorder

IntroductionAltered sensorimotor function is a common feature of autism spectrum disorder (ASD). As a result, spatiotemporal walking patterns are typically affected. Attentional processes relevant for locomotion may be altered in people with ASD. This study assessed the extent to which gait alterations observed under sensory challenging conditions are due to reduced attention-related processes in young adults with ASD. MethodsTwenty-one adults with ASD and 21 age- and sex-matched neurotypical participants walked at a self-selected pace on a 10-m walkway under 12 sensory/attention conditions: hard or carpet flooring; well-lit or dim lighting; no attention task, an auditory choice-reaction time information-processing task, or a simple reaction time information-processing task. Gait data were collected with a 12-marker motion capture set and a trunk accelerometer. Spatiotemporal characteristics of gait were derived and compared between the two groups across gait conditions. ResultsFloor/light conditions impacted gait speed, average step length, average stance time, average step width, and step width variability similarly in both groups (p<0.05). The information processing tasks impacted average step length, gait speed, and step length variability (p<0.05). Group differences were found in step length metrics: the ASD group had decreased average step length during the simple reaction time information-processing task and neurotypical participants did not (p=0.039); the ASD group had increased variability on carpet compared to hard floor and the neurotypical group had no change in variability due to floor (p=0.015). SignificanceThese results suggest that attentional set-shifting and somatosensory inputs may play an important role in ASD-related gait alterations. Step length metrics appear to be sensitive to group differences between ASD and neurotypical adults during sensory challenging conditions.

Regulation of Green Fluorescent Proteins by Sea Anemones (Anthopleura spp.) in Response to Light.

Green Fluorescent Proteins (GFPs) are a family of proteins with a disjunct systematic distribution; their biological functions remain speculative for the most part. Here we report studies of 3 closely related species of green sea anemones (Anthopleura) that express GFPs throughout their ectoderm. Individuals of these species maintain facultative symbiosis with zooxanthellae in their endoderm and inhabit the rocky intertidal or shallow subtidal. Thus, they depend on exposure to light to maintain photosynthesis of their symbionts, and simultaneously need to manage stresses associated with this exposure. We present experimental evidence that these sea anemones regulate the amount of GFP in their bodies in response to the surrounding light environment: they increase or reduce GFP when exposed to brighter or dimmer light, respectively, yet they maintain some GFP while in darkness, for surprisingly long periods.

Does cardiovascular autonomic modulation correspond to the increase in dim-light onset of melatonin? A preliminary analysis

Introduction: The endogenous circadian system promotes the rise in melatonin approximately every 24 hours. In the laboratory, dim light melatonin onset (DLMO) is used as the gold standard method to assess circadian phase. Exogenous melatonin affects cardiac sympathovagal balance and vasomotor sympathetic modulation. Whether or not the endogenous increase in melatonin corresponds to changes in cardiovascular autonomic modulation is unclear. Objective: To evaluate if the timing of DLMO corresponds to the timing of changes in cardiovascular autonomic indices. Methods: Five healthy adults (3 females, age 23.8±SD1.8 y) completed a minimum of four nights of sleep regularization by maintaining a self-selected 8h in bed each night before the experiment. The day after regularization, they reported to the laboratory ~8 hours before bedtime. Measurements for melatonin and cardiovascular autonomic modulation started 6 hours before bedtime and continued 1 hour after. Saliva samples were collected every 30 minutes. At the end of every hour, 10 minutes of continuous R-R intervals (R-R) and systolic blood pressure (SBP) were recorded via electrocardiogram and beat-to-beat finger photoplethysmography, respectively. As currently established, DLMO was defined as the interpolated time for melatonin increase over 3pg/ml. Cardiovascular autonomic indexes were run through CardioSeries v.2 to create a temporal series for R-R interval and systolic blood pressure. Cardiac autonomic modulation was assessed by applying the spectral analysis using the fast Fourier transformation method and time domain analysis of heart rate variability (HRV) and spectral analysis of blood pressure variability (BPV). For each autonomic index, data were plotted and fitted with a segmental linear regression to reveal the intercept of the two segments to define the autonomic onset (AO). A paired t-test compared the difference between DLMO and AO times. Bland-Altman plots with a 95% limit of agreement (LOA) identified the distribution of differences between the methods. Additionally, a one-sample t-test compared the differences between methods to a fixed time clock range. Data are shown as mean ± SD and significance was set as p&lt;0.05. Results: AO time calculated for all indexes from HRV and BPV were not statistically different compared to DLMO time (R-R: +16±86 mins, P=0.70; root mean square of successive differences (RMSSD): -20±91 mins, P=0.67; low frequency (LF) nu: +29±52 mins, P=0.74; high frequency (HF) nu: -43±91 mins, P=0.23; LFHF: -26±73 mins, P=0.47; SBP: -14±104 mins, P=0.78; LFSBP: -6±107 mins, P=0.90). The Bland Altman plots did not identify any systematic difference between AO and DLMO times. The smallest difference was LFnu with a LOA 95% from -73 to +131 minutes, while the largest difference was LFSBP with LOA 95% from -217 to +204 minutes. Additionally, the difference between DLMO and AO LFnu times (LFnu: +29±52) was statistically similar from -36 to +95 minutes. Conclusion: In young healthy adults, our preliminary data shows that AO times of cardiovascular autonomic indexes correspond to salivary DLMO. A larger dataset with more frequent simultaneous measurements of melatonin and cardiovascular autonomic modulation are essential to confirm our preliminary findings. Medical Research Foundation of Oregon, OHSU OFDIR, R01HL163232. This is the full abstract presented at the American Physiology Summit 2024 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

Axial length and pharmacologic pupillary dilation in highly myopic patients.

To determine how high myopia impacts pharmacological pupillary dilation, and to evaluate the relationship between the extent of pharmacologic pupillary dilation and axial length. Patients were grouped into high myopes, defined as one or both eyes having a refractive error greater than - 6 diopters, and controls (between - 2 and + 2 diopters). Dilation was achieved with 1 drop each of tropicamide 1% and phenylephrine 2.5%. Pupil size was measured at full and dim light prior to dilation, then 15 and 30min after dilation. Biometry was measured for each patient. Statistical analyses were performed using the Mann-Whitney-Wilcoxon tests, two-sample Welch's t-tests, and linear mixed effect models and generalized estimating equations models accounting for inter-eye correlation. Forty patients (20 high myopes and 20 controls, 80 eyes total) participated in the study. High myopes had larger pupils at baseline and achieved significantly greater pupillary size (7.08mm, 95% CI: 6.97 to 7.19mm) than controls (6.23mm, 95% CI: 5.94 to 6.52mm) after 30min of dilation (P < .0005). Fully dilated pupil size at 30min was significantly correlated with both refractive error (r = - 0.57, P < .0005) and axial length (r = 0.47, P < .0005). Generalized estimating equations and linear mixed effect models identified other predictive variables of pupil size after dilation including age and white-to-white diameter. Highly myopic patients dilate to a larger pupillary size compared to other patients. Predicting dilation based on extent of myopia could facilitate intraocular surgery planning and reduce clinic wait times for myopic patients.

Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization

Photoactivation of the plant photoreceptor and thermosensor phytochrome B (PHYB) triggers its condensation into subnuclear membraneless organelles named photobodies (PBs). However, the function of PBs in PHYB signaling remains frustratingly elusive. Here, we found that PHYB recruits PHYTOCHROME-INTERACTING FACTOR 5 (PIF5) to PBs. Surprisingly, PHYB exerts opposing roles in degrading and stabilizing PIF5. Perturbing PB size by overproducing PHYB provoked a biphasic PIF5 response: while a moderate increase in PHYB enhanced PIF5 degradation, further elevating the PHYB level stabilized PIF5 by retaining more of it in enlarged PBs. Conversely, reducing PB size by dim light, which enhanced PB dynamics and nucleoplasmic PHYB and PIF5, switched the balance towards PIF5 degradation. Together, these results reveal that PB formation spatially segregates two antagonistic PHYB signaling actions – PIF5 stabilization in PBs and PIF5 degradation in the surrounding nucleoplasm – which could enable an environmentally sensitive, counterbalancing mechanism to titrate nucleoplasmic PIF5 and environmental responses.

Dim light at night shifts microglia to a pro-inflammatory state after cerebral ischemia, altering stroke outcome in mice

Circadian rhythms are endogenous biological cycles that regulate physiology and behavior and are set to precisely 24-h by light exposure. Light at night (LAN) dysregulates physiology and function including immune response; a critical component that contributes to stroke pathophysiological progression of neuronal injury and may impair recovery from injury. The goal of this study is to explore the effects of dim LAN (dLAN) in a murine model of ischemic stroke to assess how nighttime lighting from hospital settings can affect stroke outcome. Further, this study sought to identify mechanisms underlying pathophysiological changes to immune response after circadian disruption. Male and female adult Swiss Webster (CFW) mice were subjected to transient or permanent focal cerebral ischemia, then were subsequently placed into either dark night conditions (LD) or one night of dLAN (5 lx). 24 h post-stroke, sensorimotor impairments and infarct sizes were quantified. A single night of dLAN following MCAO increased infarct size and sensorimotor deficits across both sexes and reduced survival in males after 24 h. Flow cytometry was performed to assess microglial phenotypes after MCAO, and revealed that dLAN altered the percentage of microglia that express pro-inflammatory markers (MHC II+ and IL-6) and microglia that express CD206 and IL-10 that likely contributed to poor ischemic outcomes. Following these results, microglia were reduced in the brain using Plexxikon 5622 (PLX 5622) a CSFR1 inhibitor, then the mice received an MCAO and were exposed to LD or dLAN conditions for 24 h. Microglial depletion by PLX5622 resulted in infarct sizes that were comparable between lighting conditions. This study provides supporting evidence that environmental lighting exacerbates ischemic injury and post-stroke mortality by a biological mechanism that exposure to dLAN causes a fundamental shift of activated microglial phenotypes from beneficial to detrimental at an early time point after stroke, resulting in irreversible neuronal death.

Understanding Perspectives on Presbyopia and Use of Pilocarpine HCl 1.25% Twice Daily from Participants of the Phase 3 VIRGO Study.

The phase 3, randomized, vehicle-controlled, 14-day VIRGO study evaluated the efficacy and safety of twice-daily dosing of pilocarpine hydrochloride ophthalmic solution 1.25% (Pilo) in presbyopia. On VIRGO exit, a companion study was conducted to assess the patient experience with presbyopia and satisfaction with Pilo. Recruited individuals completed the Presbyopia Patient Satisfaction Questionnaire (PPSQ) plus a three-part exit survey, or a live interview. The PPSQ evaluated respondents' experience with Pilo. Survey parts 1 and 2 evaluated experience managing presbyopia before and during VIRGO, respectively; part 3 assessed future possibilities of using Pilo in real-world situations. The interview further informed the interviewees' experience with presbyopia and Pilo. The primary endpoint was responders (%) in each rating category of the PPSQ items 1-7; the secondary endpoints were summary of categorical (survey) and qualitative (interviews) responses. The PPSQ and survey included 62 participants who received Pilo (N = 28) or vehicle (N = 34) in VIRGO; the interview included ten participants (Pilo, N = 4; vehicle, N = 6). Per the PPSQ, 64.3% of Pilo users reported vision improvement, including 17.9% with complete improvement; ≥ 46.4% were satisfied/very satisfied with their ability to perform daily activities, see up close unaided, and read in dim light. Among vehicle users, these percentages were 35.3%, 0%, and ≤ 23.5%, respectively. In both subgroups, ≥ 67.9% were interested in using Pilo or Pilo and eyeglasses/contact lenses in the future. Per the interview, vehicle users (n = 6/6) found the eyedrop easy to use but none experienced meaningful near-vision improvements, stopped using other correction method(s) part of the day, were satisfied with the eyedrop, preferred it over their previous correction method(s), or would continue using it if prescribed. Conversely, 75% (n = 3/4) of Pilo users responded positively to each of these six criteria. Findings validate the VIRGO results and improve our understanding of the patient experience, demonstrating improved vision and satisfaction with Pilo (vs. vehicle) when performing daily activities.

0678 Genetic Variants Associated with Dim Light Melatonin Onset in a Delayed Sleep-Wake Phase Disorder Cohort

0678 Genetic Variants Associated with Dim Light Melatonin Onset in a Delayed Sleep-Wake Phase Disorder Cohort

Slender loris optimization algorithm for solving optimization problems

Over the years, numerous optimization problems have been addressed utilizing meta-heuristic algorithms. Continuing initiatives have always been to create and develop new, practical algorithms. This work proposes a novel meta-heuristic approach employing the slender Loris optimization algorithm (SLOA), miming slender Loris behavior. The behavior includes foraging, hunting, migration and communication with each other. The ultimate goal of the devised algorithm is to replicate the food-foraging behaviour of Slender Loris (SL) and the quick movement of SL when threatened (i.e.) their escape from predators and also mathematically modelled the special communication techniques of SL using their urine scent smell. SLOA modelled SL’s slow food foraging behaviour as the exploitation phase, and moving between the tree and escaping from a predator is modelled as the exploration phase. The Eyesight of slender Loris plays a vital role in food foraging during nighttime in dim light. The operator’s Eyesight is modelled based on the angle of inclination of SL. The urine scent intensity is used here to be instrumental in preventing already exploited territory activities, which improves algorithm performance. The suggested algorithm is assessed and tested against nineteen benchmark test operations and evaluated for effectiveness with standard widely recognized meta-heuristics algorithms. The result shows SLOA performing better and achieving near-optimal solutions and dominance in exploration–exploitation balance in most cases than the existing state-of-the-art algorithms.

Alteration of circadian sleep-wake rhythm and salivary melatonin secretion in idiopathic/isolated REM sleep behavior disorder: Preliminary evidence

Objective/BackgroundIdiopathic/isolated REM sleep behavior disorder (iRBD) is widely regarded as an early sign of neurodegeneration leading to synucleinopathies. While circadian rhythm alterations in iRBD have been preliminarily demonstrated, evidence on melatonin secretion patterns in this clinical condition is limited. To address this knowledge gap, this exploratory study aimed to integrate salivary melatonin measurement with actigraphic monitoring in individuals with iRBD and age-matched healthy controls (HC) under real-life conditions. MethodsParticipants diagnosed with iRBD and HC underwent clinical evaluation and wore an actigraph for seven days and nights. Salivary melatonin concentrations were measured at five time points during the last night of recording. Comparative analyses were conducted on clinical data, actigraphic parameters, and melatonin levels between the two groups. ResultsiRBD participants (n = 18) showed greater motor (p < 0.01) and non-motor symptoms (p < 0.001), alongside disruptions in circadian sleep-wake rhythm compared to HC (n = 10). Specifically, actigraphy revealed a delayed central phase measurement (p < 0.05), reduced activity during the most active hours (p < 0.001), and decreased relative amplitude (p < 0.05). Total salivary melatonin concentration was significantly lower in iRBD (p < 0.05), with a slight but non-significant phase delay in dim light melatonin onset. ConclusionsThis exploratory study highlights a dysregulation of circadian sleep-wake rhythm coupled with reduced melatonin secretion in iRBD. Future research could add to these preliminary findings to evaluate novel treatment approaches to regulate the sleep-wake cycle and elucidate the implications of circadian dysregulation in the conversion from iRBD to neurodegeneration.

Assessing the Feasibility and Efficacy of Pre-Sleep Dim Light Therapy for Adults with Insomnia: A Pilot Study.

Background: Insomnia is increasingly recognized for its marked impact on public health and is often associated with various adverse health outcomes, including cardiovascular diseases and mental health disorders. The aim of this study was to investigate the efficacy of pre-sleep dim light therapy (LT) as a non-pharmacological intervention for insomnia in adults, assessing its influence on sleep parameters and circadian rhythms. Methods: A randomized, open-label, two-arm clinical trial was conducted over two weeks with 40 participants aged 20-60 years, all of whom had sleep disorders (CRIS, KCT0008501). They were allocated into control and LT groups. The LT group received exposure to warm-colored light, minimizing the blue spectrum, before bedtime. The study combined subjective evaluation via validated, sleep-related questionnaires, objective sleep assessments via actigraphy, and molecular analyses of circadian clock gene expression in peripheral blood mononuclear cells. Baseline characteristics between the two groups were compared using an independent t-test for continuous variables and the chi-squared test for categorical variables. Within-group differences were assessed using the paired t-test. Changes between groups were analyzed using linear regression, adjusting for each baseline value and body mass index. The patterns of changes in sleep parameters were calculated using a linear mixed model. Results: The LT group exhibited significant improvements in sleep quality (difference in difference [95% CI]; -2.00 [-3.58, -0.43], and sleep efficiency (LT: 84.98 vs. control: 82.11, p = 0.032), and an advanced Dim Light Melatonin Onset compared to the control group (approximately 30 min). Molecular analysis indicated a significant reduction in CRY1 gene expression after LT, suggesting an influence on circadian signals for sleep regulation. Conclusions: This study provides evidence for the efficacy of LT in improving sleep quality and circadian rhythm alignment in adults with insomnia. Despite limitations, such as a small sample size and short study duration, the results underscore the potential of LT as a viable non-pharmacological approach for insomnia. Future research should expand on these results with larger and more diverse cohorts followed over a longer period to validate and further elucidate the value of LT in sleep medicine. Trial registration: The trial was registered with the Clinical Research Information Service (KCT0008501).

Role of Dinacharya in Eye Care - A Preventive Approach

Ayurveda, the traditional system of medicine indeed emphasizes a holistic approach to health that includes both prevention and cure. In ophthalmology, the preventive measures play a crucial role in maintaining eye health and reducing the risk of developing various eye diseases that can lead to vision impairment. The ability to see clearly and perceive the world around us is crucial for various activities, from navigating our environment to appreciating the beauty of the world. The term Swasthavritta refers to a lifestyle or regimen followed to maintain health and prevent the occurrence of diseases. It encompasses a set of practices and principles that are aimed at promoting overall well-being and preventing imbalances in the body and mind. All the Acharyas have mentioned the mandatory practice of Dinacharya for health maintenance. Hence here an attempt has been made to compile the procedures to improve the health of the eye through which the various diseases of eye can be prevented. The daily regimens like Anjana, Nasya, Dhumapana, Kavala, Gandusha, Abyanga, Padatra dharana, Snana etc are included for maintaining eye health. The eyes should be always protected from the external factors like dust, heat, radiations, excessive screen time, over straining the eyes like in dim light reading , gadget use in dim light etc. Most of the Netraroga in the current scenario can be prevented by healthy Dinacharya and by avoiding negligence.

Key HPI axis receptors facilitate light adaptive behavior in larval zebrafish

The vertebrate stress response (SR) is mediated by the hypothalamic–pituitary–adrenal (HPA) axis and contributes to generating context appropriate physiological and behavioral changes. Although the HPA axis plays vital roles both in stressful and basal conditions, research has focused on the response under stress. To understand broader roles of the HPA axis in a changing environment, we characterized an adaptive behavior of larval zebrafish during ambient illumination changes. Genetic abrogation of glucocorticoid receptor (nr3c1) decreased basal locomotor activity in light and darkness. Some key HPI axis receptors (mc2r [ACTH receptor], nr3c1), but not nr3c2 (mineralocorticoid receptor), were required to adapt to light more efficiently but became dispensable when longer illumination was provided. Such light adaptation was more efficient in dimmer light. Our findings show that the HPI axis contributes to the SR, facilitating the phasic response and maintaining an adapted basal state, and that certain adaptations occur without HPI axis activity.