Different Regions Of Serbia Research Articles

Overview of the Implementation of the First Year of Immunization against Human Papillomavirus across Different Administrative Units in Serbia and Montenegro.

Despite the availability of a safe and effective vaccination, uptake of human papillomavirus (HPV) vaccination remains low worldwide. We aimed to analyze the coverage of HPV immunization during the first year of the immunization program and the sociodemographic characteristics across different administrative units in Serbia and Montenegro. Coverage of HPV vaccination in Serbia for females aged 9-14 and 15-19 years was 5.5% and 5.9%, respectively. The coverage rate of immunization against HPV in Montenegro for girls aged 9-14 years was 22.1%. Within Serbia, only one administrative region (Moravica) had HPV immunization coverage in girls 9-19 years old above 10%, 11 districts had coverage from 5 to 10%, while 13 districts had coverage below 5%. As per Montenegro, two administrative units, Cetinje and Berane, reported the highest coverage, with 39% and 36.4% of vaccinated eligible girls, respectively. When we explored the coverage of HPV immunization among girls aged 9-19 years across different regions in Serbia, we observed that the level of coverage did not correlate with the number of pediatricians or with the population density. In Montenegro, we observed a similar situation. On the other hand, we noticed a statistically significant moderate negative correlation (r = -0.446; p = 0.026) between HPV immunization coverage and the percentage of illiterate women in the administrative units. Comparing the coverage between the two countries we found that the higher coverage in Montenegro corresponded with a smaller number of female populations aged 9-14 years, with higher average net monthly income, with smaller population density and smaller number of pediatricians, among divorced persons, and among those without formal education or incompletely primary education. Taking into account the experiences in Montenegro, increasing immunization coverage in Serbia could be achieved through a more vigorous educational campaign targeting schools, the general population, and healthcare workers as well as by additionally incentivizing those engaged in these activities.

The incidence of patients with pseudoexfoliation in two different regions of Serbia

Introduction/Objective. Pseudoexfoliation (PEX) is an age-related systemic disorder, which can affect the whole body, as well as the eye. It is characterized by abnormal production and accumulation of pseudoexfoliative material. When present in the eye, it can cause different difficulties, but most common are PEX glaucoma (XFG) and intraoperative and postoperative complications of cataract surgeries. The aim of this study was to determine an incidence of patients with PEX in two different regions of Serbia. Methods. The study included 7451 patients scheduled for cataract surgery in two regions of Serbia. It was designed as a multicentric, retrospective study with evaluation of the medical records of all patients who underwent cataract surgery. The study evaluated: incidence of PEX syndrome and PEX glaucoma, age, and sex of patients, as well as preoperative antiglaucomatous therapy and intraoperative and postoperative cataract surgery complications. Results. PEX syndrome (XFS)was recorded in 676 patients (407 females and 269 males), while 243 patients had XFS. It represented 3.26% of patients included in the study. Mean age of XFG patients was 78.1 ?} 2.1 years with a statistically significant difference (p < 0.05) among incidence of XFG in females compared to males. Intraoperative and postoperative complications during cataract surgery were significantly common in patients with PEX (p < 0.05) Conclusion. PEX can complicate cataract surgery, while on the other hand XFG is more difficult to treat and control than most other glaucomas. Therefore, patients with PEX require special treatment during follow-up and treatment.

TRADITION AND CULTURE AS THE BASE FOR THE TOURISM PRODUCT: CASE OF UNESCO INTANGIBLE HERITAGE

The traditional Serbian folk dance Kolo was included in the UNESCO Representative List of the Intangible Cultural Heritage of Humanity in 2017. Kolo is a communal folk dance that is performed at both public and private celebrations of the most significant occasions in people's lives and the lives of communities. Kolo is an important socially integrating component of Serbian cultural heritage. The paper investigates the potential of learning Serbian dance as part of the cultural tourism offer of different regions in Serbia. Data were obtained through a survey using the Likert scale and analysis using SmartPls software. The aim of this research is to examine the tradition of local tourism based on folklore culture with which tourists have cultural contact as well as the effect of culture and tradition on the intention and interest in folklore culture. The study reveals a high and statistically significant positive impact of motivation for travel, culture, tradition and interest in Serbian traditional dance - kolo.

Building an infodemic management network in Serbia: Insights from public health practitioners

Abstract Background Vaccine coverage against COVID-19 in Serbia remains suboptimal, although COVID-19 vaccines became available to the public early during the pandemic. The presence of the infodemic, an overabundance of false, harmful, or outdated information, is one of the root causes of stagnation in vaccine demand. The Laboratory for infodemiology and infodemic management has been established at the Faculty of Medicine, University of Belgrade, to coordinate the efforts and mitigate the harmful impact of the infodemic. Methods Training for public health (PH) professionals, civil society organizations (CSOs), and academia was conducted in three Institutes of Public Health in different regions of Serbia. The training included an introduction to infodemic management and social listening, followed by group discussions. A questionnaire was given to all participants to share their experience about the infodemic sources, effects, and needs of the populations they serve. Results A total of 36 PH professionals, CSO representatives, and academics filled out the questionnaire. Over half of them (52.8%) had a primary affiliation in a public health institution and over 15 years of work experience. Although 83.3% of the participants stated that the infodemic presents a major public health issue in Serbia, only 16.7% were satisfied with current infodemic management activities in Serbia. Participants identify family members, doctors, and personal reasoning as the top three factors influencing health behavior. Participants identify the lack of institutional trust as the most important problem in infodemic management. Conclusions Insights from public health professionals, CSOs, and academia point to infodemic management being one of the main priorities in future public health activities. A stronger collaboration of experts of different backgrounds is needed to mitigate the complex nature of the infodemic. Key messages • Public health practitioners recognize the importance of the infodemic and search for adequate response. • The lack of institutional trust is one of the main obstacles to effective infodemic management.

The improvement of the body and udder hygiene influence on the milk safety and composition on small dairy farms in Serbia

The hygienic condition of dairy cows and their udders in 128 small household farms in different regions of Serbia, producing mostly milk, having 5-15 cows, a milk cooler and at least one milking machine, was evaluated by the following indicators: 1. general assessment of body cleanliness, 2. visual inspection of teats and udder base, and 3. maintenance of udder cleanliness, rated on a scale of 0 and 1 or 1 to 5. The samples were taken at each visit to the farm after cooling and tested for milk protein and fat content, somatic cell count (SCC) and the total number of microorganisms (TNM). The milk fat content was determined by the Gerber method, the protein content by the Kjeldahl method, the TNM according to the ISO 4833-1:2013 method and the SCC using Fossomatic TM. The IBM SPSS program was used for statistical data processing. Capacity, housing system, breed, milking system, number of cows, and capacity occupancy mostly had a very significant or significant impact on hygiene parameters, protein and fat content, SCC and TNM in milk. The hygiene parameters showed a continuous trend of improvement in relation to the visits. TNM mean scores increased after each visit, with significant differences between visits (F=9.63, P<0.0001). SCC scores varied very significantly between visits (F=5.17, P<0.0001). The number of visits tended to show a significant influence on the milk fat rate (F=2.221; P?0.1), but no influence on the milk protein rate (P=0.480; F=0.901).

West Nile virus in the Republic of Serbia-Diagnostic performance of five serological tests in dog and horse sera.

West Nile virus (WNV) is a zoonotic mosquito-borne virus classified as family Flaviviridae and genus Flavivirus. The first WNV outbreak in humans in the Republic of Serbia was recorded in 2012. Equids and dogs can show clinical symptoms after WNV infection and are often used as sentinels. This study aimed to (i) give insight into seropositivity for WNV in clinically healthy dog and horse sera in different regions of Serbia and (ii) compare diagnostic value of 'in-house' and commercially available indirect immunofluorescence (IFA) and enzyme-linked immunoassay (ELISA) tests to 'gold standard' virus neutralization test (VNT). Due to cross-reactivity, sera were tested for Usutu virus and tick-borne encephalitis virus in VNT based on the epidemiological data of field presence. Blood sera of dogs (n=184) and horses (n=232) were collected from 2011 to 2013. The seropositivity was confirmed by VNT in 36.9 % tested dog sera and 34.9% tested horse sera with highest positivity in regions near two big rivers, while in four dog and seven horse sera, positivity resulted from Usutu virus infection. Comparative results of diagnostic tests in dogs ranged from 18.7 % seropositivity by 'in-house' ELISA to 31.9% by commercially available ELISA. In horses, seropositivity ranged from 36.2% by 'in-house' IFA to 32.5% by commercially available IFA and from 26.3% by 'in-house' IgG ELISA to 20.9% by commercially available ELISA. There were no statistically significant differences according to the McNemar test between 'in-house' and commercially available IFA and ELISA test in horse sera, while the same was not true for two ELISAs used in dog sera (χ2 =8.647, p=.003). Established seropositivity in dogs and horses was in accordance with the epidemiological situation and WNV spread in the Republic of Serbia and proven Usutu virus co-circulation. 'In-house' tests remain a valuable tool in early diagnostics of WNV.

Izazovi obrazovno-vaspitnog procesa u "novoj normalnosti" - perspektiva nastavnika osnovnih škola

In the situation of the pandemic teachers face multiple difficulties in terms of achieving the educational function of the school. In order to understand the challenges faced by primary school teachers when organizing the educational process under changed conditions, a qualitative research was conducted in which 12 representatives of six primary schools from different regions of Serbia were interviewed using the semi-structured interview method. The obtained results point to the initial challenges of teachers in terms of using digital technologies and online tools and ensuring "equal education" for all students, dealing with negative changes in student behaviour patterns, a lack of space for the implementation of the school's preventive role in terms of risky student behaviour, as well as to teachers' needs for technical and professional support in organizing preventive work with students in current conditions. Taking into account all the limitations of the research, it can serve as a general indicator of the current needs of teachers for support in pandemic education, but also as a starting point for further research.

HONEYBEE VIRUSES PRESENCE IN SERBIAN APIARIES: A REVIEW

The honey bee Apis mellifera is an important beneficial insect recognized by production of honeybee products, having an important role in agricultural production through pollination, and play an equally crucial role in conserving the biodiversity in many natural ecosystems. According to available literature data, in the last more than 20 years, dramatic honey bee winter colony losses have been reported frequently all over the world that could have a direct influence on human food resources and can affect not only apiculture or agriculture, but also has an anthropological threats. One among many reasons for global bees-decline phenomenon is the influence of many viruses on honeybees’ health. Until today, in total, 36 viruses is detected in honey bees, and for many of them still without known pathogenicity on honeybees’ health. However, it is well known that some of these viruses like Acute Bee Paralysis Virus (ABPV), Chronic Bee Paralysis Virus (CBPV), Deformed Wing Virus (DWV), Black Queen Cell Virus (BQCV), Sacbrood Virus (SBV), Kashmir Bee Virus (KBV), Israeli Acute Paralysis Virus (IAPV), Slow bee paralysis virus (SBPV), Varroa destructor virus (VDV1) and some others has direct or indirect influence on individual honeybee or on whole honeybees colony health. In this paper the review of existing literature data on the presence, prevalence and characterization of honeybee viruses detected in honeybee colonies and apiaries from different regions in Serbia from first detection of their presence in 1986 till nowadays is presented and discussed.

The Development of Organic Agriculture in Serbia and Worldwide

Summary Organic agriculture is a food production system that sustains the health of people, soils and ecosystems with no adverse consequences, combining tradition, innovation and science. The development of such type of agricultural production, from its beginnings to the present day, has encompassed a number of specific stages both in Serbia and worldwide. Accordingly, the purpose of this survey study is to present the developmental course of organic agricultural production in Serbia and worldwide. The paper also summarises the state of organic plant production across different regions of Serbia, arguing that the Serbian organic production has been increasing since the 1990s. Following the political changes in Serbia in 2000, foreign donations, investments and organisations have significantly enhanced the country’s organic production sector as a whole. The largest number of organic producers (1/3) and the largest areas devoted to organic farming are concentrated in Vojvodina.

CARP EDEMA VIRUS DISEASE IN SERBIA - DISEASE OUT OF CONTROL

A poxvirus named carp edema virus (CEV), is the causative agent of carp edema virus disease (CEVD), which is an emerging disease of global concern that may cause high rates of morbidity and mortality in ornamental koi and common carp and. Common carp (Cyprinus carpio) is the most important fish species for warm‐water aquaculture in Serbia. CEVD was first time detected in Serbia in 2017. During 2017-2020 period, increasing number of CEVD outbreaks in carp farms is reported. Carp were collected from farms in different regions of Serbia from 2017 to 2020. The fish were sampled for disease diagnosis because they exhibited lethargy and anorexia, which eventually led to mortality. Mortality started with clinical signs of hypoxia while fish swam slowly without escaping reactions. The gills were pale and covered with a thick mucus layer. In advanced cases, the lesions in the gills turned into a necrotizing form. A moderate to high amount of opportunistic freshwater bacteria were isolated from gills of diseased fish. By performing real-time PCR, CEV was detected in 38 samples of diseased carps from 21 carp farms. These outbreaks further confirm the spread of CEVD and the need for practitioners to be vigilant for outbreaks of this disease. To prevent further spreading of the disease, it is very important to introduce CEV testing before fish movement. To avoid further transmission of the virus to common carp populations in Serbia, testing of CEV should become part of fish disease surveillance programs. Fish health service should be aware of the presence of CEV in Serbia which may result in high losses in carp aquaculture. Action should be taken also to prevent transmission of CEV to carp populations in open waters.

Variability of genetic correlations of milk yield and fertility traits in Simmental cows in different regions of Serbia

The Simmental breed of cattle is mostly reared in the central part of the Republic of Serbia, where it makes up about 80% of all breeds. In areas of more intensive cattle production, populations of cattle with pronounced milk yield are reared. In more extensive, as well as hilly and mountainous areas, somewhat less productive animals are raised. The main goal of this study was to examine the variability of genetic correlations of milk and fertility traits on the farms of individual agricultural producers using modern methods, depending on the breeding area, i.e. the region in which they are bred and reared. This study included 2589 controlled Simmental heifers, with lactations concluded during one year. All first calving heifers were housed and reared on agricultural family farms in the area of Central Serbia. The paper examines genetic correlations between the following traits of milk yield and fertility: duration of lactation, milk yield in standard lactation, milk fat content in standard lactation, milk fat yield in standard lactation, yield of 4% FCM in standard lactation, age at first calving, duration of service period. The results of the study of genetic correlations were obtained using mixed LSMLMW models (Harvey 1990). The examined genetic correlations of milk yield and fertility traits in Simmental cows showed pronounced variability depending on the breeding area where the cows are reared.

Weather suitability for outdoor tourism in three European regions in first decades of the twenty-first century

Outdoor tourism and recreational activities strongly depend on actual meteorological conditions. Traditionally, in three studied regions, the peak of tourists’ streams concentrates in summer months. In the present study, we assess suitability of weather conditions for various forms of outdoor tourism in different regions of Serbia, Poland and Ukraine. Additionally, how the location of the station differentiates temporal patterns of weather suitability will be discussed. To analyse the suitability of weather conditions for various forms of outdoor recreation, we have chosen 23 meteorological stations of the national weather networks which represent different tourism areas and destinations. For each weather station, daily data for the period 2000–2017 of air temperature, relative humidity, total cloud cover and wind speed (at 10 m above ground) for 12 UTC as well as the daily maximum and minimum temperature, precipitation totals and snow cover depth were applied. Suitability of climate for outdoor recreation and tourism is assessed by the Weather Suitability Index (WSI) based on Błażejczyk’s bio-thermal weather classification. The results of research show that passive forms of recreation (sun and air bathing) are preferred mostly in months from May till August or September. For the active forms of recreation, weather in summer months is very oppressive, especially in the resorts located in the south (Serbia, southern Ukraine). Active forms of recreation are preferred there in autumn, winter and spring months.

Convergence clubs in different regions of Serbia

ABSTRACT This paper investigates the existence of club convergence on the NUTS (Nomenclaturedes Unités Territoriales Statistiques) 3 level in Serbia. While a common approach in investigating convergence is based on dividing units of observation a priori into individual groups based on some of their particular characteristics, we use a method developed by Phillips and Sul that allows identification of clusters of convergence on the basis of an algorithm that is data-driven and thereby avoids a priori classification of the data into subgroups. We use data on real gross valued added (GVA) per capita for the NUTS3 level in Serbia for the period 2001–2017. Our results show that there are two convergence clubs in Serbia, while the Belgrade district shows no signs of convergence with any of the other clubs.

Phenolic Composition Influences the Health-Promoting Potential of Bee-Pollen

Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.

Comparative study of the chemical composition and biological potential of honey from different regions of Serbia

A chemical characterization and biological activity of 7 honey types (multifloral, linden, rapeseed, sunflower, phacelia, acacia and honeydew honey) from different regions of Serbia were presented. The physicochemical characteristics, mineral content, antioxidant and antimicrobial activity were estimated and discussed. All honeys showed good nutritional characteristics, according to the adopted criteria of the standard codex for honey. The concentrations of 15 elements in honey samples were determined using inductively coupled plasma optical emission spectroscopy (ICP-OES). Limits of quantification were in the range from 0.003 mg/kg (Cd, Cr, Cu, Mn, and Pb) to 1.50 mg/kg for sodium. Precision was expressed as relative standard deviation and its values were lower than 1.50%. Accuracy was evaluated with certified reference material (fish protein, DORM 4, NRC and cooking chocolate, SRM 2384, NIST) and obtained recovery percentages were 71-127%. The content of the most abundant elements (K, Mg, and Na) and microelements (Al, Cu, Fe, Mn, Ni, Se, Si, and Zn) showed a significant variability in accordance of their botanical and geographical origin, but also the influence of harvest conditions. The levels of toxic metals (As, Cd, Cr, and Pb) are much lower than the maximal allowed for these metals according to European Commission Regulation. The antioxidant activity was evaluated by DPPH (Diphenylpicrylhydrazyl) assay while antimicrobial activity against Gram positive and Gram negative bacteria (E. coli and S. aureus) and fungi Candidi albicans was estimated by microdilution assay. The free radical scavenging assay (%RSA) varies significantly among the honey samples. The highest antioxidant activity was observed with honeydew honeys (over 75% RSA) and the lowest was observed in the acacia, ranging from 22.96 to 24.57% RSA. All honeys tested in this study exhibited antibacterial activity, with inhibition of bacterial growth, generally the higher against E. coli (80% for linden honeys) than against S. aureus (mostly much lower than 50%). Activity against Candidi albicans was almost negligible. The dataset composed of all experimental results was subjected to a chemometric evaluation and cluster analysis as hierarchical nonsupervised method was applied. According to the obtained outcome of applied algorithms, both botanical and geographical origin have important role in the elemental composition, antioxidant and antimicrobial activities.

Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia

The present study provides insight into the diversity of 147 Xanthomonas campestris pv. campestris (Xcc) isolates obtained from six Brassica oleracea vegetable crops (broccoli, cabbage, cauliflower, collard greens, kale, kohlrabi) and the winter oilseed rape crop Brassica napus, collected from different regions in Serbia in 2014. The XCF/XCR pathovar‐specific primer set was used for fast preliminary identification. In repetitive sequence‐based PCR (BOX, ERIC and REP) of all isolates, a higher level of genetic diversity was found in winter oilseed rape isolates compared to isolates from the other hosts. ERIC and REP‐PCR showed the highest heterogeneity, with 10 and nine banding patterns, respectively. The REP‐PCR results showed the highest correlation (70%) with those obtained with multilocus sequence analysis (MLSA), performed with 10 housekeeping genes (fusA, gap‐1, gltA, gyrB1, lacF, lepA, rpoD, dnaK, fyuA and gyrB2). Three distinct phylogenetic groups of winter oilseed rape isolates were detected using MLSA. Two genes, gltA and rpoD, showed the greatest ability to identify and discriminate winter oilseed rape Xcc isolates from isolates of the other six hosts. The lepA gene exhibited specific three‐nucleotide changes in sequences of some of the isolates. Results of virulence testing of 18 representative isolates showed statistically significant host–pathogen specialization for Xcc isolates from winter oilseed rape, cauliflower, kale and kohlrabi. In conclusion, oilseed rape isolates are more genetically diverse and show greater specialization to their host in comparison to the rest of the tested isolates from other brassica hosts.

Phenotypic correlation of traits of production and reproduction of Simmental cows in different regions of Serbia

The main goal of this research was to examine, using modern methods, the variability of phenotypic correlations of production performances and reproductive properties of Simmental cows reared on the farms of individual agricultural producers, in different breeding areas of the Republic of Serbia. The study of phenotypic correlations of milk performance and fertility properties in different regions of Serbia was carried out on a total of 3.056 primi parous Simmental heifers under control, with lactations completed within one year. The examined animals were reared on different individual farms, and mainly in very different conditions of housing and nutrition, depending on the breeding area. The study of phenotypic correlations included the following milk performance traits: milk yield, milk fat content, milk fat yield, yield of 4% corrected milk; and fertility properties: age at first calving and service period. The results of the study among other things indicate that although the phenotypic correlations between the fertility and milk performance properties show different degrees of variation, they should be taken into account in the final assessment of the breeding value of the animal, so that the breeding selection programs are more comprehensively designes.

Assessment of spatial and temporal variations in trace element concentrations using honeybees (Apis mellifera) as bioindicators

With the increase in anthropogenic activities metal pollution is also increased and needs to be closely monitored. In this study honeybees were used as bioindicators to monitor metal pollution. Metal pollution in honeybees represents pollution present in air, water and soil. Concentrations of As, Cs, Hg, Mo, Sb, Se, U and V were measured. The aim of this study was to assess spatial and temporal variations of metal concentrations in honeybees. Samples of honeybees were taken at five different regions in Serbia (Belgrade - BG, Pančevo - PA, Pavliš - PV, Mesić - MS, and Kostolac - TPP) during 2014. Spatial variations were observed for Sb, which had higher concentrations in BG compared to all other regions, and for U, with higher concentrations in the TPP region. High concentrations of Sb in BG were attributed to intense traffic, while higher U concentrations in the TPP region are due to the vicinity of coal fired power plants. In order to assess temporal variations at two locations (PA and PV) samples were taken during July and September of 2014 and June, July, August and September of 2015. During 2014 observing months of sampling higher concentrations in July were detected for Sb and U in BG, which is attributed to lifecycle of plants and honeybees. During the same year higher concentrations in September were observed for As, Sb in PA and Hg in PV. This is due to high precipitation during the peak of bee activity in spring/summer of 2014. No differences between months of sampling were detected during 2015. Between 2014 and 2015 statistically significant differences were observed for Hg, Mo and V; all elements had higher concentrations in 2014. This is in accordance with the trend of reduction of metal concentrations in the bodies of honeybees throughout the years in this region.

Agro-morphological characterisation and evaluation of a Serbian vineyard peach [Prunus persica (L.) Batsch] germplasm collection

Seventy-five vineyard peach [Prunus persica (L.) Batsch] accessions, obtained by seed propagation and originating from different regions of Serbia, were characterized using a set of 39 agro-morphological traits. The evaluation was carried out over a 3-year period in a vineyard peach collection situated at the Radmilovac Experimental Station, owned by the Faculty of Agriculture, Belgrade University. To investigate diversity, descriptive statistics and multivariate analyses were used. Most studied traits showed high variability. The variability of descriptive traits was highly pronounced for fruit ground and flesh colour; regarding metric traits, the highest variation was recorded for trunk circumference, petal width, flower density, fruit set, fruit weight, sweetness index, fruit taste and fruit flavour. According to PCA, traits with high discriminating power were those related to flower type, colour and size, fruit size and presence of over colour, tree height, ripening time and fruit taste. The cluster analysis grouped accessions into three main clusters and a few sub-clusters. Agronomically-important traits allowed a clear separation between the clusters, while morphological traits had almost no impact on this. This study provided information that is important for assessing the biodiversity of vineyard peach accessions, to develop guidelines useful for the description, classification and conservation of valuable local germplasm. Moreover, the phenotypic variability in the vineyard peach collection was found to be very high, suggesting there is extensive genetic diversity available for peach cultivar and rootstock breeding programs.

Aflatoxin M1 in processed milk: Occurrence and seasonal variation with an emphasis on risk assessment of human exposure in Serbia

The objectives of this study were to assess aflatoxin M1 (AFM1) contamination in processed milk and dairy products, and to estimate the mean daily exposure of the adult Serbian population to AFM1 due to milk consumption. A total of 1734 samples, comprising heat treated cow’s milk (n=1233), infant formulae (n=349), milk powder (n=94) and dairy drink (n=58), were analyzed for AFM1 presence using an Enzyme-Linked Immunosorbent Assay (ELISA) commercial kit. Samples were collected from different regions of Serbia during four seasons each year during 2015 and 2016. The incidences of AFM1 contamination were 77.8% with a mean level of 0.027±0.03 μg/L (range of <0.005-0.278 μg/L) in samples collected in 2015, and 98.4% with a mean level of 0.039±0.02 μg/L (range of <0.005-0.28 μg/L) in samples collected in 2016. The highest AFM1 levels were measured in October 2015 (0.278 μg/L) and September 2016 (0.279 μg/L). Based on EU regulation, 214 (17.3%) milk samples exceeded the maximum residue limit (0.05 μg/L). The estimated daily intake (EDI) of AFM1 during different seasons of year for males and females was in the range of 0.022-0.330 (mean 0.20) ng/kg/bw/day and 0.022-0.30 (mean 0.18) ng/kg/bw/day, respectively. The calculated EDI indicate a public health concern due to the carcinogenic effects of AFM1.