Different Provinces In China Research Articles

Convergence effect of the Belt and Road Initiative on income disparity: evidence from China

The international economic effects of the Belt and Road Initiative (BRI) have received much attention, but few studies have focused on the impact of the BRI on domestic regional income disparities. Here, a theoretical framework is proposed based on the logic of public policy analysis in economic geography aiming at studying the impact of the BRI on the convergence of inter-city income disparities in China. Specifically, taking the BRI as a quasi-natural experiment, the impact of the BRI on the convergence of inter-city income disparities in 26 provinces of China is studied empirically using the difference-in-differences method. We find that the BRI has indeed contributed to the convergence of regional income disparities, and this convergence effect is continuously dynamic in its nature. The effects of trade opening and industrial structure transformation are the paths through which the BRI contributes to the convergence of income disparities. Furthermore, we find that there is significant heterogeneity in the effects of the BRI on the convergence of income disparities among cities in different provinces in China. The convergence effect of the BRI on the income disparities among cities in East China is small and insignificant, whereas it can significantly reduce the income disparities among cities in Central and West China. The research in this article has important application value for exploration of the regional income distribution effect of the BRI.

Evidence for the Presence of Hyphae and Fruiting Body Calcium Oxalate Crystallites in the Split Gill Medicinal Mushroom Schizophyllum commune (Agaricomycetes).

Biomineralization is the phenomenon by which organisms form crystals. Studies have shown that many fungi can biomineralize, producing calcium oxalate crystals on their hyphae and fruiting body. Schizophyllum commune is a medicinal and edible fungus found worldwide, however, studies on biomineralization in this fungus are limited. Here, samples of Sch. commune fruiting bodies were collected from three different provinces in China and hyphal cells were cultured. Using light microscopy, FE-SEM, and EDAX, we identified crystals on the fruiting body and mycelium of each strain and analyzed their morphological characteristics and ion content. These data demonstrate that biomineralization occurs in Sch. commune in nature as well as during subsequent in vitro culture.

Spatial variation and driving mechanism of polycyclic aromatic hydrocarbons (PAHs) emissions from vehicles in China

Rapid motorization has made vehicles become one of the major sources of air pollution and poses substantial risk to human health. Accurate estimation of spatiotemporal variation and driving factors of vehicle emissions will be valuable for pollution control and public health protection. Here, taking polycyclic aromatic hydrocarbons (PAHs) as an example, a dynamic vehicle emission model (DVEM) was developed to assess vehicle emissions. The inventories of vehicle emissions from 2002 to 2017 were estimated under different emission standards and vehicle kilometers traveled for all types of vehicles in China. Vehicle PAHs emissions peaked 1586.85 tonne (t) in 2012 and declined by 36% in the follow-up five years because the vehicle growth has been offset by the upgraded emissions standards. There were remarkable variations among different provinces in China. The vehicle emissions are higher in eastern coastal provinces like Guangdong, Shandong, Jiangsu, and Zhejiang, while lower in the western provinces except Xinjiang. Motorcycles (44.1%) and light duty vehicles (17.8%) were the main contributors to PAHs emissions. The higher urbanization within a region, the larger its vehicle emission density. Urban road density may be linked with the number of on-road vehicles, which is the real driving factor of emissions. Therefore, integrated management should be taken by government to reduce the impacts of vehicle PAHs emissions.

Analysis of Public Management Performance Evaluation Model Based on Fuzzy DEA Model

The evaluation of public management performance is the key to identify the effectiveness of the work of public administration, and public policy is an important guarantee for the orderly promotion of the work of public administration; therefore, it is important to analyze the effectiveness of public management policies to evaluate public management performance. Taking environmental protection policies as an example, this paper constructs an environmental‐economic DEA efficiency analysis model using the DEA method to conduct a comprehensive evaluation of EEE in different provinces of China. It is found that EEE is highly correlated with the regional economic development level and is highly influenced by policy fluctuations, but the overall EEE of different provinces from 2016 to 2020 tends to be consistent and has a certain degree of stability. The conclusions of this paper have important implications for policy guidance, emphasizing the need to focus on the coordination of economic development and environmental protection, and the need for the improvement of the environmental policy system to be continuously optimized in an incremental manner, taking into account the actual situation of local development.

Empirical Analysis of Economic Development Level of Chinese Provinces Based on PCA

In the past decades, China's overall economy has maintained growth, the economy of China's provinces in maintaining growth at the same time there are different degrees of change, and the development level of different provinces there are similarities and differences. Therefore, this paper takes the gross regional product (GDP) of 31 provinces including Beijing, Shanghai, Guangdong and Jiangsu from 2018 to 2020 as the dependent variable, uses principal component analysis (PCA) to comprehensively compare the development degree of 31 provinces, and evaluates and analyzes the results, so as to compare and find the similarities and differences of economic development of different provinces. In order to provide theoretical reference and guidance for national and regional development and policy formulation and implementation, the author compares the economic development gap among different provinces in China and reflects on the policy direction and path selection of China's acceleration of economic development in the context of the outbreak of COVID-19.

Development of a genome-wide 200K SNP array and its application for high-density genetic mapping and origin analysis of Camellia sinensis.

Development of a genome-wide 200K SNP array and its application for high-density genetic mapping and origin analysis of Camellia sinensis.

Analysis of Regional Water and Energy Consumption Considering Economic Development

The evaluation of regional water and energy consumption is of great significance to improving regional resource utilization. This paper analyzed the water and energy footprints in different provinces of China, considering regional economic levels. The results indicate: (1) both the largest water footprint and water footprint per capita were in Xinjiang and agriculture had the largest value; (2) Shandong was the largest energy consumer, Ningxia had the largest energy footprint per capita, and coal occupied the largest proportion for the top five energy footprint provinces; and (3) the resource input–output efficiencies in Beijing and Fujian were high, while water and energy consumption were low and gross regional product was high, compared with the average value of China. The situations in Xinjiang and Inner Mongolia were opposite. The change of consumption pattern for each inhabitant, the adoption of water-saving technology, and an increase to water-saving awareness would be helpful to decrease regional water consumption. An increase of regional energy use efficiency and a change to reduced energy consumption would contribute to the decreasing of regional energy consumption. More attention should be paid to renewable and clean Energies. In addition to solution from the local perspective, the virtual water trade and the energy product trade may relieve regional resource pressure in some extent, and the possible influencing should be considered at the same time. This paper could provide suggestions for regional resource utilization and sustainable development.

Effects of radio frequency heating on microbial populations and physicochemical properties of buckwheat

Microbial contamination is a persistent problem for grain industry. Many studies have shown that radio frequency (RF) heating can effectively reduce pathogens populations in low moisture foods, but there is a lack on the efficacy to decontaminate natural microbiome. The main objectives of this study were to investigate the effects of different RF heating conditions on natural microbial populations and physicochemical properties of buckwheat. In this study, 30 buckwheat samples collected from 10 different Provinces in China were analyzed for their microbial loads, and the samples with the highest microbial populations were used for further study to select the suitable RF heating conditions. The results showed that microbial loads in tested buckwheat kernels were in the range of 3.4–6.2 log CFU/g. Samples from Shanxi (SX-3) had significantly higher microbial counts than other samples. The selected four temperature-time combinations: 75 °C-20 min, 80 °C-10 min, 85 °C-5 min, and 90 °C-0 min of RF heating could reduce microbial counts to <3.0 log CFU/g in buckwheat kernels at 16.5% w.b. moisture content. Furthermore, the reduction populations of the inoculated pathogens (Salmonella Typhimurium, Escherichia coli, Cronobacter sakazakii, and Bacillus cereus) reached 4.0 log CFU/g under the above conditions, and almost 5.0 log CFU/g especially at high temperature-short holding time combinations (85 °C-5 min and 90 °C-0 min). Besides, physicochemical properties evaluation also showed the insignificant color changes and nutrients loss after RF treatment at 90 °C-0 min. Therefore, the RF heating at 90 °C-0 min holds greater potential than the other lower temperature-longer holding time combinations for applications in buckwheat pasteurization.

Advancing greenhouse gas emission factors for municipal wastewater treatment plants in China

Estimations of greenhouse gas (GHG) emissions from municipal wastewater treatment plants (MWTPs) remain significant uncertainties in China owing to a lack of reliable emission factors (EFs). This study developed a framework to obtain multi-level (technology, province, and nation) GHG EFs of MWTPs using a database containing 3107 MWTPs in China and published site-specific monitoring data. Results show that GHG EFs of different technologies range widely from 180.0 to 615.7 g CO2-eq/t wastewater, and significant differences are also observed among different provinces in China (190.5–600.3 g CO2-eq/t wastewater), which are generally lower than the previous estimates. It confirms the importance of more detailed technology classification and considering the technological disparity of different provinces in refining GHG estimations of MWTPs. To test the feasibility of the developed EFs, we compared GHG emissions from MWTPs based on multi-level EFs at different spatial and temporal scales. Similar estimation results imply that selecting corresponding EF depending on the availability of activity data would simplify GHG estimations of MWTPs without sacrificing much accuracy. This study contributes a set of well-developed EFs to improve the estimates of GHG emissions from MWTPs, and also offers a method to develop GHG EFs for other sectors.

Dynamic Relationship Between China’s Environmental Protection Investment and Regional, Environmental Pollution

The carrying capacity of China’s resources and environment has reached a limit. The economic development of different regions has been forced to abandon the original economic development mode manifesting high pollution, high energy consumption, and high emission and to step forward to the new economic development model promoting low energy consumption, low emission, and low pollution. Environmental issues are typical manifestations of market mechanism failure. Government investment in environmental protection, which effectively improves environmental quality, is necessary to achieve sustainable economic development. An index system of the influencing factors that affect regional environmental pollutant emissions was established first in this study to measure accurately the relationship between environmental protection investment in different provinces in China and regional environmental pollution. System GMM (Generalized Method of Moment) method was used to analyze the impact of environmental protection investment on pollutant emissions in 30 provinces in China from 2007 to 2016. Results show that the system GMM method can effectively solve variable endogeneity. Environmental protection investment of explanatory variables has a significant negative effect on pollutant emissions. Among the control variables, per capita GDP (Gross Domestic Product), industrial structure, resident consumption level, and technology market turnover have a significant inhibitory effect on pollutant emissions. Among the control variables, investment in fixed assets and import and export trade is vital in promoting pollutant emission growth. Conclusions provide a reference for improving the governance level of environmental protection investment in China’s provinces, controlling environmental pollution and ecological damage, and realizing a green economic development method.

Epidemiological Analysis From 2018 to 2020 in China and Prevention Strategy of Porcine Circovirus Type 2.

Porcine circovirus type 2 (PCV2) is one of the smallest known animal viruses and is the main pathogen of PCV-associated diseases (PCVAD). Epidemiological surveillance results have shown that the PCV2 infection rate is on the rise in China, thus, PCV2 disease prevention and control has become a huge challenge for the Chinese swine industry. We collected clinical samples from multiple different provinces in China from 2018 to 2020 and found that the positive rate of PCV2 was 53% (3619/6872), identity between the cloned 62 ORF2 genes was 84.4–100% and identity between the cloned 62 ORF2 sequences and reference sequence was 72.9–99.8%. Genetic evolution analysis found that PCV2d accounted for 79% (49/62 samples), PCV2a for 12.9% (8/62 samples), PCV2b for 8% (5/62 samples), and PCV2c and PCV2e genotypes were not found. However, most commercial PCV2 subunit vaccines are based on the PCV2a genotype, and there are very few vaccines based on PCV2b or PCV2d. Therefore, the homologous and heterologous protection ability of PCV2b and PCV2d Cap proteins based on the baculovirus against the PCV2b and PCV2d infections was evaluated, which is expected to design and develop excellent PCV2 protein vaccine candidates. This study found that both PCV2b and PCV2d Cap proteins can increase the level of humoral immunity and cellular immune response in mice. Importantly, both PCV2b and PCV2d cap proteins can provide homologous and heterologous protection against the PCV2b and PCV2d viruses. Overall, this study provides a reference for the prevention and control of PCVAD in mainland China and the development of PCV2 vaccines.

Simultaneous Determination of Purines and Uric Acid in Chinese Chicken Broth Using TFA/FA Hydrolysis Coupled with HPLC-VWD.

Chinese chicken broth is well known for its outstanding nutritional value and flavor, widely consumed in China. This study was designed to develop a sensitive and accurate high-performance liquid chromatography-variable wavelength detector (HPLC-VWD) method to simultaneously determine purines and uric acid in Chinese chicken broth for gout and hyperuricemia dietary management. Chromatographic separation was performed on an Agilent TC-C18 (2) column (4.6 mm × 250 mm, 5.0 µm), using 0.02 M KH2PO4 (pH 4.0) as a mobile phase. Sample pretreatment was optimized to enable the extraction of all analytes from Chinese chicken broth. The optimal pretreatment conditions were chicken broth-60% trifluoroacetic acid (TFA)/20% formic acid (FA) (1:1, v/v) in a volume ratio of 1:3 and hydrolysis for 40 min at 85 °C in a water bath. The limits of detection (LODs) and limits of quantification (LOQs) of the purines and uric acid were 0.58–1.71 µg/L and 1.92–5.70 µg/L, respectively. The recoveries were 91–101%, with the relative standard deviations (RSDs) lower than 3%. The complete method has been successfully applied to determine purines and uric acid in various Chinese chicken soups obtained from different provinces in China.

Genotype diversity of Mycoplasma Hyopneumoniae in Chinese swine herds based on multilocus sequence typing

BackgroundBetween 2018 and 2020, 989 clinical specimens from pigs showing clinical signs of a variety of swine diseases in 27 provinces in China were sampled and submitted for further testing. Nested PCR targeting the 16S rRNA gene of Mycoplasma hyopneumoniae and subsequent sequencing were used to analyse these specimens. Mycoplasma hyopneumoniae-positive samples were assayed by multilocus sequence typing (MLST). The aim of the study was to reveal the distribution of M. hyopneumoniae and determine the genotypes of M. hyopneumoniae in pig herds in China based on MLST.ResultsAmong these 989 samples, 199 samples were M. hyopneumoniae-positive. The M. hyopneumoniae positivity rate was 7.2% (35/494) in 2018, 18.4% (38/207) in 2019, and 43.8% (126/288) in 2020. In total, 47 samples were successfully assayed by MLST. Sixteen new M. hyopneumoniae sequence types from 9 provinces were recorded in the present study.ConclusionsThis is the first report on sample positivity rates and molecular typing results for M. hyopneumoniae in swine herds in China. MLST has revealed high genotype diversity among M. hyopneumoniae from different provinces of China.

Chinese Undergraduates’ Performance in HOTS and LOTS EFL Reading Comprehension for Different Reading Materials According to Gender

Many studies indicate that Chinese undergraduates face problems in their HOTS comprehension. The objective of this study is to investigate Chinese EFL undergraduates’ HOST and LOTS reading comprehension according to gender. A descriptive research design was employed in this quantitative study. The researchers used an English reading comprehension test involving 5 different materials (education, economy, entertainment, technology and natural disaster) as the research instrument. Each material consists of 3 LOTS questions and 2 HOTS questions. All the comprehension questions were multiple choice in nature. The sample of the study consists of 411 second year undergraduate students (261 females and 150 males) from a university in Hunan Province, China. SPSS Program for Windows version 25 was used to analyze the data (Independent samples t-test). The findings of this current study indicated that the female students performed significantly better than their male counterparts for both LOTS and HOTS comprehension questions. The female students also outperformed their male students in their overall score for comprehension. As such, these results clearly revealed that female undergraduates performed significantly better than the males in their EFL reading comprehension skills. However, this research only involves participants from one university; as a result, future research should include a larger sample from different provinces in China to get a better insight of students’ performance in EFL comprehension.

Isolation and pathogenic characterization of duck adenovirus 3 mutant circulating in China

Duck adenoviruses (DAdVs) include serotype 1 (DAdV-1) in the genus Atadenovirus and serotypes 2-4 (DAdV-2, 3, and 4) in the genus Aviadenovirus. DAdV-3 was initially isolated from Chinese Muscovy ducks in 2014, whereby the infected ducks exhibited yellowing and hemorrhaging in the liver, along with slight pericardial effusion, swelling, and hemorrhaging in the kidneys. In recent years, duck adenovirus infections have appeared in Muscovy duck farms in Fujian, Zhejiang, Anhui, Guangdong, and other places in China. They have an incidence rate of 40 to 55% and a mortality rate of 35 to 43%, resulting in great losses to the duck breeding industry. In this study, 7 DAdV-3 strains, designated as TZ193, FJPT20161124, GX20170519, FJZZ, GDMM, AHAQ, and GDHS were isolated from Muscovy ducks in different provinces of China during 2016–2019, and their complete genomics were sequenced. Their genomes all exhibited significant deletions in ORF67, which also had G to A transitions at the 41st and 977th nt positions, resulting in a stop codon. The pathogenicity of TZ193, a novel isolate of DAdV-3, was investigated in Muscovy ducks. TZ193 caused characteristic lesions of swelling as well as hemorrhagic liver and kidney in the infected ducklings. Moreover, the mortality rate of TZ193 in 5-day-old domestic ducks was 100%. Our data provide concrete evidence for the identification of the DAdV-3 novel variant mutant in China, which effects increased mortality in ducks. This highlights the necessity for monitoring the specific molecular epidemiology of novel DAdV-3 mutants and the development of new vaccines in the future.

Epidemiological and etiological aspects of enterovirus infection in Russia and Vietnam

The epidemic situation and etiological factors of enterovirus infection in Russia and Vietnam were analysed and compared. The identified strains of enteroviruses of 47 types, which circulated in Russia in 2018–2019, belonged to different species: Enterovirus species A (CV-A2, CV-A4, CV-A6, CV-A8, CV-A10, CV-A16, EV-A71), as well as Enterovirus species B and Enterovirus species C. The strains isolated from 87 children from southern Vietnam hospitalised in 2018–2019 into infectious hospitals while having enterovirus infection with exanthema were also studied. All identified strains were represented by Enterovirus species A: EV-A71 — 59 strains, CV-A10 — 20 strains, CV-A16 — 5 strains, CV-A6 — two strains and CV-A2 — one strain. Out of 59 viruses EV-A71, 53 strains belonged to genotype C4 and 6 strains belonged to genotype B5. The sequences of EV-A71 strains of genotype C4 from South Vietnam formed a monophyletic cluster with the sequences of EV-A71 viruses which circulated during 2016–2018 in different provinces of China, and they were very close to EV-A71 strains of the same genotype from the Yunnan Province. These strains were genetically different from Russian viruses and Vietnamese viruses identified in the years 2003–2005 and 2011– 2012. Most of the cases of enterovirus infection from southern Vietnam (78%) caused by EV-A71 virus of genotype C4 were reported in three provinces located in southern Vietnam in the Mekong Delta. The epidemic process and the etiology of enterovirus infection in Russia and Vietnam have common features. At the same time, the epidemic situation in these countries is not the same. The incidence of enterovirus infection is influenced by geographic, climatic, economic and demographic factors that differ in two countries. In the majority of territories of Russia, the climate is temperate or cold, seasonal rises in the incidence rates of enterovirus infection usually occur in the summer, when people go on vacation, spend a lot of time outdoors and swim in open reservoirs. In Vietnam, a constant high-level temperature, a high population density and a large proportion of children determine the higher incidence of enterovirus infection, especially in the southern provinces of Vietnam, compared to Russia. The fact that more than 20% of the Vietnamese population lives in the Mekong Delta, which is the largest river in Indochina, has a significant impact on the epidemic process of enterovirus infection in South Vietnam. The Mekong River which flows through China, Laos, Thailand, Cambodia and Vietnam, and carries huge streams of water, including rainstorm waters and sewages, from all of these countries into the southern provinces of Vietnam, which have the highest incidence rates of enterovirus infection in the country. The results of the research underline the importance of active epidemiological and virological surveillance of enterovirus infection, which plays the key role in informing the public health authorities about the changes in the epidemic situation in order to take appropriate measures and develop the prevention strategies. The goal of anti-epidemic and preventive measures is to reduce the incidence of enterovirus infection and the economic burden of this infection for Russia and Vietnam.

Surveillance and Reduction Control of Escherichia coli and Diarrheagenic E. coli During the Pig Slaughtering Process in China.

The microbial contamination of pork during the slaughter process, especially that of the hygiene indicator bacteria, Escherichia coli, is closely related to the safety and quality of the meat. Some diarrheagenic E. coli can cause serious foodborne diseases, and pose a significant threat to human life and health. In order to ascertain the current status of E. coli and diarrheagenic E. coli contamination during the pig slaughter process in China, we conducted thorough monitoring of large-sized slaughterhouses, as well as small- or medium-sized slaughterhouses, in different provinces of China from 2019 to 2020. The overall positive rate of E. coli on the pork surface after slaughter was very high (97.07%). Both the amount of E. coli contamination and the positive ratio of diarrheagenic E. coli in large-sized slaughterhouses (7.50–13.33 CFU/cm2, 3.44%) were lower than those in small- or medium-sized slaughterhouses (74.99–133.35 CFU/cm2, 5.71%). Combined with the current status of sanitary control in slaughterhouses, we determined that pre-cooling treatment significantly reduced E. coli and diarrheagenic E. coli in pork after slaughter, while microbiological testing reduced E. coli. Based on our monitoring data, China urgently needs to establish relevant standards to better control microbial contamination during pig slaughtering progress. This study provided a theoretical basis for the hygiene quality management of the pig slaughter industry in China.

Trends of morbidity and mortality of hand foot and mouth disease in China, 2008–2017

Objective To analyze the trends of morbidity and mortality of hand foot and mouth disease (HFMD) in China from 2008 to 2017, and provide scientific evidence for the development of HFMD prevention and control strategies. Methods The morbidity and mortality data of HFMD in China during this period were collected from China Public Health Science Data Center and the demographic data were collected from National Bureau of Statistics. Software Excel 2016 was used to establish the morbidity and mortality database of HFMD reports. Software JoinPoint 4.9.0.0 was used for JoinPoint regression analysis to estimate the annual average percentage change (AAPC) of reported morbidity and mortality of HFMD in different age groups and in different provinces in China from 2008 to 2017, and analyze the changing trend of HFMD morbidity and mortality. Results A total of 18 183 889 cases of HFMD were reported from January 2008 to December 2017, the average annual reported morbidity was 134.75/100 000. A total of 3 632 deaths were reported, with an annual average reported mortality of 0.027/100 000. The morbidity and mortality were stable from 2008 to 2017 (P>0.05), but the mortality showed a decreasing trend from 2010 to 2017 (APC: −22.98%, 95%CI: −29.64% to −15.70%, P 0.05). The mortality in age group 0–7 years remained stable (P>0.05). Conclusion The overall HFMD morbidity and mortality in China from 2008 to 2017 were relatively stable, but the mortality showed a decreasing trend from 2010 to 2017. The morbidity of HFMD showed increasing trend in Southern China, Southwestern China, Central China and Eastern China, but the morbidity of HFMD declined in northern China and northeastern China. The morbidity of HFMD was higher in children aged 0 to 7 years. It is suggested that HFMD prevention and control should be strengthened according to the change of HFMD epidemic in different areas to reduce the morbidity and mortality of HFMD.

Assessment of genetic diversity in chinese hulless barley accessions for qualitative traits

Cultivated barley (Hordeum vulgare L.) has been proven to be an economically important model plant and having large genetic diversity among the species. The effective exploitation of qualitative characters in barley can be measured by its genetic diversity and interrelationship. This study aims to determine the assessment of genetic diversity in Chinese hulless barley accessions for qualitative traits. Presently, in this study, the genetic diversity of 208 Chinese hulless barley from different Provinces of China, 111 genotypes were from the Tibet plateau, 30 Sichuan, 2 USA, 1 Canada, 12 Gansu, 51 Qinghai, 1 Yunnan was investigated; collected. Almost all the qualitative traits including crude protein, fiber, starch, neutral detergent fiber, and acid detergent fiber exhibited significantly high variability (p≤0.0001) among the cultivars. The data were analyzed using Statistics 8.1. In this study, significantly high variation was observed between starch content and neutral detergent fiber (23.64% and 11.54%). However, the highest diversity is based on the magnitude of the coefficient of variation exhibited in crude protein (13.82%), starch (12.87%), and fiber (12.17%). There was a significantly positive correlation between fiber, acid detergent fiber, and neutral detergent fiber except for starch content with crude protein and fiber that exhibited a significant negative correlation (r= -0.38*** and r= -0.92***). A large genetic diversity was observed through cluster analysis among all the 208 barley accessions, distance coefficient ranging between 0.28 and 75.86. The histogram revealed that frequency distributions of 208 different genotypes of hulless barley crop with all five different characters, crude protein, fiber, starch, neutral detergent fiber, and acid detergent fiber, showed normal distribution. It is concluded that this hulless barley study showed genetic diversity among the accessions and confirmed genetic diversity in various traits used.

Proteomic analysis of differentially expressed whey proteins in Saanen goat milk from different provinces in China using a data-independent acquisition technique

Whey proteins of Saanen goat milk samples from 3 provinces in China (Guangdong, GD; Inner Mongolia, IM; Shaanxi, SX) were characterized and compared using data-independent acquisition quantitative proteomics technique. A total of 550 proteins were quantified in all 3 samples. There were 44, 44, and 33 differentially expressed proteins (DEP) for GD versus IM, GD versus SX, and IM versus SX, respectively. Gene ontology annotation analysis showed that the largest number of DEP for the 3 comparisons were as follows: for biological processes: response to progesterone, glyceraldehyde-3-phosphate metabolic process, and negative regulation of megakaryocyte differentiation; for molecular functions: antioxidant activity, binding, and peroxiredoxin activity; and for cellular components: the same category of extracellular regions for the 3 comparisons, respectively. Pathways for the DEP of 3 comparisons were (1) disease; (2) synthesis and metabolism; and (3) synthesis, degradation, and metabolism. Protein-protein interaction network analysis showed that DEP for GD versus SX had the most interactions.