SummaryFollowing incubation of 59Fe with pooled native human serum in vitro, ultrafiltrable iron was determined to be 5 to 7.5% of the total serum iron, at different levels of iron:albumin molar ratios. Under similar conditions approximately 2% of iron was ultrafiltrable when predialyzed serum was used. In physiological concentrations, additions of amino acids to predialyzed serum increased ultrafiltrable 59Fe to similar levels as that observed for native human serum. Histidine and lysine showed significant effects in this regard. Amino acids competed effectively with albumin and only to a lesser degree with transferrin for binding of iron, suggesting a tighter affinity of iron for transferrin.
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