Different Areas In Sudan Research Articles

Oral Health Status among Refugees and Internally Displaced Children (3-11 Years Old) from Western Kordofan State, Sudan

Background: Refugees and internally displaced children are often fed food with a low nutritional value or high in carbohydrates, predisposing them to a relatively higher risk of dental caries, and periodontal diseases. Objectives: The aim of this study was to assess the oral health status among a group of refugees and displaced children. Materials and Methods: A descriptive cross-sectional study among (172), 3–13-year-old children (59 boys (34.3%) and 113 girls (65.7%) from in Almoshama camp for displaced and refugees coming from different areas of Sudan and outside Sudan. The participants were selected by non-probability sampling technique based on convenient accessibility and availability of children from friendly child spaces in the camp. Dental caries was assessed by dmft for primary teeth and DMFT for permanent teeth, while periodontal status was assessed by community periodontal index (CPI). Comparison between different non-parametric data was done by Chi-Squire test and parametric data by student T-test, with the level of statistical significance difference set at P-value ≤ 0.05. Results: The prevalence of dental caries among the participants was 33.3%. The mean ±SD dmft score was 2.28±1.77 and the DMFT score was 1.63±1.51. A statistically significant difference in mean DMFT between different age groups (P=0.004) and between boys and girls (P-0.03). While the mean CPI was 0.73. Almost half of the participants had periodontal diseases (54.1%) of whom 37.2% had gingival problems, 14.5% calculus, and only 2.32% have pocket problems. Conclusions: High prevalence of untreated dental caries and periodontal diseases among Refugees and internally displaced children living in Almoshama camp in West Kordofan, Sudan. Emphasis on motivation in oral health promotion campaigns and provision of primary health care services among those vulnerable groups is paramount.

A Study on Geotechnical Properties of Weak Rocks in Sudan

Weak rocks are available in different areas in Sudan. These include sandstone, mudstone, siltstone usually referred to as Nubian Formations (N.F.). Clastic limestones are present in other areas of Sudan. Organic limestones in the form of coralline deposits are present in eastern Sudan, mainly on the Red Sea coast. Weak rocks constitute an important geological feature on which the foundations of most of the important structures are resting. The sandstone is also used as a primary buildingmaterial or as a facing stone over other construction. The geotechnical properties of these formations were investigated bydifferent insitu and laboratory testing methods. This paper summarizes the origin, distribution of Nubian formation and limestone. The paper also presents most of available data on the geotechnical properties of these formations. It is concluded that the Nubian formation and limestone coral deposit are weak rocks with poor quality of core samples; hence feld testingmay give better geocharacterization result for such deposit.

Characterization of Benzene Samples Taken from Different Areas in Sudan Using Laser Induced Breakdown Spectroscopy (LIBS)

Protecting the environment from all types of pollution is a key issue, especially air pollution in particular, because of that, the ecosystem as a whole is under threat. Sudan is one of the vast African countries, covering an area of about one million square miles, and suffering from air pollution. One of the causes of this pollution is the large number of cars and factories that use gasoline. Car exhausts and factory nozzles emit kinds of gases such as carbon monoxide, lead, sulfur and other gases that contribute to air pollution. Due to the large volume of use of gasoline in cars and factories, the low quality of purification and treatment leads to the production of gases that are extremely harmful to the environment. Therefore, it is necessary to examine and determine the components of benzene used in Sudan. The technique of Laser-Induced Breakdown Spectroscopy was used to characterize the benzene samples taken from different regions, and the results showed that they contain carbon and hydrogen that are the main component of benzene, in addition many elements harmful to the environment such as (Pb, Hg and Zn), which can cause cancer diseases, and can also cause great harms to animals, plants and soil.

Screening of Chickpea (Cicer arietinum) Genotypes to Three Identified Races of Fusarium oxysporum f. sp. ciceris in Sudan

Fusarium wilt is the main biotic stress that limited chickpea production in Sudan. Twenty chickpea genotypes were screened for resistance against Fusarium oxysporum f. sp. ciceris (Foc) the causal agent of Fusarium wilt of chickpea. The study was done in pot experiment at Gezira Research Station greenhouse. The genotypes were screened against three identified races of Foc in Sudan namely race 0, 2 and unidentified race to know their reaction against the pathogen.Race 0 is the most prevalent and widely spread in Sudan, while the unidentified race is limited to Gezira State. The variety Hawta (Iccv-92318) showed resistant reaction to the three tested Foc races while Shendi (ILC-1335) and Jabel Marra (ILC-915) varieties showed susceptible reaction to race 0 and highly susceptible reaction to the other two races. The other genotypes showed susceptible reactions to the unidentified race and variable reactions to races 2 and 0. This evaluation has helped to identify new sources of resistance to wilt disease for use in chickpea breeding program and for proper chickpea cultivation in the different areas of Sudan. It also will be useful in preventing the spread of the pathogen from infested areas to uninfected ones.

Assessment of The Effects of Winter Condition on Sweet Sorghum Yield and Sugar Content

Sweet sorghum is an important crop which is produced for food, energy and feed (Almodares and Hadi, 2009). The crop prefers warm moist soil for germination and emergence. However, it would be more beneficial if it can be grown in different seasons. A field experiment was carried to evaluate sixteen sweet sorghum genotypes [Sorghum bicolor (L.) Moench] under winter conditions in order to assess the possibility of producing the crop throughout the year since the crop consumes less water and has a short life cycle when compared with sugarcane. The genotypes we recollected from different areas of Sudan. The experiment was planted using a Randomized Complete Block Design with three replications. There were significant differences among genotypes with respect to the number of days to germination, plant height, number of leaves per plant, chlorophyll content, stem diameter, head weight, shoot fresh weight, head to shoot ratio, brix value, juice weight and number of days to maturity. A highly positive correlation (0.92) was observed between juice and shoot weight, and there was a negative correlation (-0.14) between brix value and head weight. The genotypes showed high variability in all mentioned parameters, hence, could be useful genetic resources for breeding winter adaptation.

Diversity pattern of Duffy binding protein sequence among Duffy-negatives and Duffy-positives in Sudan

BackgroundVivax malaria is a leading public health concern worldwide. Due to the high prevalence of Duffy-negative blood group population, Plasmodium vivax in Africa historically is less attributable and remains a neglected disease. The interaction between Duffy binding protein and its cognate receptor, Duffy antigen receptor for chemokine plays a key role in the invasion of red blood cells and serves as a novel vaccine candidate against P. vivax. However, the polymorphic nature of P. vivax Duffy binding protein (DBP), particularly N-terminal cysteine-rich region (PvDBPII), represents a major obstacle for the successful design of a DBP-based vaccine to enable global protection. In this study, the level of pvdbpII sequence variations, Duffy blood group genotypes, number of haplotypes circulating, and the natural selection at pvdbpII in Sudan isolates were analysed and the implication in terms of DBP-based vaccine design was discussed.MethodsForty-two P. vivax-infected blood samples were collected from patients from different areas of Sudan during 2014–2016. For Duffy blood group genotyping, the fragment that indicates GATA-1 transcription factor binding site of the FY gene (− 33T > C) was amplified by PCR and sequenced by direct sequencing. The region II flanking pvdbpII was PCR amplified and sequenced by direct sequencing. The genetic diversity and natural selection of pvdbpII were done using DnaSP ver 5.0 and MEGA ver 5.0 programs. Based on predominant, non-synonymous, single nucleotide polymorphisms (SNPs), prevalence of Sudanese haplotypes was assessed in global isolates.ResultsTwenty SNPs (14 non-synonymous and 6 synonymous) were identified in pvdbpII among the 42 Sudan P. vivax isolates. Sequence analysis revealed that 11 different PvDBP haplotypes exist in Sudan P. vivax isolates and the region has evolved under positive selection. Among the identified PvDBP haplotypes five PvDBP haplotypes were shared among Duffy-negative as well as Duffy-positive individuals. The high selective pressure was mainly found on the known B cell epitopes (H3) of pvdbpII. Comparison of Sudanese haplotypes, based on 10 predominant non-synonymous SNPs with 10 malaria-endemic countries, demonstrated that Sudanese haplotypes were prevalent in most endemic countries.ConclusionThis is the first pvdbp genetic diversity study from an African country. Sudanese isolates display high haplotype diversity and the gene is under selective pressure. Haplotype analysis indicated that Sudanese haplotypes are a representative sample of the global population. However, studies with a large number of samples are needed. These findings would be valuable for the development of PvDBP-based malaria vaccine.

Peste des petits ruminants infection in domestic ruminants in Sudan

The existence of peste des petits ruminants (PPR) in domestic ruminants and camels in Sudan during 2008-2012 was investigated. Lung tissues and serum samples were randomly collected from sheep, goats, cattle, and camels at different areas of Sudan. A total of 12,384 serum samples were collected from clinically healthy 7413 sheep, 1988 camels, 1501 cattle, 1459 goats, and 23 gazelles at different areas in the Sudan. They were examined for PPR antibodies using competitive ELISA (cELISA). The overall detected seroprevalence of PPR in tested sera was 49.4%; seroprevalence values within species were 67.1, 48.2, 25.8, 2.1, and 21.7% in sheep, goat, cattle, camels, and gazelles, respectively. The highest seroprevalence (68.1%) was observed in sera collected from Darfur states, then the central states (54.3%). A total of 1276 lung tissue samples (623 sheep, 324 cattle, 220 camels, and 109 goats) were collected. The majority of lung samples were collected from clinically healthy animals that showed lesions on PM in slaughterhouses (95%) and during PPR outbreaks; samples were tested for PPR antigen using immunocapture ELISA (IcELISA). PPR antigen was detected in 233 out of the 1276 tested samples (18.3%). Positive results were observed in samples collected from clinically healthy and diseased animals. The observed prevalence values in each species were 33.6, 21.1, 15.4, and 12.3% in camel, goat, sheep, and cattle, respectively. PPR antigen was detected in samples from different areas; however, the highest prevalence (63.9%) was found in samples collected from the eastern states, then Khartoum state (28%). Trials for virus isolation were done in different cell cultures. Out of 30 IcELISA-positive samples inoculated in primary bovine and ovine kidney cells, Vero cells, the PPR virus was successfully isolated from 15 (eight sheep, five camels, and two goats) samples in the three cell culture types. Using RT-PCR, PPRV nucleic acid was detected in all 25 IcELISA-positive tested samples.

Para influenza virus 3 infection in cattle and small ruminants in Sudan

Objective: This study was aimed at elucidating the association between Para influenza virus 3 (PIV3) and respiratory infections in domestic ruminants in different areas of Sudan. Materials and methods: During 2010-2013, five hundred sixty five lung samples with signs of pneumonia were collected from cattle (n=226), sheep (n=316) and goats (n=23) from slaughter houses in different areas in Sudan. The existence of PIV3 antigen was screened in the collected samples using ELISA and Fluorescent antibody technique. PIV3 genome was detected by PCR, and sequence analysis was conducted. Results: Positive results were found in 29 (12.8%) cattle, 31 (9.8%) sheep and 11 (47.8%) goat samples. All the studied areas showed positive results. Highest prevalence (66.7%) was detected in the sheep and goats in Khartoum, followed by in goats in Nyala (33.3%) at western Sudan. Sequence analyses of PIV3 of different regions of Sudan indicated that these were similar in sequence and length. The BLAST analysis indicated that the test sequences were closely related to the available annotated sequences at the GenBank. All these sequences matched with Bovine parainfluenza virus 3 except two those were matching with Swine parainfluenza virus 3. Conclusion: The results prove the existence of PIV3 infection in cattle, sheep and goats in the studied areas in Sudan and suggest its possible role in the respiratory infections. Genetic analysis indicate that the virus is mostly similar with bovine PIV3.

Preliminary Evaluation of Silica Sand in Sudan with Respect to Fracture Sand

Hydraulic fracturing is a well stimulation method performed on reservoirs with low permeability to improve the flow of hydrocarbon into wellbore. Certain chemicals are injected into the well under very high pressure; propping agent, such as sand is added to the fracturing fluid to keep the fracture open. Silica sand was appeared as ideal proppant agent due to its high performance in USA. Many areas in Sudan contain sources of silica sand that may have the potential to act as proppant agent. Recently, no study was conducted to characterize and evaluate the use of this sand as proppant; the current study try to primary evaluate some Sudanese sand with respect to proppant agent. Three samples were collected from different areas in Sudan and a series of laboratory tests were performed according to the API recommended practice API RP 19C. Two samples were taken from Khartoum State (ALMarkhiyat and Hattab zones) and one sample was taken from AL-jaded al- Thawra in Al-jazeera State. The clay content, roundness and sphericity, acid solubility, crush resistance and grain size were measured for all samples. The results of sphericity and roundness show that the sand is satisfy with the API requirements for hydraulic fracturing; the turbidity of samples is also agreed with the API. On the other hand more than 10% of fine has been produced from the different samples under stress of 3000 Psi; which indicate that the sample can be used as proppant for reservoir with the closer pressure less than 3000 Psi; for pressure above 3000 Psi, the samples have to be coated for strength improvement.

Molecular Evidence of High Proportion of Plasmodium vivax Malaria Infection in White Nile Area in Sudan.

Plasmodium falciparum is a predominant malaria species that infects humans in the African continent. A recent WHO report estimated 95% and 5% of P. falciparum and P. vivax malaria cases, respectively, in Sudan. However many laboratory reports from different areas in Sudan indicated otherwise. In order to verify, we selected four hundred suspected malaria cases from Aljabalain area located in the White Nile state, central Sudan, and diagnosed them with quality insured microscopy and species-specific nested PCR. Our results indicated that the proportion of P. vivax infections among suspected malaria cases was high. We found that on average 20% and 36.5% of malaria infections in both study areas were caused by P. vivax using both microscopy and PCR, respectively. This change in pattern is likely due to the recent demographic changes and high rate of immigration from neighbouring countries in the recent years. This is the first extensive clinical study of its kind that shows rising trend in P. vivax malaria cases in White Nile area, Sudan.

Mixed infection of peste des petits ruminants virus (PPRV) and other respiratory viruses in dromedary camels in Sudan, an abattoir study.

This study was intended to determine the role played by peste des petits ruminants (PPR) in causing respiratory infections in camels and its association with other respiratory viruses. A total of 474 lung specimens showing pneumonia were collected from clinically healthy camels in slaughterhouses at five different areas in Sudan. Using immunocapture ELISA (IcELISA), 214 specimens (45.1%) were found to be positive for PPR antigen. The highest prevalence was found in central Sudan (59.9%) then northern Sudan (56.6%) and eastern Sudan (26.6%). Parainfluenza virus 3 (PIV 3), respiratory syncytial virus (RSV), bovine herpes virus-1 (BHV-1), bovine viral diarrhea (BVD), and adenovirus were detected in 4.4, 2.9, 2.0, 9.0, and 1.3% of the specimens, respectively. PPR antigen was found in about 50% of specimens that showed positive result for other viral antigens. Twenty-five of 28 BVD, 15 of 16 PIV3, 8 of 12 RSV, 4 of 4 adenovirus, and 4 of 5 BHV-1 were found in association with other respiratory antigens. Results revealed the existence of PPRV infection in dromedary camels in Sudan and present evidence for mixed virus infection, suggesting that respiratory infections in camels might be exacerbated by PPRV.

Plasmodium falciparum population structure in Sudan post artemisinin-based combination therapy

Over the past decade, Sudan has stepped up malaria control backed by WHO, and this has resulted in significant reduction in parasite rate, malaria morbidity and mortality. The present study analyzed Plasmodium falciparum parasites in four geographical separated areas, to examine whether the success in malaria control following the use of artemisinin-based combination therapy (ACT) has disrupted the population structure and evolution of the parasite.We examined 319 P. falciparum isolates collected between October 2009 and October 2012 in four different areas in Sudan (Jazira [central Sudan], Southern Darfur [western Sudan], Upper Nile [southern Sudan] and Kasala [eastern Sudan]). Twelve microsatellites were analyzed for allelic diversity, multi-locus haplotype and inter-population differentiation. Level of diversity was compared to that detected for three of the above microsatellites among P. falciparum parasites in central and eastern Sudan in 1999, prior to introduction of ACT.Diversity at each locus (unbiased heterozygosity [H]) was high in all areas (Jazira, H=0.67), (Southern Darfur, H=0.71), (Upper Nile, H=0.71), and (Kasala, H=0.63). Microsatellites were distributed widely and private alleles, detected in a single population, were rare. The extent of diversity in the above sites was similar to that seen, in 1999, in central (Khartoum, H=0.73) and eastern Sudan (Gedaref, H=0.75). Significant Linkage disequilibrium (LD) was observed between the microsatellites in all populations. Pairwise FST analysis revealed that parasites in the four areas could be considered as one population. However, the parasites in Sudan clustered away from parasites in West Africa and the Arabian Peninsula.Despite marked reduction in malaria risk in Sudan, the extent of diversity and parasite genetic structure are indicative of a large population size. Further considerable reduction in transmission would be needed before fragmented sub-population can be seen. In addition, the large divergence of P. falciparum in Sudan from West Africa and Arabian Peninsula populations may result from differential evolutionary pressures acting at the population level, which shall be considered in eradication plans.

First report of Bovine Viral Diarrhea Virus antigen from pneumonic cattle in Sudan

To explore the expected role of Bovine Viral Diarrhea Virus (BVDV) in pneumonia in cattle, cattle lungs (n=242) showing signs of pneumonia were collected from slaughter houses of three different localities located at Northern, Central and Western Sudan during 2010–2013. The collected samples were tested for the presence of BVDV antigen using Enzyme-Linked Immunosorbent Assay (ELISA), and Fluorescent Antibody Test (FAT). Twenty six (10.7%) out of 242 samples were found to be positive for BVDV. Positive results were seen in all the three studied areas, with the highest prevalence (16.7%; n=4/24) at Gezira State in Central Sudan. BVDV genome could be detected in all ELISA positive samples. The results indicated the existence of BVDV infection in cattle in different areas in Sudan, and its possible association with respiratory infections in cattle. Analysis using BLAST indicated that the sequence was identical to the previously reported BVDV-1 (GenBank accession AF220247.1.); nucleotide A was found in our study at position 9 of our sequence, whereas T was present instead in the reference virus. This is the first report of detecting BVDV antigen, genome, and its sequence analysis collected from cattle lungs in Sudan.

Determination and assessment of essential elements in Acacia Nilotica-a medicinal and folk uses by different analytical methods

Acacia Nilotica is a good source of several nutrient elements essential for some metabolic processes. The pods of Acacia Nilotica collected from different areas in Sudan have been analysed for 6 minor (Li, Na, K, Mg, Ca, P) and 3 trace (Fe, Zn, Mn) elements by flame photometry and atomic absorption spectrophotometry using the standard addition and calibration curve methods, respectively. The phosphorous, however, was determined U.V. spectrophotometrically using the standard addition method for measurement of the absorbance, at 410 nm of the complex produced by the coupling of meta-molybdate ammonium vanadate with phosphorous. The ash content, density, colour and pH were also determined by standard methods. The results obtained showed that acacia nilotica samples have been found to be especially rich in several nutrient elements such as, K, Fe and P. Concentration of elements are discussed vis-à-vis their medicinal/therapeutic uses. Several statistics like PCA and correlation matrix between elements and pH were performed to the dataset in order to distinguish between geogenic enrichment, lithiogenic, and anthropogenic pollution elements found in the pods. The results suggested anthropogenic and geogenic origin of Na, Ca, P, Fe, Zn, Mn, while Li, K, Mg showed lithogenic and/or geogenic origin and was associated and controlled by mixed origin.

The ethnic distribution of sickle cell disease in Sudan.

Sickle cell disease (SCD) is one of the most common inherited disorders of haemoglobin in Africa and it is expected that sickle cell trait varies in frequency in different areas in Sudan. An extensive literature search was carried out accessing the US National Library of Medicine, the WHO Eastern Mediterranean Region resources, the Catalogue for Transmission Genetics in Arabs and papers and documents published in Sudan that included data on the prevalence of sickle cell anaemia and trait. Rates of SCA and trait varied in different areas in Sudan with the highest rates reported from Western and Eastern Sudan where one in every 123 children born in Messeryia tribe in Western Sudan is at risk of having SCD. High consanguinity rates and malaria endemicity are strong related factors with sickle cell gene in Sudan. This review will present what is known about the rates of sickle cell gene in different ethnic groups in Sudan.

Extensive Quantitative Analysis of Gallstones

Background: The chemical composition of gallstones is essential to study aetiopathogenesis of gallstone disease. Objective: To determine the composition of gallstones in a Sudanese population. Patients and methods: It describes an extensive quantitative analysis of gallstones from patients presented with symptomatic gall stone disease and treated by cholecystectomy after the acceptance of the pre-given informed consent in Khartoum teaching hospital in the period between Jan 2010 and Dec 2010. Using a pretested questionnaire data collected from and analyzed statistically by SPSS computer program version 21. Results: Data are analyzed from 94 patients (six males and 88 females). Cholesterol stones showed a significantly higher cholesterol content than pigment stones (p = 0.0042), though not significantly higher than mixed stones. Their phospholipids content and inorganic phosphates were higher than in the other types of stones and oxalate content was significantly elevated in comparison with mixed stones (p = 0.0402). In mixed stones, the cholesterol, bile acids, and bilirubin were intermediate between cholesterol and pigment stones, whereas triglycerides were significantly more than pigment stones (p = 0.0007). Bilirubin (p = 0.0021) and bile acids (p = 0.0016) were significantly higher than cholesterol stones (p = 0.0001) and (p = 0.0001) respectively. However, they contained the lowest amounts of sodium, potassium, magnesium, and oxalate. In pigment stones, bilirubin was significantly higher (p = 0.0001) than both groups. Conclusion: Collaborations between surgeons, nutritionists, biochemists, and physicians should be stimulated in future studies to define the different types of gall stones in different areas in Sudan and the relevancy of such types with diets’ traditions.

Respiratory syncytial virus infection of camels ( Camelus dromedaries)

This study aimed to investigate the occurrence of respiratory syncytial virus (RSV) infections in camels in Sudan. A total of 272 camel lung specimens showing pneumonia were collected from slaughter houses at four different areas in Sudan, additionally 8 specimens were collected from outbreaks of respiratory infection in camels. Using sandwich ELISA kits for RSV antigen detection 4 out of 280 tested lungs (1.4%) were positive, all were from Central Sudan (Tambool slaughter house). FAT was used to confirm the ELISA positives. Polymerase chain reaction RT/PCR was applied for the detection of RSV genome in camel lungs; 1 out of 4 ELISA positives was positive by RT/PCR. Using indirect ELISA kits 135 out of 495 (27.3%) camel sera showed antibodies to RSV, highest prevalence was observed in Western (33.5%) then Central (31.6%) and Eastern Sudan (23.5%). Based on the manufacturer specified calculations for OD readings, most of positive sera (90/135) were low reactive (1+). This is the first report for the detection of RSV antigen, genome and antibody in camels in Sudan.

Respiratory infection of camels associated with parainfluenza virus 3 in Sudan

This study was undertaken to investigate the role of parainfluenza virus 3 (PIV3) in respiratory infection of camels. A total of 273 lung specimens from camels with pneumonia lesions were collected from slaughterhouses in four different areas of Sudan. In addition, eight specimens were collected from outbreaks of respiratory infection in camels. Using antigen detection sandwich ELISA kits, six out of the 281 specimens tested were positive for the PIV3 antigen (2.1%); the highest prevalence was noted in Eastern Sudan (4.2%), then in Central and Northern Sudan (1.4%). The direct immunofluorescent test (FAT) was used to confirm the positive reactions for PIV3 by ELISA. The polymerase chain reaction (RT-PCR) was applied for the detection of the PIV3 genome in lungs of camels; two out of four samples which were positive by the PIV3 ELISA were also positive by RT-PCR. Virus isolation was attempted for PIV3 in MDBK cells; four specimens yielded cytopathic virus when inoculated onto the cell culture. The cytopathic effect (CPE) consisted of cell rounding, multinucleated cells, sloughing and elongation of cells, and some syncytia were observed on the 3rd to 7th day post-inoculation. Using commercially available indirect ELISA kits for antibodies to PIV3, 495 camel sera were tested, and the seroprevalence detected was 82.2%. The highest seroprevalence was observed in Central (92.6%), then in Eastern (92.2%) and Central to South Sudan (82.5%); the lowest prevalence was found in Northern Sudan (64.8%).

Current situation of Peste des petits ruminants (PPR) in the Sudan

The current situation of PPR in Sudan was investigated. A total of 61 tissue samples were collected from various PPR suspected outbreaks in sheep in Sudan during 2008. Collected tissue samples were tested for PPR antigen using IcELISA, PPR antigen was detected in 26 out of 61 samples (42.6%). Highest antigen detection rate was in specimens collected from western Sudan. A total of 1198 serum samples were collected from sheep (n = 500), camels (n = 392), and goats (n = 306) from different areas in Sudan (Khartoum, Gezira, Tambool, River Nile, Kordofan, White Nile, Blue Nile, Gedarif, Kassala, Halfa ElGadida, Port Sudan). Collected sera were examined for PPR antibodies using cELISA, a total of 336 (67.2%) sheep, 170 (55.6%) goat and 1 (0.3%) camel samples were found to be positive.

Natural exposure of Dromedary camels in Sudan to infectious bovine rhinotracheitis virus (bovine herpes virus-1)

The occurrence of bovine herpes virus-1 (BHV-1) in camels was studied. A total of 186 pneumonic camel lungs were collected from slaughter houses at four different areas in Sudan during 2000–2006. Using sandwich ELISA 1.6% of 186 tested lungs were found positive for BHV-1 antigen, all were from Tambool at Central Sudan. Direct fluorescent antibody test (FAT) was used to confirm the BHV-1 ELISA positives, all ELISA positives were also positive. PCR was used to detect BHV-1 genome with three positive results. BHV-1 was isolated from two camel lungs in MDBK cells. Isolates were identified using ELISA and FAT. Indirect ELISA was used to detect antibodies to BHV-1 in 260 camel sera; 76.9% were found positive. Highest prevalence was observed in sera from Kordofan (84%) then Blue Nile (80%) and Tambool (76.3%). This is the first report for the detection of BHV-1 antigen, genome using PCR, isolation in cell culture and antibodies in camels in Sudan.