Soybean Oil Diet Research Articles

Molecular Characterization, Tissue Distribution Profile, and Nutritional Regulation of acsl Gene Family in Golden Pompano (Trachinotus ovatus).

Long chain acyl-coA synthase (acsl) family genes activate the conversion of long chain fatty acids into acyl-coA to regulate fatty acid metabolism. However, the evolutionary characteristics, tissue expression and nutritional regulation of the acsl gene family are poorly understood in fish. The present study investigated the molecular characterization, tissue expression and nutritional regulation of the acsl gene family in golden pompano (Trachinotus ovatus). The results showed that the coding regions of acsl1, acsl3, acsl4, acsl5 and acsl6 cDNA were 2091 bp, 2142 bp, 2136 bp, 1977 bp and 2007 bp, encoding 697, 714, 712, 659 and 669 amino acids, respectively. Five acsl isoforms divided into two branches, namely, acsl1, acsl5 and acsl6, as well as acsl3 and acsl4. The tissue expression distribution of acsl genes showed that acsl1 and acsl3 are widely expressed in the detected tissues, while acsl4, acsl5 and acsl6 are mainly expressed in the brain. Compared to the fish fed with lard oil diets, the fish fed with soybean oil exhibited high muscular C18 PUFA contents and acsl1 and acsl3 mRNA levels, as well as low muscular SFA contents and acsl4 mRNA levels. High muscular n-3 LC-PUFA contents, and acsl3, acsl4 and acsl6 mRNA levels were observed in the fish fed with fish oil diets compared with those of fish fed with lard oil or soybean oil diets. High n-3 LC-PUFA levels and DHA contents, as well as the acsl3, acsl4 and acsl6 mRNA levels were exhibited in the muscle of fish fed diets with high dietary n-3 LC-PUFA levels. Additionally, the muscular acsl3, acsl4 and acsl6 mRNA expression levels, n-3 LC-PUFA and DHA levels were significantly up-regulated by the increase of dietary DHA proportions. Collectively, the positive relationship among dietary fatty acids, muscular fatty acids and acsl mRNA, indicated that T. ovatus Acsl1 and Acsl3 are beneficial for the C18 PUFA enrichment, and Acsl3, Acsl4 and Acsl6 are for n-3 LC-PUFA and DHA enrichment. The acquisition of fish Acsl potential function in the present study will play the foundation for ameliorating the fatty acids nutrition in farmed fish products.

The Effects of Dietary Soybean Oil on Liver Fat and Visceral Adipose Tissue in Adults With Nonalcoholic Fatty Liver Disease: Study Protocol for a Randomized Control Trial

Abstract Objectives About 25% of adults in the United States have nonalcoholic fatty liver disease (NAFLD) which often accompanies abdominal obesity. Blood biomarkers of linoleic acid (LA), an essential omega-6 fatty acid, are inversely associated with visceral adipose tissue, liver fat, and the risk of NAFLD. To our knowledge, the effect of increasing dietary LA on ectopic fat in the liver and visceral adipose tissue in adults with NAFLD while maintaining weight has not been investigated. The objective of the Weight, Energy, Lipids and the Liver (WELL) study is to determine the impact of dietary LA-rich soybean oil on ectopic liver fat and visceral adipose tissue in adults with NAFLD. Methods In this 16-week parallel arm randomized control trial, 58 adults with NAFLD will be randomly assigned to consume 3 study foods per day made with either 10g of soybean oil each or 10g of palm oil each (30g of oil per day). Prior to randomization, participants will complete a 2-week run-in period to become acclimated by consuming 3 study foods per day, each delivering 10 g of an oil mixture (safflower oil and shortening in a 3.4:1 ratio). Participants will receive counseling from a registered dietitian to incorporate the study foods into their diets while maintaining their body weight. Self-reported study food consumption will be collected daily. Body weight will be measured every two weeks. Dietary intake and physical activity will be assessed through 24 hour recalls. Fasting blood will be collected every two weeks to measure fatty acids, cholesterol, triglycerides and markers of inflammation and glycemia. Magnetic Resonance Imaging (MRI) will be used to assess liver and visceral fat before and after the 16-week randomized intervention period. Additionally, postprandial cholesterol, triglycerides and markers of inflammation and glycemia will be measured before and after the randomized intervention period. Results We hypothesize that consumption of the study foods made with LA-rich soybean oil (30g/day) will result in a reduction in hepatic ectopic fat and visceral adipose tissue, without altering body weight. Conclusions Reducing liver fat and visceral adipose tissue through consumption of a LA-rich soybean oil may ultimately lead to improved cardiometabolic outcomes for adults with NAFLD. Funding Sources The United Soybean Board.

Beneficial effects of re-feeding high α-linolenic acid diets on the muscle quality, cold temperature and disease resistance of tilapia

To investigate the effects of re-feeding high α-linolenic acid (ALA) diets on the muscle quality, cold temperature and disease resistance of the tilapia with nutritional history of soybean oil diets, three experimental diets with linoleic aicd (LA)/ALA ratios at 9 (D1, taking soybean oil as lipid sources), 3 and 1 (D2 and D3, taking soybean and linseed oils as lipid sources) were prepared to re-feed juveniles for 10 weeks, and the growth performance, muscle quality were analyzed. After the re-feeding trial, the fish were fasted for 8 weeks at cold temperature (15°C–20 °C) and then subjected to the Aeromonas hydrophila challenge, and the cold temperature and disease resistance of the fish were evaluated. It was shown that a comparable growth performance was detected among the three dietary groups, while, the high feed efficiency and low viscerosomatic and hepatosomatic index were detected in the D2 and D3 groups compared with the D1 group. In addition, the docosahexaenoic acid (DHA) and n-3 polyunsaturated fatty acid (PUFA) levels of the muscle increased in a parallel pattern with the dietary ALA levels, and the muscular tenderness, adhesiveness, and chewiness were modified substantially in fish fed the diets D2 and D3. After 8-week fasting at cold temperature, the low serum total antioxidant capacity (T-AOC) and malondialdehyde (MDA) levels, and high serum lysozyme (LZM) and C3 levels were observed in the D2 and D3 groups compared with the D1 group. It was also shown that high eicosapentaenoic acid (EPA), DHA, and n-3 PUFA levels were observed in the intestine, liver, and spleen of fish from D2 and D3 groups. Correspondingly, in the fish of the D2 and D3 groups, the mRNA levels of lzm in the liver, intestine, and spleen, and c3 in the intestine and spleen were increased, while the mRNA levels of il-1β, ifn-γ, and tnf-α in the intestine, and ifn-γ, tnf-α in the liver, as well as spleen il-1β, were decreased. Furthermore, the survival at day 15 post-challenge of A. hydrophila in the D2 and D3 groups were higher than those of the D1 group. The results demonstrated that re-feeding high ALA diets were beneficial to the muscle quality, cold temperature and disease resistance in the tilapia, and provide a basis for selecting the dietary lipid sources of tilapia pre-winter feed.

Effects of selected dietary plant oils on survival, growth, fatty acid profile, and enzyme activity of a tropical hybrid carp (Barbonymus gonionotus ♀ × Hypsibarbus wetmorei ♂) larvae

Hybrid lemon fin barb is a new tropical carp species developed by crossing silver barb (Barbonymus gonionotus, ♀) with lemon fin barb (Hypsibarbus wetmorei, ♂) to cover the production gap in Malaysian aquaculture, as the tasty wild H. wetmorei is being overexploited. Different plant oils such as crude palm oil (CPO), linseed oil (LO), canola oil, sunflower seed oil and soybean oil were tested for the larval stage of this fast-growing and delicious carp species. Three-day old hybrid larvae were arbitrarily assigned to twenty 5-L tanks with a rate of 50 larvae L−1. After 20 days of the feeding trial, weight gain, total length gain and specific growth rate (SGR) of hybrid larvae fed diets containing CPO and LO were significantly higher (p < 0.05) than those of larvae fed with soybean oil. Survival of larvae fed CPO was significantly higher (p < 0.05) than those of larvae fed LO, canola oil and soybean oil diets. Total saturated fatty acids (SFAs) reported were high in hybrid larvae fed soybean oil followed by CPO while larvae fed CPO had the highest (p < 0.05) total body monounsaturated fatty acids (MUFAs). Total polyunsaturated fatty acids (PUFAs) of larvae fed CPO, LO, sunflower seed oil and soybean oil were significantly reduced (p < 0.05) accordingly. The larvae fed the CPO diet had the highest (p < 0.05) docosahexaenoic acid (DHA), arachidonic acid (ARA), total n-3 fatty acids and n-3/ n-6 ratio while dietary CPO and linseed oil increased larval body eicosapentaenoic acid (EPA) content. There was a significant interaction (p < 0.05) in dietary linoleic acid (LA) and ARA provided by different plant oils on ARA in hybrid larval body. Moreover, among plant oils used in the experiment, CPO was fetched at the lowest price. In conclusion, the use of dietary CPO in hybrid lemon fin barb larval diets is more economical and feasible.

Evaluation of nutritional quality and fatty acid profile of carp (Cyprinus carpio) fed with diet based on oil sources

Background: The Cyprinus carpio as commercial fish in Iran contains nutritive components and plays an important role in human health. The present study research evaluated the nutritive quality of carp including proximate and fatty acid compositions. Objective: The aim of the present study was to investigate the effects of diet based on oil sources on the fatty acids composition of fish fillets of Cyprinus carpio. Methods: The carp (Cyprinus carpio) were fed with four diets based on soybean oil (SO), palm oil (PO), fish oil (FO), and (soybean and palm) oil (SPO). Results: The quality of fatty acids in the body of fish fed with SO and PO diets was found significantly better (p &lt;0.05) than those fish fed with FO diet. Total SFA (Saturated fatty acids) was highest significant in the fillet fat of the carp fish fed with PO diet, but there were no significant differences between the MUFA (Mono unsaturated fatty acids), PUFA (Poly unsaturated fatty acids), HUFA (High unsaturated fatty acids), and n-3/n-6 fatty acids of fillet in the carp fish fed with the experimental diets. Fillet fatty acid composition reflected dietary fatty acid profile. The highest level of α-linolenic acid, linoleic acid, and oleic acid was observed in fish fillets fed FO and SO, respectively. Although the eicosapentaenoic acid level of fish fillets fed diets FO and SPO was higher than other treatments, no significant difference was found in docosahexaenoic acid content among all dietary treatments. Conclusion: The present study showed that the suitable diet fat sources (SO, PO, SPO) fed by the carp, Cyprinus carpio, for 56 days, was suitable their productivity.

Evaluation of essential fatty acids in lactating sow diets on sow reproductive performance, colostrum and milk composition, and piglet survivability.

Mixed parity sows (n = 3,451; PIC, Hendersonville, TN; parities 2 through 9) and their litters were used to evaluate the effects of essential fatty acid (EFA) intake on sow reproductive performance, piglet growth and survivability, and colostrum and milk composition. Our hypothesis, like observed in earlier research, was that increasing linoleic acid (LA) and α-linolenic acid (ALA) would improve sow and litter performance. At approximately day 112 of gestation, sows were randomly assigned within parity groups to 1 of 4 corn-soybean meal-wheat-based lactation diets that contained 0.5 (Control) or 3% choice white grease (CWG), 3% soybean oil (SO), or a combination of 3% soybean oil and 2% choice white grease (Combination). Thus, sows were provided diets with low LA and ALA in diets with CWG or high LA and ALA in diets that included soybean oil. Sows received their assigned EFA treatments until weaning and were then fed a common gestation and lactation diet in the subsequent reproductive cycle. Average daily feed intake during the lactation period increased (P < 0.05) for sows fed the Combination and CWG diets compared with sows fed the Control or SO diet. However, daily LA and ALA intakes of sows fed the Combination and SO diets were still greater (P < 0.05) than those of sows fed 0.5 or 3% CWG. Overall, sows consuming high EFA from the Combination or SO diets produced litters with heavier (P < 0.05) piglet weaning weights and greater (P < 0.05) litter ADG when compared with litters from sows fed diets with CWG that provided low EFA. Despite advantages in growth performance, there was no impact of sow EFA intake on piglet survivability (P > 0.10). Additionally, lactation diet EFA composition did not influence sow colostrum or milk dry matter, crude protein, or crude fat content (P > 0.10). However, LA and ALA content in colostrum and milk increased (P < 0.05) in response to elevated dietary EFA from SO. There was no evidence for differences (P > 0.10) in subsequent sow reproductive or litter performance due to previous lactation EFA intake. In conclusion, increased LA and ALA intake provided by soybean oil during lactation increased overall litter growth and pig weaning weights, reduced sow ADFI, but did not affect piglet survivability or subsequent performance of sows.

Regulation of Δ6Fads2 Gene Involved in LC-PUFA Biosynthesis Subjected to Fatty Acid in Large Yellow Croaker (Larimichthys crocea) and Rainbow Trout (Oncorhynchus mykiss).

Δ6 fatty acyl desaturase (Δ6Fads2) is regarded as the first rate-limiting desaturase that catalyzes the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA) from 18-carbon fatty acid in vertebrates, but the underlying regulatory mechanism of fads2 has not been comprehensively understood. This study aimed to investigate the regulation role of fads2 subjected to fatty acid in large yellow croaker and rainbow trout. In vivo, large yellow croaker and rainbow trout were fed a fish oil (FO) diet, a soybean oil (SO) diet or a linseed oil (LO) diet for 10 weeks. The results show that LO and SO can significantly increase fads2 expression (p < 0.05). In vitro experiments were conducted in HEK293T cells or primary hepatocytes to determine the transcriptional regulation of fads2. The results show that CCAAT/enhancer-binding protein α (C/EBPα) can up-regulate fads2 expression. GATA binding protein 3 (GATA3) can up-regulate fads2 expression in rainbow trout but showed opposite effect in large yellow croaker. Furthermore, C/EBPα protein levels were significantly increased by LO and SO (p < 0.05), gata3 expression was increased in rainbow trout by LO but decreased in large yellow croaker by LO and SO. In conclusion, we revealed that FO replaced by LO and SO increased fads2 expression through a C/EBPα and GATA3 dependent mechanism in large yellow croaker and rainbow trout. This study might provide critical insights into the regulatory mechanisms of fads2 expression and LC-PUFA biosynthesis.

65 Evaluation of Essential Fatty Acids in Lactating Sow Diets on Sow Reproductive Performance, Colostrum and Milk Composition, and Pre-Weaning Litter Growth and Survivability

Abstract Mixed parity sows (n = 3,451; PIC, Hendersonville, TN) and their litters were used to evaluate the effects of essential fatty acid (EFA) intake on sow reproductive performance, piglet growth and survivability, and colostrum and milk composition. At approximately d 112 of gestation, sows were randomly assigned to 1 of 4 corn-soybean meal-wheat-based lactation diets with 0.5 (Control) or 3% choice white grease (CWG), 3% soybean oil (SO), or a combination of 3% soybean oil and 2% choice white grease (Combination). Thus, sows were provided diets with low EFA [linoleic acid (LA) and α-linolenic acid (ALA)] in diets with CWG or high EFA in diets with SO. Lactation ADFI increased (P &amp;lt; 0.05) for sows fed the Combination and CWG diets compared with sows fed the Control or SO diet. Daily LA and ALA intake of sows fed the Combination and SO diets were greater (P &amp;lt; 0.05) than sows fed 0.5 or 3% CWG. Overall, sows consuming high EFA from the Combination or SO diets produced litters with heavier (P &amp;lt; 0.05) piglet weaning weights and greater (P &amp;lt; 0.05) litter ADG when compared with litters from sows fed diets with low EFA provided through CWG. There was no effect of sow EFA intake on piglet survivability (P &amp;gt; 0.10). Lactation diet EFA concentration did not influence colostrum or milk dry matter, crude protein, or crude fat content (P&amp;gt;0.10). However, LA and ALA content in colostrum and milk at weaning increased (P &amp;lt; 0.05) in response to elevated dietary EFA from SO. There was no evidence for differences (P &amp;gt; 0.10) in subsequent sow reproductive or litter performance due to previous lactation EFA intake. In conclusion, increased LA and ALA intake provided by SO during lactation increased overall litter growth and pig weaning weights but did not affect piglet survivability or subsequent performance.

246 Awardee Talk: Evaluating the Impact of Essential Fatty Acids in Lactation Diets on Sow and Litter Performance

Abstract Two experiments were conducted to evaluate the effects of supplemental fat sources providing low and high essential fatty acids (EFA) in lactation diets on sow reproductive performance, piglet growth and survivability, and colostrum and milk composition. In Exp. 1, mixed parity sows (n = 91) were allotted to 5 experimental treatments arranged in a 2×2 + 1 factorial. Treatments were corn-soybean meal-based diets that contained no added fat or diets with 3% added fat as either beef tallow or soybean oil starting on d 107 or 112 of gestation. Sows were provided low EFA [linoleic acid (LA) and α-linolenic acid (ALA)] in diets without supplemental fat or with beef tallow or high EFA with soybean oil. Results confirmed that providing fat sources with high EFA can increase colostrum and milk LA and ALA content, regardless of pre-farrow time of diet consumption. However, supplemental fat did not influence litter growth performance (P &amp;gt; 0.05). In Exp. 2, mixed parity sows (n = 3,451) were assigned to 4 corn-soybean meal-wheat-based lactation diets with 0.5 (Control) or 3% choice white grease (CWG), 3% soybean oil (SO), or a combination of 3% soybean oil and 2% choice white grease (Combination) at d 112 of gestation to provide diets with low or high EFA. Lactation ADFI increased (P &amp;lt; 0.05) for sows fed the Combination and CWG diets compared with sows fed the Control or SO diet. Sows fed Combination and SO diets had greater (P &amp;lt; 0.05) LA and ALA intake, LA and ALA in colostrum and milk, litter weaning weights, and litter ADG than sows fed 0.5 or 3% CWG. Sow EFA intake did not affect (P &amp;gt; 0.10) piglet survivability or subsequent sow reproductive performance. In conclusion, increased LA and ALA intake provided by SO during lactation increased overall litter growth and weaning weights but did not affect piglet survivability or subsequent performance.

Effects of Dietary Lipid Sources on Alleviating the Negative Impacts Induced by the Fishmeal Replacement With Clostridium autoethanogenum Protein in the Diet of Pacific White Shrimp (Litopenaeus vannamei)

This study evaluated the effects of lipid sources on the replacement of fishmeal with Clostridium autoethanogenum protein (CAP) in the diet of Pacific white shrimp (Litopenaeus vannamei). By using CAP to replace 40% fishmeal in the basal diet (contains 25% fishmeal), four diets (PC as the control diet, LSO as the low soybean oil level, MSO as the middle soybean oil level, and HSO as the high soybean oil level) were formulated by adding different proportions of fish oil and soybean oil. Each diet was assigned to four replicates (40 shrimps per replicate, initial weight = 1.79 ± 0.02 g). A 7-day Vibrio parahaemolyticus challenge test was conducted at the end of the 50-day feeding experiment. Weight gain was significantly higher and the cumulative mortality after challenge was lower in HSO treatment. The total antioxidant capacity (T-AOC) in hepatopancreas was significantly raised with increasing dietary soybean oil based on CAP substitution. After challenge, Pen3 mRNA expression was significantly higher in hepatopancreas and NF-κB pathway-related mRNA (dorsal, relish, and IKKβ) expression was higher in the intestine in HSO treatment. The height of mucosal folds and the thickness of the muscle layer were positively correlated with the level of dietary soybean oil. Transmission electron microscopy (TEM) analysis showed that the microvilli were damaged and the endoplasmic reticulum was swollen in shrimp fed the LSO diet. Diversity of intestinal microbiota was increased in shrimp fed the LSO diet, with a significant increase in beneficial bacteria such as Halocynthiibacter and Ruegeria as well as less harmful bacteria such as Vibrio. These results suggested that the replacement of fishmeal with CAP and the high proportion of soybean oil in the diet could improve the growth performance, disease resistance, and intestinal structure of Pacific white shrimp, and high fish oil in a low-FM diet raised the percentage of beneficial bacteria in the intestine of shrimp.

Effects of in ovo feeding and dietary addition oils on growth performance and immune function of broiler chickens

This study aimed to investigate the effects of in ovo feeding (IOF) and dietary addition (DA) oils on growth, development and immune function of broiler chickens. In experiment 1, a total of 500 eggs were randomly assigned to 3 treatments: non-injected group (CON) with 100 eggs; soybean oil injected group (SO) with 200 eggs and linseed oil injected group (LO) with 200 eggs. Results showed that there were no detrimental effects of IOF of oils on embryonic development. In experiment 2, a two factor experimental design was adopted. After hatching, 120 chicks which came from each oil-injected group were divided into 2 treatments with 6 replicates, and chickens were fed soybean oil diet and linseed oil diet, respectively. The results showed that DA linseed oil increased final body weight (FBW) of broilers at d 21 post hatch, IOF of linseed oil decreased average daily feed intake (ADFI) and feed conversion ratio (FCR) of broilers from d 1 to 21 (P < 0.05), while the plasma leptin level of 21-day-old broilers was increased by IOF or DA linseed oil (P < 0.05). Main effect analysis showed that DA linseed oil increased the spleen index and mRNA expression of IFN-γ in spleen of broilers at 7 d of age (P < 0.05). IOF of linseed oil upregulated the mRNA expression of IFN-γ in the spleen of chicks at 1 d and mRNA expression of IL-2 and IL-4 in spleen of broilers at 21 d (P < 0.05), and the interaction effect showed that IOF and DA linseed oil synergically increased the expression of IL-2 and IL-4 in spleen of broilers at 21 d. Compared with SO group, LO increased the Shannon index of hatching-day cecum microflora (P < 0.05). Principal co-ordinates analysis (PcoA) showed that LO group clearly separated from CON and SO groups. Finally, Spearman correlation analysis also manifested that Alkalicoccus was significantly correlated with spleen index and mRNA expression of IL-2, and Phreatobacter was significantly correlated with the mRNA expression of IL-2 and IFN-γ in spleen, Acinetobacter had a positive correlation with thymus index (P < 0.05). In conclusion, IOF of linseed oil reduced the ADFI and FCR of broilers and increased the species diversity and changed the structure of cecal microflora of chicken embryos at the 19th day of incubation (E19). Immune function of broilers spleen was also regulated by IOF and DA linseed oil.

Grape seed tannin extract and polyunsaturated fatty acids affect in vitro ruminal fermentation and methane production.

Condensed tannins (CT), one of the most ubiquitous compounds in the plant kingdom, can modulate ruminal nutrient metabolism. Objectives were to study potential interactions of CT and polyunsaturated fatty acids (PUFA) on ruminal fermentation, biohydrogenation (BH), and methane production. Ruminal fluid obtained from lactating Holstein Friesian cows was used. All experiments were carried out as a completely randomized design with the same mixed diet: control (60:40 forage:concentrate) without supplement (CON), 2.5% soybean oil (SBO), and SBO + grape seed tannin extract (GSTE) at 0.2%, 0.4%, 0.6%, or 0.8% dietary DM (ST0.2, ST0.4, ST0.6, and ST0.8, respectively). Compared with CON (84.7mM), total VFA concentration was not affected by SBO, but decreased (P < 0.001) with ST0.8 vs. ST0.6 (75.3 vs. 78.3mM). Relative to CON, methane production was depressed (P < 0.001) by 17.7% and 28.0% in ST0.4 and ST0.8. The highest (P < 0.001) mean concentrations of c9,t11 CLA and C18:1 t11 were observed with ST0.4 compared with CON, but there was no difference between SBO and CT-containing diets. Disappearance of C18:2 c9,c12 was 49.1% vs. 50.3% in CON vs. SBO, whereas it ranged from 39.9% to 46.3% in CT-containing diets after 2h incubation (P < 0.001). Concentrations of c9,t11 CLA with supplemental SBO and ST0.8 nearly peaked (P < 0.001) at 2h incubation, but this fatty acid peaked (P > 0.05) at 6h incubation and remained higher (P < 0.001; 15.9-17.0 µg/mL) at 24h incubation with ST0.2, ST0.4, and ST0.6 compared with other diets (13.5-14.5 µg/mL). Compared with CON (50.6 µg/mL), concentration of C18:1 t11 with SBO and CT-containing diets reached a peak (P < 0.001; 241-265 µg/mL) at 12h incubation. Concentration of C18:0 was 171%-231% higher (P < 0.001) with SBO and CT relative to CON at 24h incubation. Overall, these results demonstrated that PUFA in SBO are more effective in modulating ruminal BH and CH4 production when combined with CT. However, high doses of added CT can reduce ruminal VFA concentration. Thus, a level of 0.4% GSTE added to diets containing 2.5% PUFA from plant origin might be suitable for optimizing ruminal fermentation and BH of C18:2 c9,c12 to fatty acid intermediates that could have beneficial effects to human health.

Effects of dietary sacha inchi (Plukenetia volubilis L.) oil and medicinal plant powder supplementation on growth performance, carcass traits, and breast meat quality of colored broiler chickens raised in Vietnam.

The present study aimed to investigate the effects of replacing dietary soybean oil (SBO) with sacha inchi (Plukenetia volubilis L.) oil (SIO) supplemented or not with medicinal plant powder (MP, 60% cinnamon twig, and 40% star anise fruits) on broiler performance, carcass traits, and omega-3 polyunsaturated fatty acid (n-3 PUFA) content of breast meat. A total of 288 Ho × Luong Phuong broiler chickens (age and average body weight: 6weeks old and 877 ± 13.4g) were equally divided into three groups (6 replicates of 16 birds each), balanced by BW and sex. Each group was randomly allocated to one of three dietary treatments: a 2% SBO diet (CON), a 2% SIO diet (SI), and a diet supplemented with 2% SIO and 1% MP (SIM). The experiment lasted for 70days. Broiler performance, carcass traits, and technological meat quality were not affected by the diets (P > 0.05). However, colored broiler chickens fed the SIM diet had increased empty gizzard percentage (P < 0.05) compared to those fed the CON diet. Especially, the n-3 PUFA content of breast meat from broiler chickens fed diets containing SIO was higher than those of birds fed CON diet (P < 0.01). A significant decrease in cholesterol content was observed (P < 0.01) in broilers fed SIM diet compared to those fed CON diet. In conclusion, replacing 2% SBO with 2% SIO and 1% MP supplementation in broiler diets increased n-3 PUFA content and decreased cholesterol content in breast meat, without negative effects on bird performance, carcass characteristics, and meat quality. Therefore, a combination of SIO and MP can be used as an effective strategy to ameliorate the meat quality of finishing broiler chickens by enhancing n-3 PUFA content and reducing the cholesterol content of breast meat.

Molecular characterization and expression analysis of acyl-CoA synthetase 6 in golden pompano Trachinotus ovatus reveal its function in DHA enrichment

Acyl-CoA synthetase 6 (Acsl6) involves the enrichment of docosahexaenoic acid (DHA) and lipid synthesis in mammals, while its molecular function is still unknown in teleost. To explore the potential function of teleost Acsl6, this study investigated the molecular characterization and expression analysis of the acsl6 gene in the marine fish golden pompano (Trachinotus ovatus). The acsl6 cDNA cloned has a coding region (2169 bp) encoding 722 amino acids, which possesses a typical structure of Acsl including the gate domain and linker motif. The highest acsl6 mRNA expression was detected in the brain, followed in eyes, muscle, intestine, liver, and other tissues. Comparing to the fish fed with lard oil or soybean oil diets, the fish fed with fish oil exhibited high muscular DHA contents, and acsl6 mRNA levels (p < 0.05). Additionally, feeding T. ovatus three experimental diets containing different n-3 LC-PUFA levels (5.25%, 10.05%, and 14.05%) showed that the muscular DHA and n-3 LC-PUFA contents, as well as the acsl6 mRNA levels, matched the dietary n-3 LC-PUFA levels. The transcript level of acsl6 and contents of DHA in the muscle was significantly affected by dietary DHA/EPA ratios, and high DHA/EPA ratios increased the muscular DHA contents and acsl6 gene expression (p < 0.05). Moreover, the over-expression of acsl6 promoted the DHA contents of hepatocytes and enterocytes, suggesting that T. ovatus Acsl6 plays an active role in the deposition of DHA content. Collectively, the positive relationship among dietary DHA, muscular acsl6 mRNA and DHA levels, as well as the increase of cellular DHA contents by over-expression of acsl6, indicated that Acsl6 is beneficial to the DHA enrichment in T. ovatus. The acquisition of fish Acsl6 potential function in the present study will play the foundation for ameliorating the DHA contents in farmed fish products.

Comparative effects of dietary soybean oil and fish oil on the growth performance, fatty acid composition and lipid metabolic signaling of grass carp, Ctenopharyngodon idella

Lipid was an essential constituent of aquatic feeds, among which fish oil (FO) was the most excellent lipid source for its nutritive values. However, high price and limited production restricted its application, thus leading to the consideration of developing other alternative lipid sources, and soybean oil (SO) instead of FO was applied as the main lipid source in the commercial feed of grass carp. In the present study, two typical lipids sources FO and SO were used to prepare grass carp feed, and their comparative effects on the growth performance, nutrient composition and metabolic signaling were investigated. The growth performance of two groups exhibited no significant difference, while crude lipid was much higher in the muscle of SO group. Additionally, muscle fatty acid composition of both groups were highly correlated with that of diets, and the proportion of muscle n-3 polyunsaturated fatty acids was notably higher in the FO group, especially that of C20:5n-3 (EPA) and C22:6n-3 (DHA). Comparative digital gene expression profiles (DGE) was conducted and 1261 differentially expressed genes (DEGs) had been identified, then gene ontology (GO) enrichment analysis revealed the enriched functional subterms were primarily focused on cellular metabolic processes and regulation. KEGG pathway analysis elucidated the main metabolism related pathways, and DEGs involved in fatty acid elongation, fatty acid metabolism, and glycerolipid metabolism pathways were listed, including elongation of very long chain fatty acids protein 7 (el7), lysophospholipid acyltransferase 4 (lpcat4), long-chain acyl-CoA synthetase (acs), phosphatidate phosphatase LPIN (lpin), cathepsin L, and glucose-6-phosphate isomerase (gpi) et al. In conclusion, the nutritional composition rather than growth performance of grass carp was affected by different lipid sources, and the underlying regulatory signaling was predicted, which would provide theoretical basis for fish diet formulation.

Evaluation of Essential Fatty Acids in Lactating Sow Diets on Sow Reproductive Performance, Colostrum and Milk Composition, and Piglet Survivability

A total of 3,451 mixed parity sows and their litters were used to evaluate the effects of essential fatty acid intake on sow reproductive performance, piglet growth and survivability, and colostrum and milk composition. At approximately d 112 of gestation, sows were blocked by parity within farrowing room and randomly assigned to 1 of 4 experimental treatments. Lactation diets were corn-soybean meal-wheat-based and included 0.5 (Control) or 3% choice white grease (CWG), 3% soybean oil (SO), or a combination of 3% soybean oil and 2% choice white grease (Combination). Thus, sows were provided diets with low essential fatty acid (EFA; as linoleic [LA] and α-linolenic acid [ALA]) in diets with choice white grease or high EFA in diets with soybean oil. Prior to farrowing, sows were provided 4 lb/d of their assigned lactation diet and then allowed ad libitum access after parturition. Overall lactation ADFI increased (P < 0.001) when sows were fed the Combination and CWG treatments compared to sows fed the Control or diet with 3% SO. Regardless of differences among ADFI, daily LA and ALA intake of sows assigned to the Combination and SO treatments were greater (P < 0.001) than sows fed diets with lower EFA provided as CWG. There was no effect of sow EFA intake on piglet survivability from birth to 24 h or from 24 h to weaning (P > 0.10). Overall, sows consuming high EFA provided in the Combination and SO diets produced litters with greater (P < 0.05) litter gain and litter ADG during the lactation period and heavier (P < 0.001) piglet weaning weights when compared to litters from sows fed diets with low EFA provided through CWG. Lactation diet EFA composition did not influence colostrum or milk dry matter, crude protein, or crude fat content (P > 0.10). However, LA and ALA content in both colostrum and milk at weaning increased (P < 0.05) in response to increased EFA levels in diets that contained SO. There was no evidence for differences (P > 0.10) in wean-to-estrus interval, percentage of sows bred by d 7, percentage of sows bred by d 12, farrowing rate, or subsequent farrowing performance due to sow lactation EFA intake. In conclusion, increased LA and ALA intake during the lactation period from soybean oil addition increased overall litter growth and average weaning weights of pigs but did not affect piglet survivability or subsequent reproductive performance of sows.

Effect of Type and Dietary Fat Content on Rabbit Growing Performance and Nutrient Retention from 34 to 63 Days Old.

Simple SummaryAn increase in the fat content of the diet increases the dietary energy concentration, and consequently, the growth and feed efficiency of the animals. The fatty acids (FA) profile of the fat source can also affect animal body composition. The purpose of this study was to test the effect of some fat sources added at different levels in growing rabbit feed. In this study, it was observed that the increment of dietary fat improved nitrogen efficiency utilization and reduced nitrogen excretion; the fat source also affected animal performance and mortality rate.The study was carried out on individually and collectively housed growing rabbits from 34 to 63 days of age. Two experiments were conducted using three fat sources: Soybean oil (SBO), Soya Lecithin Oil (SLO), and Lard (L; Exp. 1), and SBO, Fish Oil (FO), and Palm kernel Oil (PKO; Exp. 2), added at two inclusion levels (1.5 and 4.0%). In both trials, 180 rabbits were housed in individual cages and additional 600 rabbits in collective cages from day 34 to 63. Animals fed with 4% dietary fat showed lower Daily Feed Intake (DFI) and Feed Conversion Ratio (FCR) than those fed with 1.5%, except in the individually housed animals in Exp. 1. In the collective housed group in Exp. 1, DFI was a 4.8% higher in animals fed with diets containing lard than those fed with SBO (p = 0.036). Lard inclusion also tended to reduce mortality (p = 0.067) by 60% and 25% compared with SBO and SLO diets, respectively. Mortality was the highest with the higher level of soya lecithin (14% vs. 1%, p < 0.01). A similar mortality rate was observed in the lowest level of SBO. In the grouped-housed animals in Exp. 2, a decrease of DFI (−12.4%), Bodyweight (BW) at 63 d (−4.8%), and Daily Weight Gain (DWG) (−7.8%) were observed with the inclusion of fish oil (p < 0.01) compared to other fat sources. Fish oil also tended to increase (p = 0.078) mortality (13.2%) compared with palm kernel oil (6.45%); similar results were found when animals were individually housed. The overall efficiency of N retention (NRE) increased with the highest level of fat in Exp. 1 (34.9 vs. 37.8%; p < 0.0001). It can be concluded that lard and palm kernel oil are alternative sources of fat due to the reduction of mortality. The inclusion of fish oil impaired animal productivity and increased mortality. An increment of the dietary fat level improved FCR and overall protein retention efficiency.

The effects of different lipid sources on the growth, intestinal health, and lipid metabolism of the pacific white shrimp (Litopenaeus vannamei)

It is an urgent problem to find suitable replacement of fish oil (FO) in aquatic feeds due to the stagnant production and rising prices of FO. To evaluate the substitution of vegetable oils for FO in the diet of Litopenaeus vannamei (L. vannamei), four diets containing FO, linseed oil (LO), soybean oil (SO) or rapeseed oil (RO) have been fed to L. vannamei for 30 days in this study, and the effects of different lipid sources on the growth, body composition, intestinal health, disease resistance and lipid metabolism of the shrimps were investigated. The results showed that the profiles of fatty acids in four diets and the muscles of the shrimps were discrepant. Further analysis demonstrated that the growth performance of the shrimps in LO and SO groups was similar as that in FO group. Moreover, it seemed that the intestinal health in the shrimps fed with LO or SO diet was improved. In contrast, RO diet reduced the weight gain rate and weakened the intestinal health of the shrimps. Consistently, the shrimps fed with RO diet displayed the highest mortality after Vibrio parahaemolyticus (V. parahaemolyticus) infection among all groups. Additionally, the contents of total cholesterol (T-CHO) and the gene expression of fatty acid synthase (FAS) in the hepatopancreas of the shrimps in RO group was significantly higher than that in FO group. In conclusion, LO or SO could be utilized as the substitution for FO in the diet of L. vannamei, while the replacement of FO with RO in the diet might induce the impaired intestinal health and the imbalance of lipid metabolism, leading to the reduced growth and disease resistance of the shrimp. The present study has provided the research basis for the further development of vegetable oils as a substitute for fish oil in shrimp diets.

Effects of an array of dietary treatments and length of feeding on ruminal methane emission and other variables in hair sheep

Eighteen Katahdin sheep ewes (initial body weight [BW] = 73.7 ± 1.76 kg and age = 5.2 ± 0.56 yr) and 18 St. Croix ewes (54.7 ± 1.33 kg BW and 4.1 ± 0.42 yr) were used in an experiment to determine effects of dietary level of Sericea lespedeza (Lespedeza cuneata; 5.8% condensed tannins and 10.3 % crude protein, dry matter [DM] basis) and other supplemental ingredients on BW, feed intake, digestion, energy metabolism, ruminal methane emission, and levels of blood and ruminal fluid constituents. Diets were consumed ad libitum and included a concentrate supplement at approximately 0.45 % BW (DM). Alfalfa (Medicago sativa) was the basal forage for control (CON), ionophore (ION; lasalocid at 33 mg/kg DM), coconut oil (3%; CCO), and soybean oil (3%; SBO) diets, and forage in moderate- and high-lespedeza diets was a 1:1 mixture of alfalfa and lespedeza and all lespedeza, respectively (ALF:LES and LES, respectively). There were four 6-wk periods, with digestion assessed in wk 4 and measures with a respiration calorimetry system in wk 5. Although there was a three-way interaction involving dietary treatment, breed, and period, average forage intake was greater for LES than for CON, ION, CCO, SBO (P < 0.05), and ALF:LES (P = 0.062; 988, 1072, 1265, 987, 951, and 1057 g/day for CON, ALF:LES, LES, ION, CCO, and SBO, respectively; standard error of the mean [SEM] = 69.4). Dietary level of lespedeza had marked effects (P < 0.05) on digestion of organic matter [OM] (69.2, 57.6, 50.3, 66.3, 66.0, and 68.7 %; SEM = 1.57) and nitrogen (78.3, 65.3, 46.8, 71.4, 73.9, and 75.7 %; SEM = 1.81), with digestible OM intake numerically lower (P > 0.05) for diets with lespedeza and for ION (697, 607, 589, 598, 635, and 690 g/day for CON, ALF:LES, LES, ION, CCO, and SBO, respectively; SEM = 50.4). There was a treatment by period interaction (P < 0.05) in ruminal methane emission relative to digestible energy intake, but overall, emission was greatest among treatments (P < 0.05) for CON (12.33, 9.92, 10.24, 10.41, 8.91, and 8.66 % for CON, ALF:LES, LES, ION, CCO, and SBO, respectively; SEM = 0.730). Average daily gain (ADG) was greatest among treatments (P < 0.05) for SBO (54, 28, 18, 40, 56, and 98 g; SEM = 13.2). Many findings are not closely aligned with those of previous studies with goats. For example, effects of lespedeza on feed intake and ruminal methane emission were greater in the first than second half of the experiment, though interactions observed could not be clearly attributed to factors such as microbial adaptation. The effect of the SBO diet on ADG with the alfalfa-based diet deserves future research attention.

Dietary intake of n-3 polyunsaturated fatty acids alters the lipid mediator profile of the kidney but does not attenuate renal insufficiency

The efficacy of n-3 polyunsaturated fatty acids (PUFAs) in improving outcomes in a renal ischemia-reperfusion injury (IRI) model has previously been reported. However, the underlying mechanisms remain poorly understood and few reports demonstrate how dietary n-3 PUFAs influence the composition of membrane phospholipids in the kidney. Additionally, it has not been elucidated whether perilla oil (PO), which is mainly composed of the n-3 alpha-linolenic acid, mitigates renal IRI. In this study, we investigated the effect of dietary n-3 PUFAs (PO), compared with an n-6 PUFA-rich soybean oil (SO) diet, on IRI-induced renal insufficiency in a rat model. Levels of membrane phospholipids containing n-3 PUFAs were higher in the kidney of PO-rich diet-fed rats than the SO-rich diet-fed rats. Levels of blood urea nitrogen and serum creatinine were significantly higher in the ischemia-reperfusion group than the sham group under both dietary conditions. However, no significant differences were observed in blood urea nitrogen, serum creatinine, or histological damage between PO-rich diet-fed rats and SO-rich diet-fed rats. In the kidney of PO-rich diet-fed rats, levels of arachidonic acid and arachidonic acid-derived pro-inflammatory lipid mediators were lower than SO-rich diet-fed rats. Eicosapentaenoic acid and eicosapentaenoic acid-derived lipid mediators were significantly higher in the kidney of PO-rich than SO-rich diet-fed rats. These results suggest that dietary n-3 PUFAs alter the fatty acid composition of membrane phospholipids and lipid mediators in the kidney; however, this does not attenuate renal insufficiency or histological damage in a renal IRI model.