Abstract
Acyl-CoA synthetase 6 (Acsl6) involves the enrichment of docosahexaenoic acid (DHA) and lipid synthesis in mammals, while its molecular function is still unknown in teleost. To explore the potential function of teleost Acsl6, this study investigated the molecular characterization and expression analysis of the acsl6 gene in the marine fish golden pompano (Trachinotus ovatus). The acsl6 cDNA cloned has a coding region (2169 bp) encoding 722 amino acids, which possesses a typical structure of Acsl including the gate domain and linker motif. The highest acsl6 mRNA expression was detected in the brain, followed in eyes, muscle, intestine, liver, and other tissues. Comparing to the fish fed with lard oil or soybean oil diets, the fish fed with fish oil exhibited high muscular DHA contents, and acsl6 mRNA levels (p < 0.05). Additionally, feeding T. ovatus three experimental diets containing different n-3 LC-PUFA levels (5.25%, 10.05%, and 14.05%) showed that the muscular DHA and n-3 LC-PUFA contents, as well as the acsl6 mRNA levels, matched the dietary n-3 LC-PUFA levels. The transcript level of acsl6 and contents of DHA in the muscle was significantly affected by dietary DHA/EPA ratios, and high DHA/EPA ratios increased the muscular DHA contents and acsl6 gene expression (p < 0.05). Moreover, the over-expression of acsl6 promoted the DHA contents of hepatocytes and enterocytes, suggesting that T. ovatus Acsl6 plays an active role in the deposition of DHA content. Collectively, the positive relationship among dietary DHA, muscular acsl6 mRNA and DHA levels, as well as the increase of cellular DHA contents by over-expression of acsl6, indicated that Acsl6 is beneficial to the DHA enrichment in T. ovatus. The acquisition of fish Acsl6 potential function in the present study will play the foundation for ameliorating the DHA contents in farmed fish products.
Published Version
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