Source Of Dietary Selenium Research Articles

Effects of Dietary Selenium Sources on Physiological Status of Laying Hens and Production of Selenium-Enriched Eggs.

Developing new sources of organic selenium (Se) has potential benefits for animal production and human nutrition via animal-based foods enriched with Se. The objective of this study was to evaluate the effects of Se-enriched insect protein (SEIP) in comparison with other sources, such as sodium selenite (SS) and selenium-enriched yeast (SEY), on performance, egg quality, selenium concentration in eggs, serum biochemical indices, immune capacity, and intestinal morphology of laying hens. Four hundred and fifty 24-week-old Hy-Line Brown laying hens with 94.0 ± 1.5% laying rate were randomly allocated to five groups with six replicates of 15 hens each. The control diet was prepared without adding exogenous selenium (calculated basal Se content of 0.08 mg/kg). The normal group was fed basal diets supplemented with 0.3 mg/kg of Se provided by sodium selenite. Three treatment groups (SS, SEY, and SEIP, respectively) were fed basal diets supplemented with 2 mg/kg of Se provided by sodium selenite, Se-enriched yeast, and SEIP, respectively. The feeding trial lasted for 12 weeks. Results revealed that dietary supplementation of 2 mg/kg of Se increased egg weight, decreased feed conversion ratio, and enhanced the antioxidant capacity of eggs in laying hens relative to the control group, whereas no significant differences were observed among SS, SEY, and SEIP treatment groups for the same. The organic source of Se provided by SEY or SEIP showed higher bio efficiency, as indicated by higher selenium content in eggs of SEY and SEIP compared with SS, although higher content was observed in SEY compared with SEIP. Also, the organic Se source significantly improved antioxidant capacity and immune functions of laying hens than the inorganic Se source. Diets supplemented with SEIP and SS significantly improved jejunal morphology of the laying hens compared with SEY, whereas SEIP was more effective than SEY to improve the oviduct health of laying hens. The results of this work evidently points the additive effect and nontoxicity of SEIP. Thus, SEIP could be used as another organic source of Se in the diet of laying hens and production of selenium-enriched eggs for humans.

Assessment of Iodine and Selenium Nutritional Status in Women of Reproductive Age in Latvia.

Background and Objectives: Adequate dietary intake of iodine and selenium is essential during pregnancy. While iodine is vital for maternal thyroid function and fetal development, selenium contributes to the regulation of thyroid function and thyroid autoimmunity. This study aimed to assess the consumption of iodine- and selenium-containing products by women of reproductive age and the iodine and selenium nutritional status of pregnant women in Latvia. Materials and Methods: Population health survey (2010–2018) data were used to characterize dietary habits in women of reproductive age. Additionally, 129 pregnant women in the first trimester were recruited; they completed a questionnaire and were tested for thyroid function, urinary iodine concentration (UIC), and serum selenium and selenoprotein P levels. Results: The use of some dietary sources of iodine (e.g., milk and dairy products) and selenium (e.g., bread) has decreased in recent years. Less than 10% of respondents reported the use of iodized salt. The use of supplements has become more common (reported by almost 50% of respondents in 2018). Dietary habits were similar in pregnant women, but the use of supplements was even higher (almost 70%). Nevertheless, most supplements used in pregnancy had insufficient contents of iodine and selenium. Thyroid function was euthyreotic in all women, but 13.9% of participants had a thyroid peroxidase antibodies (TPO-ab) level above 60 IU/mL. The median UIC (IQR) was 147.2 (90.0–248.1) μg/gCr, and 52.8% of pregnant women had a UIC below 150 μg/gCr. The mean selenium (SD) level was 101.5 (35.6) μg/L; 30.1% of women had a selenium level below 80 μg/L. The median selenoprotein P level was 6.9 (3.1–9.0) mg/L. Conclusions: Iodine nutrition in Latvian population of pregnant women was near the lower limit of adequate and a third of the population had a selenium deficiency. Supplements were frequently used, but most did not contain the recommended amounts of iodine and selenium.

Selenium Nanoparticles-loaded Chitosan Microspheres as a Dietary Selenium Source in Rabbits: Impact on Meat Selenium Content and Oxidative Stability

Selenium Nanoparticles-loaded Chitosan Microspheres as a Dietary Selenium Source in Rabbits: Impact on Meat Selenium Content and Oxidative Stability

Essential and toxic elements in sustainable and underutilized seafood species and derived semi-industrial ready-to-eat products

Blue whiting (Micromesistius poutassou), pouting (Trisopterus luscus) and common dab (Limanda limanda) are underutilized fish species attractive in terms of sustainability. However, there is limited information about their nutritional characteristics as well as potential presence of environmental contaminants. Specimens caught in European waters were characterised for their content of essential and toxic elements. The three species, along with common carp and blue mussels, relevant for sustainable production too, were employed as raw materials for the development of semi-industrial ready-to-eat products. Calcium, copper, iodine, iron, selenium, zinc, cadmium, lead, mercury, and nickel were determined by ICP-MS, whereas methylmercury was determined by HPLC-ICP-MS. These two techniques were also used to determine arsenic and inorganic arsenic, respectively, in blue mussel and derived products. Differences in element contents were related to the biology and ecology of the examined species. Intake of nutrients and exposure to contaminants were assessed in relation to the relevant DRVs and HBGVs, respectively. All the species were found to be valuable dietary sources of selenium. Pouting was rich in iodine and mussels were good sources of iodine and iron. These two species had comparatively higher levels of mercury and lead, respectively. However, the levels of contaminants were generally of no concern in both raw materials and products. Iodine bioaccessibility was studied in blue whiting, a species with an intermediate iodine content, and found to be 98%. Selenium:mercury molar ratios were assessed and found to be favourable. The semi-industrial products were found to be good sources of selenium and many of them provided appreciable amounts of calcium, iron, copper and zinc.

Dietary selenium sources differentially regulate selenium concentration, mRNA and protein expression of representative selenoproteins in various tissues of yellow catfish Pelteobagrus fulvidraco.

The study was conducted to determine the effects of three dietary Se sources, such as sodium-selenite (S-S), seleno-yeast (S-Y) and seleno-methionine (S-M), on Se concentration, glutathione peroxidase (GPX) and TXNRD activities, and mRNA expression of fifteen representative selenoproteins, and protein expression of four endoplasmic reticulum-resided selenoproteins in a wide range of tissues of yellow catfish. Compared with S-S and S-M groups, dietary S-Y significantly decreased growth performance and feed utilisation of yellow catfish. Dietary Se sources significantly influenced Se contents in the spleen, dorsal muscle and the kidney, GPX activities in spleen, kidney, intestine, muscle and mesenteric fat, and TXNRD activities in the heart, intestine and mesenteric fat. Among ten tested tissues, dietary Se sources influenced mRNA expression of GPX4 and SELENOK in three tissues; GPX3, SELENOS and TXNRD2 in four tissues; SELENOF, SELENON and DIO2 in five tissues; SELENOM, GPX1/2 and TXNRD3 in six tissues; SELENOW in seven tissue and SELENOP and SELENOT in eight tissues. Based on these observations above, S-S and S-M seem to be suitable Se sources for improving growth performance and feed utilisation of yellow catfish. Dietary Se sources differentially influence the expression of selenoproteins in various tissues of yellow catfish. For the first time, we determined the expression of selenoproteins in fish in responses to dietary Se sources, which contributes to a better understanding of the functions and regulatory mechanisms of selenoporteins.

Geopedology-climate interactions govern the spatial distribution of selenium in soils: A case study in northeastern Brazil

Selenium (Se) is an essential trace element in humans with critical roles in immune functioning and antioxidant systems. Plant foods growing on soils with sufficient Se levels are the primary dietary sources of selenium. Therefore, knowing the factors that drive the concentration of Se in soils is essential to map Se deficiencies and toxicities and implement biofortification programs to enhance foodstuffs' mineral composition. Here, we assessed how geopedology-climate interactions govern the background concentration and spatial distribution of Se in soils from two northeastern Brazil states. A soil geochemical survey covering roughly 110,000 km2 collected 198 composite samples in sites with minimal anthropogenic influence and wide variation concerning soil types, geological and climatological settings. The samples had their Se concentrations determined by hydride generation atomic absorption spectroscopy (HGAAS) after microwave acid digestion. The results indicate that climate (precipitation) and bedrock are the main broad-scale factors on regional soil Se distribution, while soil contents of clay and organic matter influence Se values locally. Geographically weighted regression (GWR) showed that these factors change over space and exist in a spatial non-stationarity relationship with selenium. Se-deficient soils are most likely to occur in semi-arid settings overlying crystalline rocks, in which pedogenesis and climate drive low contents of clay and soil organic carbon, and Se air-borne inputs are low. The risk of inadequate Se intake by humans and animals in the region is high. This study can aid public policies addressing Se malnutrition in the study region and similar geopedological and climate settings worldwide.

Selenium nanoparticle and selenomethionine as feed additives: effects on growth performance, hepatic enzymes' activity, mucosal immune parameters, liver histology, and appetite-related gene transcript in goldfish (Carassius auratus).

The aim of this study was to assess the effects of different dietary selenium sources, selenium nanoparticle (nSe), and selenomethionine (SeMet) as feed additives on growth performance, hepatic enzymes' activity, biochemical, mucosal immune parameters, liver histology, and appetite-related gene transcript in goldfish (Carassius auratus). At first, goldfish juveniles (n=480; mean 4.54 g) were fed dietary selenium nanoparticle at 0, 0.3, 0.6, and 0.9 mg nSe/kg diet and SeMet at 0, 0.3, 0.6, and 0.9 mg Se/kg for 9 weeks. Growth performance was evaluated using standard procedures. Blood, skin mucus, and tissue samples (liver and intestine) were collected for biochemical, mucosal immune response, histology, and ghrelin and insulin-like growth factor-I (IGF-I) gene expression. The results showed that fish fed diets fortified with 0.6 mg nSe/kg and 0.6 mg Se/kg had a significant higher weight gain, specific growth rates (SGR), and lower feed conversion ratios (FCR) than fish fed basal diets (p<0.05). Furthermore, dietary nSe and SeMet enhanced blood biochemical profiles especially alkaline phosphatase (ALP) (p < 0.05) and mucosal immunity than the control group in goldfish. Moreover, the liver histological investigation showed that fish fed 0.9 mg of SeMet and nSe kg-1 diets had higher liver lesion scores such as karyolysis, lipidosis, and hyperemia while fish fed 0, 0.3, and 0.6 mg of SeMet and nSe kg-1 diets had small liver changes at 9 weeks. The study further established that inclusion of nSe and SeMet in the diet of goldfish greatly promoted ghrelin and IGF-1genes expressions (p <0.05). Overall, dietary nSe performs better than SeMet and basal diets. The results evoked that nSe and SeMet stimulate the growth, biochemical, and mucosal immunity in goldfish at 0.6 mg/kg.

Revisiting the Effects of Different Dietary Sources of Selenium on the Health and Performance of Dairy Animals: a Review.

Selenium (Se) is one of the most important essential trace elements in livestock production. It is a structural component in at least 25 selenoproteins such as the iodothyronine deiodinases and thioredoxin reductases as selenocysteine at critical positions in the active sites of these enzymes. It is also involved in the synthesis of the thyroid hormone and influences overall body metabolism. Selenium being a component of the glutathione peroxidase enzyme also plays a key role in the antioxidant defense system of animals. Dietary requirements of Se in dairy animals depend on physiological status, endogenous Se content, Se source, and route of administration. Most of the dietary Se is absorbed through the duodenum in ruminants and also some portion through the rumen wall. Inorganic Se salts such as Na-selenate and Na-selenite have shown lower bioavailability than organic and nano-Se. Selenium deficiency has been associated with reproductive disorders such as retained placenta, abortion, early embryonic death, and infertility, together with muscular diseases (like white muscle disease and skeletal and cardiac muscle necrosis). The deficiency of Se can also affect the udder health particularly favoring clinical and subclinical mastitis, along with an increase of milk somatic cell counts in dairy animals. However, excessive Se supplementation (5 to 8 mg/kg DM) can lead to acute toxicity including chronic and acute selenosis. Se is the most vital trace element for the optimum performance of dairy animals. This review focuses to provide insights into the comparative efficacy of different forms of dietary Se (inorganic, organic, and nano-Se) on the health and production of dairy animals and milk Se content.

The mixed application of organic and inorganic selenium shows better effects on incubation and progeny parameters

This experiment aims to study the effects of dietary selenium (Se) sources on the production performance, reproductive performance, and maternal effect of breeder laying hens. A total of 2,112 Hyline brown breeder laying hens of 42 wk of age were selected and randomly divided into 3 groups, with 8 repeats in each group and 88 chickens per repeat. The sources of dietary Se were sodium selenite (SS, added at 0.3 mg/kg), L-selenomethionine (L-SM, added at 0.2 mg/kg), and combination of SS and L-SM (SS 0.15 mg/kg + L-SM 0.15 mg/kg). The pretest period was 7 d, and the breeding period was 49 d. Compared with 0.3 mg/kg SS, the addition of 0.2 mg/kg L-SM in the diet significantly increased the hatchability (P < 0.05) and the Se content (P < 0.05) in egg yolk and chicken embryo tissues and improved the activity of yolk glutathione peroxidase (GSH-px) effectively (P < 0.05). Treatment with 0.2 mg/kg L-SM also reduced the content of yolk malondialdehyde (P < 0.05) and significantly improved the antioxidant performance of 1-day-old chicks, as manifested by increased activity of antioxidant enzymes (GSH-px, total antioxidant capacity and the ability to inhibit hydroxyl radicals) in serum, pectoral, heart, and liver (P < 0.05). This treatment decreased the malondialdehyde content (P < 0.05) and increased the expression of liver glutathione peroxidase 4 and deiodinase 1 mRNA (P < 0.05). Adding L-SM to the diets of chickens increased the hatchability of breeder eggs as well as the amount of Se deposited and antioxidant enzyme activity in breeder eggs and embryos. Compared with SS, L-SM was more effectively transferred from the mother to the embryo and offspring, showing efficient maternal nutrition. For breeder diets, the combination of organic and inorganic Se (0.15 mg/kg SS + 0.15 mg/kg L-SM) is an effective nutrient supplementation technology program for effectively improving the breeding performance of breeders and the antioxidant performance and health level of offspring chicks.

Effect of source and concentration of dietary selenium on laying performance of egg-type breeders and antioxidant status of their offspring under delayed post- hatch feeding

Abstract The present study aimed to evaluate the effect of dietary organic or inorganic selenium (Se) supplementation on laying and hatching performance, egg Se content and liver glutathione peroxidase (GSH-Px) concentration of egg-type breeders as well as their offspring antioxidant status under 36Â h post-hatch feed withdrawal. Two hundred and seventy white egg-type breeders were used. The hens were randomly assigned to five dietary treatments. The basal diet contained no supplemental Se (Control). The four treatment groups consisted of two selenium sources (inorganic and organic, Se inorg and Se org, respectively) and two selenium levels (0.20 and 0.35Â mg Se/kg diet). The total analysed Se concentration in the diets was between 0.137 and 0.138Â mg/kg for Control, while it was 0.342 and 0.340Â mg/kg for 0.20 Se inorg, 0.339 and 0.351Â mg/kg for 0.20 Se org, 0.485 and 0.489Â mg/kg for 0.35 Se inorg, 0.481 and 0.495Â mg/kg for 0.35 Se org from 21 to 40 and 41 to 49 weeks, respectively. There was no effect of diets on laying performance, egg quality, and hatchability. Dietary Se supplementation increased egg Se content compared to Control, being higher in eggs from hens fed Se org than those from hens fed Se inorg. Liver GSH-Px activity of hens fed 0.35Â mg Se/kg was higher than in hens fed 0.20Â mg/kg Se. On d of hatch and 36Â h post-hatch, residual yolk sac Se content of chicks from hens fed 0.35Â mg Se/kg was higher than that of chicks from hens fed 0.20Â mg/kg Se. After 36Â h post-hatch feed withdrawal, plasma and liver GSH-Px activity of chicks from Se groups were higher than in chicks from the Control. It was concluded that dietary maternal Se would provide a protective mechanism for day-old chicks for coping with stressful situations exposed after hatching.

Supplemental hot melt extruded nano-selenium increases expression profiles of antioxidant enzymes in the livers and spleens of weanling pigs

A study was conducted to determine the effect of dietary selenium (Se) concentration and source for weanling pigs on serum Se, glutathione peroxidase (GPx) activity and performance. A total of 120 weanling pigs with an average weight of 6.87 ± 0.4 kg were assigned to 4 diets with 10 pigs per cage and 3 replicates per treatment. The experimental diets were fed for 28 days in 2 phases (phase 1, d 0–14; and phase 2, d 15–28). Treatments were: Control (without Se supplementation), sodium selenite (INO; 0.30 ppm), hot melt extruded sodium selenite (HME; 0.30 ppm), and selenomethionine (ORG; 0.3 ppm). There were significant responses (P < 0.05) for higher serum Se concentration in the HME and ORG treatments in phase 1 and 2. The serum GPx analyses revealed that supplemental HME and ORG significantly increased the concentration of GPx in the serum in phase 1 and phase 2. There was an up-regulated (P < 0.01) expression of Selenoprotein W, GPx1, GPx3, GPx4, and GPx5 in the livers of the HME and ORG pigs. In conclusion, the HME and ORG Se showed increased antioxidant status, consequently being a more suitable source of Se than regular sodium selenite.

Selenium Status and Hemolysis in Sickle Cell Disease Patients.

Sickle cell disease (SCD) is a genetic hemoglobinopathy characterized by chronic hemolysis. Chronic hemolysis is promoted by increased oxidative stress. Our hypothesis was that some antioxidant micronutrients (retinol, tocopherol, selenium, and zinc) would be determinant factors of the degree of hemolysis in SCD patients. We aimed to investigate the nutritional adequacy of these antioxidants and their relationships to hemolysis. The study included 51 adult SCD patients regularly assisted in two reference centers for hematology in the State of Rio de Janeiro, Brazil. Serum concentrations of retinol, alpha-tocopherol, selenium, and zinc were determined by high-performance liquid chromatography or atomic absorption spectrometry. Hematological parameters (complete blood count, reticulocyte count, hemoglobin, direct and indirect bilirubin, total bilirubin, lactate dehydrogenase) and inflammation markers (leukocytes and ultra-sensitive C-reactive protein) were analyzed. A linear regression model was used to test the associations between the variables. Most patients presented selenium deficiency and low selenium consumption. Linear regression analysis showed that selenium is the main determinant of hemolysis among the antioxidant nutrients analyzed. Thus, data from this study suggest that the nutritional care protocols for patients with SCD should include dietary sources of selenium in order to reduce the risk of hemolysis.

Usual intake and dietary sources of Selenium in adolescents: A cross-sectional school-based study

Selenium is a mineral that constitutes selenoproteins and, therefore, has been studied, especially in cardiovascular diseases. Some risk factors for the development of these diseases, such as obesity, hypertension and dyslipidemia, have been observed early in life, including in childhood and adolescence, and food and nutrient intake is an important associated factor for their development. The aim of this study was to assess usual intake of selenium (Se) and dietary inadequacies regarding the consumption of this mineral, and to identify the main dietary sources of Se among 12-17 year-old Brazilian adolescents. Data from the Study of Cardiovascular Risk in Adolescents, performed countrywide in the years 2013 and 2014, were used to estimate inadequate Se dietary intake. The National Cancer Institute's method was used, considering two days of 24h dietary recall (24hR). The contribution of each food group to the total intake of Se was determined using a single 24hR, and was defined as a ratio between the total intake of Se and the percentage of Se in each food group, until reaching 95%. Food mentioned by adolescents were categorized into 39 food groups, according to macronutrient similarities. Data from 76,957 adolescents aged from 12-17 years (49.7% girls) from public and private schools were assessed. Selenium intake was according to recommendation levels. Mean Se intake ranged from 84.3 to 105.9μg among sex and age groups. Considering the whole sample, meat, pasta, poultry, and fish were the food groups that contributed with the greatest amount of Se (representing 57.9% of total Se). Brazil nuts are consumed in bigger quantity only by 14-17 year-old girls, contributing to 1.6% of the Se in their diets. Brazilian adolescents have an adequate Se intake because of the high consumption of meat, pasta, and poultry. Although Brazil nuts are the most important dietary source of Se, adolescents consume them in very small amounts, leading to a small contribution in the total intake.

Biological Evaluation of Hot-Melt Extruded Nano-selenium and the Role of Selenium on the Expression Profiles of Selenium-Dependent Antioxidant Enzymes in Chickens.

A study was conducted to determine the effect of dietary selenium (Se) concentration and source for broiler chickens on performance, nutrient digestibility, plasma Se, glutathione peroxidase (GPx) activity, and thiobarbituric acid reactive substances (TBARS). A total of 700 1-day-old broiler chicks were assigned to 7 diets with 20 birds per cage and 5 replicates per treatment. The experimental diets were fed for 32days in 2 phases (phase 1, day 0 to 14 and phase 2, day 15 to 32). Treatments were as follows: control (without Se supplementation), sodium selenite (SeS; 0.15, 0.30, or 0.45ppm), and hot-melt extruded sodium selenite (SeHME; 0.15, 0.30, or 0.45ppm). There were significant linear responses (P< 0.01) for higher plasma Se concentration in SeS and SeHME treatments. Moreover, an increased (P< 0.01) Se concentration of plasma occurred in SeHME treatment compared with that in SeS treatment. The serum GPx analyses revealed that supplemental SeS and SeHME increased significantly the activity of GPx in the plasma in phase 1 (P< 0.05) and phase 2 (P< 0.05). There were significant linear (P< 0.01) responses of SeS and SeHME treatments for the expression of SelW, GPx1, GPx3, and GPx4 in the livers and spleens. In addition, SeHME showed an upregulated expression of GPx-4 in the livers (P< 0.01) and SelW in the spleens (P< 0.05) compared with SeS treatment. SeHME showed a lower TBARS on day 9. Moreover, a decreased (P< 0.01) TBARS occurred in SeS treatment compared with that in control treatment. In conclusion, SeHME can increase antioxidant activity and Se absorption, consequently being a more suitable source of Se than regular sodium selenite.

Effects of Dietary Selenium Sources on Metabolic, Enzymatic and Immunoglobulin Serum Profiles in Growing Rabbits

Effects of Dietary Selenium Sources on Metabolic, Enzymatic and Immunoglobulin Serum Profiles in Growing Rabbits

Effects of different dietary selenium sources on growth performance, liver and muscle composition, antioxidant status, stress response and expression of related genes in gilthead seabream (Sparus aurata)

The present study aimed to determine the effects of dietary inclusion of selenium (Se) in the form of either inorganic Se (sodium selenite, NaSe) or organic Se (hydroxy-selenomethionine, OH-SeMet) on gilthead seabream (Sparus aurata). Triplicate groups of 150 fish each (6.2 ± 0.04 g initial body weight) were distributed in 15 tanks and fed one of five experimental diets for 63 days. A control diet (C) without Se supplementation (0.8 mg Se/kg diet), was formulated and served as a basal diet for the other 4 experimental feeds, supplemented with either 0.2 or 0.5 mg Se/kg supplied in the form of NaSe or OH-SeMet. Fish growth performance, biochemical composition of liver and muscle and Se content, malondialdehyde (MDA) concentration in muscle and hepatopancreas, hepatocyte morphology and lysozyme activity in the serum were studied. Furthermore, expression of related hepatic genes, such as manganese superoxide dismutase (Mn-sod), catalase (cat), heat shock protein 70 (hsp70), and glutathione peroxidase (gpx) was also analyzed. After the trial, fish were exposed to an acute and chronic confinement stress. Blood samples for cortisol analysis were taken at 0 and 2 h after the acute stress and after 7 days of chronic stress.The highest growth rate was observed in fish fed OH-SeMet at a level of 0.2 mg/kg, but with no significant difference with fish fed the control diet with no-added Se. The lowest growth was observed in fish fed NaSe up to 0.5 mg/kg. Increase in dietary Se, particularly in the form of OH-SeMet, led to an increase in Se contents in liver and muscle. Furthermore, dietary inclusion of OH-SeMet, led to a significant (p < .05) reduction in MDA in both liver and muscle. Se inclusion as NaSe at 0.2 mg/kg was not as effective as organic Se to improve muscle oxidative status. Dietary inclusion of Se at 0.2 mg/kg significantly reduced plasma cortisol after 2 h of acute stress, regardless of the Se form fed. Lysozyme activity measured in the serum was decreased with the increase in dietary Se supplementation.In summary, supplementation of Se up to 0.2 mg/kg (1–1.1 mg/kg analyzed dietary Se), particularly in the form of OH-SeMet, led to a beneficial effect on growth, maintenance of hepatic morphology and improved protection of juvenile gilthead seabream from acute or chronic stress. Besides, OH-SeMet was found to be more effective than NaSe in protection against oxidative stress in fish muscle.

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Chickpea (Cicer arietinum L.) sprouts containing supranutritional levels of selenium decrease tumor growth of colon cancer cells xenografted in immune-suppressed mice

The effect of selenium enriched chickpea sprouts with and without isoflavonoids (IS) on colorectal cancer (CRC) tumor growth was evaluated in immune-suppressed mice xenografted. Two levels of selenium (0.17 and 2.29 µg/g) and isoflavonoids (0 and 2.34 mg/g) were evaluated in the diets. High intake of Se (2.29 µg/g) with and without isoflavonoids increased glutathione peroxidase (GPx) and tioredoxin reductase (TrxR) activities, cholesterol (CHOL), triglycerides (TRIGL) and low-density lipoprotein cholesterol (LDL-C) levels and decreased significantly the tumor growth. Diet containing selenium at 2.29 µg/g and isoflavonoids at 2.34 mg/g levels promoted apoptosis through the overexpression of Fas cell surface death receptor (Fas). High selenium levels in chickpea sprouts exert chemopreventive effects mediated mainly by the antioxidant protection of glutathione peroxidase. Selenized chickpea sprouts represent an excellent source of dietary selenium and isoflavonoids with chemopreventive potential and could be potentially used in the treatment of colorectal cancer.

Selenium speciation and biological characteristics of selenium-rich Bailing mushroom, Pleurotus tuoliensis

Nowadays the study of selenium-rich mushrooms is very popular. In the present study, selenium speciation in fruiting body of Pleurotus tuoliensis was investigated in cultivation substrates with different concentrations of sodium selenite, as well as mycelia growth and mushroom development. The results showed that the P. tuoliensis mycelia appeared good tolerance to selenium at all test concentrations. A selenium concentration of 10 mg/kg promoted fruiting of P. tuoliensis; the fruiting bodies were of good quality and had a low malformation rate. HPLC–ICP-MS determined that organic seleniums enriched in stipes and caps existed mainly in the form of selenoCystine and selenoMethionine at selenium concentrations of 10-100 mg/kg. These findings suggest that P. tuoliensis could be developed as a selenium-rich mushroom product for use as a novel dietary source of bioavailable supplemental selenium.

Effects of dietary organic, inorganic, and nanoparticulate selenium sources on growth, hemato-immunological, and serum biochemical parameters of common carp (Cyprinus carpio).

An 8-week feeding trial was conducted to compare the effects of supplementing (0.7mgkg-1) different dietary selenium (Se) sources including organic [selenomethionine (SeMet)], inorganic [sodium selenite (Na2SeO3)], and nanoparticulate Se (nano-Se) on physiological responses of common carp, Cyprinus carpio juveniles (9.7 ± 0.1g). Basal diet without Se supplementation used as control. Fish fed nano-Se supplemented diet had the highest weight gain (97.2 ± 10.8%) and feed efficiency ratio (42.4 ± 0.8%). Intestinal villi height was significantly taller in fish fed nano-Se diet than in the control group in both foregut and midgut sections. Serum glutathione peroxidase and superoxide dismutase activities were significantly higher in nano-Se and SeMet groups than in control and sodium selenite groups. Fish fed Se-supplemented diets had greater red blood cell counts and hematocrit and hemoglobin values than the control group (P < 0.05). Nano-Se and SeMet groups showed a significant increase in white blood cell counts, neutrophil percentage, and serum lysozyme activity than the other groups. Fish fed nano-Se diet had the highest serum hemolytic activity, total immunoglobulin, and total protein and albumin contents, as well as the lowest serum total cholesterol and low density lipoprotein levels (P < 0.05). Overall, significant improvements in growth performance, feed utilization, intestinal morphology, and hemato-immunological and serum biochemical parameters of common carp juveniles suggest nano-Se as an efficient source for providing dietary Se in this species.