Dietary Macronutrient Intake Research Articles

The association of energy or macronutrient intake in three meals with depression in adults with cardiovascular disease: the United States National Health and Nutrition Examination Survey, 2003-2018.

There is growing evidence that individuals with cardiovascular disease (CVD) are more likely to develop depression.The timing of food intake can significantly alter the body's circadian rhythm and affect the occurrence of depression. Currently, it is unknown whether and how energy or macronutrient intake times are associated with depression in adults with CVD. To evaluate dietary energy or macronutrient intake (across three meals) associations with depression in adults with CVD in a nationally representative sample. The study population consisted of 3,490 U.S. adults with CVD (including 554with depression) from the National Health and Nutrition Examination Survey 2003-2018. Energy and macronutrient intake was measured by a 24-h dietary recall, and depression was diagnosed by the Patient Health Questionnaire (PHQ-9, score ≥ 10). According to dietary energy or macronutrient intake across three meals, adults with CVD were divided into five groups. Logistic regression analysis was performed to examine associations between energy or macronutrient intake and depression after adjusting for a series of confounding factors,including age, gender, education level, household income, smoking status, drinking status, physical activity, marital status, skipping breakfast/lunch/dinner, total energy, carbohydrate, protein,dietary fiber, SFA, MUFA, and PUFA intake, T2DM and hypertension status, and BMI. Dietary substitution models were used to explore changes in depression risk when 5% dietary energy intake at dinner or lunch was substituted with energy intake at breakfast. When compared with participants in the lowest quintile of breakfast energy intake, those who received energy intake in the highest quintile at breakfast were associated with lower depression risk in those with CVD, and the adjusted odds ratio (OR) was0.71 (95% CI, 0.51 to 0.91).When compared with participants in the lowest quintile of lunch or dinner energy intake, the risk of depression did not exhibit statistical significance when lunch or dinner energy intake was in the highest quintile, and the adjusted ORswere1.08 (95% CI,0.65 to 1.83) and 0.92(95% CI,0.62to1.37), respectively. Isocalorically replacing 5% of total energy at dinner or lunch with breakfast was associated with 5% (OR: 0.95, 95% CI 0.93 to 0.97) and 5% (OR: 0.95, 95% CI 0.93 to 0.96) lower risk of depression, respectively. High energy intake at breakfast may be associated with a lower risk of depression in those with CVD.We should focus on the potential role of breakfast energy intake in preventing the onset of depression.

The effects of seven-strain probiotic supplementation on cell adhesion molecules, oxidative stress, and antioxidant parameters in patients with traumatic brain injury: a randomized controlled clinical trial.

The therapeutic effects of probiotics in patients with traumatic brain injury (TBI) remain unclear. This study aimed to investigate the effects of probiotic supplementation on cell adhesion molecules, oxidative stress, and antioxidant parameters in TBI patients. This randomized, double-blind, placebo-controlled trial included 46 TBI patients who were randomly assigned to receive either a probiotic supplement (n = 23) or a placebo (n = 23) for 14 days. The probiotic capsule contained four strains of Lactobacillus (L. casei, L. bulgaricus, L. rhamnosus, L. acidophilus), two strains of Bifidobacterium (B. longum, B. breve), and Streptococcus thermophilus. Serum levels of intercellular adhesion molecule (ICAM)-1, vascular cell adhesion molecule (VCAM)-1, pro-oxidant antioxidant balance (PAB), malondialdehyde (MDA), nitric oxide (NO), total antioxidant capacity (TAC), and arylesterase (ARE) activity were measured at the beginning and end of the trial. Dietary intakes of patients were also recorded at the beginning and end of the trial. At the end of the study, there were no significant changes in ICAM-1, VCAM-1, PAB, MDA, NO, TAC, and ARE levels. However, patients who received probiotic supplements had significantly increased dietary intakes of energy, macronutrients, vitamin E, zinc, copper, and selenium compared with the placebo group. This study provides evidence that probiotic supplementation for 14 days in TBI patients has beneficial effects on dietary intake. However, it did not affect serum levels of cell adhesion molecules, oxidative stress, or antioxidant parameters. These findings should be considered preliminary, and further research is needed to evaluate long-term and clinical outcomes.

Interaction between genetic risk score and dietary carbohydrate intake on high-density lipoprotein cholesterol levels: Findings from the study of obesity, nutrition, genes and social factors (SONGS).

Cardiometabolic traits are complex interrelated traits that result from a combination of genetic and lifestyle factors. This study aimed to assess the interaction between genetic variants and dietary macronutrient intake on cardiometabolic traits [body mass index, waist circumference, total cholesterol, high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol, triacylglycerol, systolic blood pressure, diastolic blood pressure, fasting serum glucose, fasting serum insulin, and glycated haemoglobin]. This cross-sectional study consisted of 468 urban young adults aged 20±1 years, and it was conducted as part of the Study of Obesity, Nutrition, Genes and Social factors (SONGS) project, a sub-study of the Young Lives study. Thirty-nine single nucleotide polymorphisms (SNPs) known to be associated with cardiometabolic traits at a genome-wide significance level (P<5×10-8) were used to construct a genetic risk score (GRS). There were no significant associations between the GRS and any of the cardiometabolic traits. However, a significant interaction was observed between the GRS and carbohydrate intake on HDL-C concentration (Pinteraction=0.0007). In the first tertile of carbohydrate intake (≤327g/day), participants with a high GRS (>37 risk alleles) had a higher concentration of HDL-C than those with a low GRS (≤37 risk alleles) [Beta=0.06mmol/L, 95% confidence interval (CI), 0.01-0.10; P=0.018]. In the third tertile of carbohydrate intake (>452g/day), participants with a high GRS had a lower concentration of HDL-C than those with a low GRS (Beta=-0.04mmol/L, 95% CI -0.01 to-0.09; P=0.027). A significant interaction was also observed between the GRS and glycaemic load (GL) on the concentration of HDL-C (Pinteraction=0.002). For participants with a high GRS, there were lower concentrations of HDL-C across tertiles of GL (Ptrend=0.017). There was no significant interaction between the GRS and glycaemic index on the concentration of HDL-C, and none of the other GRS∗macronutrient interactions were significant. Our results suggest that young adults who consume a higher carbohydrate diet and have a higher GRS have a lower HDL-C concentration, which in turn is linked to cardiovascular diseases, and indicate that personalised nutrition strategies targeting a reduction in carbohydrate intake might be beneficial for these individuals.

Role of the Unique Secreted Peptide Adropin in Various Physiological and Disease States.

Adropin, a secreted peptide hormone identified in 2008, plays a significant role in regulating energy homeostasis, glucose metabolism, and lipid metabolism. Its expression is linked to dietary macronutrient intake and is influenced by metabolic syndrome, obesity, diabetes, and cardiovascular diseases. Emerging evidence suggests that adropin might be a biomarker for various conditions, including metabolic syndrome, coronary artery disease, and hypertensive disorders complicating pregnancy. In cerebrovascular diseases, adropin demonstrates protective effects by reducing blood-brain barrier permeability, brain edema, and infarct size while improving cognitive and sensorimotor functions in ischemic stroke models. The protective effects of adropin extend to preventing endothelial damage, promoting angiogenesis, and mitigating inflammation, making it a promising therapeutic target for cardiovascular and neurodegenerative diseases. This review provides a comprehensive overview of adropin's multifaceted roles in physiological and pathological conditions, as well as our recent work demonstrating adropin's role in subarachnoid hemorrhage-mediated neural injury and delayed cerebral infarction.

The relationship between low-carbohydrate diet score, dietary macronutrient intake, and rheumatoid arthritis: results from NHANES 2011-2016.

This study sought to determine if dietary macronutrient consumption and the low-carbohydrate diet (LCD) score were linked to rheumatoid arthritis (RA). Participants ≥ 20years were analyzed from the National Health and Nutrition Examination Survey (NHANES) 2011-2016. LCD score was calculated by summing the 11 quantiles values of the percentages of energy derived from carbohydrate, protein, and fat. Weighted logistic regression, eXtreme Gradient Boosting (XGBoost), and Light Gradient Boosting Machine (LightGBM) models were used to explore the relationship between LCD score, dietary macronutrient intake, and RA. Propensity score matching (PSM) were applied for sensitivity analysis. Ultimately, 8118 participants (RA: 499, without RA: 7619) were analyzed. After fully adjusting for confounders, a negative association was found between the LCD score and the presence of RA [OR (95% CI), 0.97 (0.96, 0.99)]. A higher LCD score was also negatively associated with a lower likelihood of RA based on a categorical model. Among macronutrients, participants in the third and fourth quartiles had significantly increased odds of RA compared with the lowest carbohydrate intake. Regarding protein intake, individuals in the highest quartile of percentage of energy from protein had a 46% lower presence of RA compared with the lowest reference group. The relative importance of the LCD score on RA was determined based on XGBoost and LightGBM models. Moreover, the association between the LCD score, dietary macronutrient intake, and RA presence remained substantial after PSM. LCD score was negatively associated with odds of RA in US adults. Moreover, a correlation was found between a lower likelihood of RA and high protein, and low carbohydrate consumption. Key Points • A significant negative association was found between LCD score and RA presence. • Machine learning models revealed the LCD score was a significant predictor of the presence of RA. • Low carbohydrate intake and high protein intake were correlated with a lower odds of RA.

Racial and Ethnical Disparities in Dietary Intakes Among Adults with Diagnosed Diabetes: National Health and Nutrition Examination Survey 2011-2020

Backgrounds: Diet holds crucial significance in the management and prevention of type 2 diabetes. This study aims to compare dietary intake of key macronutrients and micronutrients across different race/ethnicity among adults with diabetes.Method: A cross-sectional analysis was conducted in 3,120 adults with diagnosed diabetes using the NHANES data from 2010 to March 2020. Type 2 diabetes was defined based on self-reported diagnosis or the use of anti-diabetic medications. Participants diagnosed with diabetes at age < 25years were assumed to have type 1 diabetes and were excluded. Race/ethnicity were categorized into White, Black, Asian, Mexican and other Hispanic adults. We compared the energy-adjusted nutrient intakes by race/ethnicity group and adjusted for sex and age.Results: There were significant differences in various nutrient intakes across race/ethnicity in adults with diabetes . Compared to White adults, Black adults were less likely to consume saturated fatty acids, calcium, zinc, and potassium; Asian adults had higher carbohydrate, fiber, magnesium, and sodium intakes but lower sugar, fat, saturated fatty acids, cholesterol, and calcium consumptions; Mexican adults had higher fiber and magnesium intakes but a lower sugar intake; other Hispanic adults had lower intakes of total fat, saturated fatty acids and monounsaturated fatty acids.(all p<0.01.)Conclusion: There were significant racial/ethnical differences in dietary intake among various racial and ethnic groups in the US population. Tailored approaches through racial and ethnic considerations may

Abstract 4119926: Inadequate calorie and macronutrient intake in a population with congestive heart failure in a non-cardiovascular tertiary hospital.

Introduction: Chronic Heart Failure (HF) is a clinical syndrome that as a consequence of the compensatory mechanisms to maintain an adequate level of perfusion, causes dyspnea, peripheral edema, fatigue, lack of appetite, changes in body weight, cachexia, sarcopenia, among others. The presence of these signs and symptoms, with other factors such as restrictions on salt and water consumption, early satiety, and poor mobility, producing changes in the patient's nutritional status that can indirectly increase the risk of inadequate dietary intake, which over a prolonged period will lead to the development of nutritional deficiencies. Objective: To analyze the dietary intake of energy, macronutrients, and micronutrients of patients with chronic HF in outpatient care. Methods: Cross-sectional study, including 75 outpatients with HF, who attended a Heart Failure Clinic. Data collection on dietary intake was obtained using a multi-step 24-hour recall. The ESHA Food Processor program was used to calculate the energy, macronutrient, and micronutrient intake. The presence of congestion was evaluated by bioelectrical impedance vectorial analysis and VEXUS. Results: The baseline characteristics of the population are shown in Table 1. Of the total population, 34% presented with deficient caloric intake, and the subjects who presented congestion also ingested significant fewer calories compared with those who do not present congestion (p= 0.031). Only 15% of patients had an adequate protein intake that resulted in lower NYHA class; among those subjects with a deficient protein intake, 72% had congestion measured by VExUS or bioelectrical impedance. For the sodium intake in the subjects' diet, 45% exceeded the recommendation stated in the guidelines of less than 2,400 mg of sodium per day which was not statistically significant (p=0.077). Discussion and Conclusions: The inadequate intake of calorie or macronutrients in patients with CHF led the patients to more congestion and higher NYHA class, this provide a perspective on the importance of adequate nutritional advice by an expert in the area, which is also essential to improve the clinical course of heart failure. Likewise, future studies are deeded to investigate whether this poor dietary intake is inversely related to some clinical manifestations such as congestion, suggesting that improving these conditions could positively impact patients' volume status and improve functional class.

Short-term high-fat and high-carb diet effects on glucose metabolism and hedonic regulation in young healthy men.

Daily dietary intake of macronutrients and energy is closely associated with long-term metabolic health outcomes, but whether 24-h nutritional intervention under isocaloric conditions leads to changes in metabolism remains unclear. Moreover, the short-term effect of diets with different macronutrient composition on hedonic appetite regulation is less clear. This study examined the impact of an acute high-fat (F+) and high-carbohydrate (C+) diet on glucose metabolism and hedonic regulation of food intake in young healthy men under controlled conditions. Using a cross-over design, 19 male participants received a one-day isocaloric diet with different macronutrient composition (F+ = 11% carbohydrates, 74% fat; C+ = 79% carbohydrates, 6% fat) compared to a control diet (CON = 55% carbohydrates, 30% fat). Protein content was set at 15% of energy in all diets. The feeling of hunger, as well as "liking" and "wanting" for foods, was assessed through visual analog scales, and blood samples for glucose, insulin, and cortisol levels were assessed repeatedly during the experimental day. An intravenous glucose tolerance test was conducted the next morning. Postprandial glucose and insulin levels were lowest in F+ over the 24 h. Except for dinner, the CON diet showed the highest mean values in glucose. F+ diet improved insulin resistance, lowering Homeostatis Model Assessment Insulin Resistance (HOMA-IR) values. Changes in hedonic regulation of food intake were not observed during the intervention between the diets, except for higher feelings of satiety under the CON diet. An acute, isocaloric, high-fat diet improved insulin resistance even in healthy individuals but did not affect hedonic food intake regulation. Macronutrient composition modulate glucose metabolism even under short-term (24-h) and isocaloric diets, which should be considered for personalized preventive dietary treatments.

Dietary Macronutrient and Micronutrient Intake Among Corporate Employees Aged 30 to 40 Years Residing in Mumbai, India

The rising burden of non-communicable diseases (NCDs) in India necessitates more studies on nutritional intake and dietary behaviour. While data exists on low-income groups, rural populations and the population at large, limited information exists on that of urban, working professionals – a demographic that has the means and access to make informed choices, and yet, have a disproportionately high risk of NCDs. The aim of this study was to investigate nutrient intake in Indian professionals. A cross-sectional study was conducted on 214 working professionals (aged 30-40 years; 69 females and 145 males) in Mumbai. Habitual food intake was investigated by two 24-hour recalls and a semi-quantitative food frequency questionnaire. Nutrient adequacy ratios were calculated in comparison to Indian estimated average requirements. Compared to recommendations, a lower total energy and carbohydrate intake, while a higher protein and fat intake was observed among the participants. However, the intake of omega-3 fats, and overall fruit and vegetable intake was poor. A significantly higher energy from proteins and fats was observed among non-vegetarians, while vegetarians consumed more energy from carbohydrates. Insufficient intake of vitamins and minerals was prevalent with over 50% of study participants consuming &lt;50% of their required intake of Vitamin A, B12, folate, riboflavin, zinc and potassium. Intake of copper, pyridoxine and iron were also suboptimal. To conclude, the insufficient intake of micronutrient and macronutrient imbalances in the studied population, highlights the urgency of targeted nutritional interventions and education in urban, corporate populations in India, to mitigate the risk of NCDs.

Magnesium, selenium and zinc deficiency compromises antioxidant defense in women with obesity.

Studies have shown that deficiencies in magnesium, selenium, and zinc in individuals with obesity compromise the endogenous antioxidant defense system. This study aimed to evaluate the impact of mineral deficiency on enzymatic antioxidant defense in women with obesity. The study involved 63 women with obesity (BMI ≥ 35kg/m2) and 77 eutrophic women (BMI between 18.5 and 24.9kg/m2). Variables such as fasting glucose, glycated hemoglobin, fasting insulin, and serum lipids were analyzed. Insulin resistance was measured using the homeostasis assessment model (HOMA-IR) and beta cell function using the homeostasis assessment model (HOMA-β). Dietary intake of energy, macronutrients (including magnesium, zinc, and selenium), and plasma, erythrocyte, and urinary concentrations of these minerals were measured and analyzed. Serum cortisol, plasma leptin, plasma thiobarbituric acid reactive substances, and the activity of erythrocyte superoxide dismutase (SOD), erythrocyte glutathione peroxidase (GPX), and erythrocyte catalase were also analyzed. Women with obesity had reduced plasma and erythrocyte concentrations and greater urinary excretion of all minerals compared to normal weight women (p < 0.05). There was a positive association between erythrocyte concentrations of zinc and selenium and the activity of the GPX and SOD enzymes in erythrocytes in women with obesity (p < 0.05), in addition to a positive association between serum insulin and the enzyme GPX, which is dependent on dietary selenium (p < 0.05). Individuals with obesity are deficient in magnesium, selenium, and zinc, which appears to impair the antioxidant defense system and contribute to important metabolic disorders such as oxidative stress in these patients.

Plasma Antioxidant Capacity Is Related to Dietary Intake, Body Composition, and Stage of Reproductive Aging in Women.

women aging is a normal process of life; however, hormonal changes create an imbalance between prooxidants and antioxidants and could be measured as the antioxidant capability (AC) of an organism. to find the association between plasma AC levels, dietary intakes, and body composition in 18-64-year-old women living in the northeast of Mexico. A total of n = 514 women (18-64 years old) were grouped according to STRAW criteria as reproductive, menopausal transition, and postmenopausal. Anthropometrics, body mass index (BMI), weight-hip ratio (WHR), and weight-height ratio WHtR were determined, and percentage of body fat was analyzed by bioelectrical impedance. Dietary intake of macronutrients and vitamins A, E, and C were analyzed by a 3-day food recall. The AC status in plasma was analyzed by the ORACFL assay. Plasma AC levels were higher in postmenopausal women (815 µmol TE/L), and menopausal transition women (806 µmol TE/L) than in reproductive women (633 µmol TE/L). BMI was overweight (>25 kg/m2) in all three groups. WHtR and WHR are above the healthy limit of 0.5 and 0.8, respectively for both menopausal transition and postmenopausal women. In reproductive women, negative relationships were calculated between plasma AC and age (Rho = -0.250, p = 0.007), BMI (Rho = -0.473, p < 0.001), WHtR (Rho = -0.563, p < 0.001), WHR (Rho = -0.499, p < 0.001), and % body fat (Rho = -0.396, p < 0.001). A negative association was determined between plasma AC and WHtR in reproductive women (B = -2.718, p = 0.026). No association resulted for those in menopausal transition, and a positive association was obtained between plasma AC and protein (B = 0.001, p = 0.024) and vitamin E (B = 0.003, p = 0.013) intakes in postmenopausal women. the antioxidant capability (AC) in plasma was lower in reproductive women, and anthropometric parameters marking decreased physical fitness were associated with decreased AC.

Associations Between Dietary Macronutrient Intake and the Gut Microbiome in Adults Undergoing Bariatric Surgery for Obesity

Associations Between Dietary Macronutrient Intake and the Gut Microbiome in Adults Undergoing Bariatric Surgery for Obesity

A Cross-sectional Study on Dietary Diversity and Diet Cost in Type 2 Diabetic Population

Background: Globally, Type 2 Diabetes Mellitus is considered a major public health disease, influenced by nutrition and lifestyle patterns. The majority of people worldwide consume nutritionally poor, cereal-based diets. Therefore, assessing dietary diversity, macronutrient intake, and food costs is essential for better diabetes management. Objective: To compare the dietary diversity of the Type 2 diabetic population with estimated dietary costs and other relevant parameters. Methodology: A descriptive cross-sectional study was conducted among 150 adult Type 2 adult diabetic population. A pre-structured questionnaire was used to elicit information on dietary diversity using the Individual Dietary Diversity Score (IDDS), Food Variety Score (FVS) and diet cost estimation using semi-quantitative food frequency questionnaire. FVS was associated with nutrition status, Glycated Haemoglobin (HbA1c), macronutrient consumption, socio economic status and the average daily diet cost of the study population. Results: The majority of the population were overweight. 50% of the population had low dietary diversity and had uncontrolled diabetes with HbA1c of 7-8%. Dietary diversity influenced the nutritional status of diabetic people. There was no difference between socioeconomic and living status, HbA1c and FVS. Females had higher per day cost when compared to males. Mean per day increased with the increase in the macronutrient consumption. Conclusion: Diabetic patients often have low dietary diversity, which affects their nutritional status. Therefore, dietary diversity, along with diet cost, is integral to the effective nutritional care of the diabetic population.

Synergistic Effects of Nutrients on Musculoskeletal Health in Gerontology: Understanding the Combined Impact of Macronutrients and Micronutrients.

With the global aging population, addressing prevalent age-related conditions such as osteoporosis and sarcopenia is crucial. Traditional nutritional strategies focusing on single nutrients like calcium, vitamin D, or protein have limitations, prompting a nuanced exploration of the relationship between aging, nutrition, and musculoskeletal health. This cross-sectional study examines the complex interplay between dietary intake of macronutrients, common micronutrients, and water, as well as their association with musculoskeletal health in adults aged 50 to 80 years, using U.S. National Health and Nutrition Examination Survey data (NHANES). Employing multiple linear regression, restricted cubic splines, weighted quantile sum (WQS), and quantile-based g-computation (QGC) regression models, our initial analysis using the WQS model revealed that a one-quartile increase in mixed macronutrient intake was associated with a significant 0.009 unit increase in bone mineral density (BMD) and a 0.670 unit increase in grip strength, while a similar increase in mixed micronutrient intake showed a 0.007 unit increase in BMD and a 0.442 unit increase in grip strength. Our findings highlight the importance of a balanced dietary approach in promoting musculoskeletal health in the elderly, offering holistic strategies for overall well-being.

Mendelian randomization study supports relative carbohydrate intake as an independent risk factor for amyotrophic lateral sclerosis

ABSTRACT Objectives Observational studies suggested a potential correlation between dietary intake and amyotrophic lateral sclerosis (ALS), but conflicting findings exist and causality remains unclear. Here, we performed a Mendelian randomization (MR) analysis to evaluate the causal impact of relative intake of (i) carbohydrate, (ii) fat, and (iii) protein on ALS risk. Methods The genome-wide association summary statistics of three dietary macronutrient intake traits and ALS were obtained. Initially, forward and reverse univariable MR (UVMR) analysis were conducted using the inverse variance weighted (IVW) method as the primary approach, supplemented by MR-Egger, weighted median, and maximum likelihood. Subsequently, multivariable MR (MVMR) analysis was performed to assess the independent causal effects of each dietary. Additionally, diverse sensitivity tests were conducted to evaluate the reliability of the MR analyses. Results The forward UVMR analysis conducted by IVW indicated that relative carbohydrate intake significantly increased ALS risk. Furthermore, results from three other MR methods paralleled those from IVW. However, the other two dietary intake traits did not have a causative impact on ALS risk. The reverse UVMR analysis indicated that ALS did not causatively influence the three dietary intake traits. The MVMR analysis showed that after adjusting for the effects of the other two dietary intake traits, relative carbohydrate intake independently and significantly increased ALS risk. Sensitivity tests indicated no significant heterogeneity or horizontal pleiotropy. Discussion MR analysis supported relative carbohydrate independently increasing ALS risk. Nevertheless, further validation of this finding in future large cohorts is required. Abbreviations: ALS: amyotrophic lateral sclerosis; CI: confidence interval; GWAS: genome-wide association study; IV: instrumental variable; IVW: iverse variance weighted; MR: Mendelian randomization; MVMR: multivariable Mendelian randomization; OR: odds ratio; RCT: randomized controlled trial; SNPs: single-nucleotide polymorphisms; UVMR: univariable Mendelian randomization

Dominant Somatotype Development in Relation to Body Composition and Dietary Macronutrient Intake among High-Performance Athletes in Water, Cycling and Combat Sports

Relevant properties of the somatotype as important indicators can be associated with the body composition characteristics as well as both metabolic and bio-mechanical efficiency of athletes in the sport concerned. The primary aim of this single cross-sectional study was to determine the somatotype profiles in association with body composition and nutritional profiles among Lithuanian elite athletes (n = 189) involved in water, cycling and combat sports. The body composition along with the somatotype profiles and the nutritional status of athletes were evaluated using a battery of multiple frequency (5, 50, 250, 550, and 1000 kHz) bioelectrical impedance analysis (BIA) and a 3-day food record analysis. In terms of the prediction for athletes to be classified as endomorphs, mesomorphs or ectomorphs, the linear discriminant analysis was conducted to assess the grouping of samples. Both the multiple linear regression and multivariate logistic regression statistical analyses were performed to explore the associations between the independent and dependent variables. The central tendency values for the somatotype components of endomorphy, mesomorphy and ectomorphy in athletes playing water, cycling and combat sports were 4.3–4.9–3.4, 4.3–4.8–3.4 and 4.5–5.5–2.9, respectively. The central mesomorph somatotype with a trend towards endomorphy was dominant and varied according to a high muscle-to-fat ratio in elite athletes. Significant (p ≤ 0.001) positive associations between both endomorphy and mesomorphy values and higher body fat percentage as well as lower and upper limb muscle mass were identified. The lower levels of trunk muscle mass were related to athletes’ endomorphy and mesomorphy, too. Furthermore, in the athletes’ sample under analysis, high-level mesomorphs were prone to consume low-carbohydrate (adjusted odd ratio (AOR) 0.5, 95% confidence interval (CI) 0.2; 0.9) and high-protein diets (AOR 2.5, 95% CI 1.1; 5.5). Contrastingly, the elite athletes with a higher expression of endomorphy were on high-carbohydrate (AOR 5.4, 95% CI 1.1; 8.3) and high-fat diets (AOR 4.6, 95% CI 1.5; 7.1) along with insufficient protein diet (AOR 0.3, 95% CI 0.1; 0.9). Finally, whilst nutrition goals as a mediator can play a significant role in undergoing the maintenance of balance between the optimal body composition for athletic performance and the development of an ecto-mesomorphic somatotype, the elite athletes with higher levels of endomorphy value should be aware of lowering the body fat percentage coupled with dietary fat reduction and higher protein intakes. The findings obtained from the study may serve as an antecedent for a more targeted management of the elite athletes’ training process. Somatotyping as an additional assessment method can be successfully deployed in choosing correct coaching techniques, contributing to talent recognition processes or identifying reference morphometric parameters in elite athletes competing in water, cycling and combat sports.

Dietary Factors and Risk of Gout: A Two-Sample Mendelian Randomization Study.

Dietary intervention is the preferred approach for the prevention and clinical management of gout. Nevertheless, the existing evidence regarding the influence of specific foods on gout is insufficient. We used two-sample Mendelian randomization for genetic prediction to analyze the relationship between the intake of more than a dozen daily food items, such as pork, beef, cheese, and poultry, and dietary macronutrient intake (fat, protein, carbohydrates, and sugar) and the risk of developing gout and elevating the serum uric acid level. Inverse-variance weighted MR analyses were used as the main evaluation method, and the reliability of the results was tested by a sensitivity analysis. Cheese intake was associated with lower serum uric acid levels, and tea intake (OR = 0.523, [95%CI: 0.348~0.784], p = 0.002), coffee intake (OR = 0.449, [95%CI: 0.229~0.882], p = 0.020), and dried fruit intake (OR = 0.533, [95%CI: 0.286~0.992], p = 0.047) showed a preventive effect on the risk of gouty attacks. In contrast, non-oily fish intake (β = 1.08, [95%CI: 0.24~1.92], p = 0.012) and sugar intake (β = 0.34, [95%CI: 0.03~0.64], p = 0.030) were risk factors for elevated serum uric acid levels, and alcohol intake frequency (OR = 1.422, [95%CI: 1.079~1.873], p = 0.012) was a risk factors for gout predisposition. These results will significantly contribute to the formulation and refinement of nutritional strategies tailored to patients afflicted with gout.

Genetically predicted dietary macronutrient intakes and atrial fibrillation risk: a Mendelian randomization study

Background and aimPrevious observational investigations have indicated a potential association between relative dietary macronutrient intakes and atrial fibrillation and flutter (AF) risk. In this study, we employed Mendelian Randomization (MR) to evaluate the presence of causality and to elucidate the specific causal relationship.MethodsWe employed six, five, and three single nucleotide polymorphisms (SNPs) as instrumental variables for relative carbohydrate, protein, and fat intake, identified from a genome-wide association study that included 268,922 individuals of European descent. Furthermore, we acquired summary statistics for genome-wide association studies on AF from the FinnGen consortium, which involved 22,068 cases and 116,926 controls. To evaluate the causal estimates, we utilized the random effect inverse variance weighted method (IVW) and several other MR methods, including MR-Egger, weighted median, and MR-PRESSO, to confirm the robustness of our findings.ResultsOur analysis indicates a convincing causal relationship between genetically predicted relative carbohydrate and protein intake and reduced AF risk. Inverse variance weighted analysis results for carbohydrates (OR = 0.29; 95% CI (0.14, 0.59); P < 0.001) and protein (OR = 0.47; 95% CI (0.26, 0.85); P = 0.01) support this association. Our MR analysis did not identify a significant causal relationship between relative fat intake and AF risk.ConclusionOur study provides evidence supporting a causal relationship between higher relative protein and carbohydrate intake and a lower risk of atrial fibrillation (AF).

Abstract 754: Exploring the association among pain, gut microbiota, and dietary intake of macronutrients

Abstract Hematopoietic cell transplantation (HCT) is a potentially curative treatment for individuals diagnosed with hematological malignancies. As survival gains increase, symptom management is also being considered as an endpoint for treatment success yet there is paucity in the literature regarding biological correlates of symptoms. Pain is a major concern for patients undergoing HCT. Underlying inflammation has been suspected in the pathogenesis of symptoms including pain. Emerging evidence supports the gut microbiota (GM) as a potential pathway for the initiation of inflammation. Diet is a potentially modifiable factor demonstrating a significant impact on GM diversity and amenable to intervention. Understanding the interplay among pain, GM, and diet, particularly macronutrients, may inform the development of a targeted, dietary self-management intervention to mitigate pain symptoms in HCT patients. This investigation uses baseline data from a longitudinal descriptive study to explore associations among pain, GM composition (richness and evenness) using Shannon and Chao beta diversity indices, and macronutrients (protein, carbohydrates and fat), in 47 adults (&amp;gt; 18 years of age) receiving HCT for hematologic cancer. Pain was measured using the Brief Pain Inventory, GM was assessed using 16S ribosomal gene (V4 region) amplicons using Illumina MiSeq, and dietary information was collected using the Automated Self-Assessment 24-hour Dietary Assessment Tool (ASA-24). Data analysis was performed with the R ‘correlation’ package using Bayesian estimation of robust correlations and production of 95% Credibility Intervals (CI) and Bayes Factors (BF).The mean age of participants was 56.21 years + 14.67 years. Participants were diagnosed with myeloma (40%), lymphoma (29%) and leukemia (13%). The majority received autologous HCT (n=30). Macronutrient fat {r=-0.25, BF=1.72} had an inverse association with pain interference. Results provided at least anecdotal (BF &amp;gt; 1) support for a positive correlation between Healthy Eating Index (HEI) {r=0.21, BF=1.08}, total fat {r=0.32, BF=4.50}, carbohydrate {r=0.28, BF=2.47} and fat {r=0.32, BF=4.50}, and GM composition. Also, there was a positive association between the amount of protein {r=0.21, BF=1.03}, carbohydrate {r=0.27, BF=2.22}, and Chao Index. There was an inverse association between total HEI {r=-0.27, BF=1.87} and CRP levels. Taxonomic units {r=-0.41, BF=21.44}, Chao Index {r=-0.41, BF=20.28} and Shannon Index {r=-0.25, BF=1.47} had an inverse association with CRP level. This study associate pain, GM, and dietary macronutrients, suggesting potential GM targeted interventions. More research with larger sample size is needed to understand underlying dietary macronutrients' role in pain through GM diversity after HCT, aiming to ease symptoms and enhance survivorship. Citation Format: Elham Samami, Fan Yi, Michael Weaver, Angela Starkweather, Debra Lyon, Debra Lynch Kelly. Exploring the association among pain, gut microbiota, and dietary intake of macronutrients [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 754.

Low-carbohydrate-diet score, dietary macronutrient intake, and depression among adults in the United States

BackgroundThe aim was to ascertain whether low-carbohydrate-diet (LCD) score and dietary macronutrient intake are associated with depression. MethodsThis cross-sectional study included 23,204 United States adults from the National Health and Nutrition Examination Survey (NHANES) 2005–2018. Dietary macronutrient intake was evaluated by the average of two 24-h dietary recall interviews. LCD score was calculated by summing the 11 quantiles values of the percentages of energy derived from carbohydrate, protein, and fat. Major depression was defined as a nine-item Patient Health Questionnaire score of 10 or more. Logistic regression and restricted cubic spline models were used to explore the relationship between LCD score, dietary macronutrient intake, and depression. ResultsLCD score was significantly associated with the risk of depression after adjustment for covariates (odds ratio, 0.98; 95 % confidence interval, 0.97–0.99; p < 0.001). Restricted cubic splines showed that the pattern of this inverse association was nonlinear. Among macronutrients, carbohydrate and protein intake was nonlinearly associated with the risk of depression, whereas fat intake was not related to the risk of depression. A decreased risk of depression was observed when the carbohydrate intake was moderate (45.3 %–59.1 %). The pattern of the association between protein intake and the risk of depression was L-shaped. ConclusionsLCD score was inversely associated with the risk of depression in a nonlinear manner in a nationally representative sample of adults from the United States. Furthermore, moderate carbohydrate intake and high protein intake were correlated with a lower risk of depression.