Dicotyledonous Species Research Articles

In-Silico Study of Brassinosteroid Signaling Genes in Rice Provides Insight Into Mechanisms Which Regulate Their Expression.

Brassinosteroids (BRs) regulate a diverse spectrum of processes during plant growth and development and modulate plant physiology in response to environmental fluctuations and stress factors. Thus, the BR signaling regulators have the potential to be targeted for gene editing to optimize the architecture of plants and make them more resilient to environmental stress. Our understanding of the BR signaling mechanism in monocot crop species is limited compared to our knowledge of this process accumulated in the model dicot species - Arabidopsis thaliana. A deeper understanding of the BR signaling and response during plant growth and adaptation to continually changing environmental conditions will provide insight into mechanisms that govern the coordinated expression of the BR signaling genes in rice (Oryza sativa) which is a model for cereal crops. Therefore, in this study a comprehensive and detailed in silico analysis of promoter sequences of rice BR signaling genes was performed. Moreover, expression profiles of these genes during various developmental stages and reactions to several stress conditions were analyzed. Additionally, a model of interactions between the encoded proteins was also established. The obtained results revealed that promoters of the 39 BR signaling genes are involved in various regulatory mechanisms and interdependent processes that influence growth, development, and stress response in rice. Different transcription factor-binding sites and cis-regulatory elements in the gene promoters were identified which are involved in regulation of the genes’ expression during plant development and reactions to stress conditions. The in-silico analysis of BR signaling genes in O. sativa provides information about mechanisms which regulate the coordinated expression of these genes during rice development and in response to other phytohormones and environmental factors. Since rice is both an important crop and the model species for other cereals, this information may be important for understanding the regulatory mechanisms that modulate the BR signaling in monocot species. It can also provide new ways for the plant genetic engineering technology by providing novel potential targets, either cis-elements or transcriptional factors, to create elite genotypes with desirable traits.

Aldehyde dehydrogenase superfamily in sorghum: genome-wide identification, evolution, and transcript profiling during development stages and stress conditions

BackgroundAldehyde dehydrogenases (ALDHs) are a family of NAD(P)+ dependent enzymes that detoxify aldehydes by promoting their oxidation to respective carboxylic acids. The role of ALDH enzymes in various plant species has been extensively studied, revealing their critical role in salinity, drought, heat, and heavy metal stress tolerance. Despite their physiological significance, ALDH genes in Sorghum bicolor have yet to be studied thoroughly.ResultsIn this study, a total of 19 ALDH genes have been identified that have been grouped into ten families based on the criteria of the ALDH gene nomenclature committee. Segmental duplication assisted more in the enhancement of SbALDH gene family members than tandem duplication. All the identified SbALDH members made a cluster with monocot rice and maize in the phylogenetic tree rather than dicot species, suggesting the pre-eudicot-monocot separation of the ALDH superfamily members. The gene structure and protein domain were found to be mostly conserved in separate phylogenetic classes, indicating that each family played an important role in evolution. Expression analysis revealed that several SbALDHs were expressed in various tissues, developmental stages, and in response to abiotic stresses, indicating that they can play roles in plant growth, development, or stress adaptation. Interestingly, the majority of the SbALDH genes were found to be highly responsive to drought stress, and the SbALDH18B1 transcript showed maximum enhancement in all the stress conditions. The presence of cis-acting elements (mainly ABRE and MBS) in the promoter region of these genes might have a significant role in drought tolerance.ConclusionsOur findings add to the current understanding, evolutionary history, and contribution of SbALDHs in stress tolerance, and smooth the path of further functional validation of these genes.

Vegetation Composition, Forage Biomass and Soil Seed Bank of a Continuously Grazed Rangeland Site in Tropical Sub-Humid Environment, Tanzania

Most rangelands along the agro-pastoral villages of Tanzania are yearlong grazed and at various states of degradation. These rangelands contribute to over 60% of the meat and milk production in the country. An inventory was conducted to assess the status of grazing resources in a typical agro-pastoral village of Tanzania having communal rangelands. Systematic random sampling techniques were employed whereby line transects and quadrat frame were used following standard procedures to collect samples and undertake field measurements for both vegetation and soil parameters. The vegetation cover for desirable pasture species, undesirable pasture species and litter were 67.7%, 10.5% and 9.4%, respectively. The soil bare patches covered 12.3 % of the surveyed rangeland site. The most dominant grass species were Enteropogon macrostachyus, Bothriochloa insculpta and Heteropogon contortus. Forage dry matter (DM) yield was 806.8 kg DM/ha. Tree density was 1500 trees/ha and the total canopy cover was 63.49%. Combretum collinum was the most dominant tree species. Soil bulk density, pH, organic carbon, nitrogen, phosphorus and potassium were 1.4 g/cm3, 6.3%, 1.14%, 0.09%, 0.89 mg/kg and 0.33 g/kg, respectively. A total of 11 dicotyledonous species mainly forbs and 9 monocotyledonous species including two perennial grasses were revealed from the incubated soil samples. The findings of this study demonstrate that the communal grazing areas have low pasture productivity, poor soil seed-bank and high cover of woody plants mainly bushes. In order, to improve forage biomass at the study site and elsewhere with similar environments selective bush clearing and re-seeding should be considered.

BABY BOOM regulates early embryo and endosperm development.

The BABY BOOM (BBM) AINTEGUMENTA-LIKE (AIL) AP2/ERF domain transcription factor is a major regulator of plant cell totipotency, as it induces asexual embryo formation when ectopically expressed. Surprisingly, only limited information is available on the role of BBM during zygotic embryogenesis. Here we reexamined BBM expression and function in the model plant Arabidopsis thaliana (Arabidopsis) using reporter analysis and newly developed CRISPR mutants. BBM was expressed in the embryo from the zygote stage and also in the maternal (nucellus) and filial (endosperm) seed tissues. Analysis of CRISPR mutant alleles for BBM (bbm-cr) and the redundantly acting AIL gene PLETHORA2 (PLT2) (plt2-cr) uncovered individual roles for these genes in the timing of embryo progression. We also identified redundant roles for BBM and PLT2 in endosperm proliferation and cellularization and the maintenance of zygotic embryo development. Finally, we show that ectopic BBM expression in the egg cell of Arabidopsis and the dicot crops Brassica napus and Solanum lycopersicon is sufficient to bypass the fertilization requirement for embryo development. Together these results highlight roles for BBM and PLT2 in seed development and demonstrate the utility of BBM genes for engineering asexual embryo development in dicot species.

Multi-omics Analysis of Young Portulaca oleracea L. Plants' Responses to High NaCl Doses Reveals Insights into Pathways and Genes Responsive to Salinity Stress in this Halophyte Species.

The online version contains supplementary material available at 10.1007/s43657-022-00061-2.

The Impact of the Renovation of Grassland on the Development of Segetal Weeds in Organic Farming

The intensive use or discontinuation of the use of swards can compromise biodiversity, yields, and feed quality; thus, leading to the degradation of permanent grasslands. Various methods of renovation are employed to restore the usability of degraded swards. In the years 2013–2016, a monofactorial field experiment was carried out on the Experimental Farm in Grabów (province of Mazowieckie, Poland). The experiment involved swards being reseeded after ploughing (P) and after disking with a compact harrow (H), with a non-renovated sward as the control treatment. The plots under renovation were reseeded with a lucerne–grass mixture. Both seedbed preparation methods for the renewed sward, ploughing (P) or shallow disking to a depth of 5 cm (H), were found to be effective for increasing sward yields, restricting weed growth, and reducing the number of weeds in the sward. The ploughing-based renovation method (P) had a strongly restrictive effect on biodiversity, as expressed by the H’ function value vis-à-vis the harrow method (H) and the non-renewed control (NR). Dicotyledonous species accounted for 92.3% of the weed population in the studied treatments. Of these, Taraxacum officinale, Achillea milefolium, Capsella bursa-pastoris, and Plantago maior occurred in the highest numbers.

Transcriptomic and metabolomic landscape of quinoa during seed germination

BackgroundQuinoa (Chenopodium quinoa), a dicotyledonous species native to Andean region, is an emerging crop worldwide nowadays due to its high nutritional value and resistance to extreme abiotic stresses. Although it is well known that seed germination is an important and multiple physiological process, the network regulation of quinoa seed germination is largely unknown.ResultsHere, we performed transcriptomic study in five stages during transition from quinoa dry seed to seedling. Together with the GC–MS based metabolome analysis, we found that seed metabolism is reprogrammed with significant alteration of multiple phytohormones (especially abscisic acid) and other nutrients during the elongation of radicels. Cell-wall remodeling is another main active process happening in the early period of quinoa seed germination. Photosynthesis was fully activated at the final stage, promoting the biosynthesis of amino acids and protein to allow seedling growth. The multi-omics analysis revealed global changes in metabolic pathways and phenotype during quinoa seed germination.ConclusionThe transcriptomic and metabolomic landscape depicted here pave ways for further gene function elucidation and quinoa development in the future.

Microrelief of the leaf epidermis and stomatal polymorphism of Malus orientalis, Pyrus caucasica and Mespilus germanica in mountains and plains

The parameters of the stomatal apparatus were widely used for environmental monitoring. Thus, samples of stomata may include the size groups differing both in the time of formation and in the number of cuticular folds. Stomatal polymorphism was found in many dicotyledonous species, in particular, in subtribe Pyrinae (Rosaceae). Sample variability complicates the interpretation of the impact of environmental conditions. This work aimed to study the polymorphism of stomata and altitudinal effects in the model Pyrinae species. Scanning electron microscopy was used to study microrelief of leaf epidermis of Malus orientalis Uglitzk., Pyrus caucasica Fed. and Mespilus germanica L. growing in the mountains and on the plain (370–1750 m above sea level). Large single primary stomata and numerous small secondary stomata were observed. The stomata were surrounded by 2-5 bands of radial cuticular folds. Usually, the stomatal length linearly increased with an increase in the number of folds. The calculation of the mean length of stomata accounting for the stomatal polymorphism did not affect the conclusions, however, was more sensitive for assessing the altitude effect on stomata. The length of secondary stomata correlated inversely with their density. The sizes of epidermal cells on the abaxial and adaxial sides did not depend on the altitude. Pyrus caucasica and Mespilus germanica at an altitude of 600 and 800 m, respectively, had larger stomata comparing plants at higher altitudes. Altitudinal effects on stomatal length in Malus orientalis were less pronounced but had the same direction. In Mespilus germanica, the stomata in the steppe zone were also smaller. The decrease in stomata size is probably due to the sensitivity of plants to nonspecific stress, regardless of the altitude.

Influence of weather conditions and weediness on barley (Hordeum vulgare) yield

Barley belongs to the leading grain crops. This is due to its good adaptive abilities to various climatic conditions, as well as its high nutritional and feed value. However, this crop, when developed in the field, is forced to compete with weeds, which inevitably leads to a decrease in yield. The relationship between clogging, weather and climatic conditions and crop yields has been observed. However, for the geographical region of Siberia, there are few similar works exploring this relationship. Therefore, study on the influence of weather conditions and weeds on the formation of the yield of spring barley in the southeast of Western Siberia is a prerequisite for the development of the protection of this crop from weeds. This research included the study of the species composition of weeds and hydrothermal characteristics and their influence on the formation of the yield of spring barley. The studies were carried out in the steppe zone of the Kuznetsk Basin and the Mariinsky forest-steppe of the West Siberian Plain of the Kemerovo Region (Russia) from 2014 to 2018. To characterize the dynamics of yield, sown areas and gross harvests of spring barley, data from statistical bulletins of the territorial body of the Federal State Statistics Service for the Kemerovo region were used. An increase in the yield of barley was observed. In 2018, compared to 2014, the difference was 0.19 t/ha and revealed a significant reduction in acreage and, due to this, a decrease in the gross yield of barley was noted. Thirtyfour species of weeds belonging to 16 families were recorded on the plot. The biological groups of weeds are represented by six types: ephemera, early spring, late spring, annual wintering, rhizome perennials, and root suckers. The yield of spring barley is greatly reduced by dicotyledonous biennial species of weeds from the Cabbage and Bluegrass families.

Carbonate-Induced Chemical Reductants Are Responsible for Iron Acquisition in Strategy I Wild Herbaceous Plants Native to Calcareous Grasslands.

Significant progress has been made in understanding Strategy I iron (Fe) acquisition using crop/model plants under controlled conditions in laboratories. However, plant species native to calcareous soils may have evolved unique strategies for adaptation to high carbonate/pH-induced Fe deficiency. Until now, little information is available on the Fe acquisition mechanisms in these plants. Here, we explored the Fe acquisition mechanisms in wild dicot species native to calcareous grasslands, by monitoring the Fe nutrition-related rhizosphere processes in field and greenhouse conditions. Most of these wild species displayed comparable shoot Fe concentration to those of crops, and some dicots actually accumulated very high shoot Fe. However, these species did not exhibit ferric reductase oxidase (FRO)-dependent Strategy I responses to Fe deficiency, including visual rhizosphere acidification and increased Fe3+ reduction. In contrast, chemical reductants exuded by roots of dicots were responsible for Fe3+ reduction in these wild plants. These features were not observed in the FRO-dependent Strategy I crop plant cucumber. Neither leaf chlorophyll nor shoot/root Fe was depressed by 10% CaCO3 application in all the examined wild species. Furthermore, their root exudation was significantly activated by CaCO3, leading to an increased Fe3+ reduction. We show that chemical reductant-mediated Fe3+ reduction occurs preferentially in these wild dicots and that these mechanisms are not sensitive to high soil carbonate/pH. Our findings support that Fe acquisition in Strategy I wild plants native to calcareous soils is substantially different from the enzyme-dependent system of Strategy I plants.

Live-cell imaging elaborating epidermal invasion and vascular proliferation/colonization strategy of Verticillium dahliae in host plants.

The soilborne ascomycete fungus Verticillium dahliae causes destructive vascular wilt disease in hundreds of dicotyledonous plant species. However, our understanding of the early invasion from the epidermis to the vasculature and the prompt proliferation and colonization in the xylem tissues remains poor. To elaborate the detailed infection strategy of V. dahliae in host plants, we traced the whole infection process of V. dahliae by live‐cell imaging combined with high‐resolution scanning electron microscopy. The 4D image series demonstrated that the apex of invading hyphae becomes tapered and directly invades the intercellular space of root epidermal cells at the initial infection. Following successful epidermal invasion, the invading hyphae extend in the intercellular space of the root cortex toward the vascular tissues. Importantly, the high‐resolution microscopic and live‐cell images demonstrated (a) that conidia are formed via budding at the apex of the hyphae in the xylem vessels to promote systemic propagation vertically, and (b) that the hyphae freely cross adjacent xylem vessels through the intertracheary pits to achieve horizontal colonization. Our findings provide a solid cellular basis for future studies on both intracellular invasion and vascular colonization/proliferation during V. dahliae infection and pathogenesis in host plants.

Alterations in phytotoxicity and allelochemistry in response to intraspecific variation in Parthenium hysterophorus

Allelopathy plays a crucial role in providing competitive advantage to several alien invasive species, and assists in their establishment beyond native boundaries. Role of allelopathy in the invasion success of the alien weed, Parthenium hysterophorus is well established; however, the ecological and evolutionary factors that could affect its allelopathic interactions are relatively unexplored. In our earlier findings, we suggested the presence of two morphotypes (PA and PB) in the population of P. hysterophorus, with variable morphology, physiology, and level of ecological impacts. Here, we hypothesize that phenotypically distinct morphotypes of P. hysterophorus may vary in their phytotoxicity and allelochemistry, thereby producing differential ecological impact. To test this hypothesis, effects of rhizospheric soil (RS) and plant amended soils (PAS) of the two morphotypes of P. hysterophorus (PA and PB) were studied on selected dicot (Bidens pilosa and Senna occidentalis) and monocot species (Phalaris minor and Avena fatua). Also, the composition of allelochemicals in PA and PB was assessed using Liquid Chromatography and Mass Spectrometry (LC-MS). The study revealed that the phytotoxic effect of PB was greater than PA in all the parameters measured for dicot species and in most of the parameters studied in monocot species. A generalized dose-dependent response was observed in the test species (PAS0 < PAS10 < PAS20 < PAS40) and the effect of RS was comparable to PAS20 and PAS40. A greater number of allelochemicals were reported from PB, which is in accordance with the growth studies. The study concludes that intraspecific variations account for differential phytotoxicity and allelochemistry in P. hysterophorus.

Genetic Control of Seed Phytate Accumulation and the Development of Low-Phytate Crops: A Review and Perspective

Breeding low phytic acid (lpa) crops is a strategy that has potential to both improve the nutritional quality of food and feed and contribute to the sustainability of agriculture. Here, we review the lipid-independent and -dependent pathways of phytate synthesis and their regulatory mechanisms in plants. We compare the genetic variation of the phytate concentration and distribution in seeds between dicot and monocot species as well as the associated temporal and spatial expression patterns of the genes involved in phytate synthesis and transport. Quantitative trait loci or significant single nucleotide polymorphisms for the seed phytate concentration have been identified in different plant species by linkage and association mapping, and some genes have been cloned from lpa mutants. We summarize the effects of various lpa mutations on important agronomic traits in crop plants and propose SULTR3;3 and SULTR3;4 as optimal target genes for lpa crop breeding.

Targeted generation of Null Mutants in ZmGDIα confers resistance against maize rough dwarf disease without agronomic penalty.

Targeted generation of Null Mutants in ZmGDIα confers resistance against maize rough dwarf disease without agronomic penalty.

Ethnobotanical uses of plants and constrains in Pathra and its adjoining areas, Paschim Medinipur District, West Bengals

A survey was conducted in to collect the information about the plant diversity and uses of these plants by the local people in different purposes Pathra and its adjoining village areas in Paschim Medinipur district, W.B. This area is beside river Kangsabati, popular due to presence of some archaeological structures and natural beauty. The ethnobotanical study revealed that different plant species which is used by the villagers of Pathra, depends on these local plants, which have various economical aspects. Different parts of these plants help in the production of timber, medicine, jewellery. Fruit yielding plants and ornamental plants are also there. Different species of fungi, pteridophytes, monocots and dicots are there which created a rich diversity. But new generation tends to switch off towards chemical substitutes of the ethno-products. Now-a-days popularity of the area draws group of people to come here to celebrate different festivals like Holi and picnic. These situations lead to a negative impact on natural biodiversity of this area and its conservation, by loss of endangered plants, medicinal plants including soil erosion, soil toxicity as plastics and other nondegradable materials used by the tourists has deposited on the soil. In this paper we are trying to spread the information about the values of these local plants and also trying to aware the people to save the diversity of this area.

Salt tolerance in relation to elemental concentrations in leaf cell vacuoles and chloroplasts of a C4 monocotyledonous halophyte.

Halophytes accumulate and sequester high concentrations of salt in vacuoles while maintaining lower levels of salt in the cytoplasm. The current data on cellular and subcellular partitioning of salt in halophytes are, however, limited to only a few dicotyledonous C3 species. Using cryo‐scanning electron microscopy X‐ray microanalysis, we assessed the concentrations of Na, Cl, K, Ca, Mg, P and S in various cell types within the leaf‐blades of a monocotyledonous C4 halophyte, Rhodes grass (Chloris gayana). We also linked, for the first time, elemental concentrations in chloroplasts of mesophyll and bundle sheath cells to their ultrastructure and photosynthetic performance of plants grown in nonsaline and saline (200 mM NaCl) conditions. Na and Cl accumulated to the highest levels in xylem parenchyma and epidermal cells, but were maintained at lower concentrations in photosynthetically active mesophyll and bundle sheath cells. Concentrations of Na and Cl in chloroplasts of mesophyll and bundle sheath cells were lower than in their respective vacuoles. No ultrastructural changes were observed in either mesophyll or bundle sheath chloroplasts, and photosynthetic activity was maintained in saline conditions. Salinity tolerance in Rhodes grass is related to specific cellular Na and Cl distributions in leaf tissues, and the ability to regulate Na and Cl concentrations in chloroplasts.

Development of an efficient marker-free soybean transformation method using the novel bacterium Ochrobactrum haywardense H1.

SummaryWe have discovered a novel bacterium, Ochrobactrum haywardense H1 (Oh H1), which is capable of efficient plant transformation. Ochrobactrum is a new host for Agrobacterium‐derived vir and T‐DNA‐mediated transformation. Oh H1 is a unique, non‐phytopathogenic species, categorized as a BSL‐1 organism. We engineered Oh H1 with repurposed Agrobacterium virulence machinery and demonstrated Oh H1 can transform numerous dicot species and at least one monocot, sorghum. We generated a cysteine auxotrophic Oh H1‐8 strain containing a binary vector system. Oh H1‐8 produced transgenic soybean plants with an efficiency 1.6 times that of Agrobacterium strain AGL1 and 2.9 times that of LBA4404Thy‐. Oh H1‐8 successfully transformed several elite Corteva soybean varieties with T0 transformation frequency up to 35%. In addition to higher transformation efficiencies, Oh H1‐8 generated high‐quality, transgenic events with single‐copy, plasmid backbone‐free insertion at frequencies higher than AGL1. The SpcN selectable marker gene is excised using a heat shock‐inducible excision system resulting in marker‐free transgenic events. Approximately, 24.5% of the regenerated plants contained only a single copy of the transgene and contained no vector backbone. There were no statistically significant differences in yield comparing T3 null‐segregant lines to wild‐type controls. We have demonstrated that Oh H1‐8, combined with spectinomycin selection, is an efficient, rapid, marker‐free and yield‐neutral transformation system for elite soybean.

Genome-Wide Analysis of Serine Hydroxymethyltransferase Genes in Triticeae Species Reveals That TaSHMT3A-1 Regulates Fusarium Head Blight Resistance in Wheat.

Serine hydroxymethyltransferase (SHMT) plays a pivotal role in cellular one-carbon, photorespiration pathways and it influences the resistance to biotic and abiotic stresses. However, the function of SHMT proteins in wheat remains largely unexplored. In the present study, SHMT genes in five Triticeae species, Oryza sativa, and four dicotyledon species were identified based on whole genome information. The origin history of the target gene was traced by micro-collinearity analysis. Gene expression patterns of TaSHMTs in different tissues, various biotic stresses, exogenous hormones, and two biotic stresses were determined by Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The function of the selected TaSHMT3A-1 was studied by barley stripe mosaic virus-induced gene silencing in common wheat Bainong207. A total of 64 SHMT members were identified and further classified into two main classes based on the structure of SHMT proteins. The gene structure and motif composition analyses revealed that SHMTs kept relatively conserved within the same subclasses. Interestingly, there was a gene, TdSHMT7B-1, on chromosome 7B of Triticum dicoccoides, but there was no SHMT gene on chromosome 7 of other analyzed Triticeae species; TdSHMT7B-1 had fewer exons and conserved motifs than the genes in the same subclass, suggesting that the gene of TdSHMT7B-1 has a notable evolutionary progress. The micro-collinearity relationship showed that no homologs of TaSHMT3A-1 and its two neighboring genes were found in the collinearity region of Triticum urartu, and there were 27 genes inserted into the collinearity region of T. urartu. Furthermore, qRT-PCR results showed that TaSHMT3A-1 was responsive to abiotic stresses (NaCl and cold), abscisic acid, methyl jasmonate, and hydrogen peroxide. Significantly, upon Fusarium graminearum infection, the expression of TaSHMT3A-1 was highly upregulated in resistant cultivar Sumai3. More importantly, silencing of TaSHMT3A-1 compromises Fusarium head blight resistance in common wheat Bainong207. Our new findings suggest that the TaSHMT3A-1 gene in wheat plays an important role in resistance to Fusarium head blight. This provides a valuable reference for further study on the function of this gene family.

Effectiveness of Herbicides in Winter Wheat Crops

To obtain high yields of winter wheat, it is important to control the contamination of crops with weeds. It is known that yield losses can be 30% or more due to the presence of segetal vegetation in wheat crops. With intensive technology of growing winter wheat, the chemical method of protection against weeds is an extremely important element. The paper highlights data on the species composition and class of contamination of winter wheat agrocenosis. The effectiveness of herbicides and their tank mixtures in winter wheat crops to reduce the presence of the weed component, increase grain yield and quality in the conditions of Luginsky District of Zhytomyr region on sandy loam soils during 2019-2021 was studied. It is established that the structure of the weed component of agrophytocenosis of winter wheat in farm conditions is dominated by dicotyledonous weed species, namely: field mustard (Sinapis arvensis L.), wild radish (Raphanus raphanistrum L.), wild pansy (Viola tricolor L.), shovelweed (Capsella bursa-pastoris L.), toadpipe (Equisetum arvense L.), and blue bottle flower (Centaurea cyanus L.), the share of which is 64.8%. Graminaceous species of weeds are represented by silky bent grass (Apera spica-venti L.), couch-grass (Elytrigia repens L.), rye brome grass (Bromus secalinus L.). The technical efficiency of the studied herbicides with the separate and joint application was in the range of 60.8-94.6%. The highest indicator of technical efficiency of herbicides in winter wheat crops, which is 94.6%, was obtained in an experiment using a tank mixture of Granstar Pro 75 + Apiros 75 preparations, with norms of 0.025+0.013 kg/ha, as well as an increase in wheat grain yield by 2.0 t/ha compared to the contaminated control. Spraying winter wheat crops with herbicides during the growing season allows improving the quality indicators of the grain yield structure. In particular, the content of protein and fibrin increases by 1.1 times; the moisture content of grain decreases by 1.0-1.1 times, and the content of dirt impurity decreases by 2.8-6.7 times, compared to the control version

Iron biofortification through genetic modification in rice, wheat, and cassava and its potential contribution to nutritional security

Abstract Micronutrient malnutrition is one of the major concerns noted in the United Nations Sustainable Development Goals. In developing countries, children under the age of five and pregnant women are at the highest risk of adverse events from micronutrient malnutrition. Staple food crops that are common in developing countries are rich in carbohydrates but low in micronutrients, especially iron. Genetic biofortification of staple food crops in farmer-preferred cultivars is a promising approach to deliver nutritious food, enhanced in iron concentration, to consumers who are at risk of malnutrition. In order to achieve biofortification, it is critical to understand the processes of iron uptake, regulation, transport dynamics, and whole-plant iron storage and how to manipulate them in individual crops. In this review, case studies of staple food crops including rice, wheat, and cassava are used to introduce iron biofortification strategies in both monocotyledonous and dicotyledonous species, along with a discussion of consumer-based considerations for the deployment of biofortified crops also presented.