Developmental Phenomena Research Articles

Development of Interactional Competence in a Virtual Exchange Setting: Focus on Diversification of Participatory Actions

Virtual exchange (VE) projects create environments providing VE participants with L2 development opportunities. This study examines the affordances of VEs with a particular emphasis on how participants find opportunities for developing their interactional competences in video-mediated environments. The data comes from a VE Project organized among three universities based in Germany, Türkiye and Sweden. During the project timeline, the participants worked in teams through weekly meetings. The data comprises screen-recordings of video-mediated team exchange meetings and written final reflection papers. The screen-recorded data was analyzed via longitudinal Conversation Analysis (CA), while the reflection papers were examined to identify potential developmental phenomena. That is, one student claiming to have improved her interactional competence in the final reflection paper was identified as the focal participant. Retrospective tracking of this student’s entire video-mediated interactional history with her team-members during the VE project (8 hours across 3 months) revealed a diversification of her participatory actions over time manifested through changes in her involvement (i.e., becoming increasingly active) in the team interaction. Initially, she participated minimally with embodied or short responses solicited by the others, and remained mostly silent. However, in subsequent team exchanges, she not only displayed unsolicited contributions by elaborating and topic-shifting but also took an active role in the VE team meetings by opening tasks/conversations, sharing her stance towards various task-related proposals, and using mitigated disagreement practices for problem-solving about the collaborative team-product. Using longitudinal CA, this study brings evidence for a VE participant’s interactional competence development in terms of participatory actions during turn-entry moments in video-mediated interaction, through which she became a more active participant in the teamwork. By providing instances to document this change in her participatory actions, the study discusses to what extent VE settings can contribute to a participant’s L2 interactional competence.

Why Should Developmental Scientists Engage in Research on Adolescent Suicide? The Case for Developmentally Informed Suicidology

Adolescent suicide is a critical and prevalent issue yet is rarely studied by developmental psychologists. We assert that developmentalists’ lack of engagement has stymied progress on adolescent suicide research and practice, and we demonstrate how developmental perspectives can contribute to the next decade of research and practice in suicidology. Specifically, we (a) review theories in suicidology (that are largely a-developmental); (b) discuss how developmental phenomena may explain findings regarding the epidemiology of adolescent suicide; (c) review the evidence on the etiology of adolescent suicide, where efforts to identify risk factors have not led to strong predictions, highlighting underutilized approaches from the developmental risk and resilience literature; and (d) review strategies and developmental gaps across the suicide prevention continuum from primary prevention to postvention, recommending an adolescent developmental perspective on the development and study of school- and community-based suicide prevention and postvention. In doing so, we propose a new subfield of Developmentally Informed Suicidology.

Evidence for Infant-directed Speech Preference Is Consistent Across Large-scale, Multi-site Replication and Meta-analysis.

There is substantial evidence that infants prefer infant-directed speech (IDS) to adult-directed speech (ADS). The strongest evidence for this claim has come from two large-scale investigations: i) a community-augmented meta-analysis of published behavioral studies and ii) a large-scale multi-lab replication study. In this paper, we aim to improve our understanding of the IDS preference and its boundary conditions by combining and comparing these two data sources across key population and design characteristics of the underlying studies. Our analyses reveal that both the meta-analysis and multi-lab replication show moderate effect sizes (d ≈ 0.35 for each estimate) and that both of these effects persist when relevant study-level moderators are added to the models (i.e., experimental methods, infant ages, and native languages). However, while the overall effect size estimates were similar, the two sources diverged in the effects of key moderators: both infant age and experimental method predicted IDS preference in the multi-lab replication study, but showed no effect in the meta-analysis. These results demonstrate that the IDS preference generalizes across a variety of experimental conditions and sampling characteristics, while simultaneously identifying key differences in the empirical picture offered by each source individually and pinpointing areas where substantial uncertainty remains about the influence of theoretically central moderators on IDS preference. Overall, our results show how meta-analyses and multi-lab replications can be used in tandem to understand the robustness and generalizability of developmental phenomena.

Polaryzacja: polemika z artykułem „Fanatyzm jako problem wychowawczy”

The criticism of the article “Fanaticism as an Educational Problem” focuses on the selective treatment of fanaticism symptoms, ignoring the role of political polarization and age in analysing this phenomenon. Gałkowski and Gałkowski’s article (2023) presents a valuable proposal for a minimal educational program, but it requires considering a broader social, cultural, and individual context in diagnosing fanaticism and applying effective tools to counteract this phenomenon. The starting point of the debate is the observation that fanaticism remains a negatively perceived label that excludes individuals or social groups, hence the need for clarification on how cultural diversity and intercultural communication can contribute to it. Fanaticism is associated with an existential crisis, but there are also specific developmental phenomena in children that can be mistaken for fanaticism. Literary works can help in understanding this phenomenon. The proposed examples indicate that fanaticism is primarily a group phenomenon characterized by action rather than solely an attitude to the truth. In preventing and dismantling fanaticism, it is important to consider emotions related to uncertainty and develop skills to cope with it. Finding effective alternatives to psychoactive substances in emotional regulation and violence prevention poses a challenge. Fanaticism can be present in the inertia of customs and can be recognized by sensitive young people in society’s reactions to demands for social change. The situation of individuals expelled at the Polish-Belarusian border highlights the difference between uncompromising commitment and fanaticism. Human rights should be respected without exceptions, and the willingness to accept external judgment distinguishes activists from fanatics. Building relationships free from fanaticism in communities, especially in the case of refugees, requires considering the atmosphere and pedagogy of the place, which play a crucial role in combating fanaticism.

Prematuritas Berdampak Terjadinya Hiperbilirubinemia Pada Neonatus Di Perinatologi RS Dr. Hafiz Cianjur

Prematurity is the birth or delivery of a baby that occurs between 20 weeks gestation to less than 37 weeks. About 25-50% of newborns suffer from jaundice in the first week. Hyperbilirubinemia in infants less months has a higher incidence rate. These developmental and clinical phenomena contribute to a greater degree and duration of neonatal hyperbilirubinemia in premature infants. Objective: to analyze the relationship of prematurity with hyperbilirubinemia in neonates at Perinatology Dr. Hafiz Cianjur Hospital. Method: The design used is quantitative observational with a cross-sectional approach. Samples were taken as many as 60 neonates using total sampling techniques. The research data was taken using medical record sheets in October – December 2023. Results: After analyzing the data was tabulated using a chi square test with a meaning level of 0.05. The results showed Prematurity (51.7%) as many as 31 neonates gestation period 31-36 weeks and Hyperbilirubinemia (86.7%) as many as 52 neonates in the severe category. The results of statistical testing obtained a significancy value of 0.003. Based on the results of p value < 0.05. Conclusion: it can be concluded that there is a relationship between prematurity and hyperbilirubinemia in neonates in Hospital Perinatology.

A unified approach to demographic data collection for research with young children across diverse cultures.

Culture is a key determinant of children's development both in its own right and as a measure of generalizability of developmental phenomena. Studying the role of culture in development requires information about participants' demographic backgrounds. However, both reporting and treatment of demographic data are limited and inconsistent in child development research. A barrier to reporting demographic data in a consistent fashion is that no standardized tool currently exists to collect these data. Variation in cultural expectations, family structures, and life circumstances across communities make the creation of a unifying instrument challenging. Here, we present a framework to standardize demographic reporting for early child development (birth to 3 years of age), focusing on six core sociodemographic construct categories: biological information, gestational status, health status, community of descent, caregiving environment, and socioeconomic status. For each category, we discuss potential constructs and measurement items and provide guidance for their use and adaptation to diverse contexts. These items are stored in an open repository of context-adapted questionnaires that provide a consistent approach to obtaining and reporting demographic information so that these data can be archived and shared in a more standardized format. (PsycInfo Database Record (c) 2024 APA, all rights reserved).

Growth hormone directly stimulates GATA2 expression

ObjectiveGATA2 is a key transcription factor involved in the differentiation and determination of thyrotrophs and gonadotrophs in pituitary and hematopoietic development. However, studies on the upstream ligands of the GATA2 signal transduction pathway have been limited. To identify upstream ligands, we examined growth hormone (GH) as a plausible stimulator. DesignWe evaluated GH-induced GATA2 expression in murine TtT/GF thyrotrophic pituitary tumor cells and its direct impact on the GHR/JAK/STAT5 pathway using a combination of a reporter assay, real-time quantitative polymerase chain reaction, and western blotting. ResultsGATA2 expression increased with activated STAT5B in a dose-dependent manner and was inhibited by a STAT5 specific inhibitor. Moreover, we found functional STAT5B binding site consensus sequences at −359 bp in the GATA2 promoter region. ConclusionThese findings suggest that GH directly stimulates GATA2 via the GHR/JAK/STAT pathway and participates in various developmental phenomena mediated by GATA2.

Exploring Co-adaptation in an Ecosystem of Dyadic Interaction

In the field of applied linguistics, Diane Larsen-Freeman is widely recognized, among her numerous contributions, as the originator of Complex Dynamic Systems Theory (CDST). Over the years since her seminal publication (Larsen-Freeman, 1997), CDST has evolved into a meta-theory that guides the field’s thinking on a variety of developmental phenomena, not least the study of second language acquisition. As its theoretical potency rises, so does the need to achieve a tangible understanding of CDST’s core constructs. In this paper, we empirically examine one such construct, co-adaptation, in the context of asynchronous dyadic interaction. A data corpus of 39 emails sent between two college students in China and the United States over the course of seven weeks is analyzed using NVivo 12. Co-adaptation is observed at the pragmatic, discourse, and linguistic levels. True to the spirit of CDST, we describe, and discuss the nuances of, our findings. Considering the novelty of our approach to data analysis, we end by reflecting on the limitations specific to our study, as well as worthwhile directions for future pursuits.

Formalizing developmental phenomena as continuous-time systems: Relations between mathematics and language development.

We demonstrate how developmental theories may be instantiated as statistical models, using hierarchical continuous-time dynamic systems. This approach offers a flexible specification and an often more direct link between theory and model parameters than common modeling frameworks. We address developmental theories of the relation between the academic competencies of mathematics and language, using data from the online learning system Mindsteps. We use ability estimates from 160,164 observation occasions, across N = 4623 3rd to 9th grade students and five ability domains. Model development is step-by-step from simple to complex, with ramifications for theory and modeling discussed at each step.

Early triadic interactions in the first year of life: a systematic review on object-mediated shared encounters.

Infants' early interactions with adults and everyday objects are key to socio-communicative development, but their emergence and development are still under debate. Aiming at describing the diversity of theoretical and methodological approaches on triadicity during the first year of life, we conducted a systematic and qualitative review of recent literature. Following PRISMA 2020 guidelines, we explored the scientific production of recent decades on triadic interactions up to 12 months of age. We initially screened 1943 items from which we obtained a final sample of 51 publications. Studies are usually conducted in laboratory settings, while ecological research is becoming increasingly common, especially in home settings. According to a thematic analysis of the data, we discussed the different perspectives on the origin and conceptualization of triadic interactions, and how they contribute to structuring and facilitating other developmental phenomena, such as the children's communicative gestures and uses of objects. Prior to the origin of intentional communication, adults facilitate early forms of triadicity based on fostering opportunities for infants' communication and engagement with both adults and materiality. However, there is a need for further research that explore the potential of early triadic interactions for parenting and early childhood education practises.

Natural lignin modulators improve lignocellulose saccharification of field-grown sugarcane, soybean, and brachiaria

Saccharification of lignocellulosic biomass might contribute to the bioethanol production, positively impacting energy security and sustainability. Lignin reduces polysaccharide saccharification and needs to be depolymerized and separated to improve fermentable sugar yield. Here, we report a substantial increase in lignocellulose saccharification after treating plants with the natural compounds: 3,4-(methylenedioxy)cinnamic acid (MDCA), a competitive inhibitor of 4-coenzyme A ligase (4CL); piperonylic acid (PIP), a quasi-irreversible inhibitor of cinnamyl 4-hydroxylase (C4H); and daidzin (DZN) a competitive inhibitor of coniferaldehyde dehydrogenase (CALDH). A single treatment with these inhibitors on different young developmental stages significantly increased lignocellulosic saccharification of crops at maturity stage. Field trial experiments with sugarcane (Saccharum spp), soybean (Glycine max), and brachiaria (Urochloa decumbens), showed that 12-month-old sugarcane bagasse increased saccharification up to 120%, 90-days-old soybean residues increased saccharification up to 36%, and 40-days-old brachiaria straw increased saccharification up to 21%. Treatments did not affect biomass productivity, lignin content or its monomeric composition. In contrast, anatomic assessment by UV microscopy revealed that MDCA affected tissue formation, increasing the lignification in fibers and vessels, but not in parenchyma. The tissue-specific and developmental persistent phenomena allowed a substantial increase in saccharification, while preserving the mechanical strength necessary for plant growth and development, suggesting that natural compounds are opportune for bioenergy applications.

Multifunctional chemical inhibitors of the florigen activationcomplex discovered by structure-based high-throughput screening.

Structure-based high-throughput screening of chemical compounds that target protein-protein interactions (PPIs) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At present, there are no examples of using high-throughput screening to identify chemicals that target plant transcriptional complexes, some of which are responsible for regulating multiple physiological functions. Florigen, a protein encoded by FLOWERING LOCUS T (FT), was initially identified as a molecule that promotes flowering and has since been shown to regulate flowering and other developmental phenomena such as tuber formation in potato (Solanum tuberosum). FT functions as a component of the florigen activation complex (FAC) with a 14-3-3 scaffold protein and FD, a bZIP transcription factor that activates downstream gene expression. Although 14-3-3 is an important component of FAC, little is known about the function of the 14-3-3 protein itself. Here, we report the results of a high-throughput invitro fluorescence resonance energy transfer (FRET) screening of chemical libraries that enabled us to identify small molecules capable of inhibiting FAC formation. These molecules abrogate the invitro interaction between the 14-3-3 protein and the OsFD1 peptide, a rice (Oryza sativa) FD, by directly binding to the 14-3-3 protein. Treatment with S4, a specific hit molecule, strongly inhibited FAC activity and flowering in duckweed, tuber formation in potato, and branching in rice in a dose-dependent manner. Our results demonstrate that the high-throughput screening approach based on the three-dimensional structure of PPIs is suitable in plants. In this study, we have proposed good candidate compounds for future modification to obtain inhibitors of florigen-dependent processes through inhibition of FAC formation.

Object labeling activates young children’s scale errors at an early stage of verb vocabulary growth

Scale errors are intriguing developmental phenomena in which young children attempt to perform impossible object-specific actions toward miniature-sized objects. Of several related cognitive abilities, lexical development during toddlerhood enhances scale error production by making objects’ semantic representations dominant over perceptual information. To directly address the effect of activated semantic representations on scale errors, we examined whether and when object labeling affected scale errors. Toddlers aged 18 to 30 months (N = 72) performed a body-based scale error task twice: in one session with specific object labels provided (e.g., “chair”) and in the other session with general pronouns provided (e.g., “this”). Using different developmental indices, including chronological age and productive vocabulary size of nouns, verbs, and adjectives, the enhancement effect of object labeling was detected only for children whose verb vocabulary size was classified into the medium group (3–26 words). Moreover, verb vocabulary size was determined to be the best predictor of scale error production among the candidate developmental indices. We also found that toddlers produced more scale errors in the first session that they performed the task compared with the second session. In addition to revealing that careful control of relevant factors (e.g., developmental indices, labeling, task repetition) is required for scale error research, this study sheds light on the relevance of verb vocabulary on scale errors.

A Simple Microfluidic Chip for Long-Term Growth and Imaging of <em>Caenorhabditis elegans</em>

Caenorhabditis elegans (C. elegans) have proved to be a valuable model system for studying developmental and cell biological processes. Understanding these biological processes often requires long-term and repeated imaging of the same animal. Long recovery times associated with conventional immobilization methods done on agar pads have detrimental effects on animal health making it inappropriate to repeatedly image the same animal over long periods of time. This paper describes a microfluidic chip design, fabrication method, on-chip C. elegans culturing protocol, and three examples of long-term imaging to study developmental processes in individual animals. The chip, fabricated with polydimethylsiloxane and bonded on a cover glass, immobilizes animals on a glass substrate using an elastomeric membrane that is deflected using nitrogen gas. Complete immobilization of C. elegans enables robust time-lapse imaging of cellular and sub-cellular events in an anesthetic-free manner. A channel geometry with a large cross-section allows the animal to move freely within two partially sealed isolation membranes permitting growth in the channel with a continuous food supply. Using this simple chip, imaging of developmental phenomena such as neuronal process growth, vulval development, and dendritic arborization in the PVD sensory neurons, as the animal grows inside the channel, can be performed. The long-term growth and imaging chip operates with a single pressure line, no external valves, inexpensive fluidic consumables, and utilizes standard worm handling protocols that can easily be adapted by other laboratories using C. elegans.

A Simple Microfluidic Chip for Long-Term Growth and Imaging of <em>Caenorhabditis elegans</em>

Caenorhabditis elegans (C. elegans) have proved to be a valuable model system for studying developmental and cell biological processes. Understanding these biological processes often requires long-term and repeated imaging of the same animal. Long recovery times associated with conventional immobilization methods done on agar pads have detrimental effects on animal health making it inappropriate to repeatedly image the same animal over long periods of time. This paper describes a microfluidic chip design, fabrication method, on-chip C. elegans culturing protocol, and three examples of long-term imaging to study developmental processes in individual animals. The chip, fabricated with polydimethylsiloxane and bonded on a cover glass, immobilizes animals on a glass substrate using an elastomeric membrane that is deflected using nitrogen gas. Complete immobilization of C. elegans enables robust time-lapse imaging of cellular and sub-cellular events in an anesthetic-free manner. A channel geometry with a large cross-section allows the animal to move freely within two partially sealed isolation membranes permitting growth in the channel with a continuous food supply. Using this simple chip, imaging of developmental phenomena such as neuronal process growth, vulval development, and dendritic arborization in the PVD sensory neurons, as the animal grows inside the channel, can be performed. The long-term growth and imaging chip operates with a single pressure line, no external valves, inexpensive fluidic consumables, and utilizes standard worm handling protocols that can easily be adapted by other laboratories using C. elegans.

Tradeoffs associated with autotomy and regeneration and their potential role in the evolution of regenerative abilities

Abstract The capacity of certain animals to regrow a lost appendage has been exploited as a powerful tool to study development. As a result, we now understand many of the proximate details of the regeneration process. Ironically, despite being one of the oldest studied developmental phenomena, regeneration is not often considered in the context of natural selection and evolution. Why do select species retain the capacity to shed and regrow body parts, whereas more derived lineages do not? We conducted a comprehensive study on the costs and benefits of autotomy and regeneration on Hemigrapsus nudus, the purple shore crab. In the realms of feeding and locomotion, regeneration restored fitness to what it otherwise would have been; autotomized animals showed decreases in feeding and locomotion, but regenerated animals performed no differently than intact crabs. However, for fecundity and male–male competition, regenerated animals had the lowest fitness compared with control and autotomized crabs. Our results raise the intriguing possibility that tradeoffs associated with reproduction may have led to the loss of regenerative abilities in derived lineages such as mammals and birds. Future work on the hundreds of species that regenerate lost body parts will reveal if and how this hypothesis can address the pervasive speculation plaguing the ultimate causes of regenerative losses.

Oral RNAi for Gene Silencing in Mosquitoes: From the Bench to the Field.

RNA interference (RNAi) has played a key role in the field of insect functional genomics, a discipline that has enhanced the study of developmental, evolutionary, physiological, and molecular biological phenomena in a wide variety of insects, including disease vector mosquitoes. Here we introduce a recently optimized RNAi procedure in which adult mosquitoes are fed with a colored sugar bait containing small interfering RNA (siRNA). This procedure effectively and economically leads to gene silencing, is technically straightforward, and has been successfully used to characterize a number of genes in adult mosquitoes. We also discuss how, in addition to laboratory applications, this oral RNAi procedure might one day be used in the field for controlling insect pests.

Underdiagnosed food allergies in children on Medicaid

Morphogenesis, the coming-into-being of living organisms, was first described in the 4th century BC by Aristotle, progenitor of biology and embryology. Over the centuries it has been the subject of innumerable commentaries by philosophers, theologians and scientists but no consensus has ever been reached as to its causes. In the late 19th century, along with the emergence of cellular and molecular biology, embryology underwent a renaissance and became a topic of great interest and research. Early on the discipline divided into two opposing factions, those who attempted to explain fetal development on the basis of cellular and molecular mechanisms, and those who invoked the presence of organizing fields. The morphogenic field was first articulated in the early decades of the 20th century by multiple researchers independently of each other. The field became an extremely useful conceptual tool by which to explain a wide range of developmental phenomena. While embryology and genetics originally formed a unified discipline, during the 1930s and 1940s geneticists became progressively skeptical of the field notion. The discovery of the DNA structure by Watson and Crick in the early 1950s decisively settled matters and thereafter the two disciplines pursued different lines of inquiry. After World War II embryology and the field concept went into a decades-long decline. By the 1980s an increasing number of scientists began to critically reexamine the morphogenic field concept and it underwent a second renaissance. In this paper I examine the development and evolution of the field concept, both experimentally and conceptually, and highlight the failure of genetic mechanisms to explain morphogenesis. I provide three instances from the medical literature of developmental phenomena which are only explainable on the basis of morphogenic field dynamics and argue that the field concept must be readmitted into mainstream scientific discourse.

Phenopype: A phenotyping pipeline for Python

Abstract Digital images are an intuitive way to capture, store and analyse organismal phenotypes. Many biologists are taking images to collect high‐dimensional phenotypic information from specimens to investigate complex ecological, evolutionary and developmental phenomena, such as relationships between trait diversity and ecosystem function, multivariate natural selection or developmental plasticity. As a consequence, images are being collected at ever‐increasing rates, but extraction of the contained phenotypic information poses a veritable analytical bottleneck. phenopype is a high‐throughput phenotyping pipeline for the programming language Python that aims at alleviating this bottleneck. The package facilitates immediate extraction of high‐dimensional phenotypic data from digital images with low levels of background noise and complexity. At the core, phenopype provides functions for rapid signal processing‐based image preprocessing and segmentation, data extraction, as well as visualization and data export. This functionality is provided by wrapping low‐level computer vision libraries (such as OpenCV) into accessible functions to facilitate scientific image analysis. In addition, phenopype provides a project management ecosystem to streamline data collection and to increase reproducibility. phenopype offers two different workflows that support users during different stages of scientific image analysis. The low‐throughput workflow uses regular Python syntax and has greater flexibility at the cost of reproducibility, which is suitable for prototyping during the initial stages of a research project. The high‐throughput workflow allows users to specify and store image‐specific settings for analysis in human‐readable YAML format, and then execute all functions in one step by means of an interactive parser. This approach facilitates rapid program‐user interactions during batch processing, and greatly increases scientific reproducibility. Overall, phenopype intends to make the features of powerful but technically involved low‐level CV libraries available to biologists with little or no Python coding experience. Therefore, phenopype is aiming to augment, rather than replace the utility of existing Python CV libraries, allowing biologists to focus on rapid and reproducible data collection. Furthermore, image annotations produced by phenopype can be used as training data, thus presenting a stepping stone towards the application of deep learning architectures.

Evolving networks of human intelligence

Twentieth century theory formation in human intelligence was dominated by factor theories; network theories will dominate the twenty first. Network theories answer a broad call for formal theories in psychological science, provide a strong approach to an idiographic science, and create an opportunity to study the developmental mechanisms of human's cognitive dynamics. Although the current century already delivered two formal stationary network theories of human intelligence—mutualism and wired intelligence—integrating dynamic mechanisms remains a serious challenge. This challenge translates into clear priorities: the identification of robust developmental phenomena, the study of the biological and cognitive mechanisms that drive these phenomena, the integration of these mechanisms into network theories of growth, the integration of network theories from different explanatory levels, and the empirical characterization of the structure of network theories.