Sulfate-reducing bacteria (Desulfomicrobium baculatum) is a group of anaerobic bacteria capable of producing H2S gas, which sours crude oil, corrodes metal equipment, pipeline systems, and even forms biofilms that cause blockages reservoir, reducing the reception capacity of pumped water in oil and gas exploitation. Currently, the biocides are mainly aldehydes or cyclic amines combined with cationic active substances, which are very toxic to humans and the environment. The development of materials technology has created new nano-sized materials that are capable of replacing traditional biocides and are environmentally friendly. This study has shown that silver and copper metal nanoparticles with an average size of 50 nm and concentration from 500 ppm can inhibit and kill both sulfate-reducing bacteria strains swimming and adhering in the water at normal and high temperature conditions.